This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.50 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 43,554 (1.5%) Reads that were too short: 19,116 (0.7%) Reads written (passing filters): 24,438 (0.9%) Total basepairs processed: 853,219,115 bp Total written (filtered): 4,841,700 bp (0.6%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 43554 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 74 692.0 0 74 7 142 692.0 0 142 8 1832 692.0 0 1832 9 1545 692.0 0 1545 10 42 692.0 0 42 11 34 692.0 0 34 12 35 692.0 0 35 13 45 692.0 0 45 14 44 692.0 0 44 15 43 692.0 0 43 16 52 692.0 0 52 17 49 692.0 0 49 18 37 692.0 0 37 19 36 692.0 0 36 20 34 692.0 0 34 21 45 692.0 0 45 22 41 692.0 0 41 23 51 692.0 0 51 24 40 692.0 0 40 25 42 692.0 0 42 26 43 692.0 0 43 27 41 692.0 0 41 28 33 692.0 0 33 29 39 692.0 0 39 30 41 692.0 0 41 31 42 692.0 0 42 32 59 692.0 0 59 33 43 692.0 0 43 34 45 692.0 0 45 35 52 692.0 0 52 36 38 692.0 0 38 37 48 692.0 0 48 38 135 692.0 0 135 39 50 692.0 0 50 40 80 692.0 0 80 41 61 692.0 0 61 42 44 692.0 0 44 43 51 692.0 0 51 44 35 692.0 0 35 45 54 692.0 0 54 46 34 692.0 0 34 47 50 692.0 0 50 48 29 692.0 0 29 49 54 692.0 0 54 50 40 692.0 0 40 51 57 692.0 0 57 52 56 692.0 0 56 53 77 692.0 0 77 54 44 692.0 0 44 55 51 692.0 0 51 56 35 692.0 0 35 57 42 692.0 0 42 58 42 692.0 0 42 59 36 692.0 0 36 60 55 692.0 0 55 61 60 692.0 0 60 62 39 692.0 0 39 63 51 692.0 0 51 64 60 692.0 0 60 65 61 692.0 0 61 66 69 692.0 0 69 67 104 692.0 0 104 68 75 692.0 0 75 69 50 692.0 0 50 70 51 692.0 0 51 71 60 692.0 0 60 72 42 692.0 0 42 73 60 692.0 0 60 74 83 692.0 0 83 75 77 692.0 0 77 76 88 692.0 0 88 77 51 692.0 0 51 78 66 692.0 0 66 79 64 692.0 0 64 80 61 692.0 0 61 81 54 692.0 0 54 82 87 692.0 0 87 83 77 692.0 0 77 84 62 692.0 0 62 85 40 692.0 0 40 86 66 692.0 0 66 87 64 692.0 0 64 88 65 692.0 0 65 89 77 692.0 0 77 90 72 692.0 0 72 91 132 692.0 0 132 92 66 692.0 0 66 93 55 692.0 0 55 94 94 692.0 0 94 95 210 692.0 0 210 96 105 692.0 0 105 97 968 692.0 0 968 98 766 692.0 0 766 99 695 692.0 0 695 100 1082 692.0 0 1082 101 77 692.0 0 77 102 41 692.0 0 41 103 48 692.0 0 48 104 53 692.0 0 53 105 58 692.0 0 58 106 63 692.0 0 63 107 86 692.0 0 86 108 130 692.0 0 130 109 151 692.0 0 151 110 114 692.0 0 114 111 586 692.0 0 586 112 1547 692.0 0 1547 113 532 692.0 0 532 114 55 692.0 0 55 115 52 692.0 0 52 116 54 692.0 0 54 117 72 692.0 0 72 118 35 692.0 0 35 119 56 692.0 0 56 120 54 692.0 0 54 121 34 692.0 0 34 122 52 692.0 0 52 123 55 692.0 0 55 124 41 692.0 0 41 125 37 692.0 0 37 126 67 692.0 0 67 127 52 692.0 0 52 128 63 692.0 0 63 129 100 692.0 0 100 130 103 692.0 0 103 131 82 692.0 0 82 132 79 692.0 0 79 133 68 692.0 0 68 134 207 692.0 0 207 135 81 692.0 0 81 136 98 692.0 0 98 137 73 692.0 0 73 138 78 692.0 0 78 139 35 692.0 0 35 140 103 692.0 0 103 141 104 692.0 0 104 142 56 692.0 0 56 143 58 692.0 0 58 144 64 692.0 0 64 145 64 692.0 0 64 146 118 692.0 0 118 147 84 692.0 0 84 148 100 692.0 0 100 149 84 692.0 0 84 150 65 692.0 0 65 151 71 692.0 0 71 152 64 692.0 0 64 153 63 692.0 0 63 154 158 692.0 0 158 155 207 692.0 0 207 156 481 692.0 0 481 157 178 692.0 0 178 158 54 692.0 0 54 159 40 692.0 0 40 160 61 692.0 0 61 161 56 692.0 0 56 162 55 692.0 0 55 163 58 692.0 0 58 164 71 692.0 0 71 165 65 692.0 0 65 166 302 692.0 0 302 167 232 692.0 0 232 168 253 692.0 0 253 169 43 692.0 0 43 170 60 692.0 0 60 171 45 692.0 0 45 172 45 692.0 0 45 173 36 692.0 0 36 174 61 692.0 0 61 175 57 692.0 0 57 176 51 692.0 0 51 177 61 692.0 0 61 178 55 692.0 0 55 179 64 692.0 0 64 180 64 692.0 0 64 181 93 692.0 0 93 182 66 692.0 0 66 183 49 692.0 0 