This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.45 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 186,112 (6.6%) Reads that were too short: 48,207 (1.7%) Reads written (passing filters): 137,905 (4.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 39,016,275 bp (4.6%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 186112 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3165 692.0 0 3165 7 68536 692.0 0 68536 8 1415 692.0 0 1415 9 51376 692.0 0 51376 10 75 692.0 0 75 11 37 692.0 0 37 12 48 692.0 0 48 13 35 692.0 0 35 14 40 692.0 0 40 15 42 692.0 0 42 16 35 692.0 0 35 17 26 692.0 0 26 18 45 692.0 0 45 19 46 692.0 0 46 20 21 692.0 0 21 21 23 692.0 0 23 22 33 692.0 0 33 23 32 692.0 0 32 24 26 692.0 0 26 25 30 692.0 0 30 26 30 692.0 0 30 27 25 692.0 0 25 28 39 692.0 0 39 29 31 692.0 0 31 30 37 692.0 0 37 31 27 692.0 0 27 32 50 692.0 0 50 33 40 692.0 0 40 34 56 692.0 0 56 35 36 692.0 0 36 36 109 692.0 0 109 37 53 692.0 0 53 38 43 692.0 0 43 39 31 692.0 0 31 40 46 692.0 0 46 41 36 692.0 0 36 42 42 692.0 0 42 43 41 692.0 0 41 44 37 692.0 0 37 45 38 692.0 0 38 46 32 692.0 0 32 47 55 692.0 0 55 48 35 692.0 0 35 49 64 692.0 0 64 50 60 692.0 0 60 51 51 692.0 0 51 52 57 692.0 0 57 53 51 692.0 0 51 54 59 692.0 0 59 55 39 692.0 0 39 56 43 692.0 0 43 57 411 692.0 0 411 58 51 692.0 0 51 59 48 692.0 0 48 60 47 692.0 0 47 61 48 692.0 0 48 62 47 692.0 0 47 63 71 692.0 0 71 64 53 692.0 0 53 65 37 692.0 0 37 66 45 692.0 0 45 67 38 692.0 0 38 68 92 692.0 0 92 69 55 692.0 0 55 70 25 692.0 0 25 71 101 692.0 0 101 72 204 692.0 0 204 73 140 692.0 0 140 74 22 692.0 0 22 75 50 692.0 0 50 76 23 692.0 0 23 77 46 692.0 0 46 78 45 692.0 0 45 79 37 692.0 0 37 80 36 692.0 0 36 81 51 692.0 0 51 82 35 692.0 0 35 83 70 692.0 0 70 84 31 692.0 0 31 85 43 692.0 0 43 86 55 692.0 0 55 87 17 692.0 0 17 88 48 692.0 0 48 89 34 692.0 0 34 90 51 692.0 0 51 91 48 692.0 0 48 92 51 692.0 0 51 93 58 692.0 0 58 94 56 692.0 0 56 95 51 692.0 0 51 96 27 692.0 0 27 97 32 692.0 0 32 98 38 692.0 0 38 99 24 692.0 0 24 100 30 692.0 0 30 101 18 692.0 0 18 102 31 692.0 0 31 103 35 692.0 0 35 104 30 692.0 0 30 105 23 692.0 0 23 106 37 692.0 0 37 107 20 692.0 0 20 108 26 692.0 0 26 109 40 692.0 0 40 110 28 692.0 0 28 111 37 692.0 0 37 112 24 692.0 0 24 113 22 692.0 0 22 114 26 692.0 0 26 115 30 692.0 0 30 116 39 692.0 0 39 117 71 692.0 0 71 118 41 692.0 0 41 119 37 692.0 0 37 120 51 692.0 0 51 121 27 692.0 0 27 122 25 692.0 0 25 123 240 692.0 0 240 124 70 692.0 0 70 125 474 692.0 0 474 126 88 692.0 0 88 127 59 692.0 0 59 128 96 692.0 0 96 129 75 692.0 0 75 130 1294 692.0 0 1294 131 232 692.0 0 232 132 928 692.0 0 928 133 179 692.0 0 179 134 256 692.0 0 256 135 129 692.0 0 129 136 48 692.0 0 48 137 249 692.0 0 249 138 738 692.0 0 738 139 201 692.0 0 201 140 23 692.0 0 23 141 19 692.0 0 19 142 24 692.0 0 24 143 38 692.0 0 38 144 22 692.0 0 22 145 19 692.0 0 19 146 14 692.0 0 14 147 23 692.0 0 23 148 25 692.0 0 25 149 38 692.0 0 38 150 35 692.0 0 35 151 57 692.0 0 57 152 52 692.0 0 52 153 42 692.0 0 42 154 43 692.0 0 43 155 26 692.0 0 26 156 18 692.0 0 18 157 61 692.0 0 61 158 23 692.0 0 23 159 46 692.0 0 46 160 25 692.0 0 25 161 15 692.0 0 15 162 22 692.0 0 22 163 19 692.0 0 19 164 31 692.0 0 31 165 26 692.0 0 26 166 18 692.0 0 18 167 45 692.0 0 45 168 28 692.0 0 28 169 19 692.0 0 19 170 18 692.0 0 18 171 13 692.0 0 13 172 20 692.0 0 20 173 34 692.0 0 34 174 29 692.0 0 29 175 49 692.0 0 49 176 25 692.0 0 25 177 112 692.0 0 112 178 94 692.0 0 94 179 783 692.0 0 783 180 49 692.0 0 49 181 16 692.0 0 16 182 23 692.0 0 23 183 23 692.0 0 23 184 31 692.0 0 31 185 21 692.0 0 21 186 32 692.0 0 32 187 38 692.0 0 38 188 41 692.0 0 41 189 36 692.0 0 36 190 39 692.0 0 39 191 23 692.0 0 23 192 37 692.0 0 37 193 23 