This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.04 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 144,948 (5.1%) Reads that were too short: 16,040 (0.6%) Reads written (passing filters): 128,908 (4.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 35,806,522 bp (4.2%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 144948 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3271 692.0 0 3271 7 44301 692.0 0 44301 8 1193 692.0 0 1193 9 63126 692.0 0 63126 10 54 692.0 0 54 11 33 692.0 0 33 12 30 692.0 0 30 13 36 692.0 0 36 14 47 692.0 0 47 15 33 692.0 0 33 16 34 692.0 0 34 17 62 692.0 0 62 18 33 692.0 0 33 19 59 692.0 0 59 20 23 692.0 0 23 21 51 692.0 0 51 22 34 692.0 0 34 23 41 692.0 0 41 24 37 692.0 0 37 25 17 692.0 0 17 26 40 692.0 0 40 27 36 692.0 0 36 28 36 692.0 0 36 29 40 692.0 0 40 30 38 692.0 0 38 31 45 692.0 0 45 32 41 692.0 0 41 33 37 692.0 0 37 34 31 692.0 0 31 35 35 692.0 0 35 36 27 692.0 0 27 37 37 692.0 0 37 38 63 692.0 0 63 39 39 692.0 0 39 40 37 692.0 0 37 41 48 692.0 0 48 42 257 692.0 0 257 43 125 692.0 0 125 44 491 692.0 0 491 45 29 692.0 0 29 46 26 692.0 0 26 47 36 692.0 0 36 48 61 692.0 0 61 49 29 692.0 0 29 50 66 692.0 0 66 51 40 692.0 0 40 52 34 692.0 0 34 53 29 692.0 0 29 54 30 692.0 0 30 55 39 692.0 0 39 56 41 692.0 0 41 57 27 692.0 0 27 58 42 692.0 0 42 59 45 692.0 0 45 60 36 692.0 0 36 61 31 692.0 0 31 62 31 692.0 0 31 63 31 692.0 0 31 64 31 692.0 0 31 65 30 692.0 0 30 66 58 692.0 0 58 67 77 692.0 0 77 68 30 692.0 0 30 69 39 692.0 0 39 70 31 692.0 0 31 71 34 692.0 0 34 72 41 692.0 0 41 73 46 692.0 0 46 74 46 692.0 0 46 75 86 692.0 0 86 76 68 692.0 0 68 77 93 692.0 0 93 78 24 692.0 0 24 79 82 692.0 0 82 80 47 692.0 0 47 81 114 692.0 0 114 82 32 692.0 0 32 83 28 692.0 0 28 84 31 692.0 0 31 85 30 692.0 0 30 86 32 692.0 0 32 87 30 692.0 0 30 88 44 692.0 0 44 89 36 692.0 0 36 90 53 692.0 0 53 91 54 692.0 0 54 92 445 692.0 0 445 93 402 692.0 0 402 94 738 692.0 0 738 95 34 692.0 0 34 96 41 692.0 0 41 97 35 692.0 0 35 98 33 692.0 0 33 99 36 692.0 0 36 100 32 692.0 0 32 101 45 692.0 0 45 102 28 692.0 0 28 103 22 692.0 0 22 104 32 692.0 0 32 105 25 692.0 0 25 106 34 692.0 0 34 107 36 692.0 0 36 108 48 692.0 0 48 109 50 692.0 0 50 110 52 692.0 0 52 111 35 692.0 0 35 112 50 692.0 0 50 113 35 692.0 0 35 114 47 692.0 0 47 115 47 692.0 0 47 116 52 692.0 0 52 117 69 692.0 0 69 118 80 692.0 0 80 119 86 692.0 0 86 120 75 692.0 0 75 121 79 692.0 0 79 122 83 692.0 0 83 123 1706 692.0 0 1706 124 266 692.0 0 266 125 202 692.0 0 202 126 59 692.0 0 59 127 67 692.0 0 67 128 123 692.0 0 123 129 242 692.0 0 242 130 771 692.0 0 771 131 141 692.0 0 141 132 434 692.0 0 434 133 35 692.0 0 35 134 40 692.0 0 40 135 25 692.0 0 25 136 35 692.0 0 35 137 31 692.0 0 31 138 35 692.0 0 35 139 30 692.0 0 30 140 38 692.0 0 38 141 38 692.0 0 38 142 34 692.0 0 34 143 34 692.0 0 34 144 45 692.0 0 45 145 44 692.0 0 44 146 46 692.0 0 46 147 77 692.0 0 77 148 28 692.0 0 28 149 24 692.0 0 24 150 22 692.0 0 22 151 30 692.0 0 30 152 23 692.0 0 23 153 26 692.0 0 26 154 27 692.0 0 27 155 31 692.0 0 31 156 34 692.0 0 34 157 30 692.0 0 30 158 57 692.0 0 57 159 36 692.0 0 36 160 51 692.0 0 51 161 39 692.0 0 39 162 61 692.0 0 61 163 25 692.0 0 25 164 40 692.0 0 40 165 49 692.0 0 49 166 37 692.0 0 37 167 73 692.0 0 73 168 60 692.0 0 60 169 33 692.0 0 33 170 34 692.0 0 34 171 24 692.0 0 24 172 30 692.0 0 30 173 77 692.0 0 77 174 88 692.0 0 88 175 119 692.0 0 119 176 39 692.0 0 39 177 74 692.0 0 74 178 257 692.0 0 257 179 220 692.0 0 220 180 253 692.0 0 253 181 111 692.0 0 111 182 58 692.0 0 58 183 37 692.0 0 37 184 84 692.0 0 84 185 63 692.0 0 63 186 114 692.0 0 114 187 40 692.0 0 40 188 49 692.0 0 49 189 38 692.0 0 38 190 33 692.0 0 33 191 54 692.0 0 54 192 76 692.0 0 76 193 86 692.0 0 86 194 90 692.0 0 90 195 146 692.0 0 146 196 179 692.0 0 179 197 104 692.0 0 104 198 401 692.0 0 401 199 381 692.0 0 381 