This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.72 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 303,425 (10.7%) Reads that were too short: 5,705 (0.2%) Reads written (passing filters): 297,720 (10.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 63,542,330 bp (7.4%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 303425 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1452 692.0 0 1452 7 61724 692.0 0 61724 8 57572 692.0 0 57572 9 41 692.0 0 41 10 11 692.0 0 11 11 75 692.0 0 75 12 54 692.0 0 54 13 185 692.0 0 185 14 10 692.0 0 10 15 7 692.0 0 7 16 8 692.0 0 8 17 11 692.0 0 11 18 10 692.0 0 10 19 12 692.0 0 12 20 11 692.0 0 11 21 15 692.0 0 15 22 11 692.0 0 11 23 6 692.0 0 6 24 15 692.0 0 15 25 27 692.0 0 27 26 13 692.0 0 13 27 23 692.0 0 23 28 15 692.0 0 15 29 4 692.0 0 4 30 5 692.0 0 5 31 26 692.0 0 26 32 11 692.0 0 11 33 18 692.0 0 18 34 24 692.0 0 24 35 10 692.0 0 10 36 14 692.0 0 14 37 29 692.0 0 29 38 37 692.0 0 37 39 11 692.0 0 11 40 24 692.0 0 24 41 12 692.0 0 12 42 14 692.0 0 14 43 22 692.0 0 22 44 20 692.0 0 20 45 41 692.0 0 41 46 4 692.0 0 4 47 21 692.0 0 21 48 7 692.0 0 7 49 2 692.0 0 2 50 9 692.0 0 9 51 23 692.0 0 23 52 7 692.0 0 7 53 10 692.0 0 10 54 11 692.0 0 11 55 7 692.0 0 7 56 18 692.0 0 18 57 6 692.0 0 6 58 14 692.0 0 14 59 20 692.0 0 20 60 20 692.0 0 20 61 10 692.0 0 10 62 5 692.0 0 5 63 10 692.0 0 10 64 7 692.0 0 7 65 8 692.0 0 8 66 17 692.0 0 17 67 11 692.0 0 11 68 6 692.0 0 6 69 13 692.0 0 13 70 19 692.0 0 19 71 28 692.0 0 28 72 100 692.0 0 100 73 34 692.0 0 34 74 272 692.0 0 272 75 38 692.0 0 38 76 10 692.0 0 10 77 11 692.0 0 11 78 20 692.0 0 20 79 13 692.0 0 13 80 34 692.0 0 34 81 19 692.0 0 19 82 19 692.0 0 19 83 19 692.0 0 19 84 17 692.0 0 17 85 15 692.0 0 15 86 12 692.0 0 12 87 70 692.0 0 70 88 14 692.0 0 14 89 30 692.0 0 30 90 28 692.0 0 28 91 35 692.0 0 35 92 22 692.0 0 22 93 35 692.0 0 35 94 24 692.0 0 24 95 12 692.0 0 12 96 48 692.0 0 48 97 49 692.0 0 49 98 69 692.0 0 69 99 86 692.0 0 86 100 48 692.0 0 48 101 13 692.0 0 13 102 75 692.0 0 75 103 48 692.0 0 48 104 56 692.0 0 56 105 106 692.0 0 106 106 53 692.0 0 53 107 89 692.0 0 89 108 81 692.0 0 81 109 43 692.0 0 43 110 69 692.0 0 69 111 56 692.0 0 56 112 126 692.0 0 126 113 69 692.0 0 69 114 50 692.0 0 50 115 381 692.0 0 381 116 39 692.0 0 39 117 23 692.0 0 23 118 43 692.0 0 43 119 36 692.0 0 36 120 29 692.0 0 29 121 51 692.0 0 51 122 256 692.0 0 256 123 134 692.0 0 134 124 310 692.0 0 310 125 497 692.0 0 497 126 138 692.0 0 138 127 279 692.0 0 279 128 134 692.0 0 134 129 149 692.0 0 149 130 295 692.0 0 295 131 153 692.0 0 153 132 159 692.0 0 159 133 105 692.0 0 105 134 153 692.0 0 153 135 280 692.0 0 280 136 346 692.0 0 346 137 584 692.0 0 584 138 1006 692.0 0 1006 139 1132 692.0 0 1132 140 2511 692.0 0 2511 141 4081 692.0 0 4081 142 79232 692.0 0 79232 143 22338 692.0 0 22338 144 48289 692.0 0 48289 145 223 692.0 0 223 146 57 692.0 0 57 147 76 692.0 0 76 148 55 692.0 0 55 149 56 692.0 0 56 150 45 692.0 0 45 151 52 692.0 0 52 152 35 692.0 0 35 153 31 692.0 0 31 154 32 692.0 0 32 155 50 692.0 0 50 156 46 692.0 0 46 157 66 692.0 0 66 158 61 692.0 0 61 159 76 692.0 0 76 160 174 692.0 0 174 161 309 692.0 0 309 162 5068 692.0 0 5068 163 941 692.0 0 941 164 1643 692.0 0 1643 165 30 692.0 0 30 166 21 692.0 0 21 167 46 692.0 0 46 168 37 692.0 0 37 169 47 692.0 0 47 170 38 692.0 0 38 171 20 692.0 0 20 172 18 692.0 0 18 173 12 692.0 0 12 174 11 692.0 0 11 175 34 692.0 0 34 176 49 692.0 0 49 177 92 692.0 0 92 178 45 692.0 0 45 179 28 692.0 0 28 180 19 692.0 0 19 181 24 692.0 0 24 182 18 692.0 0 18 183 29 692.0 0 29 184 32 692.0 0 32 185 26 692.0 0 26 186 20 692.0 0 20 187 29 692.0 0 29 188 221 692.0 0 221 189 51 692.0 0 51 190 120 692.0 0 120 191 15 692.0 0 15 192 9 692.0 0 9 193 22 692.0 0 22 194 25 692.0 0 25 195 25 692.0 0 25 196 29 692.0 0 29 197 34 692.0 0 34 198 30 692.0 0 30 199 32 692.0 0 32 