This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.04 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 497,210 (17.5%) Reads that were too short: 21,956 (0.8%) Reads written (passing filters): 475,254 (16.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 89,115,646 bp (10.4%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 497210 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 531 692.0 0 531 7 11256 692.0 0 11256 8 452 692.0 0 452 9 7742 692.0 0 7742 10 37 692.0 0 37 11 36 692.0 0 36 12 23 692.0 0 23 13 29 692.0 0 29 14 122 692.0 0 122 15 65 692.0 0 65 16 69 692.0 0 69 17 22 692.0 0 22 18 14 692.0 0 14 19 26 692.0 0 26 20 13 692.0 0 13 21 36 692.0 0 36 22 42 692.0 0 42 23 159 692.0 0 159 24 184 692.0 0 184 25 44 692.0 0 44 26 17 692.0 0 17 27 40 692.0 0 40 28 33 692.0 0 33 29 35 692.0 0 35 30 33 692.0 0 33 31 48 692.0 0 48 32 40 692.0 0 40 33 94 692.0 0 94 34 79 692.0 0 79 35 155 692.0 0 155 36 67 692.0 0 67 37 61 692.0 0 61 38 58 692.0 0 58 39 59 692.0 0 59 40 93 692.0 0 93 41 57 692.0 0 57 42 48 692.0 0 48 43 49 692.0 0 49 44 38 692.0 0 38 45 44 692.0 0 44 46 92 692.0 0 92 47 39 692.0 0 39 48 68 692.0 0 68 49 47 692.0 0 47 50 61 692.0 0 61 51 66 692.0 0 66 52 87 692.0 0 87 53 100 692.0 0 100 54 49 692.0 0 49 55 41 692.0 0 41 56 82 692.0 0 82 57 153 692.0 0 153 58 241 692.0 0 241 59 336 692.0 0 336 60 85 692.0 0 85 61 157 692.0 0 157 62 56 692.0 0 56 63 268 692.0 0 268 64 57 692.0 0 57 65 60 692.0 0 60 66 49 692.0 0 49 67 76 692.0 0 76 68 32 692.0 0 32 69 60 692.0 0 60 70 57 692.0 0 57 71 80 692.0 0 80 72 77 692.0 0 77 73 40 692.0 0 40 74 71 692.0 0 71 75 56 692.0 0 56 76 87 692.0 0 87 77 755 692.0 0 755 78 226 692.0 0 226 79 85 692.0 0 85 80 130 692.0 0 130 81 82 692.0 0 82 82 66 692.0 0 66 83 112 692.0 0 112 84 180 692.0 0 180 85 91 692.0 0 91 86 217 692.0 0 217 87 618 692.0 0 618 88 509 692.0 0 509 89 170 692.0 0 170 90 133 692.0 0 133 91 163 692.0 0 163 92 128 692.0 0 128 93 86 692.0 0 86 94 166 692.0 0 166 95 101 692.0 0 101 96 295 692.0 0 295 97 877 692.0 0 877 98 248 692.0 0 248 99 565 692.0 0 565 100 459 692.0 0 459 101 249 692.0 0 249 102 556 692.0 0 556 103 186 692.0 0 186 104 163 692.0 0 163 105 181 692.0 0 181 106 668 692.0 0 668 107 712 692.0 0 712 108 1611 692.0 0 1611 109 1101 692.0 0 1101 110 1733 692.0 0 1733 111 3003 692.0 0 3003 112 7131 692.0 0 7131 113 20190 692.0 0 20190 114 213867 692.0 0 213867 115 49383 692.0 0 49383 116 85758 692.0 0 85758 117 434 692.0 0 434 118 161 692.0 0 161 119 60 692.0 0 60 120 57 692.0 0 57 121 41 692.0 0 41 122 44 692.0 0 44 123 54 692.0 0 54 124 71 692.0 0 71 125 67 692.0 0 67 126 172 692.0 0 172 127 135 692.0 0 135 128 225 692.0 0 225 129 215 692.0 0 215 130 121 692.0 0 121 131 104 692.0 0 104 132 66 692.0 0 66 133 59 692.0 0 59 134 63 692.0 0 63 135 95 692.0 0 95 136 139 692.0 0 139 137 93 692.0 0 93 138 146 692.0 0 146 139 218 692.0 0 218 140 785 692.0 0 785 141 719 692.0 0 719 142 1987 692.0 0 1987 143 482 692.0 0 482 144 439 692.0 0 439 145 301 692.0 0 301 146 373 692.0 0 373 147 4444 692.0 0 4444 148 6406 692.0 0 6406 149 3068 692.0 0 3068 150 422 692.0 0 422 151 516 692.0 0 516 152 901 692.0 0 901 153 16099 692.0 0 16099 154 5216 692.0 0 5216 155 4175 692.0 0 4175 156 893 692.0 0 893 157 390 692.0 0 390 158 304 692.0 0 304 159 44 692.0 0 44 160 122 692.0 0 122 161 95 692.0 0 95 162 154 692.0 0 154 163 124 692.0 0 124 164 103 692.0 0 103 165 111 692.0 0 111 166 58 692.0 0 58 167 123 692.0 0 123 168 398 692.0 0 398 169 358 692.0 0 358 170 647 692.0 0 647 171 111 692.0 0 111 172 47 692.0 0 47 173 29 692.0 0 29 174 25 692.0 0 25 175 40 692.0 0 40 176 130 692.0 0 130 177 54 692.0 0 54 178 65 692.0 0 65 179 51 692.0 0 51 180 59 692.0 0 59 181 164 692.0 0 164 182 109 692.0 0 109 183 188 692.0 0 188 184 549 692.0 0 549 185 382 692.0 0 382 186 877 692.0 0 877 187 258 692.0 0 258 188 137 692.0 0 137 189 57 692.0 0 57 190 61 692.0 0 61 191 81 692.0 0 81 192 89 692.0 0 89 193 60 692.0 0 60 194 55 692.0 0 55 195 34 692.0 0 34 196 58 692.0 0 58 197 81 