This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.27 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 179,532 (6.3%) Reads that were too short: 14,309 (0.5%) Reads written (passing filters): 165,223 (5.8%) Total basepairs processed: 853,219,115 bp Total written (filtered): 37,384,495 bp (4.4%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 179532 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 307 692.0 0 307 7 3127 692.0 0 3127 8 4117 692.0 0 4117 9 61 692.0 0 61 10 46 692.0 0 46 11 87 692.0 0 87 12 68 692.0 0 68 13 66 692.0 0 66 14 60 692.0 0 60 15 75 692.0 0 75 16 75 692.0 0 75 17 67 692.0 0 67 18 79 692.0 0 79 19 128 692.0 0 128 20 92 692.0 0 92 21 85 692.0 0 85 22 100 692.0 0 100 23 86 692.0 0 86 24 85 692.0 0 85 25 77 692.0 0 77 26 120 692.0 0 120 27 70 692.0 0 70 28 137 692.0 0 137 29 327 692.0 0 327 30 318 692.0 0 318 31 59 692.0 0 59 32 59 692.0 0 59 33 67 692.0 0 67 34 68 692.0 0 68 35 70 692.0 0 70 36 108 692.0 0 108 37 115 692.0 0 115 38 192 692.0 0 192 39 422 692.0 0 422 40 151 692.0 0 151 41 210 692.0 0 210 42 116 692.0 0 116 43 137 692.0 0 137 44 283 692.0 0 283 45 154 692.0 0 154 46 169 692.0 0 169 47 170 692.0 0 170 48 166 692.0 0 166 49 238 692.0 0 238 50 236 692.0 0 236 51 397 692.0 0 397 52 619 692.0 0 619 53 844 692.0 0 844 54 1901 692.0 0 1901 55 2677 692.0 0 2677 56 52062 692.0 0 52062 57 30737 692.0 0 30737 58 30564 692.0 0 30564 59 187 692.0 0 187 60 61 692.0 0 61 61 60 692.0 0 60 62 140 692.0 0 140 63 81 692.0 0 81 64 119 692.0 0 119 65 60 692.0 0 60 66 63 692.0 0 63 67 61 692.0 0 61 68 63 692.0 0 63 69 97 692.0 0 97 70 63 692.0 0 63 71 90 692.0 0 90 72 70 692.0 0 70 73 85 692.0 0 85 74 88 692.0 0 88 75 58 692.0 0 58 76 69 692.0 0 69 77 57 692.0 0 57 78 56 692.0 0 56 79 73 692.0 0 73 80 58 692.0 0 58 81 63 692.0 0 63 82 72 692.0 0 72 83 97 692.0 0 97 84 82 692.0 0 82 85 71 692.0 0 71 86 80 692.0 0 80 87 120 692.0 0 120 88 145 692.0 0 145 89 112 692.0 0 112 90 57 692.0 0 57 91 68 692.0 0 68 92 63 692.0 0 63 93 63 692.0 0 63 94 66 692.0 0 66 95 43 692.0 0 43 96 59 692.0 0 59 97 70 692.0 0 70 98 53 692.0 0 53 99 114 692.0 0 114 100 90 692.0 0 90 101 56 692.0 0 56 102 53 692.0 0 53 103 166 692.0 0 166 104 98 692.0 0 98 105 146 692.0 0 146 106 60 692.0 0 60 107 344 692.0 0 344 108 68 692.0 0 68 109 51 692.0 0 51 110 67 692.0 0 67 111 94 692.0 0 94 112 95 692.0 0 95 113 82 692.0 0 82 114 62 692.0 0 62 115 48 692.0 0 48 116 73 692.0 0 73 117 51 692.0 0 51 118 54 692.0 0 54 119 70 692.0 0 70 120 72 692.0 0 72 121 98 692.0 0 98 122 45 692.0 0 45 123 65 692.0 0 65 124 114 692.0 0 114 125 144 692.0 0 144 126 263 692.0 0 263 127 86 692.0 0 86 128 183 692.0 0 183 129 133 692.0 0 133 130 74 692.0 0 74 131 207 692.0 0 207 132 79 692.0 0 79 133 61 692.0 0 61 134 80 692.0 0 80 135 89 692.0 0 89 136 131 692.0 0 131 137 43 692.0 0 43 138 84 692.0 0 84 139 285 692.0 0 285 140 88 692.0 0 88 141 79 692.0 0 79 142 103 692.0 0 103 143 94 692.0 0 94 144 83 692.0 0 83 145 109 692.0 0 109 146 128 692.0 0 128 147 711 692.0 0 711 148 976 692.0 0 976 149 626 692.0 0 626 150 468 692.0 0 468 151 281 692.0 0 281 152 253 692.0 0 253 153 74 692.0 0 74 154 109 692.0 0 109 155 50 692.0 0 50 156 90 692.0 0 90 157 62 692.0 0 62 158 58 692.0 0 58 159 77 692.0 0 77 160 205 692.0 0 205 161 106 692.0 0 106 162 89 692.0 0 89 163 98 692.0 0 98 164 80 692.0 0 80 165 135 692.0 0 135 166 110 692.0 0 110 167 1364 692.0 0 1364 168 615 692.0 0 615 169 909 692.0 0 909 170 94 692.0 0 94 171 107 692.0 0 107 172 131 692.0 0 131 173 364 692.0 0 364 174 127 692.0 0 127 175 351 692.0 0 351 176 157 692.0 0 157 177 184 692.0 0 184 178 89 692.0 0 89 179 56 692.0 0 56 180 89 692.0 0 89 181 93 692.0 0 93 182 76 692.0 0 76 183 132 692.0 0 132 184 118 692.0 0 118 185 104 