This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.60 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 156,680 (5.5%) Reads that were too short: 13,394 (0.5%) Reads written (passing filters): 143,286 (5.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 40,904,744 bp (4.8%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 156680 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1333 692.0 0 1333 7 73826 692.0 0 73826 8 57360 692.0 0 57360 9 57 692.0 0 57 10 3 692.0 0 3 11 4 692.0 0 4 12 7 692.0 0 7 13 16 692.0 0 16 14 11 692.0 0 11 15 11 692.0 0 11 16 7 692.0 0 7 17 13 692.0 0 13 18 69 692.0 0 69 19 35 692.0 0 35 20 27 692.0 0 27 21 6 692.0 0 6 22 7 692.0 0 7 23 25 692.0 0 25 24 5 692.0 0 5 25 5 692.0 0 5 26 29 692.0 0 29 27 34 692.0 0 34 28 42 692.0 0 42 29 29 692.0 0 29 30 25 692.0 0 25 31 7 692.0 0 7 32 10 692.0 0 10 33 44 692.0 0 44 34 26 692.0 0 26 35 32 692.0 0 32 36 107 692.0 0 107 37 107 692.0 0 107 38 525 692.0 0 525 39 15 692.0 0 15 40 17 692.0 0 17 41 12 692.0 0 12 42 10 692.0 0 10 43 154 692.0 0 154 44 177 692.0 0 177 45 652 692.0 0 652 46 25 692.0 0 25 47 8 692.0 0 8 48 7 692.0 0 7 49 21 692.0 0 21 50 11 692.0 0 11 51 10 692.0 0 10 52 11 692.0 0 11 53 13 692.0 0 13 54 7 692.0 0 7 55 12 692.0 0 12 56 18 692.0 0 18 57 66 692.0 0 66 58 12 692.0 0 12 59 19 692.0 0 19 60 8 692.0 0 8 61 14 692.0 0 14 62 15 692.0 0 15 63 12 692.0 0 12 64 19 692.0 0 19 65 12 692.0 0 12 66 17 692.0 0 17 67 8 692.0 0 8 68 19 692.0 0 19 69 8 692.0 0 8 70 19 692.0 0 19 71 70 692.0 0 70 72 17 692.0 0 17 73 47 692.0 0 47 74 22 692.0 0 22 75 28 692.0 0 28 76 15 692.0 0 15 77 15 692.0 0 15 78 18 692.0 0 18 79 43 692.0 0 43 80 39 692.0 0 39 81 18 692.0 0 18 82 35 692.0 0 35 83 41 692.0 0 41 84 18 692.0 0 18 85 30 692.0 0 30 86 28 692.0 0 28 87 23 692.0 0 23 88 12 692.0 0 12 89 33 692.0 0 33 90 25 692.0 0 25 91 30 692.0 0 30 92 18 692.0 0 18 93 24 692.0 0 24 94 44 692.0 0 44 95 24 692.0 0 24 96 50 692.0 0 50 97 65 692.0 0 65 98 16 692.0 0 16 99 14 692.0 0 14 100 3 692.0 0 3 101 7 692.0 0 7 102 9 692.0 0 9 103 15 692.0 0 15 104 20 692.0 0 20 105 24 692.0 0 24 106 22 692.0 0 22 107 25 692.0 0 25 108 30 692.0 0 30 109 25 692.0 0 25 110 19 692.0 0 19 111 25 692.0 0 25 112 33 692.0 0 33 113 41 692.0 0 41 114 28 692.0 0 28 115 30 692.0 0 30 116 26 692.0 0 26 117 23 692.0 0 23 118 16 692.0 0 16 119 20 692.0 0 20 120 47 692.0 0 47 121 45 692.0 0 45 122 55 692.0 0 55 123 27 692.0 0 27 124 38 692.0 0 38 125 29 692.0 0 29 126 71 692.0 0 71 127 63 692.0 0 63 128 196 692.0 0 196 129 63 692.0 0 63 130 68 692.0 0 68 131 57 692.0 0 57 132 41 692.0 0 41 133 132 692.0 0 132 134 141 692.0 0 141 135 1182 692.0 0 1182 136 238 692.0 0 238 137 438 692.0 0 438 138 49 692.0 0 49 139 56 692.0 0 56 140 98 692.0 0 98 141 148 692.0 0 148 142 132 692.0 0 132 143 63 692.0 0 63 144 45 692.0 0 45 145 38 692.0 0 38 146 57 692.0 0 57 147 96 692.0 0 96 148 201 692.0 0 201 149 376 692.0 0 376 150 233 692.0 0 233 151 55 692.0 0 55 152 27 692.0 0 27 153 59 692.0 0 59 154 33 692.0 0 33 155 46 692.0 0 46 156 56 692.0 0 56 157 30 692.0 0 30 158 31 692.0 0 31 159 23 692.0 0 23 160 35 692.0 0 35 161 50 692.0 0 50 162 30 692.0 0 30 163 45 692.0 0 45 164 49 692.0 0 49 165 37 692.0 0 37 166 38 692.0 0 38 167 63 692.0 0 63 168 37 692.0 0 37 169 30 692.0 0 30 170 26 692.0 0 26 171 28 692.0 0 28 172 30 692.0 0 30 173 35 692.0 0 35 174 39 692.0 0 39 175 53 692.0 0 53 176 48 692.0 0 48 177 38 692.0 0 38 178 37 692.0 0 37 179 24 692.0 0 24 180 72 692.0 0 72 181 42 692.0 0 42 182 77 692.0 0 77 183 29 692.0 0 29 184 40 692.0 0 40 185 46 692.0 0 46 186 49 692.0 0 49 187 53 692.0 0 53 188 38 692.0 0 38 189 35 692.0 0 35 190 54 692.0 0 54 191 51 692.0 0 51 192 40 692.0 0 40 193 52 692.0 0 52 194 66 692.0 0 66 195 66 692.0 0 66 196 45 692.0 0 45 197 37 692.0 0 37 198 31 692.0 0 31 199 36 692.0 0 36 200 48 692.0 0 48 201 