692.0 0 81 198 88 692.0 0 88 199 273 692.0 0 273 200 60 692.0 0 60 201 51 692.0 0 51 202 168 692.0 0 168 203 193 692.0 0 193 204 129 692.0 0 129 205 75 692.0 0 75 206 51 692.0 0 51 207 45 692.0 0 45 208 35 692.0 0 35 209 37 692.0 0 37 210 34 692.0 0 34 211 49 692.0 0 49 212 31 692.0 0 31 213 57 692.0 0 57 214 61 692.0 0 61 215 68 692.0 0 68 216 682 692.0 0 682 217 218 692.0 0 218 218 240 692.0 0 240 219 57 692.0 0 57 220 36 692.0 0 36 221 42 692.0 0 42 222 47 692.0 0 47 223 44 692.0 0 44 224 39 692.0 0 39 225 47 692.0 0 47 226 98 692.0 0 98 227 66 692.0 0 66 228 77 692.0 0 77 229 47 692.0 0 47 230 51 692.0 0 51 231 55 692.0 0 55 232 75 692.0 0 75 233 125 692.0 0 125 234 183 692.0 0 183 235 357 692.0 0 357 236 143 692.0 0 143 237 184 692.0 0 184 238 93 692.0 0 93 239 79 692.0 0 79 240 39 692.0 0 39 241 51 692.0 0 51 242 51 692.0 0 51 243 51 692.0 0 51 244 102 692.0 0 102 245 77 692.0 0 77 246 171 692.0 0 171 247 91 692.0 0 91 248 90 692.0 0 90 249 284 692.0 0 284 250 117 692.0 0 117 251 236 692.0 0 236 252 83 692.0 0 83 253 93 692.0 0 93 254 99 692.0 0 99 255 104 692.0 0 104 256 98 692.0 0 98 257 96 692.0 0 96 258 62 692.0 0 62 259 66 692.0 0 66 260 71 692.0 0 71 261 85 692.0 0 85 262 78 692.0 0 78 263 139 692.0 0 139 264 102 692.0 0 102 265 97 692.0 0 97 266 63 692.0 0 63 267 114 692.0 0 114 268 127 692.0 0 127 269 199 692.0 0 199 270 236 692.0 0 236 271 533 692.0 0 533 272 175 692.0 0 175 273 91 692.0 0 91 274 204 692.0 0 204 275 172 692.0 0 172 276 226 692.0 0 226 277 153 692.0 0 153 278 167 692.0 0 167 279 737 692.0 0 737 280 1311 692.0 0 1311 281 2805 692.0 0 2805 282 1169 692.0 0 1169 283 2162 692.0 0 2162 284 180 692.0 0 180 285 308 692.0 0 308 286 143 692.0 0 143 287 204 692.0 0 204 288 86 692.0 0 86 289 176 692.0 0 176 290 440 692.0 0 440 291 348 692.0 0 348 292 442 692.0 0 442 293 244 692.0 0 244 294 259 692.0 0 259 295 314 692.0 0 314 296 414 692.0 0 414 297 392 692.0 0 392 298 377 692.0 0 377 299 132 692.0 0 132 300 48 692.0 0 48 301 54 692.0 0 54 Finished in 17.58 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 475,254 Reads with adapters: 8,397 (1.8%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 8,354 (1.8%) Total basepairs processed: 89,115,646 bp Total written (filtered): 2,268,114 bp (2.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8397 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 10 0.0 3 0 0 8 2 19 138 0.0 3 0 114 13 6 5 20 8070 0.0 4 7487 464 83 26 10 21 32 0.0 4 10 15 4 0 3 22 7 0.0 4 0 0 0 2 5 23 54 0.0 4 0 0 1 43 10 24 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 1 48 2 0.0 4 1 0 0 0 1 51 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 80 2 0.0 4 1 0 0 0 1 82 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 1 97 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 109 1 0.0 4 0 0 0 1 110 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 25 0.0 4 0 0 0 0 25 131 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 1 136 2 0.0 4 1 0 0 0 1 140 2 0.0 4 0 1 0 1 141 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 158 2 0.0 4 0 1 0 1 162 1 0.0 4 0 0 1 163 1 0.0 4 0 0 0 0 1 165 1 0.0 4 1 171 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 188 1 0.0 4 1 190 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 1 213 1 0.0 4 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 8,354 Reads with adapters: 2,062 (24.7%) Reads written (passing filters): 8,354 (100.0%) Total basepairs processed: 2,268,114 bp Total written (filtered): 2,240,901 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2062 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 7.2% G: 86.2% T: 2.5% none/other: 0.2% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 130.5 0 4 4 1 32.6 0 1 7 1 0.5 0 1 9 126 0.0 0 14 112 10 1611 0.0 1 144 1467 11 273 0.0 1 12 261 12 4 0.0 1 0 4 13 8 0.0 1 0 8 18 1 0.0 1 1 19 1 0.0 1 1 138 1 0.0 1 1 150 2 0.0 1 1 1 169 1 0.0 1 1 208 21 0.0 1 13 8 209 1 0.0 1 1 211 1 0.0 1 1 271 1 0.0 1 1 272 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.