This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.12 s (28 us/read; 2.18 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 246,438 (8.7%) Reads that were too short: 36,536 (1.3%) Reads written (passing filters): 209,902 (7.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 56,134,732 bp (6.6%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 246438 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2490 692.0 0 2490 7 67239 692.0 0 67239 8 2352 692.0 0 2352 9 42371 692.0 0 42371 10 1563 692.0 0 1563 11 41963 692.0 0 41963 12 107 692.0 0 107 13 40 692.0 0 40 14 70 692.0 0 70 15 58 692.0 0 58 16 45 692.0 0 45 17 36 692.0 0 36 18 58 692.0 0 58 19 44 692.0 0 44 20 58 692.0 0 58 21 38 692.0 0 38 22 35 692.0 0 35 23 49 692.0 0 49 24 62 692.0 0 62 25 56 692.0 0 56 26 67 692.0 0 67 27 116 692.0 0 116 28 108 692.0 0 108 29 119 692.0 0 119 30 614 692.0 0 614 31 54 692.0 0 54 32 67 692.0 0 67 33 47 692.0 0 47 34 47 692.0 0 47 35 43 692.0 0 43 36 72 692.0 0 72 37 55 692.0 0 55 38 61 692.0 0 61 39 62 692.0 0 62 40 54 692.0 0 54 41 148 692.0 0 148 42 69 692.0 0 69 43 57 692.0 0 57 44 78 692.0 0 78 45 63 692.0 0 63 46 77 692.0 0 77 47 62 692.0 0 62 48 81 692.0 0 81 49 79 692.0 0 79 50 269 692.0 0 269 51 40 692.0 0 40 52 65 692.0 0 65 53 49 692.0 0 49 54 56 692.0 0 56 55 89 692.0 0 89 56 92 692.0 0 92 57 123 692.0 0 123 58 46 692.0 0 46 59 63 692.0 0 63 60 79 692.0 0 79 61 64 692.0 0 64 62 79 692.0 0 79 63 65 692.0 0 65 64 81 692.0 0 81 65 74 692.0 0 74 66 76 692.0 0 76 67 113 692.0 0 113 68 72 692.0 0 72 69 98 692.0 0 98 70 281 692.0 0 281 71 144 692.0 0 144 72 800 692.0 0 800 73 76 692.0 0 76 74 80 692.0 0 80 75 97 692.0 0 97 76 91 692.0 0 91 77 104 692.0 0 104 78 123 692.0 0 123 79 495 692.0 0 495 80 1698 692.0 0 1698 81 198 692.0 0 198 82 135 692.0 0 135 83 1349 692.0 0 1349 84 1237 692.0 0 1237 85 3753 692.0 0 3753 86 81 692.0 0 81 87 44 692.0 0 44 88 61 692.0 0 61 89 66 692.0 0 66 90 97 692.0 0 97 91 70 692.0 0 70 92 51 692.0 0 51 93 41 692.0 0 41 94 41 692.0 0 41 95 69 692.0 0 69 96 64 692.0 0 64 97 113 692.0 0 113 98 123 692.0 0 123 99 298 692.0 0 298 100 300 692.0 0 300 101 109 692.0 0 109 102 142 692.0 0 142 103 158 692.0 0 158 104 110 692.0 0 110 105 139 692.0 0 139 106 464 692.0 0 464 107 182 692.0 0 182 108 189 692.0 0 189 109 157 692.0 0 157 110 220 692.0 0 220 111 281 692.0 0 281 112 365 692.0 0 365 113 4503 692.0 0 4503 114 3043 692.0 0 3043 115 13531 692.0 0 13531 116 132 692.0 0 132 117 53 692.0 0 53 118 46 692.0 0 46 119 56 692.0 0 56 120 45 692.0 0 45 121 54 692.0 0 54 122 57 692.0 0 57 123 66 692.0 0 66 124 82 692.0 0 82 125 72 692.0 0 72 126 185 692.0 0 185 127 83 692.0 0 83 128 91 692.0 0 91 129 72 692.0 0 72 130 102 692.0 0 102 131 145 692.0 0 145 132 56 692.0 0 56 133 110 692.0 0 110 134 91 