This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.75 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 98,145 (3.5%) Reads that were too short: 13,622 (0.5%) Reads written (passing filters): 84,523 (3.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 21,480,830 bp (2.5%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 98145 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 369 692.0 0 369 7 32951 692.0 0 32951 8 426 692.0 0 426 9 25515 692.0 0 25515 10 59 692.0 0 59 11 38 692.0 0 38 12 34 692.0 0 34 13 37 692.0 0 37 14 34 692.0 0 34 15 41 692.0 0 41 16 31 692.0 0 31 17 30 692.0 0 30 18 32 692.0 0 32 19 32 692.0 0 32 20 38 692.0 0 38 21 38 692.0 0 38 22 35 692.0 0 35 23 27 692.0 0 27 24 31 692.0 0 31 25 19 692.0 0 19 26 43 692.0 0 43 27 42 692.0 0 42 28 34 692.0 0 34 29 54 692.0 0 54 30 60 692.0 0 60 31 114 692.0 0 114 32 96 692.0 0 96 33 191 692.0 0 191 34 38 692.0 0 38 35 31 692.0 0 31 36 27 692.0 0 27 37 64 692.0 0 64 38 54 692.0 0 54 39 41 692.0 0 41 40 323 692.0 0 323 41 60 692.0 0 60 42 54 692.0 0 54 43 36 692.0 0 36 44 45 692.0 0 45 45 35 692.0 0 35 46 42 692.0 0 42 47 38 692.0 0 38 48 24 692.0 0 24 49 27 692.0 0 27 50 51 692.0 0 51 51 80 692.0 0 80 52 41 692.0 0 41 53 35 692.0 0 35 54 47 692.0 0 47 55 41 692.0 0 41 56 44 692.0 0 44 57 33 692.0 0 33 58 41 692.0 0 41 59 50 692.0 0 50 60 41 692.0 0 41 61 38 692.0 0 38 62 38 692.0 0 38 63 46 692.0 0 46 64 50 692.0 0 50 65 46 692.0 0 46 66 72 692.0 0 72 67 98 692.0 0 98 68 801 692.0 0 801 69 610 692.0 0 610 70 305 692.0 0 305 71 33 692.0 0 33 72 42 692.0 0 42 73 41 692.0 0 41 74 44 692.0 0 44 75 36 692.0 0 36 76 32 692.0 0 32 77 28 692.0 0 28 78 38 692.0 0 38 79 48 692.0 0 48 80 99 692.0 0 99 81 47 692.0 0 47 82 115 692.0 0 115 83 44 692.0 0 44 84 53 692.0 0 53 85 47 692.0 0 47 86 45 692.0 0 45 87 41 692.0 0 41 88 67 692.0 0 67 89 46 692.0 0 46 90 57 692.0 0 57 91 58 692.0 0 58 92 43 692.0 0 43 93 38 692.0 0 38 94 49 692.0 0 49 95 42 692.0 0 42 96 48 692.0 0 48 97 48 692.0 0 48 98 83 692.0 0 83 99 84 692.0 0 84 100 86 692.0 0 86 101 64 692.0 0 64 102 61 692.0 0 61 103 56 692.0 0 56 104 57 692.0 0 57 105 51 692.0 0 51 106 39 692.0 0 39 107 51 692.0 0 51 108 45 692.0 0 45 109 41 692.0 0 41 110 45 692.0 0 45 111 68 692.0 0 68 112 107 692.0 0 107 113 64 692.0 0 64 114 45 692.0 0 45 115 42 692.0 0 42 116 232 692.0 0 232 117 125 692.0 0 125 118 163 692.0 0 163 119 57 692.0 0 57 120 52 692.0 0 52 121 158 692.0 0 158 122 80 692.0 0 80 123 98 692.0 0 98 124 53 692.0 0 53 125 53 692.0 0 53 126 94 692.0 0 94 127 66 692.0 0 66 128 46 692.0 0 46 129 56 692.0 0 56 130 84 692.0 0 84 131 80 692.0 0 80 132 122 692.0 0 122 133 220 692.0 0 220 134 317 692.0 0 317 135 462 692.0 0 462 136 538 692.0 0 538 137 178 692.0 0 178 138 275 692.0 0 275 139 668 692.0 0 668 140 55 692.0 0 55 141 55 692.0 0 55 142 99 692.0 0 99 143 69 692.0 0 69 144 107 692.0 0 107 145 67 692.0 0 67 146 107 692.0 0 107 147 74 692.0 0 74 148 75 692.0 0 75 149 75 692.0 0 75 150 96 692.0 0 96 151 74 692.0 0 74 152 129 692.0 0 129 153 146 692.0 0 146 154 166 692.0 0 166 155 137 692.0 0 137 156 158 692.0 0 158 157 341 692.0 0 341 158 91 692.0 0 91 159 156 692.0 0 156 160 116 692.0 0 116 161 667 692.0 0 667 162 264 692.0 0 264 163 114 692.0 0 114 164 616 692.0 0 616 165 90 692.0 0 90 166 708 692.0 0 708 167 160 692.0 0 160 168 115 692.0 0 115 169 66 692.0 0 66 170 56 692.0 0 56 171 56 692.0 0 56 172 74 692.0 0 74 173 49 692.0 0 49 174 61 692.0 0 61 175 137 692.0 0 137 176 237 692.0 0 237 177 450 692.0 0 450 178 93 692.0 0 93 179 114 692.0 0 114 180 510 692.0 0 510 181 308 692.0 0 308 182 691 692.0 0 691 183 384 692.0 0 384 184 135 692.0 0 135 185 78 692.0 0 78 186 82 692.0 0 82 187 121 692.0 0 121 188 153 692.0 0 153 189 80 692.0 0 