This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.83 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 113,731 (4.0%) Reads that were too short: 21,273 (0.8%) Reads written (passing filters): 92,458 (3.3%) Total basepairs processed: 853,219,115 bp Total written (filtered): 22,445,784 bp (2.6%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 113731 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 512 692.0 0 512 7 27714 692.0 0 27714 8 826 692.0 0 826 9 27785 692.0 0 27785 10 78 692.0 0 78 11 55 692.0 0 55 12 50 692.0 0 50 13 63 692.0 0 63 14 51 692.0 0 51 15 42 692.0 0 42 16 42 692.0 0 42 17 56 692.0 0 56 18 44 692.0 0 44 19 36 692.0 0 36 20 78 692.0 0 78 21 72 692.0 0 72 22 59 692.0 0 59 23 67 692.0 0 67 24 49 692.0 0 49 25 58 692.0 0 58 26 54 692.0 0 54 27 67 692.0 0 67 28 61 692.0 0 61 29 92 692.0 0 92 30 69 692.0 0 69 31 372 692.0 0 372 32 455 692.0 0 455 33 967 692.0 0 967 34 55 692.0 0 55 35 43 692.0 0 43 36 40 692.0 0 40 37 75 692.0 0 75 38 49 692.0 0 49 39 42 692.0 0 42 40 51 692.0 0 51 41 59 692.0 0 59 42 51 692.0 0 51 43 47 692.0 0 47 44 46 692.0 0 46 45 35 692.0 0 35 46 44 692.0 0 44 47 63 692.0 0 63 48 37 692.0 0 37 49 40 692.0 0 40 50 54 692.0 0 54 51 91 692.0 0 91 52 72 692.0 0 72 53 37 692.0 0 37 54 43 692.0 0 43 55 51 692.0 0 51 56 54 692.0 0 54 57 51 692.0 0 51 58 52 692.0 0 52 59 52 692.0 0 52 60 49 692.0 0 49 61 36 692.0 0 36 62 45 692.0 0 45 63 50 692.0 0 50 64 79 692.0 0 79 65 57 692.0 0 57 66 91 692.0 0 91 67 98 692.0 0 98 68 744 692.0 0 744 69 569 692.0 0 569 70 300 692.0 0 300 71 46 692.0 0 46 72 49 692.0 0 49 73 121 692.0 0 121 74 92 692.0 0 92 75 69 692.0 0 69 76 57 692.0 0 57 77 75 692.0 0 75 78 62 692.0 0 62 79 109 692.0 0 109 80 107 692.0 0 107 81 69 692.0 0 69 82 93 692.0 0 93 83 91 692.0 0 91 84 66 692.0 0 66 85 68 692.0 0 68 86 75 692.0 0 75 87 76 692.0 0 76 88 84 692.0 0 84 89 87 692.0 0 87 90 50 692.0 0 50 91 58 692.0 0 58 92 74 692.0 0 74 93 53 692.0 0 53 94 56 692.0 0 56 95 73 692.0 0 73 96 68 692.0 0 68 97 64 692.0 0 64 98 126 692.0 0 126 99 155 692.0 0 155 100 120 692.0 0 120 101 76 692.0 0 76 102 58 692.0 0 58 103 61 692.0 0 61 104 55 692.0 0 55 105 67 692.0 0 67 106 74 692.0 0 74 107 60 692.0 0 60 108 63 692.0 0 63 109 63 692.0 0 63 110 72 692.0 0 72 111 127 692.0 0 127 112 59 692.0 0 59 113 105 692.0 0 105 114 79 692.0 0 79 115 72 692.0 0 72 116 451 692.0 0 451 117 220 692.0 0 220 118 381 692.0 0 381 119 82 692.0 0 82 120 88 692.0 0 88 121 98 692.0 0 98 122 132 692.0 0 132 123 165 692.0 0 165 124 102 692.0 0 102 125 71 692.0 0 71 126 167 692.0 0 167 127 89 692.0 0 89 128 90 692.0 0 90 129 58 692.0 0 58 130 120 692.0 0 120 131 149 692.0 0 149 132 223 692.0 0 223 133 324 692.0 0 324 134 553 692.0 0 553 135 698 692.0 0 698 136 1114 692.0 0 1114 137 192 692.0 0 192 138 239 692.0 0 239 139 523 692.0 0 523 140 61 692.0 0 61 141 78 692.0 0 78 142 110 692.0 0 110 143 89 692.0 0 89 144 150 692.0 0 150 145 93 692.0 0 93 146 183 692.0 0 183 147 91 692.0 0 91 148 104 692.0 0 104 149 77 692.0 0 77 150 99 692.0 0 99 151 79 692.0 0 79 152 147 692.0 0 147 153 131 692.0 0 131 154 191 692.0 0 191 155 232 692.0 0 232 156 230 692.0 0 230 157 618 692.0 0 618 158 121 692.0 0 121 159 148 692.0 0 148 160 106 692.0 0 106 161 592 692.0 0 592 162 280 692.0 0 280 163 112 692.0 0 112 164 488 692.0 0 488 165 102 692.0 0 102 166 618 692.0 0 618 167 184 692.0 0 184 168 150 692.0 0 150 169 132 692.0 0 132 170 83 692.0 0 83 171 104 692.0 0 104 172 89 692.0 0 89 173 71 692.0 0 71 174 112 692.0 0 112 175 279 692.0 0 279 176 284 692.0 0 284 177 867 692.0 0 867 178 162 692.0 0 162 179 200 692.0 0 200 180 691 692.0 0 691 181 477 692.0 0 477 182 1102 692.0 0 1102 183 443 692.0 0 443 184 148 692.0 0 148 185 92 692.0 0 92 186 122 692.0 0 122 187 144 692.0 0 144 188 163 692.0 0 163 189 108 692.0 0 108 190 111 692.0 0 111 191 100 692.0 0 100 192 87 692.0 0 87 193 144 692.0 