692.0 0 91 135 174 692.0 0 174 136 205 692.0 0 205 137 82 692.0 0 82 138 135 692.0 0 135 139 168 692.0 0 168 140 141 692.0 0 141 141 140 692.0 0 140 142 170 692.0 0 170 143 100 692.0 0 100 144 75 692.0 0 75 145 87 692.0 0 87 146 188 692.0 0 188 147 78 692.0 0 78 148 105 692.0 0 105 149 97 692.0 0 97 150 350 692.0 0 350 151 65 692.0 0 65 152 68 692.0 0 68 153 91 692.0 0 91 154 1035 692.0 0 1035 155 655 692.0 0 655 156 532 692.0 0 532 157 807 692.0 0 807 158 156 692.0 0 156 159 74 692.0 0 74 160 77 692.0 0 77 161 66 692.0 0 66 162 58 692.0 0 58 163 180 692.0 0 180 164 65 692.0 0 65 165 122 692.0 0 122 166 56 692.0 0 56 167 387 692.0 0 387 168 113 692.0 0 113 169 79 692.0 0 79 170 184 692.0 0 184 171 67 692.0 0 67 172 58 692.0 0 58 173 87 692.0 0 87 174 48 692.0 0 48 175 48 692.0 0 48 176 54 692.0 0 54 177 53 692.0 0 53 178 55 692.0 0 55 179 287 692.0 0 287 180 41 692.0 0 41 181 44 692.0 0 44 182 80 692.0 0 80 183 51 692.0 0 51 184 120 692.0 0 120 185 106 692.0 0 106 186 102 692.0 0 102 187 95 692.0 0 95 188 66 692.0 0 66 189 134 692.0 0 134 190 57 692.0 0 57 191 61 692.0 0 61 192 70 692.0 0 70 193 68 692.0 0 68 194 70 692.0 0 70 195 66 692.0 0 66 196 69 692.0 0 69 197 88 692.0 0 88 198 61 692.0 0 61 199 65 692.0 0 65 200 253 692.0 0 253 201 115 692.0 0 115 202 152 692.0 0 152 203 56 692.0 0 56 204 76 692.0 0 76 205 49 692.0 0 49 206 58 692.0 0 58 207 68 692.0 0 68 208 59 692.0 0 59 209 71 692.0 0 71 210 84 692.0 0 84 211 375 692.0 0 375 212 353 692.0 0 353 213 1127 692.0 0 1127 214 133 692.0 0 133 215 103 692.0 0 103 216 90 692.0 0 90 217 78 692.0 0 78 218 74 692.0 0 74 219 51 692.0 0 51 220 60 692.0 0 60 221 84 692.0 0 84 222 75 692.0 0 75 223 83 692.0 0 83 224 60 692.0 0 60 225 77 692.0 0 77 226 91 692.0 0 91 227 135 692.0 0 135 228 1229 692.0 0 1229 229 406 692.0 0 406 230 1204 692.0 0 1204 231 119 692.0 0 119 232 226 692.0 0 226 233 97 692.0 0 97 234 132 692.0 0 132 235 187 692.0 0 187 236 276 692.0 0 276 237 172 692.0 0 172 238 143 692.0 0 143 239 64 692.0 0 64 240 72 692.0 0 72 241 75 692.0 0 75 242 52 692.0 0 52 243 87 692.0 0 87 244 95 692.0 0 95 245 74 692.0 0 74 246 138 692.0 0 138 247 196 692.0 0 196 248 362 692.0 0 362 249 173 692.0 0 173 250 334 692.0 0 334 251 87 692.0 0 87 252 104 692.0 0 104 253 95 692.0 0 95 254 190 692.0 0 190 255 165 692.0 0 165 256 221 692.0 0 221 257 330 692.0 0 330 258 169 692.0 0 169 259 69 692.0 0 69 260 81 692.0 0 81 261 64 692.0 0 64 262 83 692.0 0 83 263 62 692.0 0 62 264 100 692.0 0 100 265 97 692.0 0 97 266 59 692.0 0 59 267 89 692.0 0 89 268 195 692.0 0 195 269 697 692.0 0 697 270 607 692.0 0 607 271 271 692.0 0 271 272 129 692.0 0 129 273 83 692.0 0 83 274 240 692.0 0 240 275 80 692.0 0 80 276 79 692.0 0 79 277 93 692.0 0 93 278 118 692.0 0 118 279 111 692.0 0 111 280 481 692.0 0 