This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.89 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 241,679 (8.5%) Reads that were too short: 87,366 (3.1%) Reads written (passing filters): 154,313 (5.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 43,344,259 bp (5.1%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 241679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1084 692.0 0 1084 7 1932 692.0 0 1932 8 64383 692.0 0 64383 9 69601 692.0 0 69601 10 63 692.0 0 63 11 26 692.0 0 26 12 11 692.0 0 11 13 10 692.0 0 10 14 7 692.0 0 7 15 7 692.0 0 7 16 11 692.0 0 11 17 2 692.0 0 2 18 12 692.0 0 12 19 5 692.0 0 5 20 6 692.0 0 6 21 135 692.0 0 135 22 9 692.0 0 9 23 6 692.0 0 6 24 5 692.0 0 5 25 6 692.0 0 6 26 8 692.0 0 8 27 9 692.0 0 9 28 10 692.0 0 10 29 6 692.0 0 6 30 1 692.0 0 1 31 11 692.0 0 11 32 2 692.0 0 2 33 5 692.0 0 5 34 9 692.0 0 9 35 15 692.0 0 15 36 15 692.0 0 15 37 6 692.0 0 6 38 21 692.0 0 21 39 3 692.0 0 3 40 10 692.0 0 10 41 7 692.0 0 7 42 22 692.0 0 22 43 82 692.0 0 82 44 1 692.0 0 1 45 18 692.0 0 18 46 13 692.0 0 13 47 11 692.0 0 11 48 14 692.0 0 14 49 29 692.0 0 29 50 16 692.0 0 16 51 21 692.0 0 21 52 21 692.0 0 21 53 6 692.0 0 6 54 11 692.0 0 11 55 13 692.0 0 13 56 883 692.0 0 883 57 167 692.0 0 167 58 71 692.0 0 71 59 15 692.0 0 15 60 10 692.0 0 10 61 36 692.0 0 36 62 18 692.0 0 18 63 17 692.0 0 17 64 26 692.0 0 26 65 17 692.0 0 17 66 12 692.0 0 12 67 12 692.0 0 12 68 17 692.0 0 17 69 117 692.0 0 117 70 175 692.0 0 175 71 155 692.0 0 155 72 53 692.0 0 53 73 21 692.0 0 21 74 44 692.0 0 44 75 48 692.0 0 48 76 128 692.0 0 128 77 58 692.0 0 58 78 55 692.0 0 55 79 73 692.0 0 73 80 38 692.0 0 38 81 41 692.0 0 41 82 53 692.0 0 53 83 51 692.0 0 51 84 64 692.0 0 64 85 228 692.0 0 228 86 59 692.0 0 59 87 55 692.0 0 55 88 64 692.0 0 64 89 1500 692.0 0 1500 90 610 692.0 0 610 91 5006 692.0 0 5006 92 40 692.0 0 40 93 14 692.0 0 14 94 13 692.0 0 13 95 15 692.0 0 15 96 13 692.0 0 13 97 48 692.0 0 48 98 35 692.0 0 35 99 37 692.0 0 37 100 42 692.0 0 42 101 14 692.0 0 14 102 16 692.0 0 16 103 15 692.0 0 15 104 17 692.0 0 17 105 14 692.0 0 14 106 15 692.0 0 15 107 20 692.0 0 20 108 23 692.0 0 23 109 18 692.0 0 18 110 24 692.0 0 24 111 22 692.0 0 22 112 55 692.0 0 55 113 29 692.0 0 29 114 7 692.0 0 7 115 12 692.0 0 12 116 5 692.0 0 5 117 36 692.0 0 36 118 12 692.0 0 12 119 11 692.0 0 11 120 16 692.0 0 16 121 12 692.0 0 12 122 17 692.0 0 17 123 14 692.0 0 14 124 9 692.0 0 9 125 14 692.0 0 14 126 18 692.0 0 18 127 16 692.0 0 16 128 54 692.0 0 54 129 7 692.0 0 7 130 20 692.0 0 20 131 8 692.0 0 8 132 18 692.0 0 18 133 19 692.0 0 19 134 18 692.0 0 18 135 14 692.0 0 14 136 19 692.0 0 19 137 68 692.0 0 68 138 16 692.0 0 16 139 41 692.0 0 41 140 29 692.0 0 29 141 24 692.0 0 24 142 17 692.0 0 17 143 11 692.0 0 11 144 14 692.0 0 14 145 20 692.0 0 20 146 28 692.0 0 28 147 24 692.0 0 24 148 57 692.0 0 57 149 46 692.0 0 46 150 94 692.0 0 94 151 15 692.0 0 15 152 21 692.0 0 21 153 23 692.0 0 23 154 19 692.0 0 19 155 22 692.0 0 22 156 96 692.0 0 96 157 60 692.0 0 60 158 28 692.0 0 28 159 40 692.0 0 40 160 42 692.0 0 42 161 31 692.0 0 31 162 26 692.0 0 26 163 24 692.0 0 24 164 25 692.0 0 25 165 24 692.0 0 24 166 23 692.0 0 23 167 41 692.0 0 41 168 337 692.0 0 337 169 136 692.0 0 136 170 119 692.0 0 119 171 22 692.0 0 22 172 26 692.0 0 26 173 31 692.0 0 31 174 71 692.0 0 71 175 134 692.0 0 134 176 2003 692.0 0 2003 177 920 692.0 0 920 178 329 692.0 0 329 179 34 692.0 0 34 180 20 692.0 0 20 181 8 692.0 0 8 182 24 692.0 0 24 183 22 692.0 0 22 184 21 692.0 0 21 185 16 692.0 0 16 186 25 692.0 0 25 187 31 692.0 