This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.69 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 179,087 (6.3%) Reads that were too short: 43,790 (1.5%) Reads written (passing filters): 135,297 (4.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 37,989,504 bp (4.5%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 179087 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2804 692.0 0 2804 7 48343 692.0 0 48343 8 2007 692.0 0 2007 9 67070 692.0 0 67070 10 71 692.0 0 71 11 43 692.0 0 43 12 46 692.0 0 46 13 19 692.0 0 19 14 24 692.0 0 24 15 53 692.0 0 53 16 29 692.0 0 29 17 51 692.0 0 51 18 24 692.0 0 24 19 56 692.0 0 56 20 24 692.0 0 24 21 36 692.0 0 36 22 25 692.0 0 25 23 27 692.0 0 27 24 25 692.0 0 25 25 26 692.0 0 26 26 19 692.0 0 19 27 18 692.0 0 18 28 24 692.0 0 24 29 49 692.0 0 49 30 133 692.0 0 133 31 41 692.0 0 41 32 53 692.0 0 53 33 46 692.0 0 46 34 32 692.0 0 32 35 25 692.0 0 25 36 99 692.0 0 99 37 30 692.0 0 30 38 36 692.0 0 36 39 32 692.0 0 32 40 43 692.0 0 43 41 27 692.0 0 27 42 31 692.0 0 31 43 41 692.0 0 41 44 25 692.0 0 25 45 31 692.0 0 31 46 22 692.0 0 22 47 65 692.0 0 65 48 46 692.0 0 46 49 69 692.0 0 69 50 52 692.0 0 52 51 62 692.0 0 62 52 32 692.0 0 32 53 47 692.0 0 47 54 37 692.0 0 37 55 43 692.0 0 43 56 45 692.0 0 45 57 358 692.0 0 358 58 48 692.0 0 48 59 81 692.0 0 81 60 39 692.0 0 39 61 43 692.0 0 43 62 39 692.0 0 39 63 58 692.0 0 58 64 70 692.0 0 70 65 29 692.0 0 29 66 48 692.0 0 48 67 27 692.0 0 27 68 104 692.0 0 104 69 44 692.0 0 44 70 58 692.0 0 58 71 98 692.0 0 98 72 202 692.0 0 202 73 161 692.0 0 161 74 24 692.0 0 24 75 56 692.0 0 56 76 20 692.0 0 20 77 55 692.0 0 55 78 56 692.0 0 56 79 40 692.0 0 40 80 34 692.0 0 34 81 44 692.0 0 44 82 39 692.0 0 39 83 67 692.0 0 67 84 50 692.0 0 50 85 57 692.0 0 57 86 57 692.0 0 57 87 58 692.0 0 58 88 41 692.0 0 41 89 35 692.0 0 35 90 66 692.0 0 66 91 43 692.0 0 43 92 46 692.0 0 46 93 72 692.0 0 72 94 73 692.0 0 73 95 66 692.0 0 66 96 37 692.0 0 37 97 33 692.0 0 33 98 52 692.0 0 52 99 34 692.0 0 34 100 24 692.0 0 24 101 32 692.0 0 32 102 41 692.0 0 41 103 32 692.0 0 32 104 67 692.0 0 67 105 27 692.0 0 27 106 49 692.0 0 49 107 32 692.0 0 32 108 33 692.0 0 33 109 36 692.0 0 36 110 34 692.0 0 34 111 56 692.0 0 56 112 36 692.0 0 36 113 30 692.0 0 30 114 30 692.0 0 30 115 32 692.0 0 32 116 41 692.0 0 41 117 82 692.0 0 82 118 48 692.0 0 48 119 50 692.0 0 50 120 58 692.0 0 58 121 38 692.0 0 38 122 41 692.0 0 41 123 248 692.0 0 248 124 86 692.0 0 86 125 448 692.0 0 448 126 114 692.0 0 114 127 61 692.0 0 61 128 100 692.0 0 100 129 96 692.0 0 96 130 1376 692.0 0 1376 131 368 692.0 0 368 132 984 692.0 0 984 133 254 692.0 0 254 134 300 692.0 0 300 135 135 