This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.28 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 625,411 (22.1%) Reads that were too short: 315,788 (11.1%) Reads written (passing filters): 309,623 (10.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 82,441,420 bp (9.7%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 625411 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 12304 692.0 0 12304 7 87668 692.0 0 87668 8 6444 692.0 0 6444 9 151410 692.0 0 151410 10 159 692.0 0 159 11 22 692.0 0 22 12 29 692.0 0 29 13 37 692.0 0 37 14 33 692.0 0 33 15 30 692.0 0 30 16 18 692.0 0 18 17 25 692.0 0 25 18 18 692.0 0 18 19 29 692.0 0 29 20 27 692.0 0 27 21 21 692.0 0 21 22 25 692.0 0 25 23 19 692.0 0 19 24 17 692.0 0 17 25 15 692.0 0 15 26 15 692.0 0 15 27 16 692.0 0 16 28 19 692.0 0 19 29 22 692.0 0 22 30 21 692.0 0 21 31 230 692.0 0 230 32 50 692.0 0 50 33 44 692.0 0 44 34 30 692.0 0 30 35 36 692.0 0 36 36 30 692.0 0 30 37 23 692.0 0 23 38 20 692.0 0 20 39 30 692.0 0 30 40 19 692.0 0 19 41 25 692.0 0 25 42 18 692.0 0 18 43 33 692.0 0 33 44 17 692.0 0 17 45 32 692.0 0 32 46 23 692.0 0 23 47 24 692.0 0 24 48 38 692.0 0 38 49 18 692.0 0 18 50 16 692.0 0 16 51 16 692.0 0 16 52 23 692.0 0 23 53 20 692.0 0 20 54 26 692.0 0 26 55 23 692.0 0 23 56 35 692.0 0 35 57 48 692.0 0 48 58 39 692.0 0 39 59 50 692.0 0 50 60 26 692.0 0 26 61 37 692.0 0 37 62 19 692.0 0 19 63 26 692.0 0 26 64 33 692.0 0 33 65 139 692.0 0 139 66 89 692.0 0 89 67 47 692.0 0 47 68 13 692.0 0 13 69 13 692.0 0 13 70 20 692.0 0 20 71 27 692.0 0 27 72 24 692.0 0 24 73 25 692.0 0 25 74 27 692.0 0 27 75 95 692.0 0 95 76 166 692.0 0 166 77 101 692.0 0 101 78 30 692.0 0 30 79 20 692.0 0 20 80 15 692.0 0 15 81 22 692.0 0 22 82 35 692.0 0 35 83 17 692.0 0 17 84 32 692.0 0 32 85 24 692.0 0 24 86 23 692.0 0 23 87 22 692.0 0 22 88 19 692.0 0 19 89 38 692.0 0 38 90 44 692.0 0 44 91 59 692.0 0 59 92 42 692.0 0 42 93 79 692.0 0 79 94 40 692.0 0 40 95 35 692.0 0 35 96 36 692.0 0 36 97 36 692.0 0 36 98 44 692.0 0 44 99 65 692.0 0 65 100 79 692.0 0 79 101 33 692.0 0 33 102 49 692.0 0 49 103 55 692.0 0 55 104 35 692.0 0 35 105 38 692.0 0 38 106 56 692.0 0 56 107 63 692.0 0 63 108 37 692.0 0 37 109 59 692.0 0 59 110 50 692.0 0 50 111 71 692.0 0 71 112 104 692.0 0 104 113 1735 692.0 0 1735 114 944 692.0 0 944 115 1368 692.0 0 1368 116 54 692.0 0 54 117 37 692.0 0 37 118 30 692.0 0 30 119 29 692.0 0 29 120 49 692.0 0 49 121 44 692.0 0 44 122 63 692.0 0 63 123 44 692.0 0 44 124 38 692.0 0 38 125 36 692.0 0 36 126 26 692.0 0 26 127 23 692.0 0 23 128 69 692.0 0 69 129 36 692.0 0 36 130 69 692.0 0 69 131 32 692.0 0 32 132 63 692.0 0 63 133 69 692.0 0 69 134 140 692.0 0 140 135 70 692.0 0 70 136 58 692.0 0 58 137 111 692.0 0 111 138 62 692.0 0 62 139 48 692.0 0 48 140 63 692.0 0 63 141 83 692.0 0 83 142 132 692.0 0 132 143 102 692.0 0 102 144 161 692.0 0 161 145 131 692.0 0 131 146 161 692.0 0 161 147 1283 692.0 0 1283 148 1281 692.0 0 1281 149 5748 692.0 0 5748 150 427 692.0 0 427 151 40 692.0 0 40 152 51 692.0 0 51 153 40 692.0 0 40 154 41 692.0 0 41 155 48 692.0 0 48 156 55 692.0 0 55 157 38 692.0 0 38 158 44 692.0 0 44 159 45 692.0 0 45 160 46 692.0 0 46 161 40 692.0 0 40 162 