This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.19 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 97,151 (3.4%) Reads that were too short: 13,481 (0.5%) Reads written (passing filters): 83,670 (3.0%) Total basepairs processed: 853,219,115 bp Total written (filtered): 22,399,028 bp (2.6%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 97151 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 756 692.0 0 756 7 34668 692.0 0 34668 8 26971 692.0 0 26971 9 57 692.0 0 57 10 27 692.0 0 27 11 26 692.0 0 26 12 31 692.0 0 31 13 24 692.0 0 24 14 27 692.0 0 27 15 29 692.0 0 29 16 29 692.0 0 29 17 28 692.0 0 28 18 186 692.0 0 186 19 38 692.0 0 38 20 80 692.0 0 80 21 26 692.0 0 26 22 31 692.0 0 31 23 41 692.0 0 41 24 30 692.0 0 30 25 33 692.0 0 33 26 47 692.0 0 47 27 63 692.0 0 63 28 43 692.0 0 43 29 17 692.0 0 17 30 41 692.0 0 41 31 27 692.0 0 27 32 38 692.0 0 38 33 39 692.0 0 39 34 37 692.0 0 37 35 47 692.0 0 47 36 30 692.0 0 30 37 41 692.0 0 41 38 37 692.0 0 37 39 47 692.0 0 47 40 43 692.0 0 43 41 32 692.0 0 32 42 30 692.0 0 30 43 36 692.0 0 36 44 49 692.0 0 49 45 34 692.0 0 34 46 40 692.0 0 40 47 35 692.0 0 35 48 59 692.0 0 59 49 40 692.0 0 40 50 350 692.0 0 350 51 25 692.0 0 25 52 49 692.0 0 49 53 40 692.0 0 40 54 58 692.0 0 58 55 34 692.0 0 34 56 37 692.0 0 37 57 28 692.0 0 28 58 52 692.0 0 52 59 52 692.0 0 52 60 30 692.0 0 30 61 49 692.0 0 49 62 42 692.0 0 42 63 35 692.0 0 35 64 30 692.0 0 30 65 64 692.0 0 64 66 81 692.0 0 81 67 83 692.0 0 83 68 77 692.0 0 77 69 127 692.0 0 127 70 143 692.0 0 143 71 1026 692.0 0 1026 72 3128 692.0 0 3128 73 777 692.0 0 777 74 54 692.0 0 54 75 45 692.0 0 45 76 39 692.0 0 39 77 80 692.0 0 80 78 44 692.0 0 44 79 72 692.0 0 72 80 73 692.0 0 73 81 67 692.0 0 67 82 60 692.0 0 60 83 49 692.0 0 49 84 74 692.0 0 74 85 101 692.0 0 101 86 62 692.0 0 62 87 94 692.0 0 94 88 37 692.0 0 37 89 47 692.0 0 47 90 67 692.0 0 67 91 64 692.0 0 64 92 59 692.0 0 59 93 77 692.0 0 77 94 121 692.0 0 121 95 147 692.0 0 147 96 1138 692.0 0 1138 97 939 692.0 0 939 98 264 692.0 0 264 99 81 692.0 0 81 100 27 692.0 0 27 101 29 692.0 0 29 102 38 692.0 0 38 103 53 692.0 0 53 104 65 692.0 0 65 105 45 692.0 0 45 106 124 692.0 0 124 107 43 692.0 0 43 108 53 692.0 0 53 109 110 692.0 0 110 110 47 692.0 0 47 111 40 692.0 0 40 112 61 692.0 0 61 113 53 692.0 0 53 114 58 692.0 0 58 115 55 692.0 0 55 116 53 692.0 0 53 117 53 692.0 0 53 118 68 692.0 0 68 119 46 692.0 0 46 120 39 692.0 0 39 121 35 692.0 0 35 122 42 692.0 0 42 123 49 692.0 0 49 124 154 692.0 0 154 125 99 692.0 0 99 126 193 692.0 0 193 127 109 692.0 0 109 128 152 692.0 0 152 129 102 692.0 0 102 130 117 692.0 0 117 131 87 692.0 0 87 132 115 692.0 0 115 133 78 692.0 0 78 134 75 692.0 0 75 135 115 692.0 0 115 136 66 692.0 0 66 137 67 692.0 0 67 138 63 692.0 0 63 139 35 692.0 0 35 140 67 692.0 0 67 141 60 692.0 0 60 142 57 692.0 0 57 143 74 692.0 0 74 144 153 692.0 0 153 145 121 692.0 0 121 146 77 692.0 0 77 147 49 692.0 0 49 148 44 692.0 0 44 149 46 692.0 0 46 150 62 692.0 0 62 151 56 692.0 0 56 152 90 692.0 0 90 153 51 692.0 0 51 154 73 692.0 0 73 155 108 692.0 0 108 156 60 692.0 0 60 157 37 692.0 0 37 158 47 692.0 0 47 159 52 692.0 0 52 160 48 692.0 0 48 161 34 692.0 0 34 162 63 692.0 0 63 163 44 692.0 0 44 164 61 692.0 0 61 165 98 692.0 0 98 166 64 692.0 0 64 167 58 692.0 0 58 168 43 692.0 0 43 169 55 692.0 0 55 170 51 692.0 0 51 171 61 692.0 0 61 172 118 692.0 0 118 173 352 692.0 0 352 174 184 692.0 0 184 175 255 692.0 0 255 176 82 692.0 0 82 177 73 692.0 0 73 178 49 692.0 0 49 179 58 692.0 0 58 180 69 692.0 0 69 181 55 692.0 0 55 182 51 692.0 0 51 183 66 692.0 0 66 184 46 692.0 0 46 185 95 692.0 0 95 186 95 692.0 0 95 187 74 692.0 0 74 188 62 692.0 0 62 189 54 692.0 0 54 190 98 692.0 0 98 191 121 692.0 0 121 192 608 692.0 0 608 193 596 692.0 0 596 194 398 692.0 0 398 195 142 692.0 0 142 196 57 692.0 0 57 197 46 692.0 0 46 198 61 