This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.97 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 218,026 (7.7%) Reads that were too short: 6,078 (0.2%) Reads written (passing filters): 211,948 (7.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 59,545,281 bp (7.0%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 218026 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1919 692.0 0 1919 7 83096 692.0 0 83096 8 3093 692.0 0 3093 9 96203 692.0 0 96203 10 71 692.0 0 71 11 90 692.0 0 90 12 49 692.0 0 49 13 84 692.0 0 84 14 40 692.0 0 40 15 36 692.0 0 36 16 107 692.0 0 107 17 80 692.0 0 80 18 220 692.0 0 220 19 38 692.0 0 38 20 35 692.0 0 35 21 27 692.0 0 27 22 43 692.0 0 43 23 34 692.0 0 34 24 34 692.0 0 34 25 30 692.0 0 30 26 30 692.0 0 30 27 22 692.0 0 22 28 21 692.0 0 21 29 30 692.0 0 30 30 33 692.0 0 33 31 45 692.0 0 45 32 49 692.0 0 49 33 84 692.0 0 84 34 33 692.0 0 33 35 30 692.0 0 30 36 31 692.0 0 31 37 48 692.0 0 48 38 49 692.0 0 49 39 38 692.0 0 38 40 31 692.0 0 31 41 34 692.0 0 34 42 48 692.0 0 48 43 42 692.0 0 42 44 42 692.0 0 42 45 54 692.0 0 54 46 289 692.0 0 289 47 331 692.0 0 331 48 302 692.0 0 302 49 52 692.0 0 52 50 39 692.0 0 39 51 44 692.0 0 44 52 59 692.0 0 59 53 37 692.0 0 37 54 36 692.0 0 36 55 43 692.0 0 43 56 46 692.0 0 46 57 31 692.0 0 31 58 42 692.0 0 42 59 61 692.0 0 61 60 63 692.0 0 63 61 47 692.0 0 47 62 49 692.0 0 49 63 74 692.0 0 74 64 47 692.0 0 47 65 60 692.0 0 60 66 53 692.0 0 53 67 55 692.0 0 55 68 51 692.0 0 51 69 43 692.0 0 43 70 55 692.0 0 55 71 39 692.0 0 39 72 52 692.0 0 52 73 58 692.0 0 58 74 55 692.0 0 55 75 42 692.0 0 42 76 71 692.0 0 71 77 62 692.0 0 62 78 84 692.0 0 84 79 244 692.0 0 244 80 388 692.0 0 388 81 755 692.0 0 755 82 150 692.0 0 150 83 172 692.0 0 172 84 187 692.0 0 187 85 121 692.0 0 121 86 140 692.0 0 140 87 115 692.0 0 115 88 100 692.0 0 100 89 118 692.0 0 118 90 98 692.0 0 98 91 119 692.0 0 119 92 169 692.0 0 169 93 188 692.0 0 188 94 2716 692.0 0 2716 95 4571 692.0 0 4571 96 4647 692.0 0 4647 97 59 692.0 0 59 98 189 692.0 0 189 99 37 692.0 0 37 100 37 692.0 0 37 101 43 692.0 0 43 102 31 692.0 0 31 103 41 692.0 0 41 104 37 692.0 0 37 105 33 692.0 0 33 106 27 692.0 0 27 107 42 692.0 0 42 108 48 692.0 0 48 109 36 692.0 0 36 110 55 692.0 0 55 111 61 692.0 0 61 112 64 692.0 0 64 113 50 692.0 0 50 114 51 692.0 0 51 115 38 692.0 0 38 116 49 692.0 0 49 117 61 692.0 0 61 118 115 692.0 0 115 119 56 692.0 0 56 120 81 692.0 0 81 121 50 692.0 0 50 122 38 692.0 0 38 123 59 692.0 0 59 124 257 692.0 0 257 125 575 692.0 0 575 126 313 692.0 0 313 127 78 692.0 0 78 128 41 692.0 0 41 129 57 692.0 0 57 130 93 692.0 