This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.22 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 154,802 (5.5%) Reads that were too short: 16,035 (0.6%) Reads written (passing filters): 138,767 (4.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 36,693,434 bp (4.3%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 154802 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 538 692.0 0 538 7 3790 692.0 0 3790 8 43991 692.0 0 43991 9 60196 692.0 0 60196 10 64 692.0 0 64 11 15 692.0 0 15 12 6 692.0 0 6 13 7 692.0 0 7 14 5 692.0 0 5 15 5 692.0 0 5 16 12 692.0 0 12 17 7 692.0 0 7 18 8 692.0 0 8 19 3 692.0 0 3 20 9 692.0 0 9 21 3 692.0 0 3 22 12 692.0 0 12 23 10 692.0 0 10 24 13 692.0 0 13 25 9 692.0 0 9 26 6 692.0 0 6 27 10 692.0 0 10 28 15 692.0 0 15 29 9 692.0 0 9 30 10 692.0 0 10 31 13 692.0 0 13 32 26 692.0 0 26 33 18 692.0 0 18 34 29 692.0 0 29 35 19 692.0 0 19 36 18 692.0 0 18 37 159 692.0 0 159 38 87 692.0 0 87 39 94 692.0 0 94 40 12 692.0 0 12 41 12 692.0 0 12 42 118 692.0 0 118 43 6 692.0 0 6 44 10 692.0 0 10 45 7 692.0 0 7 46 8 692.0 0 8 47 10 692.0 0 10 48 11 692.0 0 11 49 18 692.0 0 18 50 13 692.0 0 13 51 25 692.0 0 25 52 177 692.0 0 177 53 128 692.0 0 128 54 95 692.0 0 95 55 40 692.0 0 40 56 6 692.0 0 6 57 20 692.0 0 20 58 8 692.0 0 8 59 10 692.0 0 10 60 330 692.0 0 330 61 87 692.0 0 87 62 24 692.0 0 24 63 7 692.0 0 7 64 13 692.0 0 13 65 8 692.0 0 8 66 19 692.0 0 19 67 12 692.0 0 12 68 16 692.0 0 16 69 10 692.0 0 10 70 7 692.0 0 7 71 23 692.0 0 23 72 28 692.0 0 28 73 110 692.0 0 110 74 6 692.0 0 6 75 9 692.0 0 9 76 9 692.0 0 9 77 6 692.0 0 6 78 8 692.0 0 8 79 12 692.0 0 12 80 16 692.0 0 16 81 20 692.0 0 20 82 21 692.0 0 21 83 13 692.0 0 13 84 49 692.0 0 49 85 29 692.0 0 29 86 51 692.0 0 51 87 17 692.0 0 17 88 11 692.0 0 11 89 14 692.0 0 14 90 49 692.0 0 49 91 32 692.0 0 32 92 24 692.0 0 24 93 26 692.0 0 26 94 26 692.0 0 26 95 47 692.0 0 47 96 40 692.0 0 40 97 47 692.0 0 47 98 16 692.0 0 16 99 12 692.0 0 12 100 10 692.0 0 10 101 10 692.0 0 10 102 11 692.0 0 11 103 24 692.0 0 24 104 47 692.0 0 47 105 57 692.0 0 57 106 45 692.0 0 45 107 42 692.0 0 42 108 70 692.0 0 70 109 70 692.0 0 70 110 71 692.0 0 71 111 32 692.0 0 32 112 64 692.0 0 64 113 69 692.0 0 69 114 32 692.0 0 32 115 21 692.0 0 21 116 19 692.0 0 19 117 91 692.0 0 91 118 30 692.0 0 30 119 25 692.0 0 25 120 10 692.0 0 10 121 18 692.0 0 18 122 14 692.0 0 14 123 11 692.0 0 11 124 31 692.0 0 31 125 38 692.0 0 38 126 70 692.0 0 70 127 530 692.0 0 530 128 115 692.0 0 115 129 189 692.0 0 189 130 33 692.0 0 33 131 29 692.0 0 29 132 53 692.0 0 53 133 76 692.0 0 76 134 33 692.0 0 33 135 31 692.0 0 31 136 34 692.0 0 34 137 45 692.0 0 45 138 56 692.0 0 56 139 75 692.0 0 75 140 99 692.0 0 99 141 131 692.0 0 131 142 368 692.0 0 368 143 481 692.0 0 481 144 8504 692.0 0 8504 145 4296 692.0 0 4296 146 9081 692.0 0 9081 147 73 692.0 0 73 148 36 692.0 0 36 149 13 692.0 0 13 150 33 692.0 0 33 151 167 692.0 0 167 152 66 692.0 0 66 153 71 692.0 0 71 154 42 692.0 0 42 155 18 692.0 0 18 156 63 692.0 0 63 157 52 692.0 0 52 158 84 692.0 0 84 159 19 692.0 0 19 160 22 692.0 0 22 161 29 692.0 0 29 162 45 692.0 0 45 163 14 692.0 0 14 164 15 692.0 0 15 165 20 692.0 0 20 166 16 692.0 0 16 167 22 692.0 0 22 168 13 692.0 0 13 169 19 692.0 0 19 170 14 692.0 0 14 171 25 692.0 0 25 172 40 692.0 0 40 173 34 692.0 0 34 174 14 692.0 0 14 175 15 692.0 0 15 176 25 692.0 0 25 177 35 692.0 0 35 178 41 692.0 0 41 179 286 692.0 0 286 180 63 692.0 0 63 181 76 692.0 0 76 182 25 