This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.14 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 320,033 (11.3%) Reads that were too short: 4,315 (0.2%) Reads written (passing filters): 315,718 (11.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 66,857,941 bp (7.8%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 320033 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1127 692.0 0 1127 7 56150 692.0 0 56150 8 67265 692.0 0 67265 9 43 692.0 0 43 10 9 692.0 0 9 11 33 692.0 0 33 12 37 692.0 0 37 13 70 692.0 0 70 14 6 692.0 0 6 15 6 692.0 0 6 16 6 692.0 0 6 17 3 692.0 0 3 18 7 692.0 0 7 19 12 692.0 0 12 20 16 692.0 0 16 21 16 692.0 0 16 22 13 692.0 0 13 23 3 692.0 0 3 24 7 692.0 0 7 25 4 692.0 0 4 26 8 692.0 0 8 27 17 692.0 0 17 28 14 692.0 0 14 29 2 692.0 0 2 30 3 692.0 0 3 31 10 692.0 0 10 32 4 692.0 0 4 33 9 692.0 0 9 34 27 692.0 0 27 35 10 692.0 0 10 36 4 692.0 0 4 37 9 692.0 0 9 38 4 692.0 0 4 39 11 692.0 0 11 40 7 692.0 0 7 41 7 692.0 0 7 42 13 692.0 0 13 43 27 692.0 0 27 44 21 692.0 0 21 45 44 692.0 0 44 46 9 692.0 0 9 47 16 692.0 0 16 48 2 692.0 0 2 49 4 692.0 0 4 50 8 692.0 0 8 51 30 692.0 0 30 52 6 692.0 0 6 53 5 692.0 0 5 54 16 692.0 0 16 55 13 692.0 0 13 56 9 692.0 0 9 57 11 692.0 0 11 58 30 692.0 0 30 59 16 692.0 0 16 60 14 692.0 0 14 61 7 692.0 0 7 62 9 692.0 0 9 63 2 692.0 0 2 64 4 692.0 0 4 65 11 692.0 0 11 66 12 692.0 0 12 67 9 692.0 0 9 68 2 692.0 0 2 69 10 692.0 0 10 70 20 692.0 0 20 71 35 692.0 0 35 72 92 692.0 0 92 73 19 692.0 0 19 74 278 692.0 0 278 75 18 692.0 0 18 76 3 692.0 0 3 77 13 692.0 0 13 78 20 692.0 0 20 79 10 692.0 0 10 80 13 692.0 0 13 81 15 692.0 0 15 82 26 692.0 0 26 83 18 692.0 0 18 84 19 692.0 0 19 85 22 692.0 0 22 86 13 692.0 0 13 87 60 692.0 0 60 88 22 692.0 0 22 89 63 692.0 0 63 90 34 692.0 0 34 91 69 692.0 0 69 92 46 692.0 0 46 93 67 692.0 0 67 94 17 692.0 0 17 95 21 692.0 0 21 96 26 692.0 0 26 97 27 692.0 0 27 98 60 692.0 0 60 99 49 692.0 0 49 100 29 692.0 0 29 101 27 692.0 0 27 102 64 692.0 0 64 103 40 692.0 0 40 104 45 692.0 0 45 105 124 692.0 0 124 106 82 692.0 0 82 107 83 692.0 0 83 108 82 692.0 0 82 109 64 692.0 0 64 110 51 692.0 0 51 111 57 692.0 0 57 112 109 692.0 0 109 113 54 692.0 0 54 114 78 692.0 0 78 115 394 692.0 0 394 116 43 692.0 0 43 117 22 692.0 0 22 118 27 692.0 0 27 119 24 692.0 0 24 120 47 692.0 0 47 121 37 692.0 0 37 122 277 692.0 0 277 123 106 692.0 0 106 124 306 692.0 0 306 125 548 692.0 0 548 126 153 692.0 0 153 127 276 692.0 0 276 128 144 692.0 0 144 129 170 692.0 0 170 130 326 692.0 0 326 131 150 692.0 0 150 132 150 692.0 0 150 133 116 692.0 0 116 134 185 692.0 0 185 135 283 692.0 0 283 136 298 692.0 0 298 137 540 692.0 0 540 138 978 692.0 0 978 139 1167 692.0 0 1167 140 2494 692.0 0 2494 141 3997 692.0 0 3997 142 86149 692.0 0 86149 143 24304 692.0 0 24304 144 54304 692.0 0 54304 145 230 692.0 0 230 146 39 692.0 0 39 147 66 692.0 0 66 148 34 692.0 0 34 149 49 692.0 0 49 150 47 692.0 0 47 151 31 692.0 0 31 152 27 692.0 0 27 153 37 692.0 0 37 154 22 692.0 0 22 155 69 692.0 0 69 156 38 692.0 0 38 157 67 692.0 0 67 158 63 692.0 0 63 159 74 692.0 0 74 160 139 692.0 0 139 161 315 692.0 0 315 162 4938 692.0 0 4938 163 930 692.0 0 930 164 1716 692.0 0 1716 165 20 692.0 0 20 166 15 692.0 0 15 167 42 692.0 0 42 168 36 692.0 0 36 169 37 692.0 0 37 170 22 692.0 0 22 171 14 692.0 0 14 172 14 692.0 0 14 173 16 692.0 0 16 174 8 692.0 0 8 175 21 692.0 0 21 176 46 692.0 0 46 177 71 692.0 0 71 178 43 692.0 0 43 179 29 692.0 0 29 180 5 692.0 0 5 181 15 692.0 0 15 182 17 692.0 0 17 183 