This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.36 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 27,611 (1.0%) Reads that were too short: 11,709 (0.4%) Reads written (passing filters): 15,902 (0.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 3,312,183 bp (0.4%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 27611 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 44 692.0 0 44 7 97 692.0 0 97 8 1635 692.0 0 1635 9 1838 692.0 0 1838 10 25 692.0 0 25 11 25 692.0 0 25 12 39 692.0 0 39 13 44 692.0 0 44 14 14 692.0 0 14 15 26 692.0 0 26 16 24 692.0 0 24 17 20 692.0 0 20 18 19 692.0 0 19 19 34 692.0 0 34 20 18 692.0 0 18 21 25 692.0 0 25 22 22 692.0 0 22 23 33 692.0 0 33 24 39 692.0 0 39 25 19 692.0 0 19 26 28 692.0 0 28 27 18 692.0 0 18 28 31 692.0 0 31 29 17 692.0 0 17 30 31 692.0 0 31 31 25 692.0 0 25 32 26 692.0 0 26 33 27 692.0 0 27 34 30 692.0 0 30 35 32 692.0 0 32 36 42 692.0 0 42 37 26 692.0 0 26 38 109 692.0 0 109 39 21 692.0 0 21 40 42 692.0 0 42 41 31 692.0 0 31 42 42 692.0 0 42 43 24 692.0 0 24 44 22 692.0 0 22 45 22 692.0 0 22 46 27 692.0 0 27 47 25 692.0 0 25 48 26 692.0 0 26 49 23 692.0 0 23 50 28 692.0 0 28 51 37 692.0 0 37 52 35 692.0 0 35 53 51 692.0 0 51 54 23 692.0 0 23 55 28 692.0 0 28 56 28 692.0 0 28 57 31 692.0 0 31 58 23 692.0 0 23 59 29 692.0 0 29 60 33 692.0 0 33 61 40 692.0 0 40 62 25 692.0 0 25 63 37 692.0 0 37 64 30 692.0 0 30 65 26 692.0 0 26 66 41 692.0 0 41 67 46 692.0 0 46 68 37 692.0 0 37 69 28 692.0 0 28 70 24 692.0 0 24 71 28 692.0 0 28 72 38 692.0 0 38 73 26 692.0 0 26 74 48 692.0 0 48 75 47 692.0 0 47 76 58 692.0 0 58 77 19 692.0 0 19 78 31 692.0 0 31 79 39 692.0 0 39 80 40 692.0 0 40 81 26 692.0 0 26 82 29 692.0 0 29 83 30 692.0 0 30 84 35 692.0 0 35 85 34 692.0 0 34 86 17 692.0 0 17 87 40 692.0 0 40 88 30 692.0 0 30 89 39 692.0 0 39 90 37 692.0 0 37 91 93 692.0 0 93 92 44 692.0 0 44 93 43 692.0 0 43 94 48 692.0 0 48 95 167 692.0 0 167 96 62 692.0 0 62 97 335 692.0 0 335 98 321 692.0 0 321 99 261 692.0 0 261 100 334 692.0 0 334 101 46 692.0 0 46 102 28 692.0 0 28 103 38 692.0 0 38 104 41 692.0 0 41 105 46 692.0 0 46 106 43 692.0 0 43 107 40 692.0 0 40 108 67 692.0 0 67 109 76 692.0 0 76 110 64 692.0 0 64 111 573 692.0 0 573 112 1743 692.0 0 1743 113 467 692.0 0 467 114 28 692.0 0 28 115 22 692.0 0 22 116 28 692.0 0 28 117 41 692.0 0 41 118 37 692.0 0 37 119 33 692.0 0 33 120 29 692.0 0 29 121 29 692.0 0 29 122 30 692.0 0 30 123 39 692.0 0 39 124 34 692.0 0 34 125 34 692.0 0 34 126 33 692.0 0 33 127 29 692.0 0 29 128 48 692.0 0 48 129 39 692.0 0 39 130 81 692.0 0 81 131 38 692.0 0 38 132 42 692.0 0 42 133 33 692.0 0 33 134 73 692.0 0 73 135 43 692.0 0 43 136 49 692.0 0 49 137 37 692.0 0 37 138 31 692.0 0 31 139 34 692.0 0 34 140 36 692.0 0 36 141 51 692.0 0 51 142 35 692.0 0 35 143 26 692.0 0 26 144 39 692.0 0 39 145 31 692.0 0 31 146 78 692.0 0 78 147 57 692.0 0 57 148 38 692.0 0 38 149 49 692.0 0 49 150 42 692.0 0 42 151 27 692.0 0 27 152 30 692.0 0 30 153 37 692.0 0 37 154 77 692.0 0 77 155 110 692.0 0 110 156 243 692.0 0 243 157 100 692.0 0 100 158 28 692.0 0 28 159 23 692.0 0 23 160 29 692.0 0 29 161 36 692.0 0 36 162 38 692.0 0 38 163 27 692.0 0 27 164 47 692.0 0 47 165 35 692.0 0 35 166 169 692.0 0 169 167 121 692.0 0 121 168 105 692.0 0 105 169 24 692.0 0 24 170 35 692.0 0 35 171 26 692.0 0 26 172 26 692.0 0 26 173 28 692.0 0 28 174 25 692.0 0 25 175 26 692.0 0 26 176 39 692.0 0 39 177 36 692.0 0 36 178 35 692.0 0 35 179 46 692.0 0 46 180 28 692.0 0 28 181 