This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.55 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 38,577 (1.4%) Reads that were too short: 10,108 (0.4%) Reads written (passing filters): 28,469 (1.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 6,992,463 bp (0.8%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 38577 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1572 692.0 0 1572 7 8732 692.0 0 8732 8 75 692.0 0 75 9 6622 692.0 0 6622 10 26 692.0 0 26 11 41 692.0 0 41 12 8 692.0 0 8 13 20 692.0 0 20 14 20 692.0 0 20 15 18 692.0 0 18 16 22 692.0 0 22 17 23 692.0 0 23 18 6 692.0 0 6 19 21 692.0 0 21 20 27 692.0 0 27 21 24 692.0 0 24 22 18 692.0 0 18 23 29 692.0 0 29 24 33 692.0 0 33 25 23 692.0 0 23 26 17 692.0 0 17 27 28 692.0 0 28 28 23 692.0 0 23 29 22 692.0 0 22 30 17 692.0 0 17 31 23 692.0 0 23 32 27 692.0 0 27 33 20 692.0 0 20 34 21 692.0 0 21 35 22 692.0 0 22 36 13 692.0 0 13 37 11 692.0 0 11 38 21 692.0 0 21 39 18 692.0 0 18 40 31 692.0 0 31 41 22 692.0 0 22 42 15 692.0 0 15 43 50 692.0 0 50 44 18 692.0 0 18 45 17 692.0 0 17 46 15 692.0 0 15 47 18 692.0 0 18 48 22 692.0 0 22 49 24 692.0 0 24 50 109 692.0 0 109 51 23 692.0 0 23 52 25 692.0 0 25 53 38 692.0 0 38 54 25 692.0 0 25 55 33 692.0 0 33 56 25 692.0 0 25 57 21 692.0 0 21 58 31 692.0 0 31 59 31 692.0 0 31 60 29 692.0 0 29 61 18 692.0 0 18 62 16 692.0 0 16 63 15 692.0 0 15 64 25 692.0 0 25 65 29 692.0 0 29 66 21 692.0 0 21 67 33 692.0 0 33 68 36 692.0 0 36 69 39 692.0 0 39 70 32 692.0 0 32 71 130 692.0 0 130 72 26 692.0 0 26 73 80 692.0 0 80 74 68 692.0 0 68 75 67 692.0 0 67 76 12 692.0 0 12 77 16 692.0 0 16 78 14 692.0 0 14 79 31 692.0 0 31 80 50 692.0 0 50 81 35 692.0 0 35 82 33 692.0 0 33 83 34 692.0 0 34 84 36 692.0 0 36 85 32 692.0 0 32 86 48 692.0 0 48 87 33 692.0 0 33 88 34 692.0 0 34 89 24 692.0 0 24 90 42 692.0 0 42 91 46 692.0 0 46 92 30 692.0 0 30 93 34 692.0 0 34 94 20 692.0 0 20 95 28 692.0 0 28 96 35 692.0 0 35 97 29 692.0 0 29 98 27 692.0 0 27 99 34 692.0 0 34 100 54 692.0 0 54 101 449 692.0 0 449 102 157 692.0 0 157 103 218 692.0 0 218 104 51 692.0 0 51 105 57 692.0 0 57 106 42 692.0 0 42 107 31 692.0 0 31 108 36 692.0 0 36 109 25 692.0 0 25 110 31 692.0 0 31 111 167 692.0 0 167 112 122 692.0 0 122 113 92 692.0 0 92 114 62 692.0 0 62 115 91 692.0 0 91 116 45 692.0 0 45 117 60 692.0 0 60 118 49 692.0 0 49 119 27 692.0 0 27 120 34 692.0 0 34 121 40 692.0 0 40 122 36 692.0 0 36 123 87 692.0 0 87 124 90 692.0 0 90 125 74 692.0 0 74 126 45 692.0 0 45 127 36 692.0 0 36 128 28 692.0 0 28 129 36 692.0 0 36 130 79 692.0 0 79 131 82 692.0 0 82 132 357 692.0 0 357 133 184 692.0 0 184 134 102 692.0 0 102 135 31 692.0 0 31 136 55 692.0 0 55 137 78 692.0 0 78 138 74 692.0 0 74 139 130 692.0 0 130 140 55 692.0 0 55 141 143 692.0 0 143 142 91 692.0 0 91 143 113 692.0 0 113 144 236 692.0 0 236 145 221 692.0 0 221 146 347 692.0 0 347 147 42 692.0 0 42 148 77 692.0 0 77 149 115 692.0 0 115 150 91 692.0 0 91 151 72 692.0 0 72 152 36 692.0 0 36 153 37 692.0 0 37 154 100 692.0 0 100 155 81 692.0 0 81 156 97 692.0 0 97 157 47 692.0 0 47 158 252 692.0 0 252 159 51 692.0 0 51 160 49 692.0 0 49 161 40 692.0 0 40 162 50 692.0 0 50 163 50 692.0 0 50 164 54 692.0 0 54 165 41 692.0 0 41 166 76 692.0 0 76 167 36 692.0 0 36 168 64 692.0 0 64 169 106 692.0 0 106 170 534 692.0 0 534 