This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.11 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 109,012 (3.8%) Reads that were too short: 43,686 (1.5%) Reads written (passing filters): 65,326 (2.3%) Total basepairs processed: 853,219,115 bp Total written (filtered): 11,812,909 bp (1.4%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 109012 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 184 692.0 0 184 7 5518 692.0 0 5518 8 88 692.0 0 88 9 67 692.0 0 67 10 82 692.0 0 82 11 57 692.0 0 57 12 57 692.0 0 57 13 124 692.0 0 124 14 67 692.0 0 67 15 57 692.0 0 57 16 52 692.0 0 52 17 60 692.0 0 60 18 58 692.0 0 58 19 55 692.0 0 55 20 61 692.0 0 61 21 57 692.0 0 57 22 57 692.0 0 57 23 73 692.0 0 73 24 55 692.0 0 55 25 66 692.0 0 66 26 68 692.0 0 68 27 72 692.0 0 72 28 63 692.0 0 63 29 63 692.0 0 63 30 58 692.0 0 58 31 55 692.0 0 55 32 69 692.0 0 69 33 81 692.0 0 81 34 70 692.0 0 70 35 62 692.0 0 62 36 56 692.0 0 56 37 66 692.0 0 66 38 56 692.0 0 56 39 73 692.0 0 73 40 60 692.0 0 60 41 72 692.0 0 72 42 61 692.0 0 61 43 89 692.0 0 89 44 63 692.0 0 63 45 87 692.0 0 87 46 75 692.0 0 75 47 66 692.0 0 66 48 75 692.0 0 75 49 59 692.0 0 59 50 82 692.0 0 82 51 69 692.0 0 69 52 73 692.0 0 73 53 60 692.0 0 60 54 54 692.0 0 54 55 52 692.0 0 52 56 87 692.0 0 87 57 98 692.0 0 98 58 57 692.0 0 57 59 50 692.0 0 50 60 57 692.0 0 57 61 66 692.0 0 66 62 62 692.0 0 62 63 70 692.0 0 70 64 62 692.0 0 62 65 63 692.0 0 63 66 83 692.0 0 83 67 64 692.0 0 64 68 59 692.0 0 59 69 64 692.0 0 64 70 85 692.0 0 85 71 64 692.0 0 64 72 87 692.0 0 87 73 93 692.0 0 93 74 256 692.0 0 256 75 232 692.0 0 232 76 468 692.0 0 468 77 229 692.0 0 229 78 316 692.0 0 316 79 104 692.0 0 104 80 80 692.0 0 80 81 73 692.0 0 73 82 69 692.0 0 69 83 84 692.0 0 84 84 77 692.0 0 77 85 51 692.0 0 51 86 63 692.0 0 63 87 58 692.0 0 58 88 72 692.0 0 72 89 68 692.0 0 68 90 62 692.0 0 62 91 100 692.0 0 100 92 65 692.0 0 65 93 83 692.0 0 83 94 65 692.0 0 65 95 83 692.0 0 83 96 111 692.0 0 111 97 113 692.0 0 113 98 74 692.0 0 74 99 77 692.0 0 77 100 82 692.0 0 82 101 64 692.0 0 64 102 86 692.0 0 86 103 117 692.0 0 117 104 103 692.0 0 103 105 153 692.0 0 153 106 233 692.0 0 233 107 897 692.0 0 897 108 237 692.0 0 237 109 328 692.0 0 328 110 112 692.0 0 112 111 100 692.0 0 100 112 101 692.0 0 101 113 159 692.0 0 159 114 122 692.0 0 122 115 140 692.0 0 140 116 121 692.0 0 121 117 134 692.0 0 134 118 142 692.0 0 142 119 181 692.0 0 181 120 307 692.0 0 307 121 335 692.0 0 335 122 206 692.0 0 206 123 525 692.0 0 525 124 689 692.0 0 689 125 2349 692.0 0 2349 126 197 692.0 0 197 127 241 692.0 0 241 128 304 692.0 0 304 129 267 692.0 0 267 130 258 692.0 0 258 131 324 692.0 0 324 132 358 692.0 0 358 133 936 692.0 0 936 134 3129 692.0 0 3129 135 5987 692.0 0 5987 136 13337 692.0 0 13337 137 301 692.0 0 301 138 234 692.0 0 234 139 247 692.0 0 247 140 213 692.0 0 213 141 249 692.0 0 249 142 256 692.0 0 256 143 138 692.0 0 138 144 141 692.0 0 141 145 164 692.0 0 164 146 175 692.0 0 175 147 404 692.0 0 404 148 286 692.0 0 286 149 314 692.0 0 314 150 137 692.0 0 137 151 151 692.0 0 151 152 182 692.0 0 182 153 236 692.0 0 236 154 644 692.0 0 644 155 895 692.0 0 895 156 1237 692.0 0 1237 157 2389 692.0 0 2389 158 1360 692.0 0 1360 159 2412 692.0 0 2412 160 195 692.0 0 195 161 205 692.0 0 205 162 209 692.0 0 209 163 170 692.0 0 170 164 217 692.0 0 217 165 237 692.0 0 237 166 337 692.0 0 337 167 218 692.0 0 218 168 94 692.0 0 94 169 135 692.0 0 135 170 93 692.0 0 93 171 83 692.0 0 83 172 110 692.0 0 110 173 147 692.0 0 147 174 150 692.0 0 150 175 147 692.0 0 147 176 95 692.0 0 95 177 80 692.0 0 80 178 70 692.0 0 70 179 94 692.0 0 94 180 110 692.0 0 110 181 154 