This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.78 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 249,618 (8.8%) Reads that were too short: 27,635 (1.0%) Reads written (passing filters): 221,983 (7.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 59,398,197 bp (7.0%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 249618 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2516 692.0 0 2516 7 53403 692.0 0 53403 8 2096 692.0 0 2096 9 59019 692.0 0 59019 10 1245 692.0 0 1245 11 50214 692.0 0 50214 12 99 692.0 0 99 13 36 692.0 0 36 14 37 692.0 0 37 15 38 692.0 0 38 16 36 692.0 0 36 17 42 692.0 0 42 18 40 692.0 0 40 19 40 692.0 0 40 20 36 692.0 0 36 21 48 692.0 0 48 22 53 692.0 0 53 23 53 692.0 0 53 24 51 692.0 0 51 25 47 692.0 0 47 26 50 692.0 0 50 27 40 692.0 0 40 28 68 692.0 0 68 29 77 692.0 0 77 30 634 692.0 0 634 31 45 692.0 0 45 32 49 692.0 0 49 33 49 692.0 0 49 34 52 692.0 0 52 35 55 692.0 0 55 36 54 692.0 0 54 37 51 692.0 0 51 38 69 692.0 0 69 39 79 692.0 0 79 40 57 692.0 0 57 41 183 692.0 0 183 42 90 692.0 0 90 43 39 692.0 0 39 44 63 692.0 0 63 45 58 692.0 0 58 46 68 692.0 0 68 47 59 692.0 0 59 48 72 692.0 0 72 49 74 692.0 0 74 50 283 692.0 0 283 51 54 692.0 0 54 52 57 692.0 0 57 53 70 692.0 0 70 54 63 692.0 0 63 55 95 692.0 0 95 56 104 692.0 0 104 57 62 692.0 0 62 58 75 692.0 0 75 59 62 692.0 0 62 60 80 692.0 0 80 61 69 692.0 0 69 62 93 692.0 0 93 63 79 692.0 0 79 64 69 692.0 0 69 65 77 692.0 0 77 66 81 692.0 0 81 67 122 692.0 0 122 68 81 692.0 0 81 69 89 692.0 0 89 70 284 692.0 0 284 71 138 692.0 0 138 72 752 692.0 0 752 73 83 692.0 0 83 74 70 692.0 0 70 75 81 692.0 0 81 76 64 692.0 0 64 77 87 692.0 0 87 78 107 692.0 0 107 79 363 692.0 0 363 80 1823 692.0 0 1823 81 168 692.0 0 168 82 137 692.0 0 137 83 1438 692.0 0 1438 84 1358 692.0 0 1358 85 4088 692.0 0 4088 86 81 692.0 0 81 87 58 692.0 0 58 88 50 692.0 0 50 89 70 692.0 0 70 90 72 692.0 0 72 91 69 692.0 0 69 92 58 692.0 0 58 93 55 692.0 0 55 94 44 692.0 0 44 95 64 692.0 0 64 96 82 692.0 0 82 97 106 692.0 0 106 98 121 692.0 0 121 99 315 692.0 0 315 100 329 692.0 0 329 101 137 692.0 0 137 102 167 692.0 0 167 103 180 692.0 0 180 104 85 692.0 0 85 105 156 692.0 0 156 106 509 692.0 0 509 107 158 692.0 0 158 108 204 692.0 0 204 109 127 692.0 0 127 110 216 692.0 0 216 111 259 692.0 0 259 112 329 692.0 0 329 113 4476 692.0 0 4476 114 2731 692.0 0 2731 115 14494 692.0 0 14494 116 114 692.0 0 114 117 52 692.0 0 52 118 58 692.0 0 58 119 50 692.0 0 50 120 72 692.0 0 72 121 50 692.0 0 50 122 40 692.0 0 40 123 70 692.0 0 70 124 65 692.0 0 65 125 63 692.0 0 63 126 153 692.0 0 153 127 65 692.0 0 65 128 88 692.0 0 88 129 59 692.0 0 59 130 93 692.0 0 93 131 140 692.0 0 140 132 50 692.0 0 50 133 81 692.0 0 81 