692.0 0 61 199 80 692.0 0 80 200 117 692.0 0 117 201 69 692.0 0 69 202 58 692.0 0 58 203 65 692.0 0 65 204 621 692.0 0 621 205 70 692.0 0 70 206 122 692.0 0 122 207 108 692.0 0 108 208 73 692.0 0 73 209 149 692.0 0 149 210 133 692.0 0 133 211 216 692.0 0 216 212 139 692.0 0 139 213 59 692.0 0 59 214 58 692.0 0 58 215 149 692.0 0 149 216 58 692.0 0 58 217 102 692.0 0 102 218 95 692.0 0 95 219 106 692.0 0 106 220 137 692.0 0 137 221 158 692.0 0 158 222 78 692.0 0 78 223 49 692.0 0 49 224 52 692.0 0 52 225 79 692.0 0 79 226 135 692.0 0 135 227 105 692.0 0 105 228 130 692.0 0 130 229 67 692.0 0 67 230 62 692.0 0 62 231 74 692.0 0 74 232 58 692.0 0 58 233 69 692.0 0 69 234 74 692.0 0 74 235 76 692.0 0 76 236 95 692.0 0 95 237 99 692.0 0 99 238 98 692.0 0 98 239 60 692.0 0 60 240 98 692.0 0 98 241 104 692.0 0 104 242 120 692.0 0 120 243 120 692.0 0 120 244 59 692.0 0 59 245 75 692.0 0 75 246 89 692.0 0 89 247 170 692.0 0 170 248 75 692.0 0 75 249 146 692.0 0 146 250 80 692.0 0 80 251 77 692.0 0 77 252 57 692.0 0 57 253 65 692.0 0 65 254 112 692.0 0 112 255 151 692.0 0 151 256 118 692.0 0 118 257 111 692.0 0 111 258 131 692.0 0 131 259 124 692.0 0 124 260 105 692.0 0 105 261 86 692.0 0 86 262 97 692.0 0 97 263 79 692.0 0 79 264 67 692.0 0 67 265 74 692.0 0 74 266 138 692.0 0 138 267 208 692.0 0 208 268 178 692.0 0 178 269 108 692.0 0 108 270 175 692.0 0 175 271 146 692.0 0 146 272 564 692.0 0 564 273 293 692.0 0 293 274 802 692.0 0 802 275 138 692.0 0 138 276 118 692.0 0 118 277 515 692.0 0 515 278 103 692.0 0 103 279 119 692.0 0 119 280 130 692.0 0 130 281 136 692.0 0 136 282 89 692.0 0 89 283 70 692.0 0 70 284 77 692.0 0 77 285 112 692.0 0 112 286 169 692.0 0 169 287 125 692.0 0 125 288 179 692.0 0 179 289 116 692.0 0 116 290 126 692.0 0 126 291 117 692.0 0 117 292 116 692.0 0 116 293 145 692.0 0 145 294 319 692.0 0 319 295 235 692.0 0 235 296 411 692.0 0 411 297 109 692.0 0 109 298 72 692.0 0 72 299 92 692.0 0 92 300 134 692.0 0 134 301 71 692.0 0 71 Finished in 4.46 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 83,670 Reads with adapters: 28,671 (34.3%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 28,629 (34.2%) Total basepairs processed: 22,399,028 bp Total written (filtered): 7,761,942 bp (34.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28671 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 20 0.0 3 0 0 15 2 3 19 601 0.0 3 0 509 51 28 13 20 27312 0.0 4 25533 1472 222 67 18 21 55 0.0 4 8 41 2 2 2 22 7 0.0 4 2 0 1 1 3 23 109 0.0 4 0 0 0 73 36 24 2 0.0 4 0 0 0 0 2 27 1 0.0 4 1 29 1 0.0 4 0 0 1 43 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 1 73 1 0.0 4 0 0 0 0 1 80 1 0.0 4 1 84 1 0.0 4 0 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 1 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 1 122 1 0.0 4 1 126 5 0.0 4 0 0 0 0 5 127 491 0.0 4 0 0 0 0 491 130 3 0.0 4 0 0 0 0 3 131 1 0.0 4 0 1 153 6 0.0 4 0 0 0 0 6 168 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 216 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 1 228 14 0.0 4 0 0 0 1 13 229 5 0.0 4 0 0 0 0 5 230 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 1 3 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 28,629 Reads with adapters: 4,064 (14.2%) Reads written (passing filters): 28,629 (100.0%) Total basepairs processed: 7,761,942 bp Total written (filtered): 7,707,382 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4064 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 11.3% G: 82.0% T: 3.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 77 447.3 0 77 4 7 111.8 0 7 5 1 28.0 0 1 9 61 0.1 0 8 53 10 836 0.0 1 92 744 11 2908 0.0 1 132 2776 12 100 0.0 1 4 96 13 6 0.0 1 0 6 14 5 0.0 1 0 5 15 2 0.0 1 0 2 151 16 0.0 1 12 4 152 3 0.0 1 2 1 153 1 0.0 1 0 1 209 12 0.0 1 7 5 210 1 0.0 1 1 224 26 0.0 1 23 3 225 1 0.0 1 1 229 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.