19 692.0 0 19 184 23 692.0 0 23 185 27 692.0 0 27 186 24 692.0 0 24 187 27 692.0 0 27 188 223 692.0 0 223 189 47 692.0 0 47 190 147 692.0 0 147 191 13 692.0 0 13 192 11 692.0 0 11 193 22 692.0 0 22 194 17 692.0 0 17 195 37 692.0 0 37 196 32 692.0 0 32 197 32 692.0 0 32 198 22 692.0 0 22 199 22 692.0 0 22 200 16 692.0 0 16 201 17 692.0 0 17 202 30 692.0 0 30 203 25 692.0 0 25 204 26 692.0 0 26 205 22 692.0 0 22 206 37 692.0 0 37 207 37 692.0 0 37 208 30 692.0 0 30 209 38 692.0 0 38 210 43 692.0 0 43 211 30 692.0 0 30 212 33 692.0 0 33 213 32 692.0 0 32 214 158 692.0 0 158 215 69 692.0 0 69 216 62 692.0 0 62 217 32 692.0 0 32 218 22 692.0 0 22 219 17 692.0 0 17 220 19 692.0 0 19 221 28 692.0 0 28 222 51 692.0 0 51 223 44 692.0 0 44 224 37 692.0 0 37 225 81 692.0 0 81 226 95 692.0 0 95 227 41 692.0 0 41 228 21 692.0 0 21 229 45 692.0 0 45 230 19 692.0 0 19 231 21 692.0 0 21 232 60 692.0 0 60 233 37 692.0 0 37 234 62 692.0 0 62 235 40 692.0 0 40 236 35 692.0 0 35 237 30 692.0 0 30 238 32 692.0 0 32 239 32 692.0 0 32 240 36 692.0 0 36 241 71 692.0 0 71 242 39 692.0 0 39 243 33 692.0 0 33 244 26 692.0 0 26 245 35 692.0 0 35 246 31 692.0 0 31 247 24 692.0 0 24 248 18 692.0 0 18 249 17 692.0 0 17 250 27 692.0 0 27 251 37 692.0 0 37 252 33 692.0 0 33 253 22 692.0 0 22 254 22 692.0 0 22 255 41 692.0 0 41 256 42 692.0 0 42 257 72 692.0 0 72 258 44 692.0 0 44 259 50 692.0 0 50 260 56 692.0 0 56 261 49 692.0 0 49 262 71 692.0 0 71 263 135 692.0 0 135 264 59 692.0 0 59 265 31 692.0 0 31 266 27 692.0 0 27 267 24 692.0 0 24 268 28 692.0 0 28 269 39 692.0 0 39 270 65 692.0 0 65 271 39 692.0 0 39 272 27 692.0 0 27 273 35 692.0 0 35 274 25 692.0 0 25 275 44 692.0 0 44 276 56 692.0 0 56 277 40 692.0 0 40 278 27 692.0 0 27 279 53 692.0 0 53 280 60 692.0 0 60 281 192 692.0 0 192 282 67 692.0 0 67 283 57 692.0 0 57 284 43 692.0 0 43 285 32 692.0 0 32 286 31 692.0 0 31 287 33 692.0 0 33 288 23 692.0 0 23 289 32 692.0 0 32 290 36 692.0 0 36 291 24 692.0 0 24 292 48 692.0 0 48 293 31 692.0 0 31 294 49 692.0 0 49 295 51 692.0 0 51 296 57 692.0 0 57 297 100 692.0 0 100 298 37 692.0 0 37 299 26 692.0 0 26 300 43 692.0 0 43 301 20 692.0 0 20 Finished in 11.83 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 315,718 Reads with adapters: 55,654 (17.6%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 55,608 (17.6%) Total basepairs processed: 66,857,941 bp Total written (filtered): 15,402,175 bp (23.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 55654 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 219 0.0 3 0 211 5 3 17 55196 0.0 3 54170 992 33 1 18 124 0.0 3 10 111 2 1 19 20 0.0 3 0 0 19 1 20 40 0.0 3 0 0 0 40 32 1 0.0 3 1 40 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 7 0.0 3 0 0 0 7 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 1 118 1 0.0 3 0 0 1 138 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 2 0.0 3 0 0 1 1 151 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 1 207 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 3 0.0 3 0 0 1 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.97 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 55,608 Reads with adapters: 169 (0.3%) Reads written (passing filters): 55,608 (100.0%) Total basepairs processed: 15,402,175 bp Total written (filtered): 15,401,644 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 169 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.2% C: 50.3% G: 16.0% T: 6.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 150 868.9 0 150 4 14 217.2 0 14 5 5 54.3 0 5