49 184 61 692.0 0 61 185 76 692.0 0 76 186 92 692.0 0 92 187 64 692.0 0 64 188 62 692.0 0 62 189 61 692.0 0 61 190 67 692.0 0 67 191 59 692.0 0 59 192 91 692.0 0 91 193 297 692.0 0 297 194 380 692.0 0 380 195 434 692.0 0 434 196 220 692.0 0 220 197 164 692.0 0 164 198 106 692.0 0 106 199 129 692.0 0 129 200 256 692.0 0 256 201 223 692.0 0 223 202 212 692.0 0 212 203 62 692.0 0 62 204 62 692.0 0 62 205 61 692.0 0 61 206 73 692.0 0 73 207 116 692.0 0 116 208 107 692.0 0 107 209 77 692.0 0 77 210 88 692.0 0 88 211 65 692.0 0 65 212 59 692.0 0 59 213 70 692.0 0 70 214 85 692.0 0 85 215 83 692.0 0 83 216 135 692.0 0 135 217 140 692.0 0 140 218 71 692.0 0 71 219 93 692.0 0 93 220 634 692.0 0 634 221 535 692.0 0 535 222 1490 692.0 0 1490 223 248 692.0 0 248 224 69 692.0 0 69 225 58 692.0 0 58 226 58 692.0 0 58 227 61 692.0 0 61 228 69 692.0 0 69 229 69 692.0 0 69 230 53 692.0 0 53 231 66 692.0 0 66 232 71 692.0 0 71 233 63 692.0 0 63 234 51 692.0 0 51 235 65 692.0 0 65 236 90 692.0 0 90 237 129 692.0 0 129 238 171 692.0 0 171 239 269 692.0 0 269 240 186 692.0 0 186 241 188 692.0 0 188 242 232 692.0 0 232 243 524 692.0 0 524 244 243 692.0 0 243 245 351 692.0 0 351 246 108 692.0 0 108 247 68 692.0 0 68 248 84 692.0 0 84 249 106 692.0 0 106 250 106 692.0 0 106 251 126 692.0 0 126 252 181 692.0 0 181 253 182 692.0 0 182 254 541 692.0 0 541 255 263 692.0 0 263 256 719 692.0 0 719 257 102 692.0 0 102 258 109 692.0 0 109 259 175 692.0 0 175 260 104 692.0 0 104 261 148 692.0 0 148 262 740 692.0 0 740 263 916 692.0 0 916 264 1768 692.0 0 1768 265 109 692.0 0 109 266 72 692.0 0 72 267 76 692.0 0 76 268 84 692.0 0 84 269 143 692.0 0 143 270 139 692.0 0 139 271 114 692.0 0 114 272 97 692.0 0 97 273 110 692.0 0 110 274 172 692.0 0 172 275 144 692.0 0 144 276 202 692.0 0 202 277 81 692.0 0 81 278 90 692.0 0 90 279 133 692.0 0 133 280 198 692.0 0 198 281 238 692.0 0 238 282 160 692.0 0 160 283 122 692.0 0 122 284 88 692.0 0 88 285 84 692.0 0 84 286 67 692.0 0 67 287 74 692.0 0 74 288 111 692.0 0 111 289 203 692.0 0 203 290 144 692.0 0 144 291 154 692.0 0 154 292 146 692.0 0 146 293 175 692.0 0 175 294 230 692.0 0 230 295 155 692.0 0 155 296 75 692.0 0 75 297 114 692.0 0 114 298 125 692.0 0 125 299 110 692.0 0 110 300 137 692.0 0 137 301 192 692.0 0 192 Finished in 1.04 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 24,438 Reads with adapters: 1,635 (6.7%) Reads that were too short: 18 (0.1%) Reads written (passing filters): 1,617 (6.6%) Total basepairs processed: 4,841,700 bp Total written (filtered): 437,797 bp (9.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1635 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 1 19 30 0.0 3 0 27 0 2 1 20 1557 0.0 4 1427 105 20 4 1 21 3 0.0 4 1 2 22 2 0.0 4 0 0 1 0 1 23 5 0.0 4 0 0 0 3 2 24 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 1 45 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 127 10 0.0 4 0 0 0 0 10 142 2 0.0 4 0 0 0 0 2 164 2 0.0 4 0 0 0 0 2 176 1 0.0 4 0 0 0 1 177 1 0.0 4 0 0 0 1 203 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 1,617 Reads with adapters: 380 (23.5%) Reads written (passing filters): 1,617 (100.0%) Total basepairs processed: 437,797 bp Total written (filtered): 431,429 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 380 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.1% C: 9.7% G: 82.1% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 23 0.0 0 1 22 10 307 0.0 1 28 279 11 34 0.0 1 3 31 12 1 0.0 1 0 1 13 4 0.0 1 0 4 208 2 0.0 1 1 1 209 1 0.0 1 1 228 1 0.0 1 1 257 6 0.0 1 5 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.