692.0 0 135 136 82 692.0 0 82 137 358 692.0 0 358 138 847 692.0 0 847 139 167 692.0 0 167 140 37 692.0 0 37 141 39 692.0 0 39 142 57 692.0 0 57 143 65 692.0 0 65 144 34 692.0 0 34 145 21 692.0 0 21 146 37 692.0 0 37 147 32 692.0 0 32 148 26 692.0 0 26 149 43 692.0 0 43 150 64 692.0 0 64 151 124 692.0 0 124 152 58 692.0 0 58 153 61 692.0 0 61 154 48 692.0 0 48 155 36 692.0 0 36 156 33 692.0 0 33 157 70 692.0 0 70 158 37 692.0 0 37 159 34 692.0 0 34 160 19 692.0 0 19 161 29 692.0 0 29 162 29 692.0 0 29 163 24 692.0 0 24 164 23 692.0 0 23 165 36 692.0 0 36 166 43 692.0 0 43 167 55 692.0 0 55 168 37 692.0 0 37 169 28 692.0 0 28 170 29 692.0 0 29 171 24 692.0 0 24 172 21 692.0 0 21 173 27 692.0 0 27 174 41 692.0 0 41 175 51 692.0 0 51 176 32 692.0 0 32 177 106 692.0 0 106 178 91 692.0 0 91 179 602 692.0 0 602 180 44 692.0 0 44 181 26 692.0 0 26 182 36 692.0 0 36 183 28 692.0 0 28 184 34 692.0 0 34 185 40 692.0 0 40 186 76 692.0 0 76 187 139 692.0 0 139 188 91 692.0 0 91 189 97 692.0 0 97 190 49 692.0 0 49 191 22 692.0 0 22 192 27 692.0 0 27 193 29 692.0 0 29 194 25 692.0 0 25 195 34 692.0 0 34 196 47 692.0 0 47 197 59 692.0 0 59 198 49 692.0 0 49 199 55 692.0 0 55 200 48 692.0 0 48 201 46 692.0 0 46 202 37 692.0 0 37 203 73 692.0 0 73 204 144 692.0 0 144 205 64 692.0 0 64 206 53 692.0 0 53 207 34 692.0 0 34 208 38 692.0 0 38 209 48 692.0 0 48 210 54 692.0 0 54 211 96 692.0 0 96 212 823 692.0 0 823 213 410 692.0 0 410 214 1181 692.0 0 1181 215 63 692.0 0 63 216 173 692.0 0 173 217 93 692.0 0 93 218 213 692.0 0 213 219 39 692.0 0 39 220 54 692.0 0 54 221 32 692.0 0 32 222 37 692.0 0 37 223 54 692.0 0 54 224 49 692.0 0 49 225 34 692.0 0 34 226 39 692.0 0 39 227 35 692.0 0 35 228 64 692.0 0 64 229 52 692.0 0 52 230 58 692.0 0 58 231 73 692.0 0 73 232 63 692.0 0 63 233 98 692.0 0 98 234 87 692.0 0 87 235 173 692.0 0 173 236 423 692.0 0 423 237 517 692.0 0 517 238 706 692.0 0 706 239 176 692.0 0 176 240 65 692.0 0 65 241 272 692.0 0 272 242 169 692.0 0 169 243 1263 692.0 0 1263 244 705 692.0 0 705 245 941 692.0 0 941 246 596 692.0 0 596 247 172 692.0 0 172 248 103 692.0 0 103 249 193 692.0 0 193 250 170 692.0 0 170 251 233 692.0 0 233 252 148 692.0 0 148 253 65 692.0 0 65 254 131 692.0 0 131 255 203 692.0 0 203 256 218 692.0 0 218 257 405 692.0 0 405 258 355 692.0 0 355 259 272 692.0 0 272 260 460 692.0 0 460 261 449 692.0 0 449 262 536 692.0 0 536 263 302 692.0 0 302 264 94 692.0 0 94 265 504 692.0 0 504 266 110 692.0 0 110 267 104 692.0 0 104 268 121 692.0 0 121 269 66 692.0 0 66 270 77 692.0 0 77 271 144 692.0 0 144 272 301 692.0 0 301 273 456 692.0 0 456 274 170 692.0 0 170 275 304 692.0 0 304 276 326 692.0 0 326 277 299 692.0 0 299 278 