This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.35 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 665,818 (23.5%) Reads that were too short: 11,029 (0.4%) Reads written (passing filters): 654,789 (23.1%) Total basepairs processed: 853,219,115 bp Total written (filtered): 122,693,888 bp (14.4%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 665818 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 361 692.0 0 361 7 10082 692.0 0 10082 8 417 692.0 0 417 9 9643 692.0 0 9643 10 20 692.0 0 20 11 13 692.0 0 13 12 27 692.0 0 27 13 25 692.0 0 25 14 23 692.0 0 23 15 50 692.0 0 50 16 27 692.0 0 27 17 24 692.0 0 24 18 11 692.0 0 11 19 22 692.0 0 22 20 32 692.0 0 32 21 30 692.0 0 30 22 27 692.0 0 27 23 32 692.0 0 32 24 32 692.0 0 32 25 47 692.0 0 47 26 35 692.0 0 35 27 29 692.0 0 29 28 28 692.0 0 28 29 21 692.0 0 21 30 34 692.0 0 34 31 44 692.0 0 44 32 34 692.0 0 34 33 51 692.0 0 51 34 34 692.0 0 34 35 36 692.0 0 36 36 61 692.0 0 61 37 43 692.0 0 43 38 37 692.0 0 37 39 45 692.0 0 45 40 47 692.0 0 47 41 37 692.0 0 37 42 40 692.0 0 40 43 40 692.0 0 40 44 27 692.0 0 27 45 49 692.0 0 49 46 37 692.0 0 37 47 40 692.0 0 40 48 43 692.0 0 43 49 32 692.0 0 32 50 44 692.0 0 44 51 75 692.0 0 75 52 81 692.0 0 81 53 100 692.0 0 100 54 49 692.0 0 49 55 48 692.0 0 48 56 51 692.0 0 51 57 81 692.0 0 81 58 99 692.0 0 99 59 1886 692.0 0 1886 60 244 692.0 0 244 61 101 692.0 0 101 62 84 692.0 0 84 63 282 692.0 0 282 64 54 692.0 0 54 65 35 692.0 0 35 66 53 692.0 0 53 67 83 692.0 0 83 68 47 692.0 0 47 69 40 692.0 0 40 70 51 692.0 0 51 71 92 692.0 0 92 72 46 692.0 0 46 73 62 692.0 0 62 74 67 692.0 0 67 75 59 692.0 0 59 76 126 692.0 0 126 77 1011 692.0 0 1011 78 279 692.0 0 279 79 77 692.0 0 77 80 120 692.0 0 120 81 84 692.0 0 84 82 60 692.0 0 60 83 116 692.0 0 116 84 203 692.0 0 203 85 85 692.0 0 85 86 215 692.0 0 215 87 796 692.0 0 796 88 526 692.0 0 526 89 82 692.0 0 82 90 81 692.0 0 81 91 80 692.0 0 80 92 95 692.0 0 95 93 77 692.0 0 77 94 169 692.0 0 169 95 121 692.0 0 121 96 417 692.0 0 417 97 1248 692.0 0 1248 98 341 692.0 0 341 99 860 692.0 0 860 100 623 692.0 0 623 101 293 692.0 0 293 102 775 692.0 0 775 103 243 692.0 0 243 104 167 692.0 0 167 105 152 692.0 0 152 106 443 692.0 0 443 107 751 692.0 0 751 108 991 692.0 0 991 109 1352 692.0 0 1352 110 2226 692.0 0 2226 111 3939 692.0 0 3939 112 10140 692.0 0 10140 113 28591 692.0 0 28591 114 316630 692.0 0 316630 115 70466 692.0 0 70466 116 125367 692.0 0 125367 117 493 692.0 0 493 118 140 692.0 0 140 119 50 692.0 0 50 120 59 692.0 0 59 121 41 692.0 0 41 122 49 692.0 0 49 123 54 692.0 0 54 124 75 692.0 0 75 125 69 692.0 0 69 126 142 692.0 0 142 127 166 692.0 0 166 128 204 692.0 0 204 129 168 692.0 0 168 130 93 692.0 0 93 131 68 692.0 0 68 132 61 692.0 0 61 133 47 692.0 0 47 134 53 692.0 0 53 135 118 692.0 0 118 136 130 692.0 0 130 137 69 692.0 0 69 138 119 692.0 0 119 139 190 692.0 0 190 140 244 692.0 0 244 141 401 692.0 0 401 142 1052 692.0 0 1052 143 500 692.0 0 500 144 453 692.0 0 453 145 323 692.0 0 323 146 332 692.0 0 332 147 5230 692.0 0 5230 148 7805 692.0 0 7805 149 3739 692.0 0 3739 150 404 692.0 0 404 151 521 692.0 0 521 152 937 692.0 0 937 153 18938 692.0 0 18938 154 5972 692.0 0 5972 155 4310 692.0 0 4310 156 368 692.0 0 368 157 235 692.0 0 235 158 114 692.0 0 114 159 29 692.0 0 29 160 71 692.0 0 71 161 41 692.0 0 41 162 59 692.0 0 59 163 101 692.0 0 101 164 60 692.0 0 60 165 40 692.0 0 40 166 26 692.0 0 26 167 62 692.0 0 62 168 135 692.0 0 135 169 106 692.0 0 106 170 374 692.0 0 374 171 67 692.0 0 67 172 34 692.0 0 34 173 30 692.0 0 30 174 31 692.0 0 31 175 35 692.0 0 35 176 73 692.0 0 73 177 43 692.0 0 43 178 44 692.0 0 44 179 25 692.0 0 25 180 39 692.0 0 39 181 150 692.0 0 150 182 97 692.0 0 97 183 136 692.0 0 136 184 408 692.0 0 408 185 159 692.0 0 159 186 691 692.0 0 691 187 75 692.0 0 75 188 55 692.0 0 55 189 35 692.0 0 35 190 33 692.0 0 33 191 35 692.0 0 35 192 42 692.0 0 42 193 34 692.0 0 34 194 30 692.0 0 30 195 30 692.0 0 30 196 34 692.0 