This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.60 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 144,343 (5.1%) Reads that were too short: 31,519 (1.1%) Reads written (passing filters): 112,824 (4.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 26,307,829 bp (3.1%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 144343 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2649 692.0 0 2649 7 28809 692.0 0 28809 8 20141 692.0 0 20141 9 61 692.0 0 61 10 47 692.0 0 47 11 46 692.0 0 46 12 36 692.0 0 36 13 41 692.0 0 41 14 47 692.0 0 47 15 38 692.0 0 38 16 39 692.0 0 39 17 56 692.0 0 56 18 81 692.0 0 81 19 58 692.0 0 58 20 176 692.0 0 176 21 58 692.0 0 58 22 29 692.0 0 29 23 50 692.0 0 50 24 34 692.0 0 34 25 31 692.0 0 31 26 30 692.0 0 30 27 49 692.0 0 49 28 44 692.0 0 44 29 57 692.0 0 57 30 91 692.0 0 91 31 32 692.0 0 32 32 25 692.0 0 25 33 42 692.0 0 42 34 65 692.0 0 65 35 31 692.0 0 31 36 72 692.0 0 72 37 58 692.0 0 58 38 63 692.0 0 63 39 44 692.0 0 44 40 59 692.0 0 59 41 47 692.0 0 47 42 204 692.0 0 204 43 55 692.0 0 55 44 38 692.0 0 38 45 61 692.0 0 61 46 199 692.0 0 199 47 42 692.0 0 42 48 44 692.0 0 44 49 34 692.0 0 34 50 61 692.0 0 61 51 62 692.0 0 62 52 56 692.0 0 56 53 61 692.0 0 61 54 50 692.0 0 50 55 55 692.0 0 55 56 97 692.0 0 97 57 38 692.0 0 38 58 56 692.0 0 56 59 56 692.0 0 56 60 78 692.0 0 78 61 34 692.0 0 34 62 48 692.0 0 48 63 69 692.0 0 69 64 104 692.0 0 104 65 147 692.0 0 147 66 249 692.0 0 249 67 455 692.0 0 455 68 163 692.0 0 163 69 252 692.0 0 252 70 435 692.0 0 435 71 103 692.0 0 103 72 134 692.0 0 134 73 207 692.0 0 207 74 187 692.0 0 187 75 206 692.0 0 206 76 210 692.0 0 210 77 290 692.0 0 290 78 270 692.0 0 270 79 521 692.0 0 521 80 6091 692.0 0 6091 81 8401 692.0 0 8401 82 11877 692.0 0 11877 83 220 692.0 0 220 84 118 692.0 0 118 85 98 692.0 0 98 86 132 692.0 0 132 87 88 692.0 0 88 88 81 692.0 0 81 89 156 692.0 0 156 90 46 692.0 0 46 91 32 692.0 0 32 92 38 692.0 0 38 93 63 692.0 0 63 94 41 692.0 0 41 95 83 692.0 0 83 96 140 692.0 0 140 97 65 692.0 0 65 98 27 692.0 0 27 99 55 692.0 0 55 100 41 692.0 0 41 101 27 692.0 0 27 102 40 692.0 0 40 103 49 692.0 0 49 104 31 692.0 0 31 105 39 692.0 0 39 106 37 692.0 0 37 107 42 692.0 0 42 108 29 692.0 0 29 109 29 692.0 0 29 110 49 692.0 0 49 111 43 692.0 0 43 112 50 692.0 0 50 113 55 692.0 0 55 114 57 692.0 0 57 115 39 692.0 0 39 116 36 692.0 0 36 117 90 692.0 0 90 118 116 692.0 0 116 119 178 692.0 0 178 120 200 692.0 0 200 121 226 692.0 0 226 122 197 692.0 0 197 123 54 692.0 0 54 124 112 692.0 0 112 125 157 692.0 0 157 126 152 692.0 0 152 127 223 692.0 0 223 128 685 692.0 0 685 129 126 692.0 0 126 130 30 692.0 0 30 131 51 692.0 0 51 132 