124 692.0 0 124 279 217 692.0 0 217 280 345 692.0 0 345 281 415 692.0 0 415 282 271 692.0 0 271 283 276 692.0 0 276 284 394 692.0 0 394 285 565 692.0 0 565 286 10554 692.0 0 10554 287 5132 692.0 0 5132 288 4784 692.0 0 4784 289 94 692.0 0 94 290 87 692.0 0 87 291 86 692.0 0 86 292 68 692.0 0 68 293 84 692.0 0 84 294 98 692.0 0 98 295 124 692.0 0 124 296 327 692.0 0 327 297 578 692.0 0 578 298 621 692.0 0 621 299 361 692.0 0 361 300 165 692.0 0 165 301 58 692.0 0 58 Finished in 6.87 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 135,297 Reads with adapters: 49,903 (36.9%) Reads that were too short: 53 (0.0%) Reads written (passing filters): 49,850 (36.8%) Total basepairs processed: 37,989,504 bp Total written (filtered): 13,642,585 bp (35.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 49903 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 12 0.0 3 0 0 5 4 3 19 644 0.0 3 0 561 46 24 13 20 48798 0.0 4 46023 2232 415 106 22 21 111 0.0 4 15 84 3 6 3 22 8 0.0 4 1 0 2 3 2 23 103 0.0 4 0 1 0 80 22 24 1 0.0 4 0 0 0 1 25 1 0.0 4 0 1 26 1 0.0 4 1 27 1 0.0 4 1 28 3 0.0 4 0 0 0 0 3 34 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 1 67 1 0.0 4 0 1 69 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 1 90 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 98 3 0.0 4 0 0 0 0 3 99 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 127 147 0.0 4 0 0 0 0 147 129 1 0.0 4 0 0 0 1 130 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 6 0.0 4 1 0 0 0 5 159 2 0.0 4 0 0 0 0 2 160 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 1 175 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 3 0.0 4 0 0 0 0 3 223 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 234 5 0.0 4 0 0 0 0 5 235 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 1 252 1 0.0 4 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.72 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 49,850 Reads with adapters: 1,693 (3.4%) Reads written (passing filters): 49,850 (100.0%) Total basepairs processed: 13,642,585 bp Total written (filtered): 13,611,067 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1693 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 16.4% C: 16.3% G: 63.7% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 240 778.9 0 240 4 22 194.7 0 22 5 4 48.7 0 4 9 11 0.2 0 1 10 10 23 0.0 1 0 23 11 657 0.0 1 24 633 12 590 0.0 1 26 564 13 28 0.0 1 1 27 14 2 0.0 1 0 2 15 5 0.0 1 0 5 16 1 0.0 1 0 1 20 19 0.0 1 11 8 21 1 0.0 1 1 24 12 0.0 1 11 1 30 1 0.0 1 1 39 1 0.0 1 1 64 1 0.0 1 0 1 65 1 0.0 1 0 1 74 2 0.0 1 1 1 140 1 0.0 1 1 152 7 0.0 1 6 1 171 2 0.0 1 2 191 1 0.0 1 0 1 193 1 0.0 1 0 1 210 54 0.0 1 43 11 211 6 0.0 1 5 1