171 162 692.0 0 162 172 280 692.0 0 280 173 33 692.0 0 33 174 15 692.0 0 15 175 38 692.0 0 38 176 40 692.0 0 40 177 65 692.0 0 65 178 105 692.0 0 105 179 75 692.0 0 75 180 76 692.0 0 76 181 36 692.0 0 36 182 32 692.0 0 32 183 46 692.0 0 46 184 48 692.0 0 48 185 53 692.0 0 53 186 39 692.0 0 39 187 46 692.0 0 46 188 46 692.0 0 46 189 61 692.0 0 61 190 40 692.0 0 40 191 26 692.0 0 26 192 24 692.0 0 24 193 29 692.0 0 29 194 35 692.0 0 35 195 55 692.0 0 55 196 55 692.0 0 55 197 56 692.0 0 56 198 69 692.0 0 69 199 90 692.0 0 90 200 49 692.0 0 49 201 40 692.0 0 40 202 42 692.0 0 42 203 61 692.0 0 61 204 70 692.0 0 70 205 98 692.0 0 98 206 67 692.0 0 67 207 39 692.0 0 39 208 39 692.0 0 39 209 66 692.0 0 66 210 102 692.0 0 102 211 83 692.0 0 83 212 226 692.0 0 226 213 67 692.0 0 67 214 48 692.0 0 48 215 61 692.0 0 61 216 53 692.0 0 53 217 50 692.0 0 50 218 66 692.0 0 66 219 43 692.0 0 43 220 42 692.0 0 42 221 35 692.0 0 35 222 50 692.0 0 50 223 75 692.0 0 75 224 104 692.0 0 104 225 123 692.0 0 123 226 218 692.0 0 218 227 113 692.0 0 113 228 99 692.0 0 99 229 104 692.0 0 104 230 29 692.0 0 29 231 29 692.0 0 29 232 43 692.0 0 43 233 53 692.0 0 53 234 55 692.0 0 55 235 47 692.0 0 47 236 60 692.0 0 60 237 74 692.0 0 74 238 64 692.0 0 64 239 63 692.0 0 63 240 43 692.0 0 43 241 46 692.0 0 46 242 45 692.0 0 45 243 60 692.0 0 60 244 112 692.0 0 112 245 163 692.0 0 163 246 125 692.0 0 125 247 184 692.0 0 184 248 85 692.0 0 85 249 78 692.0 0 78 250 46 692.0 0 46 251 36 692.0 0 36 252 51 692.0 0 51 253 59 692.0 0 59 254 53 692.0 0 53 255 69 692.0 0 69 256 81 692.0 0 81 257 105 692.0 0 105 258 108 692.0 0 108 259 93 692.0 0 93 260 73 692.0 0 73 261 87 692.0 0 87 262 64 692.0 0 64 263 109 692.0 0 109 264 268 692.0 0 268 265 156 692.0 0 156 266 178 692.0 0 178 267 54 692.0 0 54 268 59 692.0 0 59 269 36 692.0 0 36 270 62 692.0 0 62 271 91 692.0 0 91 272 132 692.0 0 132 273 123 692.0 0 123 274 83 692.0 0 83 275 75 692.0 0 75 276 60 692.0 0 60 277 51 692.0 0 51 278 78 692.0 0 78 279 75 692.0 0 75 280 74 692.0 0 74 281 84 692.0 0 84 282 128 692.0 0 128 283 153 692.0 0 153 284 719 692.0 0 719 285 606 692.0 0 606 286 564 692.0 0 564 287 248 692.0 0 248 288 59 692.0 0 59 289 60 692.0 0 60 290 50 692.0 0 50 291 100 692.0 0 100 292 88 692.0 0 88 293 189 692.0 0 189 294 76 692.0 0 76 295 80 692.0 0 80 296 58 692.0 0 58 297 76 692.0 0 76 298 108 692.0 0 108 299 100 692.0 0 100 300 92 692.0 0 92 301 79 692.0 0 79 Finished in 1.33 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 28,469 Reads with adapters: 6,736 (23.7%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 6,730 (23.6%) Total basepairs processed: 6,992,463 bp Total written (filtered): 1,849,170 bp (26.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6736 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 32 0.0 3 0 32 17 6647 0.0 3 6509 132 6 18 11 0.0 3 1 10 19 8 0.0 3 5 0 3 20 20 0.0 3 2 0 0 18 47 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 6,730 Reads with adapters: 174 (2.6%) Reads written (passing filters): 6,730 (100.0%) Total basepairs processed: 1,849,170 bp Total written (filtered): 1,848,641 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 174 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.0% C: 5.2% G: 36.8% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 167 105.2 0 167 4 7 26.3 0 7