This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.08 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 132,497 (4.7%) Reads that were too short: 23,137 (0.8%) Reads written (passing filters): 109,360 (3.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 25,830,803 bp (3.0%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 132497 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1890 692.0 0 1890 7 23276 692.0 0 23276 8 27664 692.0 0 27664 9 43 692.0 0 43 10 26 692.0 0 26 11 33 692.0 0 33 12 27 692.0 0 27 13 19 692.0 0 19 14 30 692.0 0 30 15 32 692.0 0 32 16 28 692.0 0 28 17 41 692.0 0 41 18 40 692.0 0 40 19 21 692.0 0 21 20 41 692.0 0 41 21 28 692.0 0 28 22 31 692.0 0 31 23 16 692.0 0 16 24 29 692.0 0 29 25 21 692.0 0 21 26 30 692.0 0 30 27 31 692.0 0 31 28 35 692.0 0 35 29 24 692.0 0 24 30 66 692.0 0 66 31 32 692.0 0 32 32 25 692.0 0 25 33 32 692.0 0 32 34 64 692.0 0 64 35 32 692.0 0 32 36 74 692.0 0 74 37 70 692.0 0 70 38 64 692.0 0 64 39 34 692.0 0 34 40 41 692.0 0 41 41 42 692.0 0 42 42 224 692.0 0 224 43 36 692.0 0 36 44 13 692.0 0 13 45 44 692.0 0 44 46 182 692.0 0 182 47 23 692.0 0 23 48 197 692.0 0 197 49 51 692.0 0 51 50 42 692.0 0 42 51 37 692.0 0 37 52 42 692.0 0 42 53 29 692.0 0 29 54 44 692.0 0 44 55 25 692.0 0 25 56 53 692.0 0 53 57 25 692.0 0 25 58 44 692.0 0 44 59 30 692.0 0 30 60 62 692.0 0 62 61 32 692.0 0 32 62 45 692.0 0 45 63 51 692.0 0 51 64 87 692.0 0 87 65 131 692.0 0 131 66 244 692.0 0 244 67 488 692.0 0 488 68 125 692.0 0 125 69 271 692.0 0 271 70 397 692.0 0 397 71 90 692.0 0 90 72 112 692.0 0 112 73 168 692.0 0 168 74 132 692.0 0 132 75 205 692.0 0 205 76 199 692.0 0 199 77 221 692.0 0 221 78 236 692.0 0 236 79 431 692.0 0 431 80 5823 692.0 0 5823 81 8353 692.0 0 8353 82 12246 692.0 0 12246 83 93 692.0 0 93 84 71 692.0 0 71 85 26 692.0 0 26 86 31 692.0 0 31 87 44 692.0 0 44 88 62 692.0 0 62 89 82 692.0 0 82 90 29 692.0 0 29 91 36 692.0 0 36 92 37 692.0 0 37 93 22 692.0 0 22 94 26 692.0 0 26 95 37 692.0 0 37 96 73 692.0 0 73 97 54 692.0 0 54 98 38 692.0 0 38 99 36 692.0 0 36 100 18 692.0 0 18 101 19 692.0 0 19 102 33 692.0 0 33 103 39 692.0 0 39 104 34 692.0 0 34 105 26 692.0 0 26 106 26 692.0 0 26 107 35 692.0 0 35 108 28 692.0 0 28 109 17 692.0 0 17 110 36 692.0 0 36 111 40 692.0 0 40 112 48 692.0 0 48 113 46 692.0 0 46 114 37 692.0 0 37 115 30 692.0 0 30 116 14 692.0 0 14 117 60 692.0 0 60 118 54 692.0 0 54 119 69 692.0 0 69 120 96 692.0 0 96 121 124 692.0 0 124 122 127 692.0 0 127 123 52 692.0 0 52 124 61 692.0 0 61 125 56 692.0 0 56 126 79 692.0 0 79 127 158 692.0 0 158 128 662 692.0 0 662 129 93 692.0 0 93 130 34 692.0 0 34 131 43 692.0 0 43 132 43 692.0 0 43 133 38 692.0 0 38 134 42 692.0 0 42 135 68 692.0 0 68 136 118 692.0 0 118 137 35 692.0 0 35 138 43 692.0 0 43 139 56 692.0 0 56 140 23 692.0 0 23 141 39 692.0 0 39 142 39 692.0 0 39 143 35 692.0 0 35 144 78 692.0 0 78 145 44 692.0 0 44 146 77 692.0 0 77 147 50 692.0 0 50 148 41 692.0 0 41 149 57 692.0 0 57 150 137 692.0 0 137 151 28 692.0 0 28 152 51 692.0 0 51 153 53 692.0 0 53 154 53 692.0 0 53 155 37 692.0 0 37 156 41 692.0 0 41 157 23 692.0 0 23 158 30 692.0 0 30 159 31 692.0 0 31 160 41 692.0 0 41 161 54 692.0 0 54 162 60 692.0 0 60 163 55 692.0 0 55 164 63 692.0 0 63 165 99 692.0 0 99 166 65 692.0 0 65 167 245 692.0 0 245 168 89 692.0 0 89 169 1146 692.0 0 1146 170 47 692.0 0 47 171 62 692.0 0 62 172 58 692.0 0 58 173 76 692.0 0 76 174 142 692.0 0 142 175 235 692.0 0 235 176 87 692.0 0 87 177 122 692.0 0 122 178 103 692.0 0 103 179 86 692.0 0 86 180 74 692.0 0 74 181 103 692.0 0 103 182 96 692.0 0 96 183 89 692.0 0 89 184 106 692.0 0 106 185 152 692.0 0 152 186 207 692.0 0 207 187 910 692.0 0 910 188 6547 692.0 0 6547 189 2277 692.0 0 2277 190 4336 692.0 0 4336 191 341 692.0 0 341 192 422 692.0 0 422 193 247 692.0 0 247 194 266 692.0 0 266 195 86 692.0 0 86 196 109 692.0 