This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.24 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 266,733 (9.4%) Reads that were too short: 113,699 (4.0%) Reads written (passing filters): 153,034 (5.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 42,662,475 bp (5.0%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 266733 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1121 692.0 0 1121 7 2665 692.0 0 2665 8 66634 692.0 0 66634 9 62045 692.0 0 62045 10 76 692.0 0 76 11 45 692.0 0 45 12 30 692.0 0 30 13 11 692.0 0 11 14 18 692.0 0 18 15 18 692.0 0 18 16 11 692.0 0 11 17 14 692.0 0 14 18 7 692.0 0 7 19 9 692.0 0 9 20 19 692.0 0 19 21 13 692.0 0 13 22 24 692.0 0 24 23 9 692.0 0 9 24 21 692.0 0 21 25 18 692.0 0 18 26 39 692.0 0 39 27 13 692.0 0 13 28 21 692.0 0 21 29 10 692.0 0 10 30 13 692.0 0 13 31 18 692.0 0 18 32 18 692.0 0 18 33 19 692.0 0 19 34 31 692.0 0 31 35 10 692.0 0 10 36 32 692.0 0 32 37 13 692.0 0 13 38 32 692.0 0 32 39 13 692.0 0 13 40 13 692.0 0 13 41 26 692.0 0 26 42 30 692.0 0 30 43 62 692.0 0 62 44 10 692.0 0 10 45 20 692.0 0 20 46 15 692.0 0 15 47 16 692.0 0 16 48 28 692.0 0 28 49 38 692.0 0 38 50 31 692.0 0 31 51 23 692.0 0 23 52 22 692.0 0 22 53 11 692.0 0 11 54 18 692.0 0 18 55 23 692.0 0 23 56 68 692.0 0 68 57 99 692.0 0 99 58 94 692.0 0 94 59 26 692.0 0 26 60 25 692.0 0 25 61 57 692.0 0 57 62 25 692.0 0 25 63 28 692.0 0 28 64 53 692.0 0 53 65 32 692.0 0 32 66 14 692.0 0 14 67 30 692.0 0 30 68 30 692.0 0 30 69 165 692.0 0 165 70 294 692.0 0 294 71 325 692.0 0 325 72 58 692.0 0 58 73 37 692.0 0 37 74 55 692.0 0 55 75 94 692.0 0 94 76 165 692.0 0 165 77 68 692.0 0 68 78 83 692.0 0 83 79 94 692.0 0 94 80 57 692.0 0 57 81 60 692.0 0 60 82 87 692.0 0 87 83 69 692.0 0 69 84 63 692.0 0 63 85 208 692.0 0 208 86 95 692.0 0 95 87 79 692.0 0 79 88 113 692.0 0 113 89 1860 692.0 0 1860 90 893 692.0 0 893 91 6034 692.0 0 6034 92 54 692.0 0 54 93 30 692.0 0 30 94 60 692.0 0 60 95 25 692.0 0 25 96 34 692.0 0 34 97 136 692.0 0 136 98 66 692.0 0 66 99 103 692.0 0 103 100 59 692.0 0 59 101 34 692.0 0 34 102 38 692.0 0 38 103 28 692.0 0 28 104 21 692.0 0 21 105 23 692.0 0 23 106 18 692.0 0 18 107 25 692.0 0 25 108 40 692.0 0 40 109 29 692.0 0 29 110 31 692.0 0 31 111 62 692.0 0 62 112 76 692.0 0 76 113 71 692.0 0 71 114 19 692.0 0 19 115 15 692.0 0 15 116 21 692.0 0 21 117 64 692.0 0 64 118 29 692.0 0 29 119 22 692.0 0 22 120 39 692.0 0 39 121 28 692.0 0 28 122 16 692.0 0 16 123 25 692.0 0 25 124 20 692.0 0 20 125 28 692.0 0 28 126 26 692.0 0 26 127 14 692.0 0 14 128 56 692.0 0 56 129 34 692.0 0 34 130 41 692.0 0 41 131 24 692.0 0 24 132 36 692.0 0 36 133 30 692.0 0 30 134 44 692.0 0 44 135 27 692.0 0 27 136 26 692.0 0 26 137 74 692.0 0 74 138 26 692.0 0 26 139 48 692.0 0 48 140 32 692.0 0 32 141 28 692.0 0 28 142 