This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.89 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 212,960 (7.5%) Reads that were too short: 9,556 (0.3%) Reads written (passing filters): 203,404 (7.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 49,084,322 bp (5.8%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 212960 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1176 692.0 0 1176 7 5196 692.0 0 5196 8 48940 692.0 0 48940 9 41707 692.0 0 41707 10 70 692.0 0 70 11 39 692.0 0 39 12 57 692.0 0 57 13 29 692.0 0 29 14 30 692.0 0 30 15 23 692.0 0 23 16 38 692.0 0 38 17 30 692.0 0 30 18 23 692.0 0 23 19 26 692.0 0 26 20 40 692.0 0 40 21 59 692.0 0 59 22 37 692.0 0 37 23 30 692.0 0 30 24 74 692.0 0 74 25 76 692.0 0 76 26 63 692.0 0 63 27 30 692.0 0 30 28 40 692.0 0 40 29 49 692.0 0 49 30 104 692.0 0 104 31 55 692.0 0 55 32 91 692.0 0 91 33 36 692.0 0 36 34 109 692.0 0 109 35 91 692.0 0 91 36 252 692.0 0 252 37 77 692.0 0 77 38 461 692.0 0 461 39 45 692.0 0 45 40 48 692.0 0 48 41 39 692.0 0 39 42 42 692.0 0 42 43 24 692.0 0 24 44 39 692.0 0 39 45 51 692.0 0 51 46 53 692.0 0 53 47 78 692.0 0 78 48 241 692.0 0 241 49 37 692.0 0 37 50 40 692.0 0 40 51 55 692.0 0 55 52 34 692.0 0 34 53 74 692.0 0 74 54 119 692.0 0 119 55 163 692.0 0 163 56 29 692.0 0 29 57 46 692.0 0 46 58 28 692.0 0 28 59 25 692.0 0 25 60 41 692.0 0 41 61 70 692.0 0 70 62 43 692.0 0 43 63 82 692.0 0 82 64 140 692.0 0 140 65 70 692.0 0 70 66 133 692.0 0 133 67 58 692.0 0 58 68 49 692.0 0 49 69 70 692.0 0 70 70 59 692.0 0 59 71 59 692.0 0 59 72 58 692.0 0 58 73 59 692.0 0 59 74 70 692.0 0 70 75 120 692.0 0 120 76 170 692.0 0 170 77 271 692.0 0 271 78 354 692.0 0 354 79 780 692.0 0 780 80 1742 692.0 0 1742 81 32653 692.0 0 32653 82 2613 692.0 0 2613 83 6744 692.0 0 6744 84 93 692.0 0 93 85 310 692.0 0 310 86 236 692.0 0 236 87 863 692.0 0 863 88 80 692.0 0 80 89 42 692.0 0 42 90 31 692.0 0 31 91 26 692.0 0 26 92 58 692.0 0 58 93 23 692.0 0 23 94 33 692.0 0 33 95 86 692.0 0 86 96 59 692.0 0 59 97 50 692.0 0 50 98 86 692.0 0 86 99 45 692.0 0 45 100 41 692.0 0 41 101 53 692.0 0 53 102 165 692.0 0 165 103 77 692.0 0 77 104 157 692.0 0 157 105 60 692.0 0 60 106 84 692.0 0 84 107 114 692.0 0 114 108 240 692.0 0 240 109 463 692.0 0 463 110 153 692.0 0 153 111 150 692.0 0 150 112 585 692.0 0 585 113 129 692.0 0 129 114 91 692.0 0 91 115 178 692.0 0 178 116 226 692.0 0 226 117 226 692.0 0 226 118 198 692.0 0 198 119 531 692.0 0 531 120 494 692.0 0 494 121 1082 692.0 0 1082 122 9538 692.0 0 9538 123 3398 692.0 0 3398 124 22635 692.0 0 22635 125 129 692.0 0 129 126 120 692.0 0 120 127 50 692.0 0 50 128 35 692.0 0 35 129 72 692.0 0 72 130 72 692.0 0 72 131 74 692.0 0 74 132 29 692.0 0 29 133 71 692.0 