This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.27 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 76,290 (2.7%) Reads that were too short: 28,997 (1.0%) Reads written (passing filters): 47,293 (1.7%) Total basepairs processed: 853,219,115 bp Total written (filtered): 9,020,401 bp (1.1%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 76290 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 354 692.0 0 354 7 249 692.0 0 249 8 6185 692.0 0 6185 9 4516 692.0 0 4516 10 18 692.0 0 18 11 3 692.0 0 3 12 5 692.0 0 5 13 22 692.0 0 22 14 70 692.0 0 70 15 197 692.0 0 197 16 31 692.0 0 31 17 17 692.0 0 17 18 48 692.0 0 48 19 59 692.0 0 59 20 43 692.0 0 43 21 14 692.0 0 14 22 1 692.0 0 1 24 2 692.0 0 2 25 2 692.0 0 2 26 2 692.0 0 2 27 9 692.0 0 9 28 9 692.0 0 9 29 10 692.0 0 10 31 2 692.0 0 2 32 6 692.0 0 6 33 10 692.0 0 10 34 13 692.0 0 13 35 6 692.0 0 6 36 7 692.0 0 7 37 5 692.0 0 5 38 10 692.0 0 10 39 4 692.0 0 4 40 9 692.0 0 9 41 10 692.0 0 10 42 15 692.0 0 15 43 96 692.0 0 96 44 333 692.0 0 333 45 364 692.0 0 364 46 101 692.0 0 101 47 15 692.0 0 15 48 12 692.0 0 12 49 10 692.0 0 10 50 17 692.0 0 17 51 16 692.0 0 16 52 17 692.0 0 17 53 21 692.0 0 21 54 11 692.0 0 11 55 7 692.0 0 7 56 24 692.0 0 24 57 13 692.0 0 13 58 16 692.0 0 16 59 66 692.0 0 66 60 59 692.0 0 59 61 30 692.0 0 30 62 17 692.0 0 17 63 9 692.0 0 9 64 10 692.0 0 10 65 36 692.0 0 36 66 35 692.0 0 35 67 35 692.0 0 35 68 82 692.0 0 82 69 36 692.0 0 36 70 28 692.0 0 28 71 51 692.0 0 51 72 71 692.0 0 71 73 43 692.0 0 43 74 74 692.0 0 74 75 101 692.0 0 101 76 223 692.0 0 223 77 2207 692.0 0 2207 78 2539 692.0 0 2539 79 4379 692.0 0 4379 80 45 692.0 0 45 81 18 692.0 0 18 82 38 692.0 0 38 83 17 692.0 0 17 84 31 692.0 0 31 85 28 692.0 0 28 86 49 692.0 0 49 87 6 692.0 0 6 88 4 692.0 0 4 89 7 692.0 0 7 90 13 692.0 0 13 91 33 692.0 0 33 92 27 692.0 0 27 93 37 692.0 0 37 94 53 692.0 0 53 95 22 692.0 0 22 96 32 692.0 0 32 97 7 692.0 0 7 98 5 692.0 0 5 99 8 692.0 0 8 100 7 692.0 0 7 101 10 692.0 0 10 102 15 692.0 0 15 103 44 692.0 0 44 104 23 692.0 0 23 105 10 692.0 0 10 106 11 692.0 0 11 107 6 692.0 0 6 108 39 692.0 0 39 109 54 692.0 0 54 110 7 692.0 0 7 111 13 692.0 0 13 112 13 692.0 0 13 113 23 692.0 0 23 114 74 692.0 0 74 115 54 692.0 0 54 116 144 692.0 0 144 117 108 692.0 0 108 118 21 692.0 0 21 119 17 692.0 0 17 120 25 692.0 0 25 121 30 692.0 0 30 122 24 692.0 0 24 123 37 692.0 0 37 124 24 692.0 0 24 125 32 692.0 0 32 126 20 692.0 0 20 127 43 692.0 0 43 128 28 692.0 0 28 129 13 692.0 0 13 130 22 692.0 0 22 131 10 692.0 0 10 132 12 692.0 0 12 133 31 692.0 0 31 134 45 692.0 0 45 135 123 692.0 0 123 136 184 692.0 0 184 137 142 692.0 0 142 138 77 692.0 0 77 139 24 692.0 0 24 140 11 692.0 0 11 141 28 692.0 0 28 142 46 692.0 0 46 143 29 692.0 0 29 144 42 692.0 0 42 145 19 692.0 0 19 146 12 692.0 0 12 147 16 692.0 0 16 148 25 692.0 0 25 149 19 692.0 0 19 150 70 692.0 0 70 151 20 692.0 0 20 152 98 692.0 0 98 153 26 692.0 0 26 154 30 692.0 0 30 155 26 692.0 0 26 156 16 692.0 0 16 157 18 692.0 0 18 158 22 692.0 0 22 159 25 692.0 0 25 160 52 692.0 0 52 161 32 692.0 0 32 162 37 692.0 0 37 163 82 692.0 0 82 164 129 692.0 0 129 165 85 692.0 0 85 166 83 692.0 0 83 167 45 692.0 0 45 168 47 692.0 0 47 169 57 692.0 0 57 170 79 692.0 0 79 171 83 692.0 0 83 172 180 692.0 0 180 173 1454 692.0 0 1454 174 1050 692.0 0 1050 175 3789 692.0 0 3789 176 90 692.0 0 90 177 45 692.0 0 45 178 60 692.0 0 60 179 146 692.0 0 146 180 1149 692.0 0 1149 181 378 692.0 0 378 182 583 692.0 0 583 183 38 692.0 0 38 184 55 692.0 0 55 185 77 692.0 0 