This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.89 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 222,591 (7.9%) Reads that were too short: 8,938 (0.3%) Reads written (passing filters): 213,653 (7.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 51,517,043 bp (6.0%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 222591 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1065 692.0 0 1065 7 4937 692.0 0 4937 8 45631 692.0 0 45631 9 50101 692.0 0 50101 10 89 692.0 0 89 11 34 692.0 0 34 12 27 692.0 0 27 13 37 692.0 0 37 14 19 692.0 0 19 15 22 692.0 0 22 16 34 692.0 0 34 17 29 692.0 0 29 18 35 692.0 0 35 19 28 692.0 0 28 20 28 692.0 0 28 21 39 692.0 0 39 22 47 692.0 0 47 23 32 692.0 0 32 24 34 692.0 0 34 25 38 692.0 0 38 26 36 692.0 0 36 27 22 692.0 0 22 28 52 692.0 0 52 29 41 692.0 0 41 30 99 692.0 0 99 31 45 692.0 0 45 32 89 692.0 0 89 33 37 692.0 0 37 34 79 692.0 0 79 35 70 692.0 0 70 36 183 692.0 0 183 37 77 692.0 0 77 38 512 692.0 0 512 39 61 692.0 0 61 40 37 692.0 0 37 41 40 692.0 0 40 42 73 692.0 0 73 43 31 692.0 0 31 44 39 692.0 0 39 45 41 692.0 0 41 46 71 692.0 0 71 47 66 692.0 0 66 48 294 692.0 0 294 49 45 692.0 0 45 50 36 692.0 0 36 51 63 692.0 0 63 52 41 692.0 0 41 53 62 692.0 0 62 54 130 692.0 0 130 55 81 692.0 0 81 56 44 692.0 0 44 57 44 692.0 0 44 58 38 692.0 0 38 59 45 692.0 0 45 60 63 692.0 0 63 61 81 692.0 0 81 62 56 692.0 0 56 63 74 692.0 0 74 64 152 692.0 0 152 65 89 692.0 0 89 66 104 692.0 0 104 67 71 692.0 0 71 68 68 692.0 0 68 69 77 692.0 0 77 70 64 692.0 0 64 71 72 692.0 0 72 72 64 692.0 0 64 73 78 692.0 0 78 74 78 692.0 0 78 75 95 692.0 0 95 76 158 692.0 0 158 77 263 692.0 0 263 78 311 692.0 0 311 79 725 692.0 0 725 80 1736 692.0 0 1736 81 34886 692.0 0 34886 82 2842 692.0 0 2842 83 7696 692.0 0 7696 84 84 692.0 0 84 85 174 692.0 0 174 86 138 692.0 0 138 87 258 692.0 0 258 88 62 692.0 0 62 89 42 692.0 0 42 90 66 692.0 0 66 91 33 692.0 0 33 92 46 692.0 0 46 93 46 692.0 0 46 94 45 692.0 0 45 95 43 692.0 0 43 96 70 692.0 0 70 97 55 692.0 0 55 98 106 692.0 0 106 99 44 692.0 0 44 100 58 692.0 0 58 101 84 692.0 0 84 102 121 692.0 0 121 103 74 692.0 0 74 104 116 692.0 0 116 105 86 692.0 0 86 106 109 692.0 0 109 107 138 692.0 0 138 108 310 692.0 0 310 109 498 692.0 0 498 110 174 692.0 0 174 111 179 692.0 0 179 112 645 692.0 0 645 113 139 692.0 0 139 114 116 692.0 0 116 115 194 692.0 0 194 116 285 692.0 0 285 117 198 692.0 0 198 118 157 692.0 0 157 119 475 692.0 0 475 120 428 692.0 0 428 121 703 692.0 0 703 122 10008 692.0 0 10008 123 3417 692.0 0 3417 124 25413 692.0 0 25413 125 129 692.0 0 129 126 94 692.0 0 94 127 51 692.0 0 51 128 40 692.0 0 40 129 75 692.0 0 75 130 60 692.0 0 60 131 80 692.0 0 80 132 30 692.0 0 30 133 46 692.0 0 46 134 54 692.0 0 54 135 49 692.0 0 49 136 49 692.0 0 49 137 41 692.0 0 41 138 51 692.0 0 51 139 67 692.0 0 67 140 46 692.0 0 46 141 42 692.0 0 42 142 207 692.0 0 207 143 68 692.0 0 68 144 203 692.0 0 203 145 170 692.0 0 170 146 670 692.0 0 670 147 168 692.0 0 168 148 82 692.0 0 82 149 76 692.0 0 76 150 154 692.0 0 154 151 101 692.0 0 101 152 98 692.0 0 98 153 85 692.0 0 85 154 47 692.0 0 47 155 66 692.0 0 66 156 40 692.0 0 40 157 41 692.0 0 41 158 86 692.0 0 86 159 44 692.0 0 44 160 62 692.0 0 62 161 54 692.0 0 54 162 44 692.0 0 44 163 58 692.0 0 58 164 39 692.0 0 39 165 147 692.0 0 147 166 256 692.0 0 256 167 89 692.0 0 89 168 1069 692.0 0 1069 169 56 692.0 0 56 170 44 692.0 0 44 171 43 692.0 0 43 172 74 692.0 0 74 173 44 692.0 0 44 174 30 692.0 0 30 175 55 692.0 0 55 176 56 692.0 0 56 177 55 692.0 0 55 178 66 692.0 0 66 179 70 692.0 0 70 180 159 692.0 0 159 181 244 692.0 0 244 182 4660 692.0 0 4660 183 360 692.0 0 360 184 1129 692.0 0 1129 185 106 692.0 0 106 186 62 692.0 0 62 187 66 692.0 0 66 188 39 692.0 0 39 189 46 692.0 0 46 190 49 692.0 0 49 191 35 692.0 0 35 192 32 692.0 0 32 193 55 692.0 0 55 194 41 692.0 0 41 195 70 692.0 0 70 196 39 692.0 0 39 197 39 692.0 0 39 