This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.75 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 654,903 (23.1%) Reads that were too short: 330,265 (11.7%) Reads written (passing filters): 324,638 (11.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 85,815,074 bp (10.1%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 654903 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 16832 692.0 0 16832 7 126407 692.0 0 126407 8 3983 692.0 0 3983 9 120206 692.0 0 120206 10 122 692.0 0 122 11 40 692.0 0 40 12 18 692.0 0 18 13 35 692.0 0 35 14 26 692.0 0 26 15 22 692.0 0 22 16 20 692.0 0 20 17 22 692.0 0 22 18 15 692.0 0 15 19 33 692.0 0 33 20 38 692.0 0 38 21 28 692.0 0 28 22 21 692.0 0 21 23 19 692.0 0 19 24 18 692.0 0 18 25 25 692.0 0 25 26 14 692.0 0 14 27 23 692.0 0 23 28 15 692.0 0 15 29 25 692.0 0 25 30 26 692.0 0 26 31 49 692.0 0 49 32 62 692.0 0 62 33 32 692.0 0 32 34 23 692.0 0 23 35 32 692.0 0 32 36 35 692.0 0 35 37 25 692.0 0 25 38 20 692.0 0 20 39 35 692.0 0 35 40 20 692.0 0 20 41 39 692.0 0 39 42 26 692.0 0 26 43 34 692.0 0 34 44 29 692.0 0 29 45 37 692.0 0 37 46 28 692.0 0 28 47 26 692.0 0 26 48 20 692.0 0 20 49 21 692.0 0 21 50 18 692.0 0 18 51 18 692.0 0 18 52 20 692.0 0 20 53 18 692.0 0 18 54 28 692.0 0 28 55 38 692.0 0 38 56 30 692.0 0 30 57 49 692.0 0 49 58 50 692.0 0 50 59 60 692.0 0 60 60 34 692.0 0 34 61 28 692.0 0 28 62 23 692.0 0 23 63 27 692.0 0 27 64 24 692.0 0 24 65 69 692.0 0 69 66 36 692.0 0 36 67 26 692.0 0 26 68 24 692.0 0 24 69 18 692.0 0 18 70 24 692.0 0 24 71 25 692.0 0 25 72 24 692.0 0 24 73 31 692.0 0 31 74 29 692.0 0 29 75 78 692.0 0 78 76 107 692.0 0 107 77 73 692.0 0 73 78 36 692.0 0 36 79 24 692.0 0 24 80 24 692.0 0 24 81 28 692.0 0 28 82 24 692.0 0 24 83 21 692.0 0 21 84 21 692.0 0 21 85 28 692.0 0 28 86 16 692.0 0 16 87 30 692.0 0 30 88 28 692.0 0 28 89 42 692.0 0 42 90 25 692.0 0 25 91 41 692.0 0 41 92 37 692.0 0 37 93 40 692.0 0 40 94 37 692.0 0 37 95 42 692.0 0 42 96 27 692.0 0 27 97 28 692.0 0 28 98 41 692.0 0 41 99 36 692.0 0 36 100 66 692.0 0 66 101 43 692.0 0 43 102 47 692.0 0 47 103 65 692.0 0 65 104 49 692.0 0 49 105 32 692.0 0 32 106 53 692.0 0 53 107 51 692.0 0 51 108 38 692.0 0 38 109 41 692.0 0 41 110 54 692.0 0 54 111 53 692.0 0 53 112 64 692.0 0 64 113 1364 692.0 0 1364 114 782 692.0 0 782 115 1045 692.0 0 1045 116 41 692.0 0 41 117 34 692.0 0 34 118 26 692.0 0 26 119 21 692.0 0 21 120 37 692.0 0 37 121 47 692.0 0 47 122 45 692.0 0 45 123 57 692.0 0 57 124 42 692.0 0 42 125 41 692.0 0 41 126 35 692.0 0 35 127 21 692.0 0 21 128 63 692.0 0 63 129 34 692.0 0 34 130 76 692.0 0 76 131 37 692.0 0 37 132 35 692.0 0 35 133 81 692.0 0 81 134 141 692.0 0 141 135 76 692.0 0 76 136 61 692.0 0 61 137 124 692.0 0 124 138 69 692.0 0 69 139 44 692.0 0 44 140 59 692.0 0 59 141 79 692.0 0 79 142 133 692.0 0 133 143 114 692.0 0 114 144 175 692.0 0 175 145 109 692.0 0 109 146 124 692.0 0 124 147 1296 692.0 0 1296 148 1231 692.0 0 1231 149 6264 692.0 0 6264 150 428 692.0 0 428 151 22 692.0 0 22 152 33 692.0 0 33 153 26 692.0 0 26 154 36 692.0 0 36 155 60 692.0 0 60 156 39 692.0 0 39 157 44 692.0 0 44 158 42 692.0 0 42 159 43 692.0 0 43 160 44 692.0 0 44 161 58 692.0 0 58 162 93 692.0 0 93 163 68 692.0 0 68 164 36 692.0 0 36 165 63 692.0 0 63 166 35 692.0 0 35 167 30 692.0 0 30 168 68 692.0 0 68 169 48 692.0 0 48 170 46 692.0 0 46 171 41 692.0 0 41 172 64 692.0 0 64 173 46 692.0 0 46 174 73 692.0 0 73 175 52 692.0 0 52 176 76 692.0 0 76 177 68 692.0 0 68 178 53 692.0 0 53 179 138 692.0 0 138 180 98 692.0 0 98 181 197 692.0 0 197 182 151 692.0 0 151 183 151 692.0 0 151 184 140 692.0 0 140 185 211 692.0 0 211 186 316 692.0 0 316 187 645 692.0 0 645 188 689 692.0 0 689 189 10536 692.0 0 10536 190 1805 692.0 0 1805 191 2917 692.0 0 2917 192 1548 692.0 0 1548 193 928 692.0 0 928 194 631 692.0 0 631 195 845 692.0 0 845 196 743 692.0 0 743 197 626 692.0 0 626 198 861 692.0 0 861 199 1613 692.0 0 1613 200 4470 692.0 0 4470 201 7494 692.0 0 7494 202 19627 