44 692.0 0 44 133 61 692.0 0 61 134 57 692.0 0 57 135 71 692.0 0 71 136 113 692.0 0 113 137 41 692.0 0 41 138 46 692.0 0 46 139 64 692.0 0 64 140 42 692.0 0 42 141 79 692.0 0 79 142 57 692.0 0 57 143 50 692.0 0 50 144 74 692.0 0 74 145 58 692.0 0 58 146 100 692.0 0 100 147 76 692.0 0 76 148 80 692.0 0 80 149 89 692.0 0 89 150 146 692.0 0 146 151 35 692.0 0 35 152 68 692.0 0 68 153 51 692.0 0 51 154 67 692.0 0 67 155 44 692.0 0 44 156 63 692.0 0 63 157 38 692.0 0 38 158 41 692.0 0 41 159 48 692.0 0 48 160 49 692.0 0 49 161 72 692.0 0 72 162 67 692.0 0 67 163 51 692.0 0 51 164 72 692.0 0 72 165 91 692.0 0 91 166 71 692.0 0 71 167 261 692.0 0 261 168 125 692.0 0 125 169 1015 692.0 0 1015 170 62 692.0 0 62 171 76 692.0 0 76 172 69 692.0 0 69 173 106 692.0 0 106 174 139 692.0 0 139 175 268 692.0 0 268 176 105 692.0 0 105 177 129 692.0 0 129 178 145 692.0 0 145 179 94 692.0 0 94 180 120 692.0 0 120 181 121 692.0 0 121 182 113 692.0 0 113 183 126 692.0 0 126 184 141 692.0 0 141 185 220 692.0 0 220 186 244 692.0 0 244 187 997 692.0 0 997 188 6775 692.0 0 6775 189 2248 692.0 0 2248 190 4137 692.0 0 4137 191 480 692.0 0 480 192 610 692.0 0 610 193 479 692.0 0 479 194 493 692.0 0 493 195 138 692.0 0 138 196 188 692.0 0 188 197 70 692.0 0 70 198 72 692.0 0 72 199 116 692.0 0 116 200 70 692.0 0 70 201 87 692.0 0 87 202 90 692.0 0 90 203 85 692.0 0 85 204 108 692.0 0 108 205 122 692.0 0 122 206 1073 692.0 0 1073 207 1511 692.0 0 1511 208 2178 692.0 0 2178 209 216 692.0 0 216 210 135 692.0 0 135 211 451 692.0 0 451 212 472 692.0 0 472 213 921 692.0 0 921 214 283 692.0 0 283 215 51 692.0 0 51 216 73 692.0 0 73 217 39 692.0 0 39 218 51 692.0 0 51 219 64 692.0 0 64 220 41 692.0 0 41 221 60 692.0 0 60 222 157 692.0 0 157 223 96 692.0 0 96 224 54 692.0 0 54 225 67 692.0 0 67 226 97 692.0 0 97 227 80 692.0 0 80 228 88 692.0 0 88 229 140 692.0 0 140 230 181 692.0 0 181 231 136 692.0 0 136 232 87 692.0 0 87 233 75 692.0 0 75 234 87 692.0 0 87 235 84 692.0 0 84 236 60 692.0 0 60 237 98 692.0 0 98 238 79 692.0 0 79 239 74 692.0 0 74 240 96 692.0 0 96 241 89 692.0 0 89 242 68 692.0 0 68 243 64 692.0 0 64 244 95 692.0 0 95 245 53 692.0 0 53 246 65 692.0 0 65 247 104 692.0 0 104 248 121 692.0 0 121 249 66 692.0 0 66 250 78 692.0 0 78 251 110 692.0 0 110 252 136 692.0 0 136 253 87 692.0 0 87 254 117 692.0 0 117 255 138 692.0 0 138 256 164 692.0 0 164 257 333 692.0 0 333 258 268 692.0 0 268 259 145 692.0 0 145 260 229 692.0 0 229 261 220 692.0 0 220 262 142 692.0 0 142 263 97 692.0 0 97 264 105 692.0 0 105 265 90 692.0 0 90 266 139 692.0 0 139 267 153 692.0 0 153 268 788 692.0 0 788 269 745 692.0 0 745 270 1555 692.0 0 1555 271 2811 692.0 0 2811 272 3091 