692.0 0 25 183 8 692.0 0 8 184 19 692.0 0 19 185 12 692.0 0 12 186 18 692.0 0 18 187 9 692.0 0 9 188 12 692.0 0 12 189 13 692.0 0 13 190 65 692.0 0 65 191 26 692.0 0 26 192 99 692.0 0 99 193 24 692.0 0 24 194 15 692.0 0 15 195 16 692.0 0 16 196 21 692.0 0 21 197 32 692.0 0 32 198 111 692.0 0 111 199 30 692.0 0 30 200 39 692.0 0 39 201 8 692.0 0 8 202 12 692.0 0 12 203 15 692.0 0 15 204 15 692.0 0 15 205 24 692.0 0 24 206 24 692.0 0 24 207 13 692.0 0 13 208 11 692.0 0 11 209 10 692.0 0 10 210 7 692.0 0 7 211 11 692.0 0 11 212 23 692.0 0 23 213 15 692.0 0 15 214 23 692.0 0 23 215 16 692.0 0 16 216 26 692.0 0 26 217 172 692.0 0 172 218 50 692.0 0 50 219 30 692.0 0 30 220 14 692.0 0 14 221 8 692.0 0 8 222 13 692.0 0 13 223 23 692.0 0 23 224 25 692.0 0 25 225 37 692.0 0 37 226 35 692.0 0 35 227 35 692.0 0 35 228 54 692.0 0 54 229 58 692.0 0 58 230 136 692.0 0 136 231 45 692.0 0 45 232 63 692.0 0 63 233 38 692.0 0 38 234 36 692.0 0 36 235 142 692.0 0 142 236 406 692.0 0 406 237 173 692.0 0 173 238 162 692.0 0 162 239 50 692.0 0 50 240 344 692.0 0 344 241 135 692.0 0 135 242 140 692.0 0 140 243 34 692.0 0 34 244 24 692.0 0 24 245 51 692.0 0 51 246 46 692.0 0 46 247 160 692.0 0 160 248 66 692.0 0 66 249 50 692.0 0 50 250 124 692.0 0 124 251 131 692.0 0 131 252 223 692.0 0 223 253 63 692.0 0 63 254 25 692.0 0 25 255 30 692.0 0 30 256 53 692.0 0 53 257 57 692.0 0 57 258 110 692.0 0 110 259 167 692.0 0 167 260 2443 692.0 0 2443 261 2760 692.0 0 2760 262 1810 692.0 0 1810 263 406 692.0 0 406 264 21 692.0 0 21 265 27 692.0 0 27 266 50 692.0 0 50 267 81 692.0 0 81 268 74 692.0 0 74 269 68 692.0 0 68 270 541 692.0 0 541 271 58 692.0 0 58 272 129 692.0 0 129 273 48 692.0 0 48 274 27 692.0 0 27 275 25 692.0 0 25 276 35 692.0 0 35 277 75 692.0 0 75 278 63 692.0 0 63 279 57 692.0 0 57 280 80 692.0 0 80 281 72 692.0 0 72 282 57 692.0 0 57 283 129 692.0 0 129 284 139 692.0 0 139 285 1055 692.0 0 1055 286 59 692.0 0 59 287 55 692.0 0 55 288 308 692.0 0 308 289 92 692.0 0 92 290 146 692.0 0 146 291 157 692.0 0 157 292 112 692.0 0 112 293 222 692.0 0 222 294 130 692.0 0 130 295 121 692.0 0 121 296 87 692.0 0 87 297 31 692.0 0 31 298 18 692.0 0 18 299 24 692.0 0 24 300 23 692.0 0 23 301 37 692.0 0 37 Finished in 6.28 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 138,767 Reads with adapters: 45,372 (32.7%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 45,356 (32.7%) Total basepairs processed: 36,693,434 bp Total written (filtered): 12,516,810 bp (34.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 45372 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 9 0.0 3 0 0 7 2 16 270 0.0 3 0 259 10 1 17 44810 0.0 3 44006 773 26 5 18 109 0.0 3 3 100 6 19 7 0.0 3 1 0 4 2 20 127 0.0 3 0 0 0 127 21 1 0.0 3 0 0 0 1 26 1 0.0 3 0 1 40 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 1 142 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 183 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 204 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 281 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.61 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 45,356 Reads with adapters: 141 (0.3%) Reads written (passing filters): 45,356 (100.0%) Total basepairs processed: 12,516,810 bp Total written (filtered): 12,516,291 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 141 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.2% C: 3.5% G: 68.8% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 45 708.7 0 45 4 96 177.2 0 96