49 692.0 0 49 202 64 692.0 0 64 203 45 692.0 0 45 204 26 692.0 0 26 205 30 692.0 0 30 206 40 692.0 0 40 207 80 692.0 0 80 208 130 692.0 0 130 209 277 692.0 0 277 210 96 692.0 0 96 211 46 692.0 0 46 212 118 692.0 0 118 213 100 692.0 0 100 214 169 692.0 0 169 215 203 692.0 0 203 216 107 692.0 0 107 217 62 692.0 0 62 218 37 692.0 0 37 219 81 692.0 0 81 220 79 692.0 0 79 221 86 692.0 0 86 222 80 692.0 0 80 223 42 692.0 0 42 224 45 692.0 0 45 225 48 692.0 0 48 226 69 692.0 0 69 227 50 692.0 0 50 228 86 692.0 0 86 229 72 692.0 0 72 230 133 692.0 0 133 231 69 692.0 0 69 232 86 692.0 0 86 233 195 692.0 0 195 234 153 692.0 0 153 235 359 692.0 0 359 236 85 692.0 0 85 237 46 692.0 0 46 238 68 692.0 0 68 239 34 692.0 0 34 240 53 692.0 0 53 241 44 692.0 0 44 242 61 692.0 0 61 243 80 692.0 0 80 244 71 692.0 0 71 245 51 692.0 0 51 246 63 692.0 0 63 247 33 692.0 0 33 248 51 692.0 0 51 249 47 692.0 0 47 250 46 692.0 0 46 251 42 692.0 0 42 252 49 692.0 0 49 253 48 692.0 0 48 254 52 692.0 0 52 255 65 692.0 0 65 256 71 692.0 0 71 257 51 692.0 0 51 258 63 692.0 0 63 259 71 692.0 0 71 260 88 692.0 0 88 261 66 692.0 0 66 262 84 692.0 0 84 263 56 692.0 0 56 264 82 692.0 0 82 265 64 692.0 0 64 266 57 692.0 0 57 267 90 692.0 0 90 268 171 692.0 0 171 269 673 692.0 0 673 270 301 692.0 0 301 271 601 692.0 0 601 272 123 692.0 0 123 273 136 692.0 0 136 274 240 692.0 0 240 275 120 692.0 0 120 276 140 692.0 0 140 277 98 692.0 0 98 278 97 692.0 0 97 279 97 692.0 0 97 280 174 692.0 0 174 281 252 692.0 0 252 282 204 692.0 0 204 283 136 692.0 0 136 284 67 692.0 0 67 285 81 692.0 0 81 286 65 692.0 0 65 287 85 692.0 0 85 288 131 692.0 0 131 289 80 692.0 0 80 290 124 692.0 0 124 291 116 692.0 0 116 292 130 692.0 0 130 293 280 692.0 0 280 294 542 692.0 0 542 295 544 692.0 0 544 296 1159 692.0 0 1159 297 455 692.0 0 455 298 246 692.0 0 246 299 71 692.0 0 71 300 117 692.0 0 117 301 243 692.0 0 243 Finished in 7.37 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 143,286 Reads with adapters: 60,384 (42.1%) Reads that were too short: 55 (0.0%) Reads written (passing filters): 60,329 (42.1%) Total basepairs processed: 40,904,744 bp Total written (filtered): 16,435,847 bp (40.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 60384 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 1 3 18 37 0.0 3 0 0 19 12 6 19 907 0.0 3 0 701 115 63 28 20 58690 0.0 4 54948 3017 538 151 36 21 126 0.0 4 15 98 8 3 2 22 13 0.0 4 1 0 8 1 3 23 208 0.0 4 0 0 1 128 79 24 4 0.0 4 0 0 0 0 4 25 2 0.0 4 1 0 0 0 1 28 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 311 0.0 4 0 0 0 0 311 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 1 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 0 7 154 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 1 169 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 1 184 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 194 2 0.0 4 0 0 0 0 2 195 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 233 3 0.0 4 0 0 0 0 3 234 6 0.0 4 0 0 0 0 6 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 293 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.16 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 60,329 Reads with adapters: 11,527 (19.1%) Reads written (passing filters): 60,329 (100.0%) Total basepairs processed: 16,435,847 bp Total written (filtered): 16,285,586 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11527 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 8.3% G: 85.3% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 69 942.6 0 69 4 13 235.7 0 13 8 1 0.9 0 1 9 35 0.2 0 1 34 10 1949 0.1 1 148 1801 11 9192 0.0 1 420 8772 12 99 0.0 1 2 97 13 25 0.0 1 0 25 14 11 0.0 1 0 11 151 6 0.0 1 5 1 153 1 0.0 1 1 171 1 0.0 1 1 209 112 0.0 1 95 17 210 8 0.0 1 7 1 211 3 0.0 1 2 1 229 1 0.0 1 1 230 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.