200 775 692.0 0 775 201 366 692.0 0 366 202 154 692.0 0 154 203 109 692.0 0 109 204 68 692.0 0 68 205 174 692.0 0 174 206 86 692.0 0 86 207 171 692.0 0 171 208 88 692.0 0 88 209 73 692.0 0 73 210 68 692.0 0 68 211 57 692.0 0 57 212 55 692.0 0 55 213 43 692.0 0 43 214 95 692.0 0 95 215 89 692.0 0 89 216 80 692.0 0 80 217 82 692.0 0 82 218 106 692.0 0 106 219 345 692.0 0 345 220 264 692.0 0 264 221 607 692.0 0 607 222 504 692.0 0 504 223 179 692.0 0 179 224 322 692.0 0 322 225 64 692.0 0 64 226 70 692.0 0 70 227 54 692.0 0 54 228 133 692.0 0 133 229 83 692.0 0 83 230 154 692.0 0 154 231 48 692.0 0 48 232 44 692.0 0 44 233 51 692.0 0 51 234 46 692.0 0 46 235 56 692.0 0 56 236 70 692.0 0 70 237 88 692.0 0 88 238 253 692.0 0 253 239 359 692.0 0 359 240 2335 692.0 0 2335 241 929 692.0 0 929 242 1211 692.0 0 1211 243 61 692.0 0 61 244 51 692.0 0 51 245 30 692.0 0 30 246 79 692.0 0 79 247 74 692.0 0 74 248 111 692.0 0 111 249 82 692.0 0 82 250 237 692.0 0 237 251 250 692.0 0 250 252 487 692.0 0 487 253 114 692.0 0 114 254 58 692.0 0 58 255 57 692.0 0 57 256 71 692.0 0 71 257 66 692.0 0 66 258 69 692.0 0 69 259 38 692.0 0 38 260 75 692.0 0 75 261 142 692.0 0 142 262 125 692.0 0 125 263 377 692.0 0 377 264 34 692.0 0 34 265 46 692.0 0 46 266 35 692.0 0 35 267 67 692.0 0 67 268 34 692.0 0 34 269 54 692.0 0 54 270 70 692.0 0 70 271 63 692.0 0 63 272 70 692.0 0 70 273 44 692.0 0 44 274 49 692.0 0 49 275 58 692.0 0 58 276 52 692.0 0 52 277 88 692.0 0 88 278 105 692.0 0 105 279 92 692.0 0 92 280 73 692.0 0 73 281 70 692.0 0 70 282 69 692.0 0 69 283 79 692.0 0 79 284 68 692.0 0 68 285 56 692.0 0 56 286 103 692.0 0 103 287 139 692.0 0 139 288 280 692.0 0 280 289 221 692.0 0 221 290 301 692.0 0 301 291 244 692.0 0 244 292 152 692.0 0 152 293 71 692.0 0 71 294 69 692.0 0 69 295 68 692.0 0 68 296 123 692.0 0 123 297 78 692.0 0 78 298 54 692.0 0 54 299 87 692.0 0 87 300 100 692.0 0 100 301 53 692.0 0 53 Finished in 6.83 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 128,908 Reads with adapters: 46,624 (36.2%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 46,579 (36.1%) Total basepairs processed: 35,806,522 bp Total written (filtered): 12,759,688 bp (35.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 46624 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 28 0.0 3 0 0 15 9 4 19 598 0.0 3 0 530 51 12 5 20 45722 0.0 4 43321 1991 306 79 25 21 68 0.0 4 7 54 6 1 22 6 0.0 4 0 0 1 2 3 23 95 0.0 4 0 0 1 70 24 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 28 5 0.0 4 0 0 0 0 5 29 2 0.0 4 0 1 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 86 3 0.0 4 0 0 0 0 3 91 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 1 124 1 0.0 4 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 27 0.0 4 0 0 0 0 27 130 2 0.0 4 0 0 0 0 2 137 1 0.0 4 1 143 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 8 0.0 4 0 0 0 0 8 162 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 194 16 0.0 4 0 0 0 1 15 207 3 0.0 4 0 0 0 0 3 208 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 1 237 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.61 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 46,579 Reads with adapters: 1,605 (3.4%) Reads written (passing filters): 46,579 (100.0%) Total basepairs processed: 12,759,688 bp Total written (filtered): 12,671,427 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1605 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 32.5% C: 12.5% G: 52.6% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 250 727.8 0 250 4 21 181.9 0 21 9 1 0.2 0 0 1 10 10 0.0 1 0 10 11 325 0.0 1 7 318 12 509 0.0 1 37 472 13 21 0.0 1 0 21 15 96 0.0 1 54 42 16 3 0.0 1 1 2 20 1 0.0 1 0 1 21 1 0.0 1 0 1 33 2 0.0 1 2 74 2 0.0 1 1 1 112 1 0.0 1 1 113 1 0.0 1 1 125 1 0.0 1 1 128 1 0.0 1 1 152 5 0.0 1 2 3 153 1 0.0 1 0 1 208 1 0.0 1 1 210 322 0.0 1 281 41 211 25 0.0 1 22 3 225 5 0.0 1 4 1