0 71 134 52 692.0 0 52 135 59 692.0 0 59 136 54 692.0 0 54 137 57 692.0 0 57 138 58 692.0 0 58 139 56 692.0 0 56 140 72 692.0 0 72 141 59 692.0 0 59 142 199 692.0 0 199 143 74 692.0 0 74 144 248 692.0 0 248 145 185 692.0 0 185 146 845 692.0 0 845 147 191 692.0 0 191 148 120 692.0 0 120 149 151 692.0 0 151 150 171 692.0 0 171 151 233 692.0 0 233 152 109 692.0 0 109 153 80 692.0 0 80 154 54 692.0 0 54 155 95 692.0 0 95 156 44 692.0 0 44 157 46 692.0 0 46 158 86 692.0 0 86 159 52 692.0 0 52 160 67 692.0 0 67 161 33 692.0 0 33 162 34 692.0 0 34 163 61 692.0 0 61 164 59 692.0 0 59 165 118 692.0 0 118 166 218 692.0 0 218 167 95 692.0 0 95 168 948 692.0 0 948 169 39 692.0 0 39 170 35 692.0 0 35 171 54 692.0 0 54 172 69 692.0 0 69 173 57 692.0 0 57 174 32 692.0 0 32 175 46 692.0 0 46 176 50 692.0 0 50 177 55 692.0 0 55 178 66 692.0 0 66 179 57 692.0 0 57 180 125 692.0 0 125 181 244 692.0 0 244 182 4193 692.0 0 4193 183 313 692.0 0 313 184 953 692.0 0 953 185 119 692.0 0 119 186 36 692.0 0 36 187 51 692.0 0 51 188 42 692.0 0 42 189 53 692.0 0 53 190 40 692.0 0 40 191 33 692.0 0 33 192 42 692.0 0 42 193 80 692.0 0 80 194 92 692.0 0 92 195 135 692.0 0 135 196 47 692.0 0 47 197 33 692.0 0 33 198 97 692.0 0 97 199 83 692.0 0 83 200 106 692.0 0 106 201 27 692.0 0 27 202 24 692.0 0 24 203 24 692.0 0 24 204 32 692.0 0 32 205 85 692.0 0 85 206 45 692.0 0 45 207 51 692.0 0 51 208 24 692.0 0 24 209 68 692.0 0 68 210 61 692.0 0 61 211 96 692.0 0 96 212 55 692.0 0 55 213 31 692.0 0 31 214 38 692.0 0 38 215 37 692.0 0 37 216 29 692.0 0 29 217 34 692.0 0 34 218 30 692.0 0 30 219 68 692.0 0 68 220 56 692.0 0 56 221 63 692.0 0 63 222 38 692.0 0 38 223 24 692.0 0 24 224 40 692.0 0 40 225 36 692.0 0 36 226 89 692.0 0 89 227 41 692.0 0 41 228 93 692.0 0 93 229 38 692.0 0 38 230 41 692.0 0 41 231 46 692.0 0 46 232 34 692.0 0 34 233 40 692.0 0 40 234 42 692.0 0 42 235 35 692.0 0 35 236 43 692.0 0 43 237 38 692.0 0 38 238 48 692.0 0 48 239 45 692.0 0 45 240 144 692.0 0 144 241 117 692.0 0 117 242 102 692.0 0 102 243 44 692.0 0 44 244 59 692.0 0 59 245 33 692.0 0 33 246 54 692.0 0 54 247 56 692.0 0 56 248 51 692.0 0 51 249 62 692.0 0 62 250 57 692.0 0 57 251 40 692.0 0 40 252 42 692.0 0 42 253 55 692.0 0 55 254 64 692.0 0 64 255 95 692.0 0 95 256 70 692.0 0 70 257 39 692.0 0 39 258 86 692.0 0 86 259 95 692.0 0 95 260 82 692.0 0 82 261 134 692.0 0 134 262 166 692.0 0 166 263 185 692.0 0 185 264 63 692.0 0 63 265 64 692.0 0 64 266 452 692.0 0 452 267 309 692.0 0 309 268 1109 692.0 0 1109 269 142 692.0 0 142 270 75 692.0 0 75 271 40 692.0 0 40 272 72 692.0 0 72 273 44 692.0 0 44 274 213 692.0 0 213 275 175 692.0 0 175 276 173 692.0 0 173 277 196 692.0 0 196 278 79 692.0 0 79 279 180 692.0 0 180 280 110 692.0 