This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.53 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 343,279 (12.1%) Reads that were too short: 13,618 (0.5%) Reads written (passing filters): 329,661 (11.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 93,755,552 bp (11.0%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 343279 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1819 692.0 0 1819 7 47941 692.0 0 47941 8 1338 692.0 0 1338 9 59100 692.0 0 59100 10 1498 692.0 0 1498 11 1492 692.0 0 1492 12 36176 692.0 0 36176 13 42799 692.0 0 42799 14 123094 692.0 0 123094 15 138 692.0 0 138 16 29 692.0 0 29 17 14 692.0 0 14 18 19 692.0 0 19 19 15 692.0 0 15 20 18 692.0 0 18 21 16 692.0 0 16 22 26 692.0 0 26 23 16 692.0 0 16 24 15 692.0 0 15 25 20 692.0 0 20 26 19 692.0 0 19 27 17 692.0 0 17 28 18 692.0 0 18 29 18 692.0 0 18 30 22 692.0 0 22 31 21 692.0 0 21 32 15 692.0 0 15 33 19 692.0 0 19 34 15 692.0 0 15 35 17 692.0 0 17 36 192 692.0 0 192 37 23 692.0 0 23 38 37 692.0 0 37 39 17 692.0 0 17 40 35 692.0 0 35 41 26 692.0 0 26 42 25 692.0 0 25 43 59 692.0 0 59 44 38 692.0 0 38 45 38 692.0 0 38 46 15 692.0 0 15 47 18 692.0 0 18 48 19 692.0 0 19 49 30 692.0 0 30 50 22 692.0 0 22 51 32 692.0 0 32 52 25 692.0 0 25 53 29 692.0 0 29 54 15 692.0 0 15 55 19 692.0 0 19 56 20 692.0 0 20 57 25 692.0 0 25 58 22 692.0 0 22 59 25 692.0 0 25 60 16 692.0 0 16 61 31 692.0 0 31 62 15 692.0 0 15 63 29 692.0 0 29 64 32 692.0 0 32 65 36 692.0 0 36 66 29 692.0 0 29 67 24 692.0 0 24 68 28 692.0 0 28 69 19 692.0 0 19 70 27 692.0 0 27 71 29 692.0 0 29 72 12 692.0 0 12 73 58 692.0 0 58 74 34 692.0 0 34 75 27 692.0 0 27 76 30 692.0 0 30 77 30 692.0 0 30 78 48 692.0 0 48 79 44 692.0 0 44 80 42 692.0 0 42 81 36 692.0 0 36 82 34 692.0 0 34 83 28 692.0 0 28 84 42 692.0 0 42 85 43 692.0 0 43 86 37 692.0 0 37 87 55 692.0 0 55 88 44 692.0 0 44 89 61 692.0 0 61 90 53 692.0 0 53 91 60 692.0 0 60 92 92 692.0 0 92 93 43 692.0 0 43 94 58 692.0 0 58 95 70 692.0 0 70 96 52 692.0 0 52 97 48 692.0 0 48 98 56 692.0 0 56 99 28 692.0 0 28 100 34 692.0 0 34 101 36 692.0 0 36 102 35 692.0 0 35 103 44 692.0 0 44 104 160 692.0 0 160 105 131 692.0 0 131 106 315 692.0 0 315 107 52 692.0 0 52 108 105 692.0 0 105 109 60 692.0 0 60 110 78 692.0 0 78 111 56 692.0 0 56 112 62 692.0 0 62 113 55 692.0 0 55 114 52 692.0 0 52 115 41 692.0 0 41 116 53 692.0 0 53 117 46 692.0 0 46 118 44 692.0 0 44 119 41 692.0 0 41 120 75 692.0 0 75 121 95 692.0 0 95 122 74 692.0 0 74 123 47 692.0 0 47 124 392 692.0 0 392 125 313 692.0 0 313 126 1281 692.0 0 1281 127 413 692.0 0 413 128 408 692.0 0 408 129 174 692.0 0 174 130 55 692.0 0 55 131 43 692.0 0 43 132 52 692.0 0 52 133 52 692.0 0 52 134 60 692.0 0 60 135 105 692.0 0 105 136 74 692.0 0 74 137 62 692.0 0 62 138 172 692.0 0 172 139 191 692.0 0 191 140 295 692.0 0 295 141 100 692.0 0 100 142 158 692.0 0 158 143 45 692.0 0 45 144 44 692.0 0 44 145 77 692.0 0 77 146 96 692.0 0 96 147 61 692.0 0 61 148 85 692.0 0 85 149 40 692.0 0 40 150 64 692.0 0 64 151 285 