0 93 131 142 692.0 0 142 132 112 692.0 0 112 133 340 692.0 0 340 134 75 692.0 0 75 135 61 692.0 0 61 136 118 692.0 0 118 137 312 692.0 0 312 138 389 692.0 0 389 139 410 692.0 0 410 140 86 692.0 0 86 141 134 692.0 0 134 142 63 692.0 0 63 143 87 692.0 0 87 144 186 692.0 0 186 145 101 692.0 0 101 146 159 692.0 0 159 147 86 692.0 0 86 148 74 692.0 0 74 149 141 692.0 0 141 150 43 692.0 0 43 151 38 692.0 0 38 152 36 692.0 0 36 153 44 692.0 0 44 154 38 692.0 0 38 155 32 692.0 0 32 156 50 692.0 0 50 157 58 692.0 0 58 158 123 692.0 0 123 159 65 692.0 0 65 160 78 692.0 0 78 161 42 692.0 0 42 162 24 692.0 0 24 163 51 692.0 0 51 164 40 692.0 0 40 165 42 692.0 0 42 166 48 692.0 0 48 167 39 692.0 0 39 168 42 692.0 0 42 169 32 692.0 0 32 170 49 692.0 0 49 171 24 692.0 0 24 172 37 692.0 0 37 173 43 692.0 0 43 174 30 692.0 0 30 175 45 692.0 0 45 176 33 692.0 0 33 177 80 692.0 0 80 178 60 692.0 0 60 179 38 692.0 0 38 180 51 692.0 0 51 181 35 692.0 0 35 182 50 692.0 0 50 183 35 692.0 0 35 184 41 692.0 0 41 185 35 692.0 0 35 186 43 692.0 0 43 187 44 692.0 0 44 188 63 692.0 0 63 189 53 692.0 0 53 190 42 692.0 0 42 191 36 692.0 0 36 192 61 692.0 0 61 193 84 692.0 0 84 194 73 692.0 0 73 195 35 692.0 0 35 196 35 692.0 0 35 197 38 692.0 0 38 198 31 692.0 0 31 199 29 692.0 0 29 200 30 692.0 0 30 201 29 692.0 0 29 202 37 692.0 0 37 203 50 692.0 0 50 204 64 692.0 0 64 205 208 692.0 0 208 206 104 692.0 0 104 207 216 692.0 0 216 208 39 692.0 0 39 209 48 692.0 0 48 210 43 692.0 0 43 211 41 692.0 0 41 212 45 692.0 0 45 213 47 692.0 0 47 214 20 692.0 0 20 215 26 692.0 0 26 216 29 692.0 0 29 217 37 692.0 0 37 218 33 692.0 0 33 219 21 692.0 0 21 220 29 692.0 0 29 221 35 692.0 0 35 222 37 692.0 0 37 223 39 692.0 0 39 224 33 692.0 0 33 225 32 692.0 0 32 226 30 692.0 0 30 227 41 692.0 0 41 228 47 692.0 0 47 229 61 692.0 0 61 230 36 692.0 0 36 231 31 692.0 0 31 232 59 692.0 0 59 233 58 692.0 0 58 234 51 692.0 0 51 235 24 692.0 0 24 236 30 692.0 0 30 237 44 692.0 0 44 238 54 692.0 0 54 239 41 692.0 0 41 240 49 692.0 0 49 241 50 692.0 0 50 242 39 692.0 0 39 243 32 692.0 0 32 244 33 692.0 0 33 245 53 692.0 0 53 246 29 692.0 0 29 247 37 692.0 0 37 248 31 692.0 0 31 249 39 692.0 0 39 250 42 692.0 0 42 251 40 692.0 0 40 252 31 692.0 0 31 253 47 692.0 0 47 254 54 692.0 0 54 255 50 692.0 0 50 256 61 692.0 0 61 257 47 692.0 0 47 258 40 692.0 0 40 259 47 692.0 0 47 260 41 692.0 0 41 261 74 692.0 0 74 262 79 692.0 0 79 263 111 692.0 0 111 264 47 692.0 0 47 265 43 692.0 0 43 266 47 692.0 0 47 267 72 692.0 0 72 268 43 692.0 0 43 269 56 692.0 0 56 270 86 692.0 0 86 271 99 692.0 0 99 272 153 692.0 0 153 273 61 692.0 0 61 274 62 