692.0 0 154 182 151 692.0 0 151 183 120 692.0 0 120 184 97 692.0 0 97 185 133 692.0 0 133 186 290 692.0 0 290 187 144 692.0 0 144 188 139 692.0 0 139 189 116 692.0 0 116 190 97 692.0 0 97 191 147 692.0 0 147 192 125 692.0 0 125 193 123 692.0 0 123 194 139 692.0 0 139 195 87 692.0 0 87 196 108 692.0 0 108 197 112 692.0 0 112 198 74 692.0 0 74 199 104 692.0 0 104 200 107 692.0 0 107 201 129 692.0 0 129 202 137 692.0 0 137 203 116 692.0 0 116 204 126 692.0 0 126 205 110 692.0 0 110 206 123 692.0 0 123 207 164 692.0 0 164 208 161 692.0 0 161 209 127 692.0 0 127 210 106 692.0 0 106 211 108 692.0 0 108 212 135 692.0 0 135 213 239 692.0 0 239 214 188 692.0 0 188 215 204 692.0 0 204 216 199 692.0 0 199 217 125 692.0 0 125 218 102 692.0 0 102 219 171 692.0 0 171 220 212 692.0 0 212 221 183 692.0 0 183 222 229 692.0 0 229 223 107 692.0 0 107 224 102 692.0 0 102 225 195 692.0 0 195 226 122 692.0 0 122 227 121 692.0 0 121 228 160 692.0 0 160 229 105 692.0 0 105 230 162 692.0 0 162 231 149 692.0 0 149 232 243 692.0 0 243 233 183 692.0 0 183 234 194 692.0 0 194 235 115 692.0 0 115 236 92 692.0 0 92 237 131 692.0 0 131 238 108 692.0 0 108 239 115 692.0 0 115 240 125 692.0 0 125 241 150 692.0 0 150 242 144 692.0 0 144 243 138 692.0 0 138 244 121 692.0 0 121 245 121 692.0 0 121 246 111 692.0 0 111 247 97 692.0 0 97 248 107 692.0 0 107 249 93 692.0 0 93 250 147 692.0 0 147 251 218 692.0 0 218 252 693 692.0 0 693 253 291 692.0 0 291 254 400 692.0 0 400 255 149 692.0 0 149 256 143 692.0 0 143 257 165 692.0 0 165 258 162 692.0 0 162 259 142 692.0 0 142 260 168 692.0 0 168 261 151 692.0 0 151 262 179 692.0 0 179 263 160 692.0 0 160 264 136 692.0 0 136 265 120 692.0 0 120 266 155 692.0 0 155 267 177 692.0 0 177 268 404 692.0 0 404 269 299 692.0 0 299 270 558 692.0 0 558 271 678 692.0 0 678 272 626 692.0 0 626 273 263 692.0 0 263 274 496 692.0 0 496 275 370 692.0 0 370 276 1416 692.0 0 1416 277 1386 692.0 0 1386 278 1573 692.0 0 1573 279 465 692.0 0 465 280 344 692.0 0 344 281 342 692.0 0 342 282 445 692.0 0 445 283 242 692.0 0 242 284 251 692.0 0 251 285 342 692.0 0 342 286 167 692.0 0 167 287 193 692.0 0 193 288 270 692.0 0 270 289 414 692.0 0 414 290 420 692.0 0 420 291 369 692.0 0 369 292 304 692.0 0 304 293 372 692.0 0 372 294 578 692.0 0 578 295 8833 692.0 0 8833 296 4573 692.0 0 4573 297 5415 692.0 0 5415 298 123 692.0 0 123 299 116 692.0 0 116 300 130 692.0 0 130 301 277 692.0 0 277 Finished in 2.66 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 65,326 Reads with adapters: 2,495 (3.8%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 2,454 (3.8%) Total basepairs processed: 11,812,909 bp Total written (filtered): 670,806 bp (5.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2495 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 3 0.0 3 0 0 2 0 1 19 22 0.0 3 0 21 1 20 2401 0.0 4 2123 246 23 8 1 21 7 0.0 4 2 1 1 2 1 22 3 0.0 4 1 1 0 0 1 23 6 0.0 4 0 0 0 3 3 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 1 0 1 70 1 0.0 4 0 0 0 1 81 6 0.0 4 0 0 0 1 5 82 7 0.0 4 0 0 0 0 7 83 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 97 1 0.0 4 0 0 0 0 1 102 5 0.0 4 0 0 0 0 5 104 1 0.0 4 1 105 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 2,454 Reads with adapters: 95 (3.9%) Reads written (passing filters): 2,454 (100.0%) Total basepairs processed: 670,806 bp Total written (filtered): 668,790 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 95 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.4% C: 16.8% G: 72.6% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 7 38.3 0 7 10 1 0.0 1 0 1 11 58 0.0 1 3 55 12 23 0.0 1 2 21 16 1 0.0 1 0 1 152 1 0.0 1 1 210 3 0.0 1 3 273 1 0.0 1 0 1