80 190 78 692.0 0 78 191 67 692.0 0 67 192 71 692.0 0 71 193 76 692.0 0 76 194 148 692.0 0 148 195 238 692.0 0 238 196 398 692.0 0 398 197 350 692.0 0 350 198 138 692.0 0 138 199 92 692.0 0 92 200 1525 692.0 0 1525 201 1587 692.0 0 1587 202 3103 692.0 0 3103 203 327 692.0 0 327 204 44 692.0 0 44 205 46 692.0 0 46 206 42 692.0 0 42 207 77 692.0 0 77 208 65 692.0 0 65 209 52 692.0 0 52 210 34 692.0 0 34 211 71 692.0 0 71 212 57 692.0 0 57 213 57 692.0 0 57 214 68 692.0 0 68 215 43 692.0 0 43 216 56 692.0 0 56 217 51 692.0 0 51 218 46 692.0 0 46 219 50 692.0 0 50 220 61 692.0 0 61 221 102 692.0 0 102 222 133 692.0 0 133 223 130 692.0 0 130 224 78 692.0 0 78 225 77 692.0 0 77 226 140 692.0 0 140 227 59 692.0 0 59 228 51 692.0 0 51 229 55 692.0 0 55 230 59 692.0 0 59 231 59 692.0 0 59 232 54 692.0 0 54 233 79 692.0 0 79 234 48 692.0 0 48 235 41 692.0 0 41 236 64 692.0 0 64 237 54 692.0 0 54 238 84 692.0 0 84 239 80 692.0 0 80 240 85 692.0 0 85 241 72 692.0 0 72 242 85 692.0 0 85 243 88 692.0 0 88 244 67 692.0 0 67 245 52 692.0 0 52 246 41 692.0 0 41 247 75 692.0 0 75 248 68 692.0 0 68 249 49 692.0 0 49 250 93 692.0 0 93 251 187 692.0 0 187 252 60 692.0 0 60 253 120 692.0 0 120 254 129 692.0 0 129 255 201 692.0 0 201 256 62 692.0 0 62 257 81 692.0 0 81 258 153 692.0 0 153 259 162 692.0 0 162 260 203 692.0 0 203 261 70 692.0 0 70 262 118 692.0 0 118 263 174 692.0 0 174 264 561 692.0 0 561 265 573 692.0 0 573 266 263 692.0 0 263 267 62 692.0 0 62 268 68 692.0 0 68 269 81 692.0 0 81 270 71 692.0 0 71 271 52 692.0 0 52 272 71 692.0 0 71 273 70 692.0 0 70 274 91 692.0 0 91 275 121 692.0 0 121 276 651 692.0 0 651 277 395 692.0 0 395 278 474 692.0 0 474 279 40 692.0 0 40 280 34 692.0 0 34 281 40 692.0 0 40 282 48 692.0 0 48 283 50 692.0 0 50 284 46 692.0 0 46 285 45 692.0 0 45 286 41 692.0 0 41 287 48 692.0 0 48 288 53 692.0 0 53 289 50 692.0 0 50 290 71 692.0 0 71 291 57 692.0 0 57 292 75 692.0 0 75 293 76 692.0 0 76 294 99 692.0 0 99 295 60 692.0 0 60 296 97 692.0 0 97 297 99 692.0 0 99 298 164 692.0 0 164 299 128 692.0 0 128 300 122 692.0 0 122 301 183 692.0 0 183 Finished in 4.17 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 84,523 Reads with adapters: 26,448 (31.3%) Reads that were too short: 59 (0.1%) Reads written (passing filters): 26,389 (31.2%) Total basepairs processed: 21,480,830 bp Total written (filtered): 7,245,400 bp (33.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26448 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 125 0.0 3 0 123 2 17 26094 0.0 3 25560 502 22 10 18 55 0.0 3 6 46 1 2 19 15 0.0 3 4 1 8 2 20 26 0.0 3 0 0 0 26 22 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 36 13 0.0 3 0 0 0 13 37 2 0.0 3 0 0 0 2 39 7 0.0 3 0 0 0 7 42 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 14 0.0 3 0 0 0 14 58 1 0.0 3 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 73 3 0.0 3 0 0 0 3 75 7 0.0 3 0 0 0 7 78 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 2 0.0 3 2 89 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 7 0.0 3 0 0 0 7 93 5 0.0 3 0 0 1 4 99 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 107 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 9 0.0 3 0 0 0 9 161 1 0.0 3 0 0 0 1 179 1 0.0 3 1 192 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.92 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 26,389 Reads with adapters: 549 (2.1%) Reads written (passing filters): 26,389 (100.0%) Total basepairs processed: 7,245,400 bp Total written (filtered): 7,243,737 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 549 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.3% C: 16.8% G: 10.6% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 536 412.3 0 536 4 12 103.1 0 12 7 1 1.6 0 1