692.0 0 285 152 108 692.0 0 108 153 144 692.0 0 144 154 58 692.0 0 58 155 68 692.0 0 68 156 70 692.0 0 70 157 103 692.0 0 103 158 337 692.0 0 337 159 292 692.0 0 292 160 285 692.0 0 285 161 639 692.0 0 639 162 78 692.0 0 78 163 83 692.0 0 83 164 66 692.0 0 66 165 52 692.0 0 52 166 51 692.0 0 51 167 56 692.0 0 56 168 41 692.0 0 41 169 89 692.0 0 89 170 33 692.0 0 33 171 43 692.0 0 43 172 51 692.0 0 51 173 46 692.0 0 46 174 73 692.0 0 73 175 67 692.0 0 67 176 77 692.0 0 77 177 43 692.0 0 43 178 23 692.0 0 23 179 50 692.0 0 50 180 52 692.0 0 52 181 67 692.0 0 67 182 77 692.0 0 77 183 55 692.0 0 55 184 51 692.0 0 51 185 42 692.0 0 42 186 52 692.0 0 52 187 62 692.0 0 62 188 142 692.0 0 142 189 119 692.0 0 119 190 99 692.0 0 99 191 39 692.0 0 39 192 52 692.0 0 52 193 59 692.0 0 59 194 54 692.0 0 54 195 42 692.0 0 42 196 30 692.0 0 30 197 60 692.0 0 60 198 53 692.0 0 53 199 75 692.0 0 75 200 69 692.0 0 69 201 46 692.0 0 46 202 72 692.0 0 72 203 63 692.0 0 63 204 49 692.0 0 49 205 41 692.0 0 41 206 46 692.0 0 46 207 74 692.0 0 74 208 75 692.0 0 75 209 66 692.0 0 66 210 33 692.0 0 33 211 44 692.0 0 44 212 55 692.0 0 55 213 59 692.0 0 59 214 153 692.0 0 153 215 87 692.0 0 87 216 128 692.0 0 128 217 39 692.0 0 39 218 58 692.0 0 58 219 58 692.0 0 58 220 195 692.0 0 195 221 286 692.0 0 286 222 176 692.0 0 176 223 77 692.0 0 77 224 412 692.0 0 412 225 88 692.0 0 88 226 465 692.0 0 465 227 44 692.0 0 44 228 47 692.0 0 47 229 49 692.0 0 49 230 77 692.0 0 77 231 88 692.0 0 88 232 90 692.0 0 90 233 225 692.0 0 225 234 241 692.0 0 241 235 135 692.0 0 135 236 61 692.0 0 61 237 64 692.0 0 64 238 51 692.0 0 51 239 44 692.0 0 44 240 71 692.0 0 71 241 87 692.0 0 87 242 125 692.0 0 125 243 70 692.0 0 70 244 55 692.0 0 55 245 45 692.0 0 45 246 45 692.0 0 45 247 60 692.0 0 60 248 46 692.0 0 46 249 42 692.0 0 42 250 62 692.0 0 62 251 77 692.0 0 77 252 75 692.0 0 75 253 81 692.0 0 81 254 59 692.0 0 59 255 39 692.0 0 39 256 58 692.0 0 58 257 49 692.0 0 49 258 62 692.0 0 62 259 39 692.0 0 39 260 77 692.0 0 77 261 89 692.0 0 89 262 98 692.0 0 98 263 129 692.0 0 129 264 85 692.0 0 85 265 50 692.0 0 50 266 87 692.0 0 87 267 131 692.0 0 131 268 103 692.0 0 103 269 258 692.0 0 258 270 88 692.0 0 88 271 76 692.0 0 76 272 83 692.0 0 83 273 59 692.0 0 59 274 73 692.0 0 73 275 88 692.0 0 88 276 137 692.0 0 137 277 683 692.0 0 683 278 328 692.0 0 328 279 356 692.0 0 356 280 317 692.0 0 317 281 1307 692.0 0 1307 282 766 692.0 0 766 283 831 692.0 0 831 284 193 692.0 0 193 285 51 692.0 0 51 286 65 692.0 0 65 287 55 692.0 0 55 288 171 692.0 0 171 289 171 692.0 0 171 290 380 692.0 0 380 291 84 692.0 0 84 292 87 692.0 0 87 293 65 692.0 0 65 294 99 692.0 0 99 295 111 692.0 0 111 296 105 692.0 0 105 297 68 692.0 0 68 298 42 692.0 0 42 299 54 692.0 0 54 300 45 692.0 0 45 301 111 692.0 0 111 Finished in 14.96 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 329,661 Reads with adapters: 49,502 (15.0%) Reads that were too short: 273 (0.1%) Reads written (passing filters): 49,229 (14.9%) Total basepairs processed: 93,755,552 bp Total written (filtered): 13,264,808 bp (14.