67 692.0 0 67 163 52 692.0 0 52 164 42 692.0 0 42 165 48 692.0 0 48 166 26 692.0 0 26 167 40 692.0 0 40 168 85 692.0 0 85 169 62 692.0 0 62 170 70 692.0 0 70 171 38 692.0 0 38 172 60 692.0 0 60 173 57 692.0 0 57 174 65 692.0 0 65 175 57 692.0 0 57 176 80 692.0 0 80 177 72 692.0 0 72 178 100 692.0 0 100 179 129 692.0 0 129 180 95 692.0 0 95 181 181 692.0 0 181 182 135 692.0 0 135 183 135 692.0 0 135 184 126 692.0 0 126 185 207 692.0 0 207 186 296 692.0 0 296 187 456 692.0 0 456 188 565 692.0 0 565 189 6759 692.0 0 6759 190 1429 692.0 0 1429 191 2092 692.0 0 2092 192 1353 692.0 0 1353 193 812 692.0 0 812 194 631 692.0 0 631 195 786 692.0 0 786 196 745 692.0 0 745 197 688 692.0 0 688 198 916 692.0 0 916 199 1613 692.0 0 1613 200 4277 692.0 0 4277 201 6857 692.0 0 6857 202 17797 692.0 0 17797 203 2051 692.0 0 2051 204 2153 692.0 0 2153 205 35275 692.0 0 35275 206 19631 692.0 0 19631 207 44175 692.0 0 44175 208 20392 692.0 0 20392 209 4678 692.0 0 4678 210 1297 692.0 0 1297 211 2451 692.0 0 2451 212 1531 692.0 0 1531 213 6220 692.0 0 6220 214 2013 692.0 0 2013 215 959 692.0 0 959 216 743 692.0 0 743 217 790 692.0 0 790 218 728 692.0 0 728 219 1429 692.0 0 1429 220 2208 692.0 0 2208 221 3386 692.0 0 3386 222 2009 692.0 0 2009 223 1670 692.0 0 1670 224 1413 692.0 0 1413 225 10201 692.0 0 10201 226 23969 692.0 0 23969 227 32145 692.0 0 32145 228 12119 692.0 0 12119 229 77 692.0 0 77 230 40 692.0 0 40 231 42 692.0 0 42 232 29 692.0 0 29 233 32 692.0 0 32 234 56 692.0 0 56 235 57 692.0 0 57 236 83 692.0 0 83 237 67 692.0 0 67 238 90 692.0 0 90 239 46 692.0 0 46 240 56 692.0 0 56 241 66 692.0 0 66 242 66 692.0 0 66 243 70 692.0 0 70 244 346 692.0 0 346 245 328 692.0 0 328 246 897 692.0 0 897 247 270 692.0 0 270 248 550 692.0 0 550 249 749 692.0 0 749 250 266 692.0 0 266 251 320 692.0 0 320 252 434 692.0 0 434 253 253 692.0 0 253 254 175 692.0 0 175 255 389 692.0 0 389 256 239 692.0 0 239 257 330 692.0 0 330 258 432 692.0 0 432 259 1596 692.0 0 1596 260 1342 692.0 0 1342 261 1349 692.0 0 1349 262 18766 692.0 0 18766 263 7883 692.0 0 7883 264 17568 692.0 0 17568 265 388 692.0 0 388 266 179 692.0 0 179 267 40 692.0 0 40 268 44 692.0 0 44 269 112 692.0 0 112 270 200 692.0 0 200 271 247 692.0 0 247 272 97 692.0 0 97 273 101 692.0 0 101 274 64 692.0 0 64 275 81 692.0 0 81 276 72 692.0 0 72 277 60 692.0 0 60 278 165 692.0 0 165 279 69 692.0 0 69 280 56 692.0 0 56 281 60 692.0 0 60 282 67 692.0 0 67 283 173 692.0 0 173 284 196 692.0 0 196 285 140 692.0 0 140 286 104 692.0 0 104 287 75 692.0 0 75 288 55 692.0 0 55 289 89 692.0 0 89 290 64 692.0 0 64 291 82 692.0 0 82 292 952 692.0 0 952 293 508 692.0 0 508 294 1109 692.0 0 1109 295 224 692.0 0 224 296 267 692.0 0 267 297 155 692.0 0 155 298 316 692.0 0 316 299 93 692.0 0 93 300 140 692.0 0 140 301 152 692.0 0 152 Finished in 15.21 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 309,623 Reads with adapters: 97,329 (31.4%) Reads that were too short: 105 (0.0%) Reads written (passing filters): 97,224 (31.4%) Total basepairs processed: 82,441,420 bp Total written (filtered): 26,642,697 bp (32.