26 692.0 0 26 143 24 692.0 0 24 144 27 692.0 0 27 145 42 692.0 0 42 146 39 692.0 0 39 147 37 692.0 0 37 148 181 692.0 0 181 149 97 692.0 0 97 150 242 692.0 0 242 151 24 692.0 0 24 152 27 692.0 0 27 153 37 692.0 0 37 154 40 692.0 0 40 155 31 692.0 0 31 156 121 692.0 0 121 157 76 692.0 0 76 158 34 692.0 0 34 159 67 692.0 0 67 160 61 692.0 0 61 161 32 692.0 0 32 162 40 692.0 0 40 163 31 692.0 0 31 164 29 692.0 0 29 165 33 692.0 0 33 166 32 692.0 0 32 167 51 692.0 0 51 168 389 692.0 0 389 169 152 692.0 0 152 170 138 692.0 0 138 171 31 692.0 0 31 172 31 692.0 0 31 173 30 692.0 0 30 174 76 692.0 0 76 175 109 692.0 0 109 176 1663 692.0 0 1663 177 745 692.0 0 745 178 312 692.0 0 312 179 95 692.0 0 95 180 27 692.0 0 27 181 16 692.0 0 16 182 42 692.0 0 42 183 48 692.0 0 48 184 41 692.0 0 41 185 32 692.0 0 32 186 30 692.0 0 30 187 42 692.0 0 42 188 28 692.0 0 28 189 34 692.0 0 34 190 27 692.0 0 27 191 26 692.0 0 26 192 26 692.0 0 26 193 35 692.0 0 35 194 48 692.0 0 48 195 38 692.0 0 38 196 49 692.0 0 49 197 26 692.0 0 26 198 31 692.0 0 31 199 21 692.0 0 21 200 20 692.0 0 20 201 27 692.0 0 27 202 132 692.0 0 132 203 123 692.0 0 123 204 151 692.0 0 151 205 59 692.0 0 59 206 38 692.0 0 38 207 126 692.0 0 126 208 165 692.0 0 165 209 142 692.0 0 142 210 134 692.0 0 134 211 75 692.0 0 75 212 100 692.0 0 100 213 64 692.0 0 64 214 37 692.0 0 37 215 81 692.0 0 81 216 100 692.0 0 100 217 85 692.0 0 85 218 172 692.0 0 172 219 340 692.0 0 340 220 2771 692.0 0 2771 221 1156 692.0 0 1156 222 1938 692.0 0 1938 223 98 692.0 0 98 224 45 692.0 0 45 225 244 692.0 0 244 226 37 692.0 0 37 227 67 692.0 0 67 228 39 692.0 0 39 229 26 692.0 0 26 230 93 692.0 0 93 231 83 692.0 0 83 232 303 692.0 0 303 233 67 692.0 0 67 234 77 692.0 0 77 235 84 692.0 0 84 236 45 692.0 0 45 237 53 692.0 0 53 238 48 692.0 0 48 239 167 692.0 0 167 240 127 692.0 0 127 241 271 692.0 0 271 242 137 692.0 0 137 243 147 692.0 0 147 244 117 692.0 0 117 245 103 692.0 0 103 246 84 692.0 0 84 247 50 692.0 0 50 248 54 692.0 0 54 249 105 692.0 0 105 250 84 692.0 0 84 251 113 692.0 0 113 252 66 692.0 0 66 253 115 692.0 0 115 254 63 692.0 0 63 255 132 692.0 0 132 256 223 692.0 0 223 257 163 692.0 0 163 258 79 692.0 0 79 259 109 692.0 0 109 260 156 692.0 0 156 261 152 692.0 0 152 262 397 692.0 0 397 263 260 692.0 0 260 264 746 692.0 0 746 265 300 692.0 0 300 266 263 692.0 0 263 267 198 692.0 0 198 268 350 692.0 0 350 269 1442 692.0 0 1442 270 1417 692.0 0 1417 271 2885 692.0 0 2885 272 229 692.0 0 229 273 118 692.0 0 118 274 786 692.0 0 786 275 200 692.0 0 200 276 1073 692.0 0 1073 277 197 692.0 0 197 278 299 692.0 0 299 279 430 692.0 0 430 280 713 692.0 0 713 281 1566 692.0 0 1566 282 6863 692.0 0 6863 283 39350 692.0 0 39350 284 21297 692.0 0 21297 285 17194 692.0 0 17194 286 1235 692.0 0 1235 287 46 692.0 0 46 288 