481 281 200 692.0 0 200 282 232 692.0 0 232 283 180 692.0 0 180 284 153 692.0 0 153 285 238 692.0 0 238 286 105 692.0 0 105 287 109 692.0 0 109 288 192 692.0 0 192 289 174 692.0 0 174 290 187 692.0 0 187 291 138 692.0 0 138 292 183 692.0 0 183 293 251 692.0 0 251 294 486 692.0 0 486 295 1257 692.0 0 1257 296 5834 692.0 0 5834 297 2537 692.0 0 2537 298 5675 692.0 0 5675 299 1261 692.0 0 1261 300 2092 692.0 0 2092 301 168 692.0 0 168 Finished in 10.73 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 209,902 Reads with adapters: 45,391 (21.6%) Reads that were too short: 62 (0.0%) Reads written (passing filters): 45,329 (21.6%) Total basepairs processed: 56,134,732 bp Total written (filtered): 12,332,427 bp (22.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45391 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 12 0.0 3 0 0 0 5 7 18 58 0.0 3 0 0 22 20 16 19 616 0.0 3 0 488 46 64 18 20 44102 0.0 4 40750 2611 540 165 36 21 104 0.0 4 29 59 6 1 9 22 13 0.0 4 1 0 5 2 5 23 395 0.0 4 0 0 1 288 106 24 2 0.0 4 1 0 0 0 1 26 1 0.0 4 1 27 2 0.0 4 0 0 0 0 2 29 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 49 1 0.0 4 0 1 57 1 0.0 4 0 0 1 63 1 0.0 4 1 76 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 1 85 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 1 90 2 0.0 4 0 1 0 0 1 91 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 1 95 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 1 118 1 0.0 4 0 0 0 1 120 2 0.0 4 0 0 0 0 2 121 18 0.0 4 0 1 0 0 17 122 1 0.0 4 1 127 2 0.0 4 0 0 0 0 2 136 2 0.0 4 1 1 138 1 0.0 4 1 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 151 6 0.0 4 0 0 0 0 6 152 2 0.0 4 0 0 0 0 2 153 6 0.0 4 0 0 0 0 6 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 183 2 0.0 4 1 0 0 0 1 198 2 0.0 4 0 1 0 1 203 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 1 1 214 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.64 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 45,329 Reads with adapters: 10,595 (23.4%) Reads written (passing filters): 45,329 (100.0%) Total basepairs processed: 12,332,427 bp Total written (filtered): 12,188,717 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10595 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 8.6% G: 84.7% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 708.3 0 11 4 6 177.1 0 6 6 3 11.1 0 3 8 1 0.7 0 1 9 538 0.2 0 47 491 10 9089 0.0 1 740 8349 11 707 0.0 1 37 670 12 12 0.0 1 0 12 13 33 0.0 1 0 33 16 2 0.0 1 0 2 17 1 0.0 1 1 22 1 0.0 1 1 63 1 0.0 1 1 139 1 0.0 1 1 150 2 0.0 1 1 1 153 2 0.0 1 2 173 1 0.0 1 0 1 191 2 0.0 1 0 2 195 1 0.0 1 0 1 199 1 0.0 1 0 1 207 1 0.0 1 1 208 122 0.0 1 99 23 209 11 0.0 1 10 1 210 2 0.0 1 2 211 41 0.0 1 35 6 223 1 0.0 1 1 270 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.