This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.86 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 369,174 (13.0%) Reads that were too short: 23,815 (0.8%) Reads written (passing filters): 345,359 (12.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 97,095,867 bp (11.4%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 369174 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1994 692.0 0 1994 7 57957 692.0 0 57957 8 2462 692.0 0 2462 9 45028 692.0 0 45028 10 1928 692.0 0 1928 11 2134 692.0 0 2134 12 38584 692.0 0 38584 13 44478 692.0 0 44478 14 126572 692.0 0 126572 15 220 692.0 0 220 16 42 692.0 0 42 17 35 692.0 0 35 18 34 692.0 0 34 19 33 692.0 0 33 20 21 692.0 0 21 21 29 692.0 0 29 22 26 692.0 0 26 23 23 692.0 0 23 24 37 692.0 0 37 25 29 692.0 0 29 26 72 692.0 0 72 27 120 692.0 0 120 28 51 692.0 0 51 29 38 692.0 0 38 30 31 692.0 0 31 31 30 692.0 0 30 32 34 692.0 0 34 33 40 692.0 0 40 34 38 692.0 0 38 35 24 692.0 0 24 36 32 692.0 0 32 37 16 692.0 0 16 38 35 692.0 0 35 39 35 692.0 0 35 40 43 692.0 0 43 41 32 692.0 0 32 42 27 692.0 0 27 43 84 692.0 0 84 44 42 692.0 0 42 45 51 692.0 0 51 46 34 692.0 0 34 47 23 692.0 0 23 48 25 692.0 0 25 49 37 692.0 0 37 50 35 692.0 0 35 51 38 692.0 0 38 52 30 692.0 0 30 53 32 692.0 0 32 54 21 692.0 0 21 55 26 692.0 0 26 56 34 692.0 0 34 57 30 692.0 0 30 58 47 692.0 0 47 59 28 692.0 0 28 60 23 692.0 0 23 61 22 692.0 0 22 62 23 692.0 0 23 63 38 692.0 0 38 64 31 692.0 0 31 65 46 692.0 0 46 66 36 692.0 0 36 67 44 692.0 0 44 68 27 692.0 0 27 69 17 692.0 0 17 70 43 692.0 0 43 71 31 692.0 0 31 72 38 692.0 0 38 73 91 692.0 0 91 74 50 692.0 0 50 75 35 692.0 0 35 76 41 692.0 0 41 77 31 692.0 0 31 78 50 692.0 0 50 79 55 692.0 0 55 80 37 692.0 0 37 81 38 692.0 0 38 82 36 692.0 0 36 83 41 692.0 0 41 84 47 692.0 0 47 85 39 692.0 0 39 86 27 692.0 0 27 87 46 692.0 0 46 88 39 692.0 0 39 89 45 692.0 0 45 90 55 692.0 0 55 91 41 692.0 0 41 92 104 692.0 0 104 93 44 692.0 0 44 94 68 692.0 0 68 95 66 692.0 0 66 96 61 692.0 0 61 97 43 692.0 0 43 98 65 692.0 0 65 99 46 692.0 0 46 100 39 692.0 0 39 101 35 692.0 0 35 102 57 692.0 0 57 103 33 692.0 0 33 104 211 692.0 0 211 105 155 692.0 0 155 106 523 692.0 0 523 107 98 692.0 0 98 108 179 692.0 0 179 109 86 692.0 0 86 110 139 692.0 0 139 111 104 692.0 0 104 112 112 692.0 0 112 113 95 692.0 0 95 114 94 692.0 0 94 115 70 692.0 0 70 116 67 692.0 0 67 117 63 692.0 0 63 118 63 692.0 0 63 119 83 692.0 0 83 120 75 692.0 0 75 121 76 692.0 0 76 122 75 692.0 0 75 123 111 692.0 0 111 124 1031 692.0 0 1031 125 764 692.0 0 764 126 3085 692.0 0 3085 127 450 692.0 0 450 128 461 692.0 0 461 129 204 692.0 0 204 130 64 692.0 0 64 131 76 692.0 0 76 132 43 692.0 0 43 133 72 692.0 0 72 134 80 692.0 0 80 135 127 692.0 0 127 136 87 692.0 0 87 137 116 692.0 0 116 138 708 692.0 0 708 139 335 692.0 0 335 140 1245 692.0 0 1245 141 140 692.0 0 140 142 231 692.0 0 231 143 63 692.0 0 63 144 67 692.0 0 67 145 102 692.0 0 102 146 158 692.0 0 158 147 107 692.0 0 107 148 166 692.0 0 166 149 53 692.0 0 53 150 77 692.0 0 77 151 