135 692.0 0 135 289 206 692.0 0 206 290 587 692.0 0 587 291 169 692.0 0 169 292 43 692.0 0 43 293 44 692.0 0 44 294 48 692.0 0 48 295 29 692.0 0 29 296 49 692.0 0 49 297 86 692.0 0 86 298 103 692.0 0 103 299 32 692.0 0 32 300 49 692.0 0 49 301 50 692.0 0 50 Finished in 8.31 s (54 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 153,034 Reads with adapters: 64,584 (42.2%) Reads that were too short: 144 (0.1%) Reads written (passing filters): 64,440 (42.1%) Total basepairs processed: 42,662,475 bp Total written (filtered): 17,511,270 bp (41.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 64584 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 7 0.0 3 0 0 0 5 2 18 31 0.0 3 0 0 10 20 1 19 288 0.0 3 0 194 31 43 20 20 63396 0.0 4 59098 3406 662 185 45 21 153 0.0 4 18 119 10 3 3 22 8 0.0 4 0 0 6 0 2 23 382 0.0 4 1 0 1 285 95 24 4 0.0 4 1 0 0 0 3 27 1 0.0 4 0 0 0 0 1 45 1 0.0 4 1 71 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 146 0.0 4 0 0 0 0 146 128 7 0.0 4 0 0 0 0 7 129 1 0.0 4 0 0 0 0 1 135 4 0.0 4 0 0 0 0 4 140 1 0.0 4 1 146 7 0.0 4 0 0 0 0 7 147 3 0.0 4 0 0 0 0 3 148 1 0.0 4 0 0 0 0 1 151 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 1 154 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 1 193 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 1 206 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 211 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 221 4 0.0 4 0 0 0 0 4 222 3 0.0 4 0 0 0 0 3 223 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 20 0.0 4 0 0 0 1 19 229 9 0.0 4 0 0 0 0 9 233 2 0.0 4 0 0 0 0 2 234 5 0.0 4 0 0 0 1 4 235 11 0.0 4 0 0 0 0 11 236 7 0.0 4 0 0 0 1 6 237 21 0.0 4 0 0 0 0 21 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 279 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 283 3 0.0 4 0 0 0 0 3 284 2 0.0 4 0 0 0 0 2 288 2 0.0 4 0 0 0 0 2 290 1 0.0 4 0 0 0 0 1 292 3 0.0 4 0 0 0 0 3 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.37 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 64,440 Reads with adapters: 15,244 (23.7%) Reads written (passing filters): 64,440 (100.0%) Total basepairs processed: 17,511,270 bp Total written (filtered): 17,344,224 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 15244 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.1% C: 9.4% G: 85.0% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 38 1006.9 0 38 4 13 251.7 0 13 5 1 62.9 0 1 6 1 15.7 0 1 8 26 1.0 0 26 9 2670 0.2 0 354 2316 10 8996 0.1 1 809 8187 11 3268 0.0 1 152 3116 12 38 0.0 1 0 38 13 52 0.0 1 0 52 14 5 0.0 1 1 4 15 47 0.0 1 29 18 16 3 0.0 1 3 17 1 0.0 1 1 70 5 0.0 1 4 1 121 1 0.0 1 1 150 40 0.0 1 32 8 189 1 0.0 1 0 1 208 30 0.0 1 25 5 209 2 0.0 1 2 210 1 0.0 1 1 223 2 0.0 1 2 257 1 0.0 1 1 260 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.