198 66 692.0 0 66 199 51 692.0 0 51 200 71 692.0 0 71 201 43 692.0 0 43 202 38 692.0 0 38 203 31 692.0 0 31 204 37 692.0 0 37 205 60 692.0 0 60 206 55 692.0 0 55 207 34 692.0 0 34 208 39 692.0 0 39 209 50 692.0 0 50 210 36 692.0 0 36 211 43 692.0 0 43 212 59 692.0 0 59 213 38 692.0 0 38 214 51 692.0 0 51 215 43 692.0 0 43 216 33 692.0 0 33 217 50 692.0 0 50 218 38 692.0 0 38 219 67 692.0 0 67 220 73 692.0 0 73 221 58 692.0 0 58 222 40 692.0 0 40 223 39 692.0 0 39 224 46 692.0 0 46 225 41 692.0 0 41 226 41 692.0 0 41 227 47 692.0 0 47 228 90 692.0 0 90 229 51 692.0 0 51 230 58 692.0 0 58 231 41 692.0 0 41 232 43 692.0 0 43 233 47 692.0 0 47 234 42 692.0 0 42 235 46 692.0 0 46 236 41 692.0 0 41 237 58 692.0 0 58 238 56 692.0 0 56 239 48 692.0 0 48 240 146 692.0 0 146 241 134 692.0 0 134 242 68 692.0 0 68 243 35 692.0 0 35 244 38 692.0 0 38 245 30 692.0 0 30 246 42 692.0 0 42 247 59 692.0 0 59 248 58 692.0 0 58 249 66 692.0 0 66 250 66 692.0 0 66 251 41 692.0 0 41 252 64 692.0 0 64 253 75 692.0 0 75 254 57 692.0 0 57 255 83 692.0 0 83 256 72 692.0 0 72 257 46 692.0 0 46 258 90 692.0 0 90 259 71 692.0 0 71 260 93 692.0 0 93 261 131 692.0 0 131 262 163 692.0 0 163 263 127 692.0 0 127 264 73 692.0 0 73 265 87 692.0 0 87 266 514 692.0 0 514 267 326 692.0 0 326 268 1329 692.0 0 1329 269 87 692.0 0 87 270 46 692.0 0 46 271 39 692.0 0 39 272 61 692.0 0 61 273 34 692.0 0 34 274 230 692.0 0 230 275 119 692.0 0 119 276 158 692.0 0 158 277 85 692.0 0 85 278 55 692.0 0 55 279 71 692.0 0 71 280 47 692.0 0 47 281 118 692.0 0 118 282 83 692.0 0 83 283 66 692.0 0 66 284 42 692.0 0 42 285 34 692.0 0 34 286 66 692.0 0 66 287 118 692.0 0 118 288 241 692.0 0 241 289 116 692.0 0 116 290 96 692.0 0 96 291 55 692.0 0 55 292 95 692.0 0 95 293 104 692.0 0 104 294 159 692.0 0 159 295 91 692.0 0 91 296 107 692.0 0 107 297 59 692.0 0 59 298 65 692.0 0 65 299 62 692.0 0 62 300 65 692.0 0 65 301 72 692.0 0 72 Finished in 9.09 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 213,653 Reads with adapters: 47,632 (22.3%) Reads that were too short: 1,123 (0.5%) Reads written (passing filters): 46,509 (21.8%) Total basepairs processed: 51,517,043 bp Total written (filtered): 12,707,257 bp (24.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 47632 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 16 0.0 3 0 0 15 1 16 260 0.0 3 0 255 5 17 45164 0.0 3 44249 880 33 2 18 117 0.0 3 4 110 3 19 36 0.0 3 6 0 29 1 20 79 0.0 3 1 0 0 78 22 3 0.0 3 0 0 0 3 24 1 0.0 3 1 25 45 0.0 3 0 0 41 4 30 1 0.0 3 0 0 0 1 34 1310 0.0 3 0 0 0 1310 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 1 3 48 7 0.0 3 0 0 0 7 49 35 0.0 3 0 0 0 35 50 254 0.0 3 0 0 1 253 51 25 0.0 3 0 0 0 25 52 7 0.0 3 0 1 1 5 53 40 0.0 3 0 0 0 40 54 1 0.0 3 0 0 0 1 64 2 0.0 3 1 0 0 1 69 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 73 5 0.0 3 0 0 0 5 75 2 0.0 3 1 0 0 1 76 4 0.0 3 1 1 1 1 79 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 3 0.0 3 1 0 0 2 91 1 0.0 3 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 1 106 4 0.0 3 0 0 0 4 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 116 1 0.0 3 0 1 118 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 146 2 0.0 3 1 0 0 1 151 1 0.0 3 0 0 0 1 161 5 0.0 3 0 0 0 5 162 29 0.0 3 0 0 3 26 163 13 0.0 3 0 0 0 13 164 12 0.0 3 0 0 0 12 165 79 0.0 3 0 0 4 75 166 9 0.0 3 0 0 0 9 170 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 1 3 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.66 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 46,509 Reads with adapters: 458 (1.0%) Reads written (passing filters): 46,509 (100.0%) Total basepairs processed: 12,707,257 bp Total written (filtered): 12,705,688 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 458 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.4% C: 8.7% G: 37.8% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 271 726.7 0 271 4 184 181.7 0 184 5 2 45.4 0 2 10 1 0.0 1 0 1