This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.31 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 172,315 (6.1%) Reads that were too short: 33,101 (1.2%) Reads written (passing filters): 139,214 (4.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 36,452,285 bp (4.3%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 172315 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1084 692.0 0 1084 7 6716 692.0 0 6716 8 54291 692.0 0 54291 9 42671 692.0 0 42671 10 78 692.0 0 78 11 18 692.0 0 18 12 23 692.0 0 23 13 18 692.0 0 18 14 18 692.0 0 18 15 16 692.0 0 16 16 17 692.0 0 17 17 23 692.0 0 23 18 21 692.0 0 21 19 12 692.0 0 12 20 22 692.0 0 22 21 10 692.0 0 10 22 12 692.0 0 12 23 20 692.0 0 20 24 21 692.0 0 21 25 36 692.0 0 36 26 16 692.0 0 16 27 30 692.0 0 30 28 19 692.0 0 19 29 18 692.0 0 18 30 21 692.0 0 21 31 18 692.0 0 18 32 68 692.0 0 68 33 48 692.0 0 48 34 43 692.0 0 43 35 29 692.0 0 29 36 52 692.0 0 52 37 192 692.0 0 192 38 165 692.0 0 165 39 185 692.0 0 185 40 83 692.0 0 83 41 54 692.0 0 54 42 526 692.0 0 526 43 14 692.0 0 14 44 31 692.0 0 31 45 17 692.0 0 17 46 31 692.0 0 31 47 30 692.0 0 30 48 24 692.0 0 24 49 33 692.0 0 33 50 23 692.0 0 23 51 39 692.0 0 39 52 416 692.0 0 416 53 140 692.0 0 140 54 217 692.0 0 217 55 27 692.0 0 27 56 13 692.0 0 13 57 21 692.0 0 21 58 20 692.0 0 20 59 23 692.0 0 23 60 44 692.0 0 44 61 64 692.0 0 64 62 56 692.0 0 56 63 18 692.0 0 18 64 19 692.0 0 19 65 39 692.0 0 39 66 77 692.0 0 77 67 30 692.0 0 30 68 92 692.0 0 92 69 16 692.0 0 16 70 17 692.0 0 17 71 54 692.0 0 54 72 83 692.0 0 83 73 133 692.0 0 133 74 15 692.0 0 15 75 19 692.0 0 19 76 12 692.0 0 12 77 24 692.0 0 24 78 12 692.0 0 12 79 10 692.0 0 10 80 15 692.0 0 15 81 30 692.0 0 30 82 19 692.0 0 19 83 18 692.0 0 18 84 88 692.0 0 88 85 90 692.0 0 90 86 114 692.0 0 114 87 36 692.0 0 36 88 21 692.0 0 21 89 18 692.0 0 18 90 85 692.0 0 85 91 81 692.0 0 81 92 74 692.0 0 74 93 137 692.0 0 137 94 98 692.0 0 98 95 165 692.0 0 165 96 57 692.0 0 57 97 62 692.0 0 62 98 32 692.0 0 32 99 17 692.0 0 17 100 20 692.0 0 20 101 17 692.0 0 17 102 19 692.0 0 19 103 24 692.0 0 24 104 75 692.0 0 75 105 76 692.0 0 76 106 60 692.0 0 60 107 70 692.0 0 70 108 217 692.0 0 217 109 269 692.0 0 269 110 222 692.0 0 222 111 112 692.0 0 112 112 200 692.0 0 200 113 289 692.0 0 289 114 30 692.0 0 30 115 41 692.0 0 41 116 32 692.0 0 32 117 98 692.0 0 98 118 72 692.0 0 72 119 94 692.0 0 94 120 25 692.0 0 25 121 23 692.0 0 23 122 26 692.0 0 26 123 33 692.0 0 33 124 46 692.0 0 46 125 68 692.0 0 68 126 97 692.0 0 97 127 566 692.0 0 566 128 113 692.0 0 113 129 302 692.0 0 302 130 37 692.0 0 37 131 39 692.0 0 39 132 60 692.0 0 60 133 91 692.0 0 91 134 41 692.0 0 41 135 29 692.0 0 29 136 39 692.0 0 39 137 42 692.0 0 42 138 61 692.0 0 61 139 124 692.0 0 124 140 101 692.0 0 101 141 142 692.0 0 142 142 294 692.0 0 294 143 447 692.0 0 447 144 7371 692.0 0 7371 145 3664 692.0 0 3664 146 8068 692.0 0 8068 147 83 692.0 0 83 148 45 692.0 0 45 149 37 692.0 0 37 150 89 692.0 0 89 151 562 692.0 0 562 152 305 692.0 0 305 153 261 692.0 0 261 154 126 692.0 0 126 155 68 692.0 0 68 156 195 692.0 0 195 157 128 692.0 0 128 158 167 692.0 0 167 159 63 692.0 0 63 160 36 692.0 0 36 161 80 692.0 0 80 162 55 692.0 0 55 163 25 692.0 0 25 164 31 692.0 0 31 165 37 692.0 0 37 166 26 692.0 0 26 167 37 692.0 0 37 168 20 692.0 0 20 169 21 692.0 0 21 170 41 692.0 0 41 171 84 692.0 0 84 172 111 692.0 0 111 173 92 692.0 0 92 174 38 692.0 0 38 175 31 692.0 0 31 176 34 692.0 0 34 177 94 692.0 0 94 178 55 692.0 0 55 179 705 692.0 0 705 180 104 692.0 0 104 181 153 692.0 0 153 182 30 692.0 0 30 183 32 692.0 0 32 184 36 692.0 0 36 185 52 692.0 0 52 186 78 692.0 0 78 187 33 692.0 0 33 188 32 692.0 0 32 189 25 692.0 0 25 190 99 692.0 0 99 191 29 692.0 0 29 192 162 692.0 0 162 193 47 692.0 0 47 194 36 692.0 0 36 195 45 692.0 0 45 196 37 