692.0 0 23 194 24 692.0 0 24 195 19 692.0 0 19 196 25 692.0 0 25 197 29 692.0 0 29 198 41 692.0 0 41 199 34 692.0 0 34 200 32 692.0 0 32 201 21 692.0 0 21 202 22 692.0 0 22 203 27 692.0 0 27 204 54 692.0 0 54 205 20 692.0 0 20 206 25 692.0 0 25 207 22 692.0 0 22 208 21 692.0 0 21 209 25 692.0 0 25 210 28 692.0 0 28 211 45 692.0 0 45 212 605 692.0 0 605 213 164 692.0 0 164 214 476 692.0 0 476 215 31 692.0 0 31 216 33 692.0 0 33 217 47 692.0 0 47 218 113 692.0 0 113 219 29 692.0 0 29 220 44 692.0 0 44 221 27 692.0 0 27 222 26 692.0 0 26 223 25 692.0 0 25 224 12 692.0 0 12 225 24 692.0 0 24 226 24 692.0 0 24 227 31 692.0 0 31 228 28 692.0 0 28 229 29 692.0 0 29 230 24 692.0 0 24 231 25 692.0 0 25 232 35 692.0 0 35 233 32 692.0 0 32 234 43 692.0 0 43 235 110 692.0 0 110 236 116 692.0 0 116 237 225 692.0 0 225 238 164 692.0 0 164 239 54 692.0 0 54 240 34 692.0 0 34 241 43 692.0 0 43 242 46 692.0 0 46 243 264 692.0 0 264 244 249 692.0 0 249 245 219 692.0 0 219 246 301 692.0 0 301 247 88 692.0 0 88 248 62 692.0 0 62 249 78 692.0 0 78 250 73 692.0 0 73 251 79 692.0 0 79 252 43 692.0 0 43 253 35 692.0 0 35 254 40 692.0 0 40 255 71 692.0 0 71 256 80 692.0 0 80 257 208 692.0 0 208 258 156 692.0 0 156 259 120 692.0 0 120 260 537 692.0 0 537 261 465 692.0 0 465 262 730 692.0 0 730 263 358 692.0 0 358 264 42 692.0 0 42 265 570 692.0 0 570 266 63 692.0 0 63 267 60 692.0 0 60 268 55 692.0 0 55 269 49 692.0 0 49 270 57 692.0 0 57 271 138 692.0 0 138 272 363 692.0 0 363 273 660 692.0 0 660 274 161 692.0 0 161 275 201 692.0 0 201 276 342 692.0 0 342 277 172 692.0 0 172 278 129 692.0 0 129 279 303 692.0 0 303 280 421 692.0 0 421 281 426 692.0 0 426 282 312 692.0 0 312 283 343 692.0 0 343 284 411 692.0 0 411 285 579 692.0 0 579 286 16837 692.0 0 16837 287 8997 692.0 0 8997 288 7970 692.0 0 7970 289 45 692.0 0 45 290 74 692.0 0 74 291 49 692.0 0 49 292 40 692.0 0 40 293 57 692.0 0 57 294 46 692.0 0 46 295 71 692.0 0 71 296 125 692.0 0 125 297 227 692.0 0 227 298 270 692.0 0 270 299 143 692.0 0 143 300 113 692.0 0 113 301 22 692.0 0 22 Finished in 6.46 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 137,905 Reads with adapters: 53,423 (38.7%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 53,383 (38.7%) Total basepairs processed: 39,016,275 bp Total written (filtered): 14,674,052 bp (37.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 53423 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 11 0.0 3 0 0 10 1 16 361 0.0 3 0 345 12 4 17 52638 0.0 3 51419 1177 39 3 18 142 0.0 3 11 123 7 1 19 15 0.0 3 2 0 7 6 20 153 0.0 3 0 0 0 153 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 1 29 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 17 0.0 3 0 0 0 17 63 1 0.0 3 0 0 1 64 2 0.0 3 0 0 1 1 66 3 0.0 3 0 0 3 67 1 0.0 3 1 68 1 0.0 3 1 73 1 0.0 3 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 1 82 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 98 3 0.0 3 0 0 0 3 100 1 0.0 3 1 112 3 0.0 3 3 114 1 0.0 3 1 128 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 151 1 0.0 3 1 161 1 0.0 3 1 164 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 191 2 0.0 3 0 0 0 2 192 14 0.0 3 0 0 0 14 194 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 1 231 1 0.0 3 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.89 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 53,383 Reads with adapters: 1,109 (2.1%) Reads written (passing filters): 53,383 (100.0%) Total basepairs processed: 14,674,052 bp Total written (filtered): 14,670,706 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1109 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.0% C: 6.1% G: 28.7% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1092 834.1 0 1092 4 16 208.5 0 16 6 1 13.0 0 1