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49502 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 150 0.0 3 0 149 1 17 46587 0.0 3 45721 844 20 2 18 85 0.0 3 1 83 0 1 19 25 0.0 3 0 0 20 5 20 45 0.0 3 28 2 0 15 23 3 0.0 3 1 0 0 2 25 1 0.0 3 1 27 2 0.0 3 2 28 1 0.0 3 0 0 0 1 32 19 0.0 3 0 0 0 19 35 3 0.0 3 0 0 0 3 37 1 0.0 3 1 41 1 0.0 3 0 0 0 1 43 7 0.0 3 0 0 3 4 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 53 13 0.0 3 0 0 0 13 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 65 1 0.0 3 1 67 42 0.0 3 0 0 1 41 70 8 0.0 3 0 0 0 8 71 3 0.0 3 0 0 3 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 6 0.0 3 0 0 1 5 89 8 0.0 3 1 0 0 7 91 1 0.0 3 0 0 0 1 99 1 0.0 3 1 101 7 0.0 3 0 0 7 102 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 43 0.0 3 0 0 0 43 111 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 29 0.0 3 0 0 1 28 125 2 0.0 3 0 0 0 2 128 3 0.0 3 1 1 1 129 2 0.0 3 0 1 0 1 130 1 0.0 3 0 0 1 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 154 30 0.0 3 0 0 0 30 155 5 0.0 3 0 0 0 5 156 2 0.0 3 0 0 0 2 157 10 0.0 3 0 0 0 10 158 4 0.0 3 0 0 0 4 159 232 0.0 3 0 0 1 231 160 105 0.0 3 0 0 1 104 161 48 0.0 3 0 0 0 48 162 52 0.0 3 0 0 0 52 163 22 0.0 3 0 0 0 22 164 13 0.0 3 0 0 0 13 165 49 0.0 3 0 0 1 48 166 2 0.0 3 0 0 0 2 167 13 0.0 3 0 0 0 13 168 17 0.0 3 0 0 0 17 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 19 0.0 3 0 0 0 19 174 15 0.0 3 0 0 0 15 175 12 0.0 3 0 0 0 12 176 13 0.0 3 0 0 0 13 177 8 0.0 3 0 0 1 7 178 623 0.0 3 0 0 7 616 179 345 0.0 3 0 0 0 345 180 396 0.0 3 0 0 0 396 181 3 0.0 3 0 0 1 2 182 38 0.0 3 0 0 2 36 183 21 0.0 3 0 0 2 19 184 1 0.0 3 0 0 0 1 185 6 0.0 3 0 0 1 5 186 2 0.0 3 0 0 0 2 188 4 0.0 3 0 0 0 4 189 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 206 4 0.0 3 0 0 0 4 210 3 0.0 3 0 0 0 3 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 13 0.0 3 0 0 0 13 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 1 238 1 0.0 3 0 0 0 1 239 7 0.0 3 0 0 0 7 240 6 0.0 3 0 0 0 6 242 2 0.0 3 0 0 0 2 252 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 0 2 264 3 0.0 3 0 0 0 3 271 15 0.0 3 0 0 0 15 272 16 0.0 3 0 0 1 15 273 9 0.0 3 0 0 0 9 274 7 0.0 3 0 0 0 7 275 36 0.0 3 0 0 1 35 276 5 0.0 3 0 0 1 4 277 2 0.0 3 0 0 0 2 279 8 0.0 3 0 0 0 8 280 22 0.0 3 0 0 0 22 281 21 0.0 3 0 0 0 21 284 2 0.0 3 0 0 0 2 285 4 0.0 3 0 0 0 4 286 3 0.0 3 0 0 0 3 287 9 0.0 3 0 0 0 9 288 26 0.0 3 0 0 0 26 289 5 0.0 3 0 0 0 5 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.69 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 49,229 Reads with adapters: 270 (0.5%) Reads written (passing filters): 49,229 (100.0%) Total basepairs processed: 13,264,808 bp Total written (filtered): 13,263,975 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 270 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.8% C: 50.7% G: 9.6% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 252 769.2 0 252 4 14 192.3 0 14 5 3 48.1 0 3 6 1 12.0 0 1