77 186 94 692.0 0 94 187 65 692.0 0 65 188 104 692.0 0 104 189 212 692.0 0 212 190 494 692.0 0 494 191 6551 692.0 0 6551 192 1471 692.0 0 1471 193 2136 692.0 0 2136 194 151 692.0 0 151 195 18 692.0 0 18 196 13 692.0 0 13 197 29 692.0 0 29 198 45 692.0 0 45 199 65 692.0 0 65 200 30 692.0 0 30 201 26 692.0 0 26 202 232 692.0 0 232 203 356 692.0 0 356 204 243 692.0 0 243 205 137 692.0 0 137 206 38 692.0 0 38 207 31 692.0 0 31 208 220 692.0 0 220 209 232 692.0 0 232 210 286 692.0 0 286 211 73 692.0 0 73 212 26 692.0 0 26 213 25 692.0 0 25 214 18 692.0 0 18 215 39 692.0 0 39 216 32 692.0 0 32 217 44 692.0 0 44 218 90 692.0 0 90 219 27 692.0 0 27 220 41 692.0 0 41 221 16 692.0 0 16 222 11 692.0 0 11 223 12 692.0 0 12 224 22 692.0 0 22 225 26 692.0 0 26 226 26 692.0 0 26 227 39 692.0 0 39 228 18 692.0 0 18 229 48 692.0 0 48 230 24 692.0 0 24 231 53 692.0 0 53 232 29 692.0 0 29 233 31 692.0 0 31 234 62 692.0 0 62 235 45 692.0 0 45 236 31 692.0 0 31 237 29 692.0 0 29 238 20 692.0 0 20 239 15 692.0 0 15 240 37 692.0 0 37 241 23 692.0 0 23 242 48 692.0 0 48 243 34 692.0 0 34 244 39 692.0 0 39 245 29 692.0 0 29 246 41 692.0 0 41 247 49 692.0 0 49 248 68 692.0 0 68 249 48 692.0 0 48 250 45 692.0 0 45 251 153 692.0 0 153 252 251 692.0 0 251 253 316 692.0 0 316 254 435 692.0 0 435 255 67 692.0 0 67 256 30 692.0 0 30 257 52 692.0 0 52 258 155 692.0 0 155 259 617 692.0 0 617 260 812 692.0 0 812 261 712 692.0 0 712 262 240 692.0 0 240 263 142 692.0 0 142 264 264 692.0 0 264 265 288 692.0 0 288 266 141 692.0 0 141 267 78 692.0 0 78 268 59 692.0 0 59 269 25 692.0 0 25 270 70 692.0 0 70 271 132 692.0 0 132 272 116 692.0 0 116 273 115 692.0 0 115 274 99 692.0 0 99 275 73 692.0 0 73 276 66 692.0 0 66 277 105 692.0 0 105 278 147 692.0 0 147 279 173 692.0 0 173 280 155 692.0 0 155 281 144 692.0 0 144 282 180 692.0 0 180 283 404 692.0 0 404 284 4170 692.0 0 4170 285 3448 692.0 0 3448 286 10023 692.0 0 10023 287 102 692.0 0 102 288 79 692.0 0 79 289 69 692.0 0 69 290 47 692.0 0 47 291 60 692.0 0 60 292 48 692.0 0 48 293 40 692.0 0 40 294 55 692.0 0 55 295 101 692.0 0 101 296 125 692.0 0 125 297 290 692.0 0 290 298 102 692.0 0 102 299 48 692.0 0 48 300 40 692.0 0 40 301 126 692.0 0 126 Finished in 1.91 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 47,293 Reads with adapters: 4,820 (10.2%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 4,799 (10.1%) Total basepairs processed: 9,020,401 bp Total written (filtered): 1,304,788 bp (14.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4820 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 8 0.0 3 0 0 4 2 2 19 94 0.0 3 0 78 5 5 6 20 4635 0.0 4 4302 268 48 13 4 21 21 0.0 4 6 13 0 0 2 22 1 0.0 4 0 0 1 23 30 0.0 4 0 0 0 22 8 26 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 1 119 1 0.0 4 0 0 0 0 1 126 1 0.0 4 1 127 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 135 1 0.0 4 1 136 1 0.0 4 0 0 1 151 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 157 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 4,799 Reads with adapters: 1,146 (23.9%) Reads written (passing filters): 4,799 (100.0%) Total basepairs processed: 1,304,788 bp Total written (filtered): 1,292,039 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1146 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 8.6% G: 86.4% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 75.0 0 2 4 2 18.7 0 2 9 172 0.0 0 18 154 10 850 0.0 1 72 778 11 105 0.0 1 9 96 12 4 0.0 1 1 3 13 4 0.0 1 0 4 169 1 0.0 1 1 208 5 0.0 1 5 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.