692.0 0 3091 273 2860 692.0 0 2860 274 475 692.0 0 475 275 119 692.0 0 119 276 83 692.0 0 83 277 121 692.0 0 121 278 103 692.0 0 103 279 91 692.0 0 91 280 116 692.0 0 116 281 95 692.0 0 95 282 873 692.0 0 873 283 451 692.0 0 451 284 631 692.0 0 631 285 76 692.0 0 76 286 87 692.0 0 87 287 94 692.0 0 94 288 102 692.0 0 102 289 140 692.0 0 140 290 88 692.0 0 88 291 167 692.0 0 167 292 518 692.0 0 518 293 316 692.0 0 316 294 309 692.0 0 309 295 177 692.0 0 177 296 111 692.0 0 111 297 135 692.0 0 135 298 134 692.0 0 134 299 144 692.0 0 144 300 347 692.0 0 347 301 230 692.0 0 230 Finished in 5.46 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 112,824 Reads with adapters: 21,106 (18.7%) Reads that were too short: 130 (0.1%) Reads written (passing filters): 20,976 (18.6%) Total basepairs processed: 26,307,829 bp Total written (filtered): 5,719,591 bp (21.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21106 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 11 0.0 3 0 0 0 1 10 18 12 0.0 3 0 0 8 2 2 19 276 0.0 3 0 216 24 25 11 20 20451 0.0 4 19082 1089 219 46 15 21 45 0.0 4 12 32 1 22 17 0.0 4 3 1 4 3 6 23 95 0.0 4 0 0 0 68 27 24 3 0.0 4 0 0 0 0 3 27 1 0.0 4 0 0 0 0 1 29 3 0.0 4 2 0 0 0 1 30 2 0.0 4 0 0 0 0 2 32 1 0.0 4 0 0 0 0 1 33 2 0.0 4 2 39 1 0.0 4 0 0 0 0 1 50 2 0.0 4 0 0 0 0 2 55 11 0.0 4 0 0 0 0 11 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 1 103 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 127 33 0.0 4 0 0 0 0 33 129 1 0.0 4 0 0 0 0 1 130 8 0.0 4 0 0 0 1 7 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 154 2 0.0 4 0 0 0 0 2 155 45 0.0 4 0 0 0 1 44 156 27 0.0 4 0 0 1 0 26 157 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 192 2 0.0 4 0 0 0 0 2 200 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 210 4 0.0 4 0 0 0 0 4 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 1 1 237 2 0.0 4 0 1 0 0 1 238 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.76 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 20,976 Reads with adapters: 3,654 (17.4%) Reads written (passing filters): 20,976 (100.0%) Total basepairs processed: 5,719,591 bp Total written (filtered): 5,669,104 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3654 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.2% C: 9.6% G: 83.6% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 43 327.8 0 43 4 3 81.9 0 3 9 16 0.1 0 1 15 10 171 0.0 1 17 154 11 3341 0.0 1 170 3171 12 18 0.0 1 1 17 14 3 0.0 1 0 3 81 1 0.0 1 0 1 126 3 0.0 1 0 3 135 2 0.0 1 1 1 139 2 0.0 1 2 151 2 0.0 1 1 1 185 1 0.0 1 1 200 4 0.0 1 3 1 201 2 0.0 1 2 209 37 0.0 1 33 4 210 1 0.0 1 1 211 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.