0 110 281 181 692.0 0 181 282 103 692.0 0 103 283 55 692.0 0 55 284 30 692.0 0 30 285 47 692.0 0 47 286 77 692.0 0 77 287 150 692.0 0 150 288 348 692.0 0 348 289 138 692.0 0 138 290 124 692.0 0 124 291 71 692.0 0 71 292 90 692.0 0 90 293 96 692.0 0 96 294 223 692.0 0 223 295 97 692.0 0 97 296 143 692.0 0 143 297 56 692.0 0 56 298 78 692.0 0 78 299 93 692.0 0 93 300 95 692.0 0 95 301 71 692.0 0 71 Finished in 9.75 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 203,404 Reads with adapters: 47,446 (23.3%) Reads that were too short: 77 (0.0%) Reads written (passing filters): 47,369 (23.3%) Total basepairs processed: 49,084,322 bp Total written (filtered): 12,813,075 bp (26.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 47446 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 4 3 18 39 0.0 3 0 0 21 9 9 19 809 0.0 3 0 720 41 40 8 20 42201 0.0 4 39523 2114 403 130 31 21 138 0.0 4 24 92 14 5 3 22 2845 0.0 4 2585 183 45 16 16 23 603 0.0 4 231 14 4 266 88 24 5 0.0 4 2 0 0 0 3 25 26 0.0 4 0 0 0 17 9 26 4 0.0 4 1 0 0 0 3 28 1 0.0 4 0 1 32 1 0.0 4 0 0 0 0 1 48 2 0.0 4 0 0 0 0 2 54 1 0.0 4 0 1 75 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 1 92 1 0.0 4 0 0 0 0 1 93 3 0.0 4 1 0 1 0 1 99 1 0.0 4 0 0 0 0 1 100 4 0.0 4 0 0 0 0 4 101 3 0.0 4 0 0 0 0 3 122 1 0.0 4 0 1 125 3 0.0 4 0 0 0 0 3 126 4 0.0 4 0 0 0 0 4 127 667 0.0 4 0 0 0 1 666 128 2 0.0 4 0 0 0 0 2 130 3 0.0 4 0 0 0 0 3 142 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 4 0.0 4 0 0 0 1 3 149 7 0.0 4 0 0 0 0 7 150 8 0.0 4 0 0 0 0 8 151 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 1 0 0 1 178 1 0.0 4 0 0 0 0 1 179 4 0.0 4 0 0 0 0 4 182 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 2 0.0 4 1 0 0 0 1 191 2 0.0 4 0 1 0 0 1 192 1 0.0 4 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 1 2 236 2 0.0 4 0 0 0 0 2 249 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.72 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 47,369 Reads with adapters: 10,764 (22.7%) Reads written (passing filters): 47,369 (100.0%) Total basepairs processed: 12,813,075 bp Total written (filtered): 12,691,046 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10764 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 7.6% G: 87.8% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 740.1 0 17 4 6 185.0 0 6 6 1 11.6 0 1 7 2 2.9 0 2 9 1518 0.2 0 168 1350 10 8044 0.0 1 631 7413 11 1027 0.0 1 43 984 12 30 0.0 1 0 30 13 35 0.0 1 0 35 14 2 0.0 1 0 2 92 1 0.0 1 0 1 150 9 0.0 1 5 4 158 1 0.0 1 0 1 167 1 0.0 1 1 169 14 0.0 1 14 170 1 0.0 1 1 177 1 0.0 1 1 191 1 0.0 1 0 1 200 9 0.0 1 8 1 201 1 0.0 1 1 208 34 0.0 1 23 11 209 5 0.0 1 5 210 1 0.0 1 1 211 2 0.0 1 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.