407 692.0 0 407 152 137 692.0 0 137 153 251 692.0 0 251 154 77 692.0 0 77 155 83 692.0 0 83 156 90 692.0 0 90 157 150 692.0 0 150 158 756 692.0 0 756 159 444 692.0 0 444 160 538 692.0 0 538 161 1280 692.0 0 1280 162 140 692.0 0 140 163 169 692.0 0 169 164 58 692.0 0 58 165 71 692.0 0 71 166 50 692.0 0 50 167 86 692.0 0 86 168 66 692.0 0 66 169 118 692.0 0 118 170 46 692.0 0 46 171 61 692.0 0 61 172 44 692.0 0 44 173 73 692.0 0 73 174 100 692.0 0 100 175 83 692.0 0 83 176 76 692.0 0 76 177 82 692.0 0 82 178 42 692.0 0 42 179 79 692.0 0 79 180 62 692.0 0 62 181 114 692.0 0 114 182 142 692.0 0 142 183 158 692.0 0 158 184 82 692.0 0 82 185 65 692.0 0 65 186 79 692.0 0 79 187 62 692.0 0 62 188 253 692.0 0 253 189 188 692.0 0 188 190 210 692.0 0 210 191 45 692.0 0 45 192 58 692.0 0 58 193 59 692.0 0 59 194 69 692.0 0 69 195 65 692.0 0 65 196 56 692.0 0 56 197 69 692.0 0 69 198 80 692.0 0 80 199 108 692.0 0 108 200 77 692.0 0 77 201 105 692.0 0 105 202 87 692.0 0 87 203 70 692.0 0 70 204 57 692.0 0 57 205 58 692.0 0 58 206 57 692.0 0 57 207 114 692.0 0 114 208 81 692.0 0 81 209 125 692.0 0 125 210 70 692.0 0 70 211 68 692.0 0 68 212 72 692.0 0 72 213 103 692.0 0 103 214 234 692.0 0 234 215 144 692.0 0 144 216 133 692.0 0 133 217 83 692.0 0 83 218 94 692.0 0 94 219 101 692.0 0 101 220 353 692.0 0 353 221 451 692.0 0 451 222 216 692.0 0 216 223 106 692.0 0 106 224 370 692.0 0 370 225 84 692.0 0 84 226 465 692.0 0 465 227 73 692.0 0 73 228 56 692.0 0 56 229 53 692.0 0 53 230 113 692.0 0 113 231 172 692.0 0 172 232 198 692.0 0 198 233 478 692.0 0 478 234 317 692.0 0 317 235 137 692.0 0 137 236 102 692.0 0 102 237 64 692.0 0 64 238 57 692.0 0 57 239 47 692.0 0 47 240 90 692.0 0 90 241 98 692.0 0 98 242 158 692.0 0 158 243 105 692.0 0 105 244 107 692.0 0 107 245 73 692.0 0 73 246 52 692.0 0 52 247 65 692.0 0 65 248 58 692.0 0 58 249 57 692.0 0 57 250 65 692.0 0 65 251 108 692.0 0 108 252 90 692.0 0 90 253 145 692.0 0 145 254 115 692.0 0 115 255 65 692.0 0 65 256 68 692.0 0 68 257 92 692.0 0 92 258 89 692.0 0 89 259 57 692.0 0 57 260 90 692.0 0 90 261 112 692.0 0 112 262 123 692.0 0 123 263 128 692.0 0 128 264 93 692.0 0 93 265 86 692.0 0 86 266 104 692.0 0 104 267 158 692.0 0 158 268 192 692.0 0 192 269 359 692.0 0 359 270 135 692.0 0 135 271 116 692.0 0 116 272 74 692.0 0 74 273 67 692.0 0 67 274 103 692.0 0 103 275 102 692.0 0 102 276 166 692.0 0 166 277 630 692.0 0 630 278 334 692.0 0 334 279 396 692.0 0 396 280 947 692.0 0 947 281 4039 692.0 0 4039 282 2554 692.0 0 2554 283 2433 692.0 0 2433 284 539 692.0 0 539 285 102 692.0 0 102 286 80 692.0 0 80 287 93 692.0 0 93 288 206 692.0 0 206 289 231 692.0 0 231 290 536 692.0 0 536 291 88 692.0 0 88 292 117 692.0 0 117 293 77 692.0 0 77 294 122 692.0 0 122 295 131 692.0 0 131 296 176 692.0 0 176 297 89 692.0 0 89 298 69 692.0 0 69 299 49 692.0 0 49 300 63 692.0 0 63 301 86 692.0 0 86 Finished in 17.80 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 345,359 Reads with adapters: 47,265 (13.7%) Reads that were too short: 236 (0.1%) Reads written (passing filters): 47,029 (13.6%) Total basepairs processed: 97,095,867 bp Total written (filtered): 12,709,394 bp (13.