0 109 197 36 692.0 0 36 198 40 692.0 0 40 199 54 692.0 0 54 200 43 692.0 0 43 201 61 692.0 0 61 202 51 692.0 0 51 203 64 692.0 0 64 204 67 692.0 0 67 205 84 692.0 0 84 206 882 692.0 0 882 207 1357 692.0 0 1357 208 1978 692.0 0 1978 209 161 692.0 0 161 210 27 692.0 0 27 211 164 692.0 0 164 212 141 692.0 0 141 213 286 692.0 0 286 214 106 692.0 0 106 215 41 692.0 0 41 216 55 692.0 0 55 217 25 692.0 0 25 218 32 692.0 0 32 219 58 692.0 0 58 220 38 692.0 0 38 221 34 692.0 0 34 222 153 692.0 0 153 223 53 692.0 0 53 224 28 692.0 0 28 225 42 692.0 0 42 226 74 692.0 0 74 227 61 692.0 0 61 228 48 692.0 0 48 229 71 692.0 0 71 230 65 692.0 0 65 231 63 692.0 0 63 232 50 692.0 0 50 233 42 692.0 0 42 234 48 692.0 0 48 235 36 692.0 0 36 236 30 692.0 0 30 237 57 692.0 0 57 238 43 692.0 0 43 239 31 692.0 0 31 240 33 692.0 0 33 241 52 692.0 0 52 242 37 692.0 0 37 243 40 692.0 0 40 244 44 692.0 0 44 245 43 692.0 0 43 246 34 692.0 0 34 247 56 692.0 0 56 248 63 692.0 0 63 249 49 692.0 0 49 250 40 692.0 0 40 251 79 692.0 0 79 252 78 692.0 0 78 253 58 692.0 0 58 254 64 692.0 0 64 255 64 692.0 0 64 256 107 692.0 0 107 257 220 692.0 0 220 258 270 692.0 0 270 259 83 692.0 0 83 260 102 692.0 0 102 261 172 692.0 0 172 262 78 692.0 0 78 263 70 692.0 0 70 264 77 692.0 0 77 265 50 692.0 0 50 266 78 692.0 0 78 267 78 692.0 0 78 268 325 692.0 0 325 269 232 692.0 0 232 270 488 692.0 0 488 271 2994 692.0 0 2994 272 3950 692.0 0 3950 273 1736 692.0 0 1736 274 277 692.0 0 277 275 77 692.0 0 77 276 34 692.0 0 34 277 69 692.0 0 69 278 48 692.0 0 48 279 63 692.0 0 63 280 58 692.0 0 58 281 63 692.0 0 63 282 663 692.0 0 663 283 344 692.0 0 344 284 477 692.0 0 477 285 59 692.0 0 59 286 70 692.0 0 70 287 66 692.0 0 66 288 63 692.0 0 63 289 75 692.0 0 75 290 63 692.0 0 63 291 110 692.0 0 110 292 503 692.0 0 503 293 293 692.0 0 293 294 296 692.0 0 296 295 105 692.0 0 105 296 78 692.0 0 78 297 82 692.0 0 82 298 105 692.0 0 105 299 77 692.0 0 77 300 183 692.0 0 183 301 146 692.0 0 146 Finished in 4.76 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 109,360 Reads with adapters: 24,382 (22.3%) Reads that were too short: 81 (0.1%) Reads written (passing filters): 24,301 (22.2%) Total basepairs processed: 25,830,803 bp Total written (filtered): 6,709,995 bp (26.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24382 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 130 0.0 3 0 127 3 17 22415 0.0 3 22016 383 13 3 18 48 0.0 3 5 39 4 19 20 0.0 3 4 11 5 20 1557 0.0 3 1440 38 1 78 21 3 0.0 3 2 0 0 1 23 11 0.0 3 0 0 0 11 25 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 36 1 0.0 3 1 40 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 53 5 0.0 3 0 0 0 5 63 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 2 0.0 3 0 0 0 2 67 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 75 14 0.0 3 0 0 0 14 76 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 86 24 0.0 3 0 0 0 24 87 6 0.0 3 0 0 0 6 89 5 0.0 3 2 0 0 3 90 2 0.0 3 1 1 91 4 0.0 3 1 0 0 3 92 8 0.0 3 0 0 0 8 93 1 0.0 3 0 0 0 1 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 109 3 0.0 3 0 0 0 3 110 2 0.0 3 0 0 0 2 111 12 0.0 3 0 0 0 12 114 1 0.0 3 0 0 0 1 115 4 0.0 3 0 0 0 4 119 1 0.0 3 0 0 0 1 120 1 0.0 3 1 122 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 151 6 0.0 3 0 0 0 6 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 1 1 162 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 206 35 0.0 3 0 0 0 35 207 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 24,301 Reads with adapters: 85 (0.3%) Reads written (passing filters): 24,301 (100.0%) Total basepairs processed: 6,709,995 bp Total written (filtered): 6,709,731 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 85 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.4% C: 17.6% G: 27.1% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 77 379.7 0 77 4 7 94.9 0 7 5 1 23.7 0 1