692.0 0 104 186 188 692.0 0 188 187 224 692.0 0 224 188 311 692.0 0 311 189 126 692.0 0 126 190 126 692.0 0 126 191 129 692.0 0 129 192 139 692.0 0 139 193 220 692.0 0 220 194 167 692.0 0 167 195 451 692.0 0 451 196 367 692.0 0 367 197 591 692.0 0 591 198 369 692.0 0 369 199 716 692.0 0 716 200 7168 692.0 0 7168 201 3157 692.0 0 3157 202 4432 692.0 0 4432 203 111 692.0 0 111 204 63 692.0 0 63 205 55 692.0 0 55 206 61 692.0 0 61 207 56 692.0 0 56 208 65 692.0 0 65 209 53 692.0 0 53 210 53 692.0 0 53 211 64 692.0 0 64 212 63 692.0 0 63 213 55 692.0 0 55 214 58 692.0 0 58 215 116 692.0 0 116 216 70 692.0 0 70 217 59 692.0 0 59 218 68 692.0 0 68 219 129 692.0 0 129 220 93 692.0 0 93 221 99 692.0 0 99 222 53 692.0 0 53 223 48 692.0 0 48 224 54 692.0 0 54 225 56 692.0 0 56 226 55 692.0 0 55 227 56 692.0 0 56 228 81 692.0 0 81 229 157 692.0 0 157 230 185 692.0 0 185 231 236 692.0 0 236 232 465 692.0 0 465 233 200 692.0 0 200 234 227 692.0 0 227 235 64 692.0 0 64 236 60 692.0 0 60 237 59 692.0 0 59 238 93 692.0 0 93 239 74 692.0 0 74 240 86 692.0 0 86 241 89 692.0 0 89 242 56 692.0 0 56 243 88 692.0 0 88 244 51 692.0 0 51 245 51 692.0 0 51 246 58 692.0 0 58 247 47 692.0 0 47 248 73 692.0 0 73 249 69 692.0 0 69 250 68 692.0 0 68 251 68 692.0 0 68 252 71 692.0 0 71 253 41 692.0 0 41 254 49 692.0 0 49 255 51 692.0 0 51 256 63 692.0 0 63 257 114 692.0 0 114 258 55 692.0 0 55 259 69 692.0 0 69 260 75 692.0 0 75 261 116 692.0 0 116 262 47 692.0 0 47 263 56 692.0 0 56 264 61 692.0 0 61 265 71 692.0 0 71 266 56 692.0 0 56 267 75 692.0 0 75 268 127 692.0 0 127 269 106 692.0 0 106 270 158 692.0 0 158 271 89 692.0 0 89 272 75 692.0 0 75 273 144 692.0 0 144 274 97 692.0 0 97 275 128 692.0 0 128 276 124 692.0 0 124 277 110 692.0 0 110 278 211 692.0 0 211 279 91 692.0 0 91 280 480 692.0 0 480 281 219 692.0 0 219 282 177 692.0 0 177 283 377 692.0 0 377 284 47 692.0 0 47 285 54 692.0 0 54 286 49 692.0 0 49 287 105 692.0 0 105 288 121 692.0 0 121 289 165 692.0 0 165 290 152 692.0 0 152 291 73 692.0 0 73 292 72 692.0 0 72 293 124 692.0 0 124 294 91 692.0 0 91 295 90 692.0 0 90 296 80 692.0 0 80 297 83 692.0 0 83 298 65 692.0 0 65 299 52 692.0 0 52 300 139 692.0 0 139 301 114 692.0 0 114 Finished in 6.29 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 165,223 Reads with adapters: 3,217 (1.9%) Reads that were too short: 57 (0.0%) Reads written (passing filters): 3,160 (1.9%) Total basepairs processed: 37,384,495 bp Total written (filtered): 864,937 bp (2.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3217 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 23 0.0 3 0 23 17 3042 0.0 3 2977 62 3 18 5 0.0 3 0 5 19 3 0.0 3 1 0 2 20 22 0.0 3 1 0 0 21 21 1 0.0 3 0 0 0 1 22 2 0.0 3 0 0 0 2 23 4 0.0 3 0 0 0 4 30 5 0.0 3 0 0 0 5 44 11 0.0 3 0 0 8 3 45 2 0.0 3 0 0 2 46 3 0.0 3 0 0 0 3 47 4 0.0 3 0 0 0 4 48 2 0.0 3 0 0 0 2 53 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 1 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 1 0 0 1 106 1 0.0 3 1 107 1 0.0 3 0 0 0 1 117 4 0.0 3 1 0 0 3 118 1 0.0 3 0 0 0 1 121 3 0.0 3 1 0 0 2 128 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 143 3 0.0 3 0 0 1 2 145 10 0.0 3 0 0 1 9 146 2 0.0 3 1 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 229 1 0.0 3 0 0 0 1 230 3 0.0 3 0 0 0 3 231 3 0.0 3 0 0 0 3 232 5 0.0 3 0 0 0 5 233 1 0.0 3 0 0 0 1 238 1 0.0 3 1 239 1 0.0 3 0 0 1 260 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 3,160 Reads with adapters: 13 (0.4%) Reads written (passing filters): 3,160 (100.0%) Total basepairs processed: 864,937 bp Total written (filtered): 864,897 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 46.2% G: 46.2% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12 49.4 0 12 4 1 12.3 0 1