This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.45 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 90,237 (3.2%) Reads that were too short: 7,896 (0.3%) Reads written (passing filters): 82,341 (2.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 22,431,024 bp (2.6%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 90237 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 627 692.0 0 627 7 31397 692.0 0 31397 8 32024 692.0 0 32024 9 43 692.0 0 43 10 31 692.0 0 31 11 20 692.0 0 20 12 19 692.0 0 19 13 28 692.0 0 28 14 25 692.0 0 25 15 25 692.0 0 25 16 14 692.0 0 14 17 25 692.0 0 25 18 51 692.0 0 51 19 29 692.0 0 29 20 53 692.0 0 53 21 19 692.0 0 19 22 28 692.0 0 28 23 22 692.0 0 22 24 20 692.0 0 20 25 21 692.0 0 21 26 26 692.0 0 26 27 21 692.0 0 21 28 20 692.0 0 20 29 21 692.0 0 21 30 41 692.0 0 41 31 31 692.0 0 31 32 28 692.0 0 28 33 42 692.0 0 42 34 47 692.0 0 47 35 45 692.0 0 45 36 47 692.0 0 47 37 49 692.0 0 49 38 36 692.0 0 36 39 49 692.0 0 49 40 24 692.0 0 24 41 40 692.0 0 40 42 37 692.0 0 37 43 29 692.0 0 29 44 55 692.0 0 55 45 29 692.0 0 29 46 38 692.0 0 38 47 39 692.0 0 39 48 75 692.0 0 75 49 42 692.0 0 42 50 360 692.0 0 360 51 43 692.0 0 43 52 53 692.0 0 53 53 28 692.0 0 28 54 45 692.0 0 45 55 36 692.0 0 36 56 43 692.0 0 43 57 30 692.0 0 30 58 39 692.0 0 39 59 56 692.0 0 56 60 35 692.0 0 35 61 44 692.0 0 44 62 34 692.0 0 34 63 28 692.0 0 28 64 36 692.0 0 36 65 47 692.0 0 47 66 57 692.0 0 57 67 56 692.0 0 56 68 63 692.0 0 63 69 142 692.0 0 142 70 164 692.0 0 164 71 1095 692.0 0 1095 72 3510 692.0 0 3510 73 906 692.0 0 906 74 34 692.0 0 34 75 34 692.0 0 34 76 22 692.0 0 22 77 63 692.0 0 63 78 33 692.0 0 33 79 67 692.0 0 67 80 46 692.0 0 46 81 39 692.0 0 39 82 29 692.0 0 29 83 26 692.0 0 26 84 24 692.0 0 24 85 44 692.0 0 44 86 49 692.0 0 49 87 57 692.0 0 57 88 37 692.0 0 37 89 64 692.0 0 64 90 37 692.0 0 37 91 51 692.0 0 51 92 42 692.0 0 42 93 66 692.0 0 66 94 85 692.0 0 85 95 101 692.0 0 101 96 1070 692.0 0 1070 97 1121 692.0 0 1121 98 243 692.0 0 243 99 81 692.0 0 81 100 32 692.0 0 32 101 34 692.0 0 34 102 38 692.0 0 38 103 37 692.0 0 37 104 56 692.0 0 56 105 37 692.0 0 37 106 89 692.0 0 89 107 40 692.0 0 40 108 46 692.0 0 46 109 89 692.0 0 89 110 26 692.0 0 26 111 29 692.0 0 29 112 27 692.0 0 27 113 29 692.0 0 29 114 45 692.0 0 45 115 59 692.0 0 59 116 44 692.0 0 44 117 29 692.0 0 29 118 56 692.0 0 56 119 36 692.0 0 36 120 25 692.0 0 25 121 26 692.0 0 26 122 36 692.0 0 36 123 52 692.0 0 52 124 59 692.0 0 59 125 66 692.0 0 66 126 87 692.0 0 87 127 73 692.0 0 73 128 56 692.0 0 56 129 47 692.0 0 47 130 73 692.0 0 73 131 54 692.0 0 54 132 104 692.0 0 104 133 53 692.0 0 53 134 62 692.0 0 62 135 71 692.0 0 71 136 42 692.0 0 42 137 58 692.0 0 58 138 46 692.0 0 46 139 36 692.0 0 36 140 45 692.0 0 45 141 45 692.0 0 45 142 54 692.0 0 54 143 73 692.0 0 73 144 99 692.0 0 99 145 83 692.0 0 83 146 44 692.0 0 44 147 40 692.0 0 40 148 32 692.0 0 32 149 32 692.0 0 32 150 33 692.0 0 33 151 40 692.0 0 40 152 105 692.0 0 105 153 39 692.0 0 39 154 49 692.0 0 49 155 45 692.0 0 45 156 32 692.0 0 32 157 56 692.0 0 56 158 42 692.0 0 42 159 23 692.0 0 23 160 68 692.0 0 68 161 38 692.0 0 38 162 40 692.0 0 40 163 40 692.0 0 40 164 43 692.0 0 43 165 52 692.0 0 52 166 50 692.0 0 50 167 42 692.0 0 42 168 38 692.0 0 38 169 36 692.0 0 36 170 36 692.0 0 36 171 49 692.0 0 49 172 66 692.0 0 66 173 193 692.0 0 193 174 100 692.0 0 100 175 155 692.0 0 155 176 37 692.0 0 37 177 50 692.0 0 50 178 26 692.0 0 26 179 49 692.0 