This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.01 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 150,699 (5.3%) Reads that were too short: 7,091 (0.3%) Reads written (passing filters): 143,608 (5.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 41,357,999 bp (4.8%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 150699 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 692.0 0 1019 7 63091 692.0 0 63091 8 72280 692.0 0 72280 9 53 692.0 0 53 10 13 692.0 0 13 11 16 692.0 0 16 12 8 692.0 0 8 13 8 692.0 0 8 14 15 692.0 0 15 15 13 692.0 0 13 16 15 692.0 0 15 17 9 692.0 0 9 18 27 692.0 0 27 19 21 692.0 0 21 20 28 692.0 0 28 21 14 692.0 0 14 22 13 692.0 0 13 23 12 692.0 0 12 24 9 692.0 0 9 25 7 692.0 0 7 26 13 692.0 0 13 27 15 692.0 0 15 28 13 692.0 0 13 29 17 692.0 0 17 30 11 692.0 0 11 31 7 692.0 0 7 32 18 692.0 0 18 33 9 692.0 0 9 34 20 692.0 0 20 35 22 692.0 0 22 36 73 692.0 0 73 37 76 692.0 0 76 38 174 692.0 0 174 39 9 692.0 0 9 40 15 692.0 0 15 41 10 692.0 0 10 42 18 692.0 0 18 43 42 692.0 0 42 44 61 692.0 0 61 45 162 692.0 0 162 46 10 692.0 0 10 47 18 692.0 0 18 48 17 692.0 0 17 49 30 692.0 0 30 50 24 692.0 0 24 51 17 692.0 0 17 52 17 692.0 0 17 53 21 692.0 0 21 54 13 692.0 0 13 55 22 692.0 0 22 56 32 692.0 0 32 57 47 692.0 0 47 58 27 692.0 0 27 59 35 692.0 0 35 60 9 692.0 0 9 61 22 692.0 0 22 62 24 692.0 0 24 63 22 692.0 0 22 64 17 692.0 0 17 65 13 692.0 0 13 66 18 692.0 0 18 67 10 692.0 0 10 68 13 692.0 0 13 69 19 692.0 0 19 70 24 692.0 0 24 71 71 692.0 0 71 72 6 692.0 0 6 73 31 692.0 0 31 74 12 692.0 0 12 75 13 692.0 0 13 76 17 692.0 0 17 77 25 692.0 0 25 78 24 692.0 0 24 79 42 692.0 0 42 80 16 692.0 0 16 81 10 692.0 0 10 82 21 692.0 0 21 83 13 692.0 0 13 84 20 692.0 0 20 85 17 692.0 0 17 86 19 692.0 0 19 87 44 692.0 0 44 88 35 692.0 0 35 89 40 692.0 0 40 90 26 692.0 0 26 91 27 692.0 0 27 92 18 692.0 0 18 93 29 692.0 0 29 94 49 692.0 0 49 95 32 692.0 0 32 96 35 692.0 0 35 97 40 692.0 0 40 98 27 692.0 0 27 99 21 692.0 0 21 100 9 692.0 0 9 101 16 692.0 0 16 102 10 692.0 0 10 103 19 692.0 0 19 104 25 692.0 0 25 105 21 692.0 0 21 106 18 692.0 0 18 107 24 692.0 0 24 108 20 692.0 0 20 109 21 692.0 0 21 110 14 692.0 0 14 111 11 692.0 0 11 112 30 692.0 0 30 113 26 692.0 0 26 114 16 692.0 0 16 115 30 692.0 0 30 116 13 692.0 0 13 117 21 692.0 0 21 118 27 692.0 0 27 119 21 692.0 0 21 120 37 692.0 0 37 121 36 692.0 0 36 122 38 692.0 0 38 123 16 692.0 0 16 124 13 692.0 0 13 125 25 692.0 0 25 126 46 692.0 0 46 127 27 692.0 0 27 128 68 692.0 0 68 129 53 692.0 0 53 130 43 692.0 0 43 131 45 692.0 0 45 132 37 692.0 0 37 133 135 692.0 0 135 134 166 692.0 0 166 135 845 692.0 0 845 136 180 692.0 0 180 137 303 692.0 0 303 138 32 692.0 0 32 139 30 692.0 0 30 140 80 692.0 0 80 141 111 692.0 0 111 142 82 692.0 0 82 143 53 692.0 0 53 144 31 692.0 0 31 145 26 692.0 0 26 146 35 692.0 0 35 147 53 692.0 0 53 148 83 692.0 0 83 149 199 692.0 0 199 150 99 692.0 0 99 151 39 692.0 0 39 152 21 692.0 0 21 153 58 692.0 0 58 154 27 692.0 0 27 155 31 692.0 0 31 156 29 692.0 0 29 157 20 692.0 0 20 158 14 692.0 0 14 159 20 692.0 0 20 160 26 692.0 0 26 161 21 692.0 0 21 162 20 692.0 0 20 163 24 692.0 0 24 164 28 692.0 0 28 165 27 692.0 0 27 166 28 692.0 0 28 167 32 692.0 0 32 168 17 692.0 0 17 169 20 692.0 0 20 170 21 692.0 0 21 171 22 692.0 0 22 172 20 692.0 0 20 173 35 692.0 0 35 174 33 692.0 0 33 175 40 692.0 0 40 176 20 692.0 0 20 177 22 692.0 0 22 178 20 692.0 0 20 179 23 692.0 0 23 180 40 692.0 