0 34 197 51 692.0 0 51 198 50 692.0 0 50 199 280 692.0 0 280 200 44 692.0 0 44 201 58 692.0 0 58 202 161 692.0 0 161 203 238 692.0 0 238 204 99 692.0 0 99 205 43 692.0 0 43 206 23 692.0 0 23 207 31 692.0 0 31 208 33 692.0 0 33 209 27 692.0 0 27 210 28 692.0 0 28 211 42 692.0 0 42 212 39 692.0 0 39 213 43 692.0 0 43 214 44 692.0 0 44 215 66 692.0 0 66 216 449 692.0 0 449 217 102 692.0 0 102 218 109 692.0 0 109 219 41 692.0 0 41 220 19 692.0 0 19 221 27 692.0 0 27 222 76 692.0 0 76 223 25 692.0 0 25 224 33 692.0 0 33 225 33 692.0 0 33 226 82 692.0 0 82 227 30 692.0 0 30 228 44 692.0 0 44 229 33 692.0 0 33 230 34 692.0 0 34 231 43 692.0 0 43 232 44 692.0 0 44 233 60 692.0 0 60 234 77 692.0 0 77 235 112 692.0 0 112 236 82 692.0 0 82 237 111 692.0 0 111 238 54 692.0 0 54 239 56 692.0 0 56 240 37 692.0 0 37 241 50 692.0 0 50 242 31 692.0 0 31 243 42 692.0 0 42 244 42 692.0 0 42 245 35 692.0 0 35 246 57 692.0 0 57 247 77 692.0 0 77 248 32 692.0 0 32 249 74 692.0 0 74 250 49 692.0 0 49 251 92 692.0 0 92 252 48 692.0 0 48 253 53 692.0 0 53 254 50 692.0 0 50 255 34 692.0 0 34 256 38 692.0 0 38 257 32 692.0 0 32 258 41 692.0 0 41 259 47 692.0 0 47 260 51 692.0 0 51 261 55 692.0 0 55 262 52 692.0 0 52 263 83 692.0 0 83 264 69 692.0 0 69 265 67 692.0 0 67 266 57 692.0 0 57 267 54 692.0 0 54 268 66 692.0 0 66 269 79 692.0 0 79 270 93 692.0 0 93 271 166 692.0 0 166 272 87 692.0 0 87 273 63 692.0 0 63 274 134 692.0 0 134 275 101 692.0 0 101 276 83 692.0 0 83 277 78 692.0 0 78 278 106 692.0 0 106 279 279 692.0 0 279 280 515 692.0 0 515 281 1249 692.0 0 1249 282 551 692.0 0 551 283 1027 692.0 0 1027 284 121 692.0 0 121 285 154 692.0 0 154 286 79 692.0 0 79 287 130 692.0 0 130 288 57 692.0 0 57 289 97 692.0 0 97 290 286 692.0 0 286 291 221 692.0 0 221 292 279 692.0 0 279 293 135 692.0 0 135 294 139 692.0 0 139 295 111 692.0 0 111 296 99 692.0 0 99 297 102 692.0 0 102 298 83 692.0 0 83 299 52 692.0 0 52 300 24 692.0 0 24 301 41 692.0 0 41 Finished in 21.48 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 654,789 Reads with adapters: 10,233 (1.6%) Reads that were too short: 85 (0.0%) Reads written (passing filters): 10,148 (1.5%) Total basepairs processed: 122,693,888 bp Total written (filtered): 2,789,103 bp (2.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10233 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 63 0.0 3 0 60 1 2 17 9885 0.0 3 9695 183 6 1 18 24 0.0 3 4 20 19 3 0.0 3 0 0 3 20 38 0.0 3 0 0 0 38 24 2 0.0 3 0 0 0 2 30 2 0.0 3 0 0 1 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 9 0.0 3 0 0 0 9 36 1 0.0 3 0 0 0 1 40 2 0.0 3 1 0 0 1 41 1 0.0 3 1 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 3 0.0 3 3 47 5 0.0 3 4 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 1 51 17 0.0 3 0 0 0 17 54 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 1 80 4 0.0 3 1 0 0 3 84 1 0.0 3 1 85 62 0.0 3 0 0 0 62 86 3 0.0 3 0 1 0 2 87 1 0.0 3 0 0 0 1 88 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 2 0.0 3 1 0 0 1 101 1 0.0 3 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 1 117 2 0.0 3 1 0 0 1 119 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 1 128 1 0.0 3 0 1 129 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 1 133 2 0.0 3 0 0 0 2 134 4 0.0 3 0 0 0 4 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 1 147 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 1 171 1 0.0 3 0 0 0 1 172 6 0.0 3 0 0 0 6 173 2 0.0 3 0 0 0 2 174 17 0.0 3 0 0 0 17 175 8 0.0 3 0 0 1 7 181 4 0.0 3 0 0 1 3 184 1 0.0 3 0 0 0 1 185 1 0.0 3 1 186 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 10,148 Reads with adapters: 54 (0.5%) Reads written (passing filters): 10,148 (100.0%) Total basepairs processed: 2,789,103 bp Total written (filtered): 2,788,927 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 54 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.7% C: 22.2% G: 16.7% T: 7.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 44 158.6 0 44 4 6 39.6 0 6 5 4 9.9 0 4