This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.17 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 54,135 (1.9%) Reads that were too short: 12,890 (0.5%) Reads written (passing filters): 41,245 (1.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 8,123,436 bp (1.0%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 54135 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 313 692.0 0 313 7 208 692.0 0 208 8 5841 692.0 0 5841 9 5189 692.0 0 5189 10 15 692.0 0 15 11 1 692.0 0 1 13 2 692.0 0 2 14 1 692.0 0 1 15 3 692.0 0 3 16 3 692.0 0 3 17 2 692.0 0 2 18 23 692.0 0 23 19 19 692.0 0 19 20 11 692.0 0 11 21 5 692.0 0 5 22 2 692.0 0 2 24 2 692.0 0 2 27 2 692.0 0 2 28 8 692.0 0 8 29 6 692.0 0 6 30 1 692.0 0 1 31 5 692.0 0 5 32 2 692.0 0 2 33 13 692.0 0 13 34 6 692.0 0 6 35 9 692.0 0 9 36 7 692.0 0 7 37 17 692.0 0 17 38 18 692.0 0 18 39 6 692.0 0 6 40 11 692.0 0 11 41 11 692.0 0 11 42 8 692.0 0 8 43 18 692.0 0 18 44 45 692.0 0 45 45 57 692.0 0 57 46 32 692.0 0 32 47 9 692.0 0 9 48 10 692.0 0 10 49 14 692.0 0 14 50 28 692.0 0 28 51 17 692.0 0 17 52 15 692.0 0 15 53 10 692.0 0 10 54 12 692.0 0 12 55 7 692.0 0 7 56 34 692.0 0 34 57 10 692.0 0 10 58 13 692.0 0 13 59 51 692.0 0 51 60 49 692.0 0 49 61 9 692.0 0 9 62 17 692.0 0 17 63 8 692.0 0 8 64 12 692.0 0 12 65 22 692.0 0 22 66 35 692.0 0 35 67 19 692.0 0 19 68 42 692.0 0 42 69 26 692.0 0 26 70 26 692.0 0 26 71 39 692.0 0 39 72 68 692.0 0 68 73 45 692.0 0 45 74 77 692.0 0 77 75 116 692.0 0 116 76 229 692.0 0 229 77 2457 692.0 0 2457 78 2684 692.0 0 2684 79 4445 692.0 0 4445 80 45 692.0 0 45 81 8 692.0 0 8 82 50 692.0 0 50 83 10 692.0 0 10 84 11 692.0 0 11 85 6 692.0 0 6 86 11 692.0 0 11 87 11 692.0 0 11 88 3 692.0 0 3 89 4 692.0 0 4 90 10 692.0 0 10 91 16 692.0 0 16 92 17 692.0 0 17 93 18 692.0 0 18 94 43 692.0 0 43 95 34 692.0 0 34 96 18 692.0 0 18 97 8 692.0 0 8 98 6 692.0 0 6 99 8 692.0 0 8 100 5 692.0 0 5 101 11 692.0 0 11 102 8 692.0 0 8 103 38 692.0 0 38 104 26 692.0 0 26 105 20 692.0 0 20 106 12 692.0 0 12 107 13 692.0 0 13 108 30 692.0 0 30 109 39 692.0 0 39 110 5 692.0 0 5 111 13 692.0 0 13 112 11 692.0 0 11 113 10 692.0 0 10 114 77 692.0 0 77 115 34 692.0 0 34 116 166 692.0 0 166 117 91 692.0 0 91 118 22 692.0 0 22 119 16 692.0 0 16 120 18 692.0 0 18 121 12 692.0 0 12 122 13 692.0 0 13 123 33 692.0 0 33 124 17 692.0 0 17 125 10 692.0 0 10 126 16 692.0 0 16 127 17 692.0 0 17 128 3 692.0 0 3 129 6 692.0 0 6 130 14 692.0 0 14 131 16 692.0 0 16 132 9 692.0 0 9 133 22 692.0 0 22 134 25 692.0 0 25 135 51 692.0 0 51 136 129 692.0 0 129 137 81 692.0 0 81 138 67 692.0 0 67 139 23 692.0 0 23 140 14 692.0 0 14 141 11 692.0 0 11 142 16 692.0 0 16 143 19 692.0 0 19 144 33 692.0 0 33 145 5 692.0 0 5 146 10 692.0 0 10 147 14 692.0 0 14 148 13 692.0 0 13 149 10 692.0 0 10 150 38 692.0 0 38 151 19 692.0 0 19 152 99 692.0 0 99 153 11 692.0 0 11 154 15 692.0 0 15 155 16 692.0 0 16 156 6 692.0 0 6 157 20 692.0 0 20 158 10 692.0 0 10 159 14 692.0 0 14 160 22 692.0 0 22 161 17 692.0 0 17 162 32 692.0 0 32 163 52 692.0 0 52 164 64 692.0 0 64 165 42 692.0 0 42 166 35 692.0 0 35 167 25 692.0 0 25 168 25 692.0 0 25 169 36 692.0 0 36 170 22 692.0 0 22 171 37 692.0 0 37 172 71 692.0 0 71 173 724 692.0 0 724 174 513 692.0 0 513 175 1620 692.0 0 1620 176 76 692.0 0 76 177 30 692.0 0 30 178 57 692.0 0 57 179 75 692.0 0 75 180 638 692.0 0 638 181 195 692.0 0 195 182 438 692.0 0 438 183 17 692.0 0 17 184 34 692.0 0 34 185 38 692.0 0 38 