0 144 194 193 692.0 0 193 195 541 692.0 0 541 196 725 692.0 0 725 197 638 692.0 0 638 198 201 692.0 0 201 199 156 692.0 0 156 200 2112 692.0 0 2112 201 2774 692.0 0 2774 202 5571 692.0 0 5571 203 639 692.0 0 639 204 50 692.0 0 50 205 72 692.0 0 72 206 57 692.0 0 57 207 76 692.0 0 76 208 66 692.0 0 66 209 73 692.0 0 73 210 53 692.0 0 53 211 69 692.0 0 69 212 69 692.0 0 69 213 71 692.0 0 71 214 102 692.0 0 102 215 60 692.0 0 60 216 74 692.0 0 74 217 73 692.0 0 73 218 57 692.0 0 57 219 67 692.0 0 67 220 107 692.0 0 107 221 257 692.0 0 257 222 271 692.0 0 271 223 292 692.0 0 292 224 116 692.0 0 116 225 87 692.0 0 87 226 141 692.0 0 141 227 66 692.0 0 66 228 64 692.0 0 64 229 51 692.0 0 51 230 78 692.0 0 78 231 65 692.0 0 65 232 58 692.0 0 58 233 83 692.0 0 83 234 71 692.0 0 71 235 72 692.0 0 72 236 65 692.0 0 65 237 66 692.0 0 66 238 109 692.0 0 109 239 77 692.0 0 77 240 162 692.0 0 162 241 140 692.0 0 140 242 203 692.0 0 203 243 177 692.0 0 177 244 134 692.0 0 134 245 55 692.0 0 55 246 62 692.0 0 62 247 58 692.0 0 58 248 75 692.0 0 75 249 90 692.0 0 90 250 115 692.0 0 115 251 371 692.0 0 371 252 125 692.0 0 125 253 145 692.0 0 145 254 147 692.0 0 147 255 237 692.0 0 237 256 143 692.0 0 143 257 136 692.0 0 136 258 190 692.0 0 190 259 153 692.0 0 153 260 239 692.0 0 239 261 101 692.0 0 101 262 191 692.0 0 191 263 274 692.0 0 274 264 1065 692.0 0 1065 265 727 692.0 0 727 266 447 692.0 0 447 267 58 692.0 0 58 268 142 692.0 0 142 269 202 692.0 0 202 270 138 692.0 0 138 271 100 692.0 0 100 272 95 692.0 0 95 273 80 692.0 0 80 274 195 692.0 0 195 275 250 692.0 0 250 276 1015 692.0 0 1015 277 648 692.0 0 648 278 595 692.0 0 595 279 61 692.0 0 61 280 51 692.0 0 51 281 61 692.0 0 61 282 72 692.0 0 72 283 66 692.0 0 66 284 66 692.0 0 66 285 67 692.0 0 67 286 58 692.0 0 58 287 56 692.0 0 56 288 62 692.0 0 62 289 95 692.0 0 95 290 114 692.0 0 114 291 75 692.0 0 75 292 115 692.0 0 115 293 68 692.0 0 68 294 85 692.0 0 85 295 95 692.0 0 95 296 106 692.0 0 106 297 105 692.0 0 105 298 158 692.0 0 158 299 166 692.0 0 166 300 166 692.0 0 166 301 330 692.0 0 330 Finished in 4.50 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 92,458 Reads with adapters: 27,530 (29.8%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 27,487 (29.7%) Total basepairs processed: 22,445,784 bp Total written (filtered): 7,528,301 bp (33.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27530 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 5 0.0 3 0 0 0 3 2 19 143 0.0 3 0 105 17 14 7 20 27225 0.0 4 25901 1085 174 51 14 21 48 0.0 4 10 37 1 22 8 0.0 4 1 1 2 1 3 23 27 0.0 4 1 0 0 20 6 24 3 0.0 4 0 0 0 0 3 25 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 1 36 1 0.0 4 0 0 0 0 1 40 4 0.0 4 0 0 0 0 4 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 1 69 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 107 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 5 0.0 4 0 0 0 0 5 129 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 135 1 0.0 4 1 149 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 178 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.94 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 27,487 Reads with adapters: 1,484 (5.4%) Reads written (passing filters): 27,487 (100.0%) Total basepairs processed: 7,528,301 bp Total written (filtered): 7,495,969 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1484 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.4% C: 13.3% G: 71.4% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 142 429.5 0 142 4 8 107.4 0 8 5 1 26.8 0 1 8 1 0.4 0 1 9 9 0.1 0 1 8 10 22 0.0 1 1 21 11 761 0.0 1 47 714 12 435 0.0 1 17 418 13 16 0.0 1 2 14 16 1 0.0 1 0 1 73 2 0.0 1 1 1 74 2 0.0 1 2 93 1 0.0 1 0 1 140 1 0.0 1 0 1 149 1 0.0 1 1 152 13 0.0 1 8 5 153 1 0.0 1 1 171 1 0.0 1 1 210 7 0.0 1 6 1 211 1 0.0 1 0 1 225 56 0.0 1 49 7 226 1 0.0 1 1 262 1 0.0 1 1