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 47265 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 3 2 18 17 0.0 3 0 0 2 11 4 19 234 0.0 3 0 139 32 37 26 20 45601 0.0 4 42517 2362 516 148 58 21 104 0.0 4 12 80 10 1 1 22 21 0.0 4 1 1 11 3 5 23 258 0.0 4 0 0 0 194 64 36 2 0.0 4 0 0 0 0 2 42 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 81 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 114 2 0.0 4 0 0 0 0 2 117 2 0.0 4 0 0 0 0 2 120 3 0.0 4 0 0 0 0 3 121 9 0.0 4 0 0 0 0 9 122 635 0.0 4 0 0 0 0 635 123 5 0.0 4 0 0 0 0 5 124 1 0.0 4 0 0 0 0 1 125 6 0.0 4 0 0 0 0 6 127 88 0.0 4 0 0 0 0 88 130 4 0.0 4 1 0 0 0 3 131 2 0.0 4 0 0 0 1 1 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 144 2 0.0 4 0 0 0 0 2 147 2 0.0 4 0 0 0 0 2 148 11 0.0 4 0 0 0 0 11 149 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 1 177 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 198 2 0.0 4 0 0 0 0 2 202 2 0.0 4 0 0 0 0 2 203 3 0.0 4 0 0 0 0 3 204 2 0.0 4 0 0 0 0 2 206 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 209 3 0.0 4 0 0 0 0 3 210 4 0.0 4 0 0 0 0 4 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 216 5 0.0 4 0 0 0 0 5 217 3 0.0 4 0 0 0 0 3 219 1 0.0 4 0 0 0 0 1 220 2 0.0 4 0 0 0 0 2 221 3 0.0 4 0 0 0 0 3 222 2 0.0 4 0 0 0 0 2 223 19 0.0 4 0 0 0 1 18 224 10 0.0 4 0 0 0 0 10 225 1 0.0 4 0 0 0 0 1 227 6 0.0 4 0 0 0 1 5 228 7 0.0 4 0 0 0 0 7 229 8 0.0 4 0 0 0 1 7 230 7 0.0 4 0 0 0 1 6 231 11 0.0 4 0 0 0 2 9 232 19 0.0 4 0 0 0 1 18 235 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 252 3 0.0 4 0 0 0 0 3 253 2 0.0 4 0 0 0 0 2 257 3 0.0 4 0 0 0 0 3 258 3 0.0 4 0 0 0 0 3 259 4 0.0 4 0 0 0 0 4 260 5 0.0 4 0 0 0 0 5 263 4 0.0 4 0 0 0 1 3 264 3 0.0 4 0 0 0 0 3 265 2 0.0 4 0 0 0 0 2 267 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 274 2 0.0 4 0 0 0 0 2 275 2 0.0 4 0 0 0 0 2 276 3 0.0 4 0 0 0 1 2 277 7 0.0 4 0 0 0 0 7 278 4 0.0 4 0 0 0 0 4 279 3 0.0 4 0 0 0 0 3 280 2 0.0 4 0 0 0 1 1 282 3 0.0 4 0 0 0 0 3 283 7 0.0 4 0 0 0 0 7 284 1 0.0 4 0 0 0 0 1 285 4 0.0 4 0 0 0 0 4 286 2 0.0 4 0 0 0 0 2 287 8 0.0 4 0 0 0 0 8 288 1 0.0 4 0 0 0 0 1 289 4 0.0 4 0 0 0 0 4 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.76 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 47,029 Reads with adapters: 11,531 (24.5%) Reads written (passing filters): 47,029 (100.0%) Total basepairs processed: 12,709,394 bp Total written (filtered): 12,591,173 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11531 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 9.9% G: 85.1% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 734.8 0 17 4 6 183.7 0 6 8 5 0.7 0 5 9 1992 0.2 0 219 1773 10 8524 0.0 1 785 7739 11 891 0.0 1 41 850 12 37 0.0 1 4 33 13 31 0.0 1 0 31 14 1 0.0 1 0 1 72 2 0.0 1 1 1 80 1 0.0 1 1 150 12 0.0 1 9 3 151 2 0.0 1 2 152 3 0.0 1 2 1 208 5 0.0 1 5 226 1 0.0 1 1 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.