692.0 0 37 197 73 692.0 0 73 198 223 692.0 0 223 199 65 692.0 0 65 200 83 692.0 0 83 201 68 692.0 0 68 202 31 692.0 0 31 203 28 692.0 0 28 204 36 692.0 0 36 205 93 692.0 0 93 206 70 692.0 0 70 207 45 692.0 0 45 208 30 692.0 0 30 209 21 692.0 0 21 210 15 692.0 0 15 211 33 692.0 0 33 212 40 692.0 0 40 213 29 692.0 0 29 214 39 692.0 0 39 215 30 692.0 0 30 216 35 692.0 0 35 217 130 692.0 0 130 218 70 692.0 0 70 219 66 692.0 0 66 220 30 692.0 0 30 221 25 692.0 0 25 222 32 692.0 0 32 223 30 692.0 0 30 224 25 692.0 0 25 225 91 692.0 0 91 226 56 692.0 0 56 227 60 692.0 0 60 228 164 692.0 0 164 229 94 692.0 0 94 230 196 692.0 0 196 231 91 692.0 0 91 232 75 692.0 0 75 233 91 692.0 0 91 234 81 692.0 0 81 235 280 692.0 0 280 236 961 692.0 0 961 237 445 692.0 0 445 238 467 692.0 0 467 239 127 692.0 0 127 240 726 692.0 0 726 241 256 692.0 0 256 242 237 692.0 0 237 243 69 692.0 0 69 244 56 692.0 0 56 245 110 692.0 0 110 246 123 692.0 0 123 247 336 692.0 0 336 248 139 692.0 0 139 249 159 692.0 0 159 250 378 692.0 0 378 251 261 692.0 0 261 252 623 692.0 0 623 253 156 692.0 0 156 254 64 692.0 0 64 255 79 692.0 0 79 256 129 692.0 0 129 257 131 692.0 0 131 258 215 692.0 0 215 259 378 692.0 0 378 260 5047 692.0 0 5047 261 5340 692.0 0 5340 262 4103 692.0 0 4103 263 655 692.0 0 655 264 50 692.0 0 50 265 55 692.0 0 55 266 102 692.0 0 102 267 155 692.0 0 155 268 160 692.0 0 160 269 82 692.0 0 82 270 444 692.0 0 444 271 123 692.0 0 123 272 522 692.0 0 522 273 72 692.0 0 72 274 45 692.0 0 45 275 54 692.0 0 54 276 94 692.0 0 94 277 137 692.0 0 137 278 163 692.0 0 163 279 183 692.0 0 183 280 352 692.0 0 352 281 267 692.0 0 267 282 274 692.0 0 274 283 120 692.0 0 120 284 167 692.0 0 167 285 642 692.0 0 642 286 117 692.0 0 117 287 121 692.0 0 121 288 591 692.0 0 591 289 187 692.0 0 187 290 300 692.0 0 300 291 621 692.0 0 621 292 509 692.0 0 509 293 773 692.0 0 773 294 669 692.0 0 669 295 326 692.0 0 326 296 330 692.0 0 330 297 57 692.0 0 57 298 47 692.0 0 47 299 43 692.0 0 43 300 52 692.0 0 52 301 63 692.0 0 63 Finished in 6.98 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 139,214 Reads with adapters: 45,048 (32.4%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 45,016 (32.3%) Total basepairs processed: 36,452,285 bp Total written (filtered): 12,235,982 bp (33.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45048 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 7 3 18 37 0.0 3 0 0 21 10 6 19 724 0.0 3 0 603 48 55 18 20 43560 0.0 4 40474 2417 483 145 41 21 118 0.0 4 16 90 8 3 1 22 24 0.0 4 6 5 8 0 5 23 436 0.0 4 66 4 2 275 89 24 4 0.0 4 0 0 0 0 4 25 1 0.0 4 0 0 1 26 3 0.0 4 0 0 0 2 1 27 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 1 30 1 0.0 4 0 0 0 0 1 43 1 0.0 4 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 116 1 0.0 4 1 126 1 0.0 4 0 0 0 0 1 127 79 0.0 4 0 0 0 0 79 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 8 0.0 4 0 0 0 0 8 185 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 233 3 0.0 4 0 0 0 0 3 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.64 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 45,016 Reads with adapters: 11,104 (24.7%) Reads written (passing filters): 45,016 (100.0%) Total basepairs processed: 12,235,982 bp Total written (filtered): 12,065,332 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11104 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 8.9% G: 83.8% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 18 703.4 0 18 4 5 175.8 0 5 6 2 11.0 0 2 7 1 2.7 0 1 8 1 0.7 0 1 9 466 0.2 0 55 411 10 9700 0.0 1 772 8928 11 564 0.0 1 20 544 12 12 0.0 1 0 12 13 24 0.0 1 0 24 14 1 0.0 1 0 1 16 3 0.0 1 2 1 18 1 0.0 1 1 23 4 0.0 1 1 3 75 1 0.0 1 1 150 4 0.0 1 2 2 189 1 0.0 1 0 1 207 1 0.0 1 0 1 208 280 0.0 1 211 69 209 10 0.0 1 8 2 210 1 0.0 1 1 228 4 0.0 1 3 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.