84 692.0 0 84 182 38 692.0 0 38 183 40 692.0 0 40 184 35 692.0 0 35 185 33 692.0 0 33 186 45 692.0 0 45 187 33 692.0 0 33 188 40 692.0 0 40 189 39 692.0 0 39 190 37 692.0 0 37 191 40 692.0 0 40 192 49 692.0 0 49 193 100 692.0 0 100 194 211 692.0 0 211 195 291 692.0 0 291 196 133 692.0 0 133 197 63 692.0 0 63 198 61 692.0 0 61 199 68 692.0 0 68 200 117 692.0 0 117 201 75 692.0 0 75 202 74 692.0 0 74 203 37 692.0 0 37 204 39 692.0 0 39 205 38 692.0 0 38 206 35 692.0 0 35 207 58 692.0 0 58 208 51 692.0 0 51 209 34 692.0 0 34 210 48 692.0 0 48 211 32 692.0 0 32 212 30 692.0 0 30 213 43 692.0 0 43 214 47 692.0 0 47 215 47 692.0 0 47 216 75 692.0 0 75 217 60 692.0 0 60 218 49 692.0 0 49 219 40 692.0 0 40 220 253 692.0 0 253 221 192 692.0 0 192 222 530 692.0 0 530 223 139 692.0 0 139 224 35 692.0 0 35 225 43 692.0 0 43 226 42 692.0 0 42 227 47 692.0 0 47 228 46 692.0 0 46 229 40 692.0 0 40 230 38 692.0 0 38 231 31 692.0 0 31 232 37 692.0 0 37 233 44 692.0 0 44 234 41 692.0 0 41 235 48 692.0 0 48 236 53 692.0 0 53 237 136 692.0 0 136 238 146 692.0 0 146 239 249 692.0 0 249 240 125 692.0 0 125 241 81 692.0 0 81 242 114 692.0 0 114 243 207 692.0 0 207 244 106 692.0 0 106 245 147 692.0 0 147 246 65 692.0 0 65 247 49 692.0 0 49 248 58 692.0 0 58 249 81 692.0 0 81 250 60 692.0 0 60 251 69 692.0 0 69 252 134 692.0 0 134 253 114 692.0 0 114 254 206 692.0 0 206 255 135 692.0 0 135 256 282 692.0 0 282 257 64 692.0 0 64 258 48 692.0 0 48 259 166 692.0 0 166 260 63 692.0 0 63 261 79 692.0 0 79 262 733 692.0 0 733 263 872 692.0 0 872 264 1482 692.0 0 1482 265 59 692.0 0 59 266 40 692.0 0 40 267 33 692.0 0 33 268 39 692.0 0 39 269 104 692.0 0 104 270 75 692.0 0 75 271 52 692.0 0 52 272 59 692.0 0 59 273 58 692.0 0 58 274 81 692.0 0 81 275 80 692.0 0 80 276 89 692.0 0 89 277 37 692.0 0 37 278 65 692.0 0 65 279 89 692.0 0 89 280 106 692.0 0 106 281 114 692.0 0 114 282 79 692.0 0 79 283 51 692.0 0 51 284 47 692.0 0 47 285 47 692.0 0 47 286 60 692.0 0 60 287 36 692.0 0 36 288 84 692.0 0 84 289 142 692.0 0 142 290 116 692.0 0 116 291 130 692.0 0 130 292 93 692.0 0 93 293 162 692.0 0 162 294 224 692.0 0 224 295 186 692.0 0 186 296 70 692.0 0 70 297 89 692.0 0 89 298 71 692.0 0 71 299 95 692.0 0 95 300 86 692.0 0 86 301 94 692.0 0 94 Finished in 0.61 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 15,902 Reads with adapters: 1,746 (11.0%) Reads that were too short: 65 (0.4%) Reads written (passing filters): 1,681 (10.6%) Total basepairs processed: 3,312,183 bp Total written (filtered): 452,861 bp (13.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1746 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 13 0.0 3 0 13 17 1581 0.0 3 1561 19 1 18 9 0.0 3 2 5 1 1 20 9 0.0 3 1 0 0 8 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 1 37 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 61 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 76 13 0.0 3 0 0 0 13 77 22 0.0 3 0 0 0 22 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 4 0.0 3 1 0 1 2 83 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 9 0.0 3 0 0 0 9 94 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 18 0.0 3 0 0 0 18 189 36 0.0 3 0 0 0 36 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 1,681 Reads with adapters: 4 (0.2%) Reads written (passing filters): 1,681 (100.0%) Total basepairs processed: 452,861 bp Total written (filtered): 452,849 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 25.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 26.3 0 4