0 40 181 34 692.0 0 34 182 44 692.0 0 44 183 19 692.0 0 19 184 28 692.0 0 28 185 29 692.0 0 29 186 34 692.0 0 34 187 27 692.0 0 27 188 26 692.0 0 26 189 25 692.0 0 25 190 29 692.0 0 29 191 33 692.0 0 33 192 20 692.0 0 20 193 34 692.0 0 34 194 39 692.0 0 39 195 62 692.0 0 62 196 23 692.0 0 23 197 24 692.0 0 24 198 20 692.0 0 20 199 22 692.0 0 22 200 25 692.0 0 25 201 37 692.0 0 37 202 64 692.0 0 64 203 42 692.0 0 42 204 19 692.0 0 19 205 33 692.0 0 33 206 46 692.0 0 46 207 57 692.0 0 57 208 56 692.0 0 56 209 126 692.0 0 126 210 49 692.0 0 49 211 39 692.0 0 39 212 96 692.0 0 96 213 50 692.0 0 50 214 50 692.0 0 50 215 77 692.0 0 77 216 73 692.0 0 73 217 28 692.0 0 28 218 25 692.0 0 25 219 36 692.0 0 36 220 59 692.0 0 59 221 52 692.0 0 52 222 38 692.0 0 38 223 34 692.0 0 34 224 28 692.0 0 28 225 30 692.0 0 30 226 32 692.0 0 32 227 50 692.0 0 50 228 48 692.0 0 48 229 44 692.0 0 44 230 84 692.0 0 84 231 37 692.0 0 37 232 48 692.0 0 48 233 68 692.0 0 68 234 58 692.0 0 58 235 101 692.0 0 101 236 48 692.0 0 48 237 38 692.0 0 38 238 39 692.0 0 39 239 24 692.0 0 24 240 39 692.0 0 39 241 29 692.0 0 29 242 38 692.0 0 38 243 45 692.0 0 45 244 49 692.0 0 49 245 36 692.0 0 36 246 26 692.0 0 26 247 30 692.0 0 30 248 41 692.0 0 41 249 27 692.0 0 27 250 23 692.0 0 23 251 29 692.0 0 29 252 54 692.0 0 54 253 42 692.0 0 42 254 29 692.0 0 29 255 33 692.0 0 33 256 36 692.0 0 36 257 33 692.0 0 33 258 49 692.0 0 49 259 37 692.0 0 37 260 39 692.0 0 39 261 47 692.0 0 47 262 38 692.0 0 38 263 50 692.0 0 50 264 40 692.0 0 40 265 35 692.0 0 35 266 34 692.0 0 34 267 56 692.0 0 56 268 52 692.0 0 52 269 257 692.0 0 257 270 114 692.0 0 114 271 256 692.0 0 256 272 57 692.0 0 57 273 66 692.0 0 66 274 80 692.0 0 80 275 65 692.0 0 65 276 66 692.0 0 66 277 49 692.0 0 49 278 59 692.0 0 59 279 54 692.0 0 54 280 82 692.0 0 82 281 148 692.0 0 148 282 130 692.0 0 130 283 65 692.0 0 65 284 44 692.0 0 44 285 50 692.0 0 50 286 58 692.0 0 58 287 48 692.0 0 48 288 77 692.0 0 77 289 81 692.0 0 81 290 59 692.0 0 59 291 57 692.0 0 57 292 80 692.0 0 80 293 113 692.0 0 113 294 335 692.0 0 335 295 344 692.0 0 344 296 538 692.0 0 538 297 232 692.0 0 232 298 127 692.0 0 127 299 48 692.0 0 48 300 57 692.0 0 57 301 153 692.0 0 153 Finished in 6.52 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 143,608 Reads with adapters: 61,273 (42.7%) Reads that were too short: 64 (0.0%) Reads written (passing filters): 61,209 (42.6%) Total basepairs processed: 41,357,999 bp Total written (filtered): 16,950,847 bp (41.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 61273 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 16 0.0 3 0 0 15 1 16 430 0.0 3 0 419 11 17 60483 0.0 3 59198 1241 39 5 18 120 0.0 3 13 102 4 1 19 34 0.0 3 2 0 27 5 20 97 0.0 3 0 0 0 97 22 1 0.0 3 0 0 1 23 2 0.0 3 2 25 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 1 1 148 2 0.0 3 1 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 217 39 0.0 3 0 0 0 39 218 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.14 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 61,209 Reads with adapters: 126 (0.2%) Reads written (passing filters): 61,209 (100.0%) Total basepairs processed: 16,950,847 bp Total written (filtered): 16,950,434 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 126 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.4% C: 41.3% G: 27.0% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 103 956.4 0 103 4 16 239.1 0 16 5 2 59.8 0 2 6 5 14.9 0 5