0 31 188 12 692.0 0 12 189 28 692.0 0 28 190 23 692.0 0 23 191 19 692.0 0 19 192 13 692.0 0 13 193 26 692.0 0 26 194 30 692.0 0 30 195 22 692.0 0 22 196 23 692.0 0 23 197 24 692.0 0 24 198 19 692.0 0 19 199 11 692.0 0 11 200 16 692.0 0 16 201 20 692.0 0 20 202 69 692.0 0 69 203 46 692.0 0 46 204 46 692.0 0 46 205 18 692.0 0 18 206 27 692.0 0 27 207 84 692.0 0 84 208 84 692.0 0 84 209 88 692.0 0 88 210 44 692.0 0 44 211 41 692.0 0 41 212 43 692.0 0 43 213 40 692.0 0 40 214 31 692.0 0 31 215 58 692.0 0 58 216 74 692.0 0 74 217 85 692.0 0 85 218 146 692.0 0 146 219 276 692.0 0 276 220 2837 692.0 0 2837 221 1278 692.0 0 1278 222 1913 692.0 0 1913 223 81 692.0 0 81 224 35 692.0 0 35 225 247 692.0 0 247 226 33 692.0 0 33 227 63 692.0 0 63 228 16 692.0 0 16 229 13 692.0 0 13 230 59 692.0 0 59 231 64 692.0 0 64 232 139 692.0 0 139 233 52 692.0 0 52 234 94 692.0 0 94 235 40 692.0 0 40 236 31 692.0 0 31 237 19 692.0 0 19 238 31 692.0 0 31 239 78 692.0 0 78 240 75 692.0 0 75 241 138 692.0 0 138 242 86 692.0 0 86 243 84 692.0 0 84 244 62 692.0 0 62 245 58 692.0 0 58 246 61 692.0 0 61 247 52 692.0 0 52 248 34 692.0 0 34 249 59 692.0 0 59 250 65 692.0 0 65 251 92 692.0 0 92 252 38 692.0 0 38 253 74 692.0 0 74 254 54 692.0 0 54 255 80 692.0 0 80 256 167 692.0 0 167 257 112 692.0 0 112 258 51 692.0 0 51 259 113 692.0 0 113 260 118 692.0 0 118 261 117 692.0 0 117 262 193 692.0 0 193 263 108 692.0 0 108 264 230 692.0 0 230 265 159 692.0 0 159 266 206 692.0 0 206 267 107 692.0 0 107 268 207 692.0 0 207 269 669 692.0 0 669 270 651 692.0 0 651 271 1243 692.0 0 1243 272 181 692.0 0 181 273 79 692.0 0 79 274 1154 692.0 0 1154 275 240 692.0 0 240 276 1576 692.0 0 1576 277 131 692.0 0 131 278 225 692.0 0 225 279 356 692.0 0 356 280 438 692.0 0 438 281 1286 692.0 0 1286 282 5320 692.0 0 5320 283 30533 692.0 0 30533 284 17650 692.0 0 17650 285 12228 692.0 0 12228 286 934 692.0 0 934 287 41 692.0 0 41 288 93 692.0 0 93 289 130 692.0 0 130 290 288 692.0 0 288 291 147 692.0 0 147 292 32 692.0 0 32 293 41 692.0 0 41 294 44 692.0 0 44 295 31 692.0 0 31 296 37 692.0 0 37 297 46 692.0 0 46 298 45 692.0 0 45 299 35 692.0 0 35 300 27 692.0 0 27 301 12 692.0 0 12 Finished in 7.43 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 154,313 Reads with adapters: 65,265 (42.3%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 65,246 (42.3%) Total basepairs processed: 43,344,259 bp Total written (filtered): 17,999,190 bp (41.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 65265 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 5 0.0 3 0 0 3 2 16 222 0.0 3 0 217 5 17 64697 0.0 3 63399 1249 45 4 18 140 0.0 3 12 123 5 19 42 0.0 3 0 0 35 7 20 72 0.0 3 0 0 0 72 32 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 101 12 0.0 3 0 0 0 12 102 29 0.0 3 0 0 0 29 103 1 0.0 3 0 0 0 1 105 6 0.0 3 0 0 0 6 113 1 0.0 3 0 0 0 1 116 1 0.0 3 1 119 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 161 1 0.0 3 1 167 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 194 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 2 0.0 3 0 0 1 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.30 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 65,246 Reads with adapters: 381 (0.6%) Reads written (passing filters): 65,246 (100.0%) Total basepairs processed: 17,999,190 bp Total written (filtered): 17,997,869 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 381 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.8% C: 7.1% G: 35.7% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 227 1019.5 0 227 4 148 254.9 0 148 5 3 63.7 0 3 11 3 0.0 1 0 3