134 61 692.0 0 61 135 136 692.0 0 136 136 117 692.0 0 117 137 57 692.0 0 57 138 61 692.0 0 61 139 100 692.0 0 100 140 112 692.0 0 112 141 119 692.0 0 119 142 173 692.0 0 173 143 75 692.0 0 75 144 75 692.0 0 75 145 81 692.0 0 81 146 228 692.0 0 228 147 81 692.0 0 81 148 102 692.0 0 102 149 67 692.0 0 67 150 386 692.0 0 386 151 78 692.0 0 78 152 73 692.0 0 73 153 86 692.0 0 86 154 1202 692.0 0 1202 155 787 692.0 0 787 156 700 692.0 0 700 157 1069 692.0 0 1069 158 186 692.0 0 186 159 88 692.0 0 88 160 88 692.0 0 88 161 53 692.0 0 53 162 43 692.0 0 43 163 202 692.0 0 202 164 57 692.0 0 57 165 119 692.0 0 119 166 88 692.0 0 88 167 464 692.0 0 464 168 77 692.0 0 77 169 72 692.0 0 72 170 277 692.0 0 277 171 65 692.0 0 65 172 68 692.0 0 68 173 98 692.0 0 98 174 64 692.0 0 64 175 70 692.0 0 70 176 58 692.0 0 58 177 67 692.0 0 67 178 79 692.0 0 79 179 421 692.0 0 421 180 56 692.0 0 56 181 67 692.0 0 67 182 92 692.0 0 92 183 67 692.0 0 67 184 129 692.0 0 129 185 106 692.0 0 106 186 94 692.0 0 94 187 91 692.0 0 91 188 43 692.0 0 43 189 169 692.0 0 169 190 64 692.0 0 64 191 66 692.0 0 66 192 55 692.0 0 55 193 52 692.0 0 52 194 55 692.0 0 55 195 60 692.0 0 60 196 69 692.0 0 69 197 79 692.0 0 79 198 64 692.0 0 64 199 68 692.0 0 68 200 276 692.0 0 276 201 76 692.0 0 76 202 137 692.0 0 137 203 60 692.0 0 60 204 53 692.0 0 53 205 39 692.0 0 39 206 60 692.0 0 60 207 47 692.0 0 47 208 66 692.0 0 66 209 51 692.0 0 51 210 58 692.0 0 58 211 111 692.0 0 111 212 98 692.0 0 98 213 269 692.0 0 269 214 104 692.0 0 104 215 69 692.0 0 69 216 92 692.0 0 92 217 56 692.0 0 56 218 62 692.0 0 62 219 45 692.0 0 45 220 62 692.0 0 62 221 72 692.0 0 72 222 67 692.0 0 67 223 72 692.0 0 72 224 54 692.0 0 54 225 73 692.0 0 73 226 62 692.0 0 62 227 65 692.0 0 65 228 354 692.0 0 354 229 151 692.0 0 151 230 303 692.0 0 303 231 88 692.0 0 88 232 143 692.0 0 143 233 63 692.0 0 63 234 76 692.0 0 76 235 75 692.0 0 75 236 151 692.0 0 151 237 92 692.0 0 92 238 63 692.0 0 63 239 78 692.0 0 78 240 56 692.0 0 56 241 59 692.0 0 59 242 47 692.0 0 47 243 68 692.0 0 68 244 60 692.0 0 60 245 59 692.0 0 59 246 74 692.0 0 74 247 82 692.0 0 82 248 208 692.0 0 208 249 113 692.0 0 113 250 421 692.0 0 421 251 66 692.0 0 66 252 77 692.0 0 77 253 75 692.0 0 75 254 75 692.0 0 75 255 69 692.0 0 69 256 92 692.0 0 92 257 109 692.0 0 109 258 94 692.0 0 94 259 55 692.0 0 55 260 81 692.0 0 81 261 41 692.0 0 41 262 64 692.0 0 64 263 56 692.0 0 56 264 71 692.0 0 71 265 60 692.0 0 60 266 54 692.0 0 54 267 118 692.0 0 118 268 108 692.0 0 108 269 261 692.0 0 261 270 209 692.0 0 209 271 124 692.0 0 124 272 78 692.0 0 78 273 60 692.0 0 60 274 94 692.0 0 94 275 32 692.0 0 32 276 77 692.0 0 77 277 59 692.0 0 59 278 90 692.0 0 90 279 76 692.0 0 76 280 143 