692.0 0 19627 203 1951 692.0 0 1951 204 2084 692.0 0 2084 205 34995 692.0 0 34995 206 23174 692.0 0 23174 207 49949 692.0 0 49949 208 24128 692.0 0 24128 209 4445 692.0 0 4445 210 1508 692.0 0 1508 211 2977 692.0 0 2977 212 1654 692.0 0 1654 213 7607 692.0 0 7607 214 2362 692.0 0 2362 215 1015 692.0 0 1015 216 778 692.0 0 778 217 1031 692.0 0 1031 218 789 692.0 0 789 219 1913 692.0 0 1913 220 2758 692.0 0 2758 221 4461 692.0 0 4461 222 2381 692.0 0 2381 223 1863 692.0 0 1863 224 1296 692.0 0 1296 225 9075 692.0 0 9075 226 26948 692.0 0 26948 227 34005 692.0 0 34005 228 16008 692.0 0 16008 229 79 692.0 0 79 230 35 692.0 0 35 231 24 692.0 0 24 232 32 692.0 0 32 233 21 692.0 0 21 234 30 692.0 0 30 235 18 692.0 0 18 236 60 692.0 0 60 237 43 692.0 0 43 238 64 692.0 0 64 239 31 692.0 0 31 240 48 692.0 0 48 241 36 692.0 0 36 242 30 692.0 0 30 243 29 692.0 0 29 244 144 692.0 0 144 245 145 692.0 0 145 246 311 692.0 0 311 247 177 692.0 0 177 248 424 692.0 0 424 249 538 692.0 0 538 250 132 692.0 0 132 251 192 692.0 0 192 252 288 692.0 0 288 253 156 692.0 0 156 254 130 692.0 0 130 255 349 692.0 0 349 256 209 692.0 0 209 257 190 692.0 0 190 258 244 692.0 0 244 259 777 692.0 0 777 260 839 692.0 0 839 261 800 692.0 0 800 262 17733 692.0 0 17733 263 6841 692.0 0 6841 264 16019 692.0 0 16019 265 168 692.0 0 168 266 122 692.0 0 122 267 18 692.0 0 18 268 26 692.0 0 26 269 32 692.0 0 32 270 54 692.0 0 54 271 80 692.0 0 80 272 75 692.0 0 75 273 86 692.0 0 86 274 98 692.0 0 98 275 24 692.0 0 24 276 28 692.0 0 28 277 35 692.0 0 35 278 143 692.0 0 143 279 25 692.0 0 25 280 26 692.0 0 26 281 5 692.0 0 5 282 17 692.0 0 17 283 40 692.0 0 40 284 53 692.0 0 53 285 30 692.0 0 30 286 21 692.0 0 21 287 16 692.0 0 16 288 30 692.0 0 30 289 22 692.0 0 22 290 18 692.0 0 18 291 23 692.0 0 23 292 194 692.0 0 194 293 97 692.0 0 97 294 264 692.0 0 264 295 51 692.0 0 51 296 107 692.0 0 107 297 43 692.0 0 43 298 79 692.0 0 79 299 31 692.0 0 31 300 32 692.0 0 32 301 52 692.0 0 52 Finished in 14.08 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 324,638 Reads with adapters: 125,479 (38.7%) Reads that were too short: 77 (0.0%) Reads written (passing filters): 125,402 (38.6%) Total basepairs processed: 85,815,074 bp Total written (filtered): 34,475,633 bp (40.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 125479 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 24 0.0 3 0 0 23 1 16 1028 0.0 3 0 996 31 1 17 123557 0.0 3 121221 2252 75 9 18 329 0.0 3 57 245 24 3 19 36 0.0 3 1 0 27 8 20 316 0.0 3 1 0 1 314 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 1 26 6 0.0 3 0 0 0 6 32 3 0.0 3 3 33 1 0.0 3 0 0 0 1 34 2 0.0 3 1 0 0 1 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 75 27 0.0 3 0 0 0 27 77 1 0.0 3 1 78 35 0.0 3 0 0 0 35 79 2 0.0 3 0 0 0 2 80 1 0.0 3 1 81 11 0.0 3 0 0 0 11 91 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 1 96 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 118 1 0.0 3 1 126 1 0.0 3 0 0 0 1 130 2 0.0 3 0 1 0 1 133 1 0.0 3 1 137 2 0.0 3 0 0 1 1 140 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 179 2 0.0 3 1 0 0 1 180 1 0.0 3 0 0 1 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 187 1 0.0 3 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 24 0.0 3 0 0 0 24 235 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 1 246 1 0.0 3 1 249 1 0.0 3 1 263 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 7 0.0 3 0 0 1 6 282 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.47 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 125,402 Reads with adapters: 520 (0.4%) Reads written (passing filters): 125,402 (100.0%) Total basepairs processed: 34,475,633 bp Total written (filtered): 34,473,934 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 520 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.1% C: 22.3% G: 18.8% T: 11.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 436 1959.4 0 436 4 52 489.9 0 52 5 14 122.5 0 14 6 14 30.6 0 14 7 3 7.7 0 3 8 1 1.9 0 1