692.0 0 62 275 62 692.0 0 62 276 83 692.0 0 83 277 68 692.0 0 68 278 53 692.0 0 53 279 73 692.0 0 73 280 42 692.0 0 42 281 90 692.0 0 90 282 95 692.0 0 95 283 108 692.0 0 108 284 189 692.0 0 189 285 178 692.0 0 178 286 125 692.0 0 125 287 81 692.0 0 81 288 121 692.0 0 121 289 67 692.0 0 67 290 90 692.0 0 90 291 63 692.0 0 63 292 55 692.0 0 55 293 41 692.0 0 41 294 77 692.0 0 77 295 58 692.0 0 58 296 60 692.0 0 60 297 66 692.0 0 66 298 83 692.0 0 83 299 79 692.0 0 79 300 91 692.0 0 91 301 35 692.0 0 35 Finished in 10.58 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 211,948 Reads with adapters: 82,963 (39.1%) Reads that were too short: 129 (0.1%) Reads written (passing filters): 82,834 (39.1%) Total basepairs processed: 59,545,281 bp Total written (filtered): 22,656,336 bp (38.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 82963 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 4 18 34 0.0 3 0 0 22 8 4 19 905 0.0 3 0 798 66 29 12 20 81114 0.0 4 77094 3331 525 128 36 21 230 0.0 4 20 194 11 2 3 22 21 0.0 4 2 0 14 3 2 23 142 0.0 4 2 0 1 112 27 24 5 0.0 4 2 0 0 0 3 28 3 0.0 4 0 0 0 0 3 32 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 1 77 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 115 3 0.0 4 0 0 0 0 3 116 3 0.0 4 0 0 0 1 2 126 6 0.0 4 0 0 0 0 6 127 351 0.0 4 0 0 0 0 351 128 4 0.0 4 0 0 0 0 4 130 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 52 0.0 4 0 0 0 1 51 142 15 0.0 4 0 0 0 1 14 143 2 0.0 4 0 0 0 0 2 153 9 0.0 4 0 0 0 1 8 159 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 1 185 1 0.0 4 0 0 0 0 1 189 4 0.0 4 0 0 0 0 4 191 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 224 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 5 0.0 4 0 0 0 0 5 235 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.86 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 82,834 Reads with adapters: 3,358 (4.1%) Reads written (passing filters): 82,834 (100.0%) Total basepairs processed: 22,656,336 bp Total written (filtered): 22,586,539 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3358 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.7% C: 14.7% G: 67.8% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 404 1294.3 0 404 4 34 323.6 0 34 5 4 80.9 0 4 8 2 1.3 0 2 9 17 0.3 0 3 14 10 67 0.1 1 10 57 11 1660 0.0 1 91 1569 12 923 0.0 1 31 892 13 37 0.0 1 3 34 15 11 0.0 1 0 11 16 1 0.0 1 0 1 24 4 0.0 1 3 1 35 1 0.0 1 1 36 1 0.0 1 1 108 1 0.0 1 0 1 133 1 0.0 1 1 149 1 0.0 1 1 152 42 0.0 1 26 16 153 1 0.0 1 1 158 1 0.0 1 1 191 5 0.0 1 0 5 192 1 0.0 1 0 1 193 2 0.0 1 0 2 210 133 0.0 1 108 25 211 2 0.0 1 2 225 1 0.0 1 1 230 1 0.0 1 1