186 61 692.0 0 61 187 56 692.0 0 56 188 65 692.0 0 65 189 188 692.0 0 188 190 409 692.0 0 409 191 7262 692.0 0 7262 192 1285 692.0 0 1285 193 2222 692.0 0 2222 194 129 692.0 0 129 195 10 692.0 0 10 196 5 692.0 0 5 197 15 692.0 0 15 198 12 692.0 0 12 199 27 692.0 0 27 200 16 692.0 0 16 201 12 692.0 0 12 202 153 692.0 0 153 203 154 692.0 0 154 204 118 692.0 0 118 205 60 692.0 0 60 206 28 692.0 0 28 207 15 692.0 0 15 208 118 692.0 0 118 209 34 692.0 0 34 210 56 692.0 0 56 211 19 692.0 0 19 212 16 692.0 0 16 213 12 692.0 0 12 214 8 692.0 0 8 215 40 692.0 0 40 216 11 692.0 0 11 217 12 692.0 0 12 218 25 692.0 0 25 219 11 692.0 0 11 220 13 692.0 0 13 221 9 692.0 0 9 222 2 692.0 0 2 223 8 692.0 0 8 224 13 692.0 0 13 225 30 692.0 0 30 226 10 692.0 0 10 227 9 692.0 0 9 228 12 692.0 0 12 229 19 692.0 0 19 230 15 692.0 0 15 231 71 692.0 0 71 232 15 692.0 0 15 233 14 692.0 0 14 234 21 692.0 0 21 235 23 692.0 0 23 236 20 692.0 0 20 237 13 692.0 0 13 238 18 692.0 0 18 239 15 692.0 0 15 240 20 692.0 0 20 241 19 692.0 0 19 242 21 692.0 0 21 243 12 692.0 0 12 244 17 692.0 0 17 245 14 692.0 0 14 246 15 692.0 0 15 247 24 692.0 0 24 248 30 692.0 0 30 249 20 692.0 0 20 250 21 692.0 0 21 251 57 692.0 0 57 252 121 692.0 0 121 253 133 692.0 0 133 254 179 692.0 0 179 255 25 692.0 0 25 256 26 692.0 0 26 257 45 692.0 0 45 258 130 692.0 0 130 259 713 692.0 0 713 260 412 692.0 0 412 261 510 692.0 0 510 262 111 692.0 0 111 263 58 692.0 0 58 264 113 692.0 0 113 265 154 692.0 0 154 266 69 692.0 0 69 267 40 692.0 0 40 268 15 692.0 0 15 269 9 692.0 0 9 270 25 692.0 0 25 271 36 692.0 0 36 272 36 692.0 0 36 273 27 692.0 0 27 274 35 692.0 0 35 275 30 692.0 0 30 276 20 692.0 0 20 277 34 692.0 0 34 278 54 692.0 0 54 279 47 692.0 0 47 280 53 692.0 0 53 281 29 692.0 0 29 282 52 692.0 0 52 283 127 692.0 0 127 284 1744 692.0 0 1744 285 1372 692.0 0 1372 286 4192 692.0 0 4192 287 40 692.0 0 40 288 105 692.0 0 105 289 52 692.0 0 52 290 21 692.0 0 21 291 36 692.0 0 36 292 21 692.0 0 21 293 11 692.0 0 11 294 13 692.0 0 13 295 40 692.0 0 40 296 44 692.0 0 44 297 118 692.0 0 118 298 21 692.0 0 21 299 6 692.0 0 6 300 10 692.0 0 10 301 66 692.0 0 66 Finished in 1.60 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 41,245 Reads with adapters: 5,657 (13.7%) Reads that were too short: 55 (0.1%) Reads written (passing filters): 5,602 (13.6%) Total basepairs processed: 8,123,436 bp Total written (filtered): 1,533,449 bp (18.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5657 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 40 0.0 3 0 38 0 2 17 5232 0.0 3 5139 85 6 2 18 62 0.0 3 1 9 3 49 19 9 0.0 3 0 3 2 4 20 235 0.0 3 218 4 0 13 21 13 0.0 3 0 1 1 11 27 1 0.0 3 0 0 1 30 1 0.0 3 0 0 0 1 35 1 0.0 3 1 39 4 0.0 3 0 0 0 4 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 92 1 0.0 3 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 119 6 0.0 3 0 0 0 6 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 1 138 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 217 17 0.0 3 0 0 0 17 224 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 5,602 Reads with adapters: 24 (0.4%) Reads written (passing filters): 5,602 (100.0%) Total basepairs processed: 1,533,449 bp Total written (filtered): 1,533,369 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 24 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.3% C: 12.5% G: 12.5% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 19 87.5 0 19 4 4 21.9 0 4 7 1 0.3 0 1