200 20 692.0 0 20 201 23 692.0 0 23 202 35 692.0 0 35 203 31 692.0 0 31 204 31 692.0 0 31 205 29 692.0 0 29 206 35 692.0 0 35 207 37 692.0 0 37 208 36 692.0 0 36 209 47 692.0 0 47 210 52 692.0 0 52 211 49 692.0 0 49 212 59 692.0 0 59 213 53 692.0 0 53 214 265 692.0 0 265 215 170 692.0 0 170 216 77 692.0 0 77 217 43 692.0 0 43 218 35 692.0 0 35 219 25 692.0 0 25 220 23 692.0 0 23 221 30 692.0 0 30 222 63 692.0 0 63 223 63 692.0 0 63 224 75 692.0 0 75 225 89 692.0 0 89 226 69 692.0 0 69 227 34 692.0 0 34 228 11 692.0 0 11 229 22 692.0 0 22 230 24 692.0 0 24 231 23 692.0 0 23 232 116 692.0 0 116 233 71 692.0 0 71 234 164 692.0 0 164 235 33 692.0 0 33 236 29 692.0 0 29 237 35 692.0 0 35 238 44 692.0 0 44 239 38 692.0 0 38 240 40 692.0 0 40 241 79 692.0 0 79 242 36 692.0 0 36 243 31 692.0 0 31 244 26 692.0 0 26 245 45 692.0 0 45 246 35 692.0 0 35 247 41 692.0 0 41 248 24 692.0 0 24 249 26 692.0 0 26 250 30 692.0 0 30 251 30 692.0 0 30 252 32 692.0 0 32 253 34 692.0 0 34 254 33 692.0 0 33 255 47 692.0 0 47 256 54 692.0 0 54 257 65 692.0 0 65 258 50 692.0 0 50 259 49 692.0 0 49 260 55 692.0 0 55 261 59 692.0 0 59 262 110 692.0 0 110 263 175 692.0 0 175 264 139 692.0 0 139 265 42 692.0 0 42 266 35 692.0 0 35 267 28 692.0 0 28 268 46 692.0 0 46 269 56 692.0 0 56 270 106 692.0 0 106 271 59 692.0 0 59 272 31 692.0 0 31 273 42 692.0 0 42 274 53 692.0 0 53 275 59 692.0 0 59 276 119 692.0 0 119 277 46 692.0 0 46 278 37 692.0 0 37 279 70 692.0 0 70 280 79 692.0 0 79 281 219 692.0 0 219 282 67 692.0 0 67 283 95 692.0 0 95 284 60 692.0 0 60 285 62 692.0 0 62 286 45 692.0 0 45 287 50 692.0 0 50 288 37 692.0 0 37 289 32 692.0 0 32 290 40 692.0 0 40 291 40 692.0 0 40 292 62 692.0 0 62 293 31 692.0 0 31 294 52 692.0 0 52 295 67 692.0 0 67 296 56 692.0 0 56 297 105 692.0 0 105 298 57 692.0 0 57 299 26 692.0 0 26 300 42 692.0 0 42 301 42 692.0 0 42 Finished in 12.48 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 297,720 Reads with adapters: 59,201 (19.9%) Reads that were too short: 54 (0.0%) Reads written (passing filters): 59,147 (19.9%) Total basepairs processed: 63,542,330 bp Total written (filtered): 16,137,994 bp (25.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 59201 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 3 0.0 3 0 0 0 2 1 18 13 0.0 3 0 0 5 3 5 19 361 0.0 3 0 230 72 43 16 20 58345 0.0 4 54910 2785 477 130 43 21 140 0.0 4 20 111 8 0 1 22 10 0.0 4 0 1 3 5 1 23 175 0.0 4 1 0 0 129 45 24 1 0.0 4 1 25 1 0.0 4 0 0 0 0 1 28 2 0.0 4 0 1 0 0 1 30 1 0.0 4 0 0 0 0 1 37 6 0.0 4 0 0 0 0 6 38 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 66 0.0 4 0 0 0 0 66 128 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 139 4 0.0 4 0 0 0 0 4 145 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 5 0.0 4 0 0 0 0 5 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 3 0.0 4 0 0 0 1 2 236 4 0.0 4 0 0 0 1 3 237 5 0.0 4 0 0 0 1 4 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 2 0.0 4 0 0 0 0 2 264 3 0.0 4 0 0 0 0 3 265 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 287 1 0.0 4 0 0 0 0 1 294 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.13 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 59,147 Reads with adapters: 10,714 (18.1%) Reads written (passing filters): 59,147 (100.0%) Total basepairs processed: 16,137,994 bp Total written (filtered): 16,015,092 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10714 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 9.7% G: 84.3% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 97 924.2 0 97 4 25 231.0 0 25 5 1 57.8 0 1 6 3 14.4 0 3 9 176 0.2 0 10 166 10 2023 0.1 1 207 1816 11 8178 0.0 1 367 7811 12 140 0.0 1 5 135 13 12 0.0 1 0 12 14 4 0.0 1 0 4 15 4 0.0 1 0 4 73 1 0.0 1 1 123 1 0.0 1 0 1 148 1 0.0 1 1 151 28 0.0 1 20 8 190 1 0.0 1 0 1 209 16 0.0 1 14 2 210 1 0.0 1 1 224 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.