692.0 0 143 281 151 692.0 0 151 282 120 692.0 0 120 283 139 692.0 0 139 284 151 692.0 0 151 285 229 692.0 0 229 286 88 692.0 0 88 287 102 692.0 0 102 288 150 692.0 0 150 289 142 692.0 0 142 290 108 692.0 0 108 291 140 692.0 0 140 292 129 692.0 0 129 293 160 692.0 0 160 294 302 692.0 0 302 295 839 692.0 0 839 296 3666 692.0 0 3666 297 1519 692.0 0 1519 298 6737 692.0 0 6737 299 1794 692.0 0 1794 300 3314 692.0 0 3314 301 99 692.0 0 99 Finished in 10.30 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 221,983 Reads with adapters: 52,821 (23.8%) Reads that were too short: 110 (0.0%) Reads written (passing filters): 52,711 (23.7%) Total basepairs processed: 59,398,197 bp Total written (filtered): 14,581,551 bp (24.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 52821 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 287 0.0 3 0 280 7 17 51978 0.0 3 50943 1004 28 3 18 117 0.0 3 10 100 6 1 19 22 0.0 3 6 2 8 6 20 157 0.0 3 0 0 0 157 21 3 0.0 3 1 0 0 2 22 2 0.0 3 1 1 23 10 0.0 3 1 0 0 9 25 1 0.0 3 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 30 0.0 3 0 0 0 30 35 4 0.0 3 0 0 0 4 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 9 0.0 3 0 0 0 9 51 4 0.0 3 1 0 0 3 52 1 0.0 3 0 0 0 1 53 6 0.0 3 0 0 0 6 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 73 40 0.0 3 0 0 38 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 1 0.0 3 1 82 4 0.0 3 1 1 0 2 86 1 0.0 3 0 1 88 1 0.0 3 0 0 0 1 89 3 0.0 3 3 90 1 0.0 3 1 98 1 0.0 3 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 1 0.0 3 1 122 1 0.0 3 0 0 0 1 125 5 0.0 3 0 0 0 5 129 1 0.0 3 1 134 1 0.0 3 1 138 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 168 2 0.0 3 0 1 0 1 170 1 0.0 3 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 1 174 2 0.0 3 2 175 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 2 0.0 3 0 1 0 1 188 1 0.0 3 0 0 0 1 190 4 0.0 3 0 0 1 3 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 197 1 0.0 3 1 200 25 0.0 3 0 0 0 25 209 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 224 1 0.0 3 1 235 2 0.0 3 0 0 0 2 236 14 0.0 3 0 0 0 14 244 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 3 0.0 3 0 0 0 3 278 5 0.0 3 0 0 0 5 279 4 0.0 3 0 0 0 4 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.91 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 52,711 Reads with adapters: 151 (0.3%) Reads written (passing filters): 52,711 (100.0%) Total basepairs processed: 14,581,551 bp Total written (filtered): 14,581,064 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 151 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.8% C: 24.5% G: 30.5% T: 9.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 129 823.6 0 129 4 16 205.9 0 16 5 2 51.5 0 2 6 3 12.9 0 3 8 1 0.8 0 1