0 49 180 40 692.0 0 40 181 41 692.0 0 41 182 42 692.0 0 42 183 43 692.0 0 43 184 40 692.0 0 40 185 49 692.0 0 49 186 44 692.0 0 44 187 42 692.0 0 42 188 48 692.0 0 48 189 56 692.0 0 56 190 57 692.0 0 57 191 85 692.0 0 85 192 486 692.0 0 486 193 486 692.0 0 486 194 254 692.0 0 254 195 105 692.0 0 105 196 51 692.0 0 51 197 42 692.0 0 42 198 28 692.0 0 28 199 40 692.0 0 40 200 48 692.0 0 48 201 44 692.0 0 44 202 43 692.0 0 43 203 53 692.0 0 53 204 639 692.0 0 639 205 48 692.0 0 48 206 82 692.0 0 82 207 67 692.0 0 67 208 35 692.0 0 35 209 67 692.0 0 67 210 47 692.0 0 47 211 74 692.0 0 74 212 53 692.0 0 53 213 49 692.0 0 49 214 29 692.0 0 29 215 115 692.0 0 115 216 32 692.0 0 32 217 55 692.0 0 55 218 58 692.0 0 58 219 47 692.0 0 47 220 59 692.0 0 59 221 49 692.0 0 49 222 34 692.0 0 34 223 38 692.0 0 38 224 32 692.0 0 32 225 84 692.0 0 84 226 98 692.0 0 98 227 58 692.0 0 58 228 39 692.0 0 39 229 52 692.0 0 52 230 48 692.0 0 48 231 46 692.0 0 46 232 31 692.0 0 31 233 43 692.0 0 43 234 42 692.0 0 42 235 54 692.0 0 54 236 48 692.0 0 48 237 57 692.0 0 57 238 60 692.0 0 60 239 32 692.0 0 32 240 41 692.0 0 41 241 37 692.0 0 37 242 41 692.0 0 41 243 45 692.0 0 45 244 24 692.0 0 24 245 28 692.0 0 28 246 39 692.0 0 39 247 89 692.0 0 89 248 42 692.0 0 42 249 75 692.0 0 75 250 52 692.0 0 52 251 32 692.0 0 32 252 45 692.0 0 45 253 53 692.0 0 53 254 77 692.0 0 77 255 53 692.0 0 53 256 58 692.0 0 58 257 61 692.0 0 61 258 61 692.0 0 61 259 67 692.0 0 67 260 55 692.0 0 55 261 49 692.0 0 49 262 46 692.0 0 46 263 54 692.0 0 54 264 34 692.0 0 34 265 61 692.0 0 61 266 117 692.0 0 117 267 99 692.0 0 99 268 70 692.0 0 70 269 71 692.0 0 71 270 95 692.0 0 95 271 93 692.0 0 93 272 546 692.0 0 546 273 133 692.0 0 133 274 322 692.0 0 322 275 68 692.0 0 68 276 56 692.0 0 56 277 644 692.0 0 644 278 83 692.0 0 83 279 53 692.0 0 53 280 67 692.0 0 67 281 57 692.0 0 57 282 46 692.0 0 46 283 43 692.0 0 43 284 42 692.0 0 42 285 57 692.0 0 57 286 72 692.0 0 72 287 73 692.0 0 73 288 104 692.0 0 104 289 52 692.0 0 52 290 88 692.0 0 88 291 67 692.0 0 67 292 56 692.0 0 56 293 62 692.0 0 62 294 104 692.0 0 104 295 95 692.0 0 95 296 129 692.0 0 129 297 48 692.0 0 48 298 52 692.0 0 52 299 46 692.0 0 46 300 80 692.0 0 80 301 90 692.0 0 90 Finished in 3.98 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 82,341 Reads with adapters: 30,742 (37.3%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 30,708 (37.3%) Total basepairs processed: 22,431,024 bp Total written (filtered): 8,499,348 bp (37.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30742 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 186 0.0 3 0 184 2 17 29061 0.0 3 28490 551 18 2 18 56 0.0 3 3 51 2 19 1304 0.0 3 1262 28 12 2 20 66 0.0 3 0 3 0 63 21 1 0.0 3 1 22 3 0.0 3 1 0 0 2 23 1 0.0 3 1 24 1 0.0 3 1 32 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 1 86 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 116 3 0.0 3 0 0 0 3 118 1 0.0 3 1 121 3 0.0 3 3 126 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 208 2 0.0 3 1 0 0 1 228 6 0.0 3 0 0 0 6 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 30,708 Reads with adapters: 72 (0.2%) Reads written (passing filters): 30,708 (100.0%) Total basepairs processed: 8,499,348 bp Total written (filtered): 8,499,119 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 72 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 23.6% G: 22.2% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 64 479.8 0 64 4 3 120.0 0 3 5 5 30.0 0 5