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 97329 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 7 0.0 3 0 0 0 3 4 18 63 0.0 3 0 0 46 14 3 19 1465 0.0 3 0 1284 110 51 20 20 95090 0.0 4 89362 4623 807 229 69 21 305 0.0 4 84 195 20 4 2 22 21 0.0 4 6 1 7 3 4 23 196 0.0 4 0 0 0 146 50 24 5 0.0 4 1 0 0 1 3 26 1 0.0 4 0 0 0 0 1 27 13 0.0 4 0 1 0 0 12 28 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 61 1 0.0 4 0 1 64 4 0.0 4 0 0 0 0 4 65 1 0.0 4 0 0 0 0 1 66 3 0.0 4 0 0 0 0 3 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 2 0.0 4 1 0 0 0 1 86 1 0.0 4 0 0 0 0 1 92 10 0.0 4 0 0 0 1 9 95 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 1 102 1 0.0 4 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 1 113 1 0.0 4 1 118 1 0.0 4 1 119 3 0.0 4 0 0 0 0 3 120 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 1 0 0 1 127 13 0.0 4 1 0 0 0 12 128 2 0.0 4 1 0 0 0 1 131 1 0.0 4 0 0 0 0 1 136 2 0.0 4 1 1 141 1 0.0 4 0 0 1 145 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 1 152 1 0.0 4 0 0 0 0 1 153 10 0.0 4 0 0 0 0 10 156 2 0.0 4 2 160 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 180 1 0.0 4 1 181 1 0.0 4 1 185 1 0.0 4 0 0 1 187 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 3 0.0 4 0 0 0 0 3 219 3 0.0 4 2 1 220 1 0.0 4 0 0 0 1 222 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 1 225 2 0.0 4 0 0 1 0 1 226 1 0.0 4 1 227 2 0.0 4 1 1 228 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 1 233 5 0.0 4 0 0 0 0 5 234 7 0.0 4 0 0 0 0 7 235 4 0.0 4 0 0 0 0 4 239 3 0.0 4 0 0 0 1 2 242 1 0.0 4 0 0 1 243 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 249 3 0.0 4 0 0 0 0 3 251 2 0.0 4 1 0 0 0 1 252 2 0.0 4 0 0 1 0 1 253 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 4 0.0 4 0 0 0 0 4 258 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 1 265 1 0.0 4 0 1 266 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 1 287 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 1 290 2 0.0 4 0 0 0 0 2 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.33 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 97,224 Reads with adapters: 3,537 (3.6%) Reads written (passing filters): 97,224 (100.0%) Total basepairs processed: 26,642,697 bp Total written (filtered): 26,278,489 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3537 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 55.8% C: 12.9% G: 28.5% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 516 1519.1 0 516 4 50 379.8 0 50 5 6 94.9 0 6 9 23 0.4 0 2 21 10 36 0.1 1 0 36 11 153 0.0 1 11 142 12 977 0.0 1 41 936 13 64 0.0 1 3 61 15 19 0.0 1 0 19 16 2 0.0 1 0 2 20 1 0.0 1 1 21 12 0.0 1 11 1 22 1 0.0 1 1 24 10 0.0 1 7 3 25 2 0.0 1 2 38 3 0.0 1 2 1 39 1 0.0 1 1 65 1 0.0 1 0 1 70 4 0.0 1 3 1 71 1 0.0 1 1 73 2 0.0 1 1 1 85 1 0.0 1 1 93 2 0.0 1 1 1 101 1 0.0 1 1 108 1 0.0 1 1 116 2 0.0 1 2 126 1 0.0 1 0 1 127 1 0.0 1 1 140 2 0.0 1 2 152 14 0.0 1 10 4 153 1 0.0 1 1 158 6 0.0 1 4 2 159 2 0.0 1 2 165 1 0.0 1 1 168 2 0.0 1 1 1 169 1 0.0 1 1 178 1 0.0 1 1 191 7 0.0 1 1 6 192 3 0.0 1 0 3 193 5 0.0 1 0 5 196 2 0.0 1 2 207 1 0.0 1 1 208 1 0.0 1 1 209 8 0.0 1 4 4 210 1317 0.0 1 1023 294 211 181 0.0 1 143 38 212 3 0.0 1 2 1 213 1 0.0 1 1 215 10 0.0 1 10 225 1 0.0 1 1 228 2 0.0 1 2 229 2 0.0 1 2 230 64 0.0 1 55 9 231 5 0.0 1 3 2 237 1 0.0 1 1