This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.19 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 140,601 (5.0%) Reads that were too short: 15,079 (0.5%) Reads written (passing filters): 125,522 (4.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 30,443,546 bp (3.6%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 140601 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 185 692.0 0 185 7 8011 692.0 0 8011 8 198 692.0 0 198 9 8507 692.0 0 8507 10 36 692.0 0 36 11 33 692.0 0 33 12 58 692.0 0 58 13 43 692.0 0 43 14 55 692.0 0 55 15 36 692.0 0 36 16 54 692.0 0 54 17 60 692.0 0 60 18 50 692.0 0 50 19 35 692.0 0 35 20 56 692.0 0 56 21 47 692.0 0 47 22 56 692.0 0 56 23 40 692.0 0 40 24 56 692.0 0 56 25 61 692.0 0 61 26 86 692.0 0 86 27 93 692.0 0 93 28 174 692.0 0 174 29 276 692.0 0 276 30 102 692.0 0 102 31 142 692.0 0 142 32 332 692.0 0 332 33 108 692.0 0 108 34 561 692.0 0 561 35 156 692.0 0 156 36 118 692.0 0 118 37 125 692.0 0 125 38 258 692.0 0 258 39 158 692.0 0 158 40 159 692.0 0 159 41 234 692.0 0 234 42 3377 692.0 0 3377 43 4947 692.0 0 4947 44 4691 692.0 0 4691 45 84 692.0 0 84 46 62 692.0 0 62 47 143 692.0 0 143 48 285 692.0 0 285 49 189 692.0 0 189 50 201 692.0 0 201 51 108 692.0 0 108 52 104 692.0 0 104 53 151 692.0 0 151 54 91 692.0 0 91 55 105 692.0 0 105 56 61 692.0 0 61 57 110 692.0 0 110 58 147 692.0 0 147 59 168 692.0 0 168 60 292 692.0 0 292 61 454 692.0 0 454 62 552 692.0 0 552 63 1364 692.0 0 1364 64 2589 692.0 0 2589 65 51096 692.0 0 51096 66 9298 692.0 0 9298 67 16018 692.0 0 16018 68 103 692.0 0 103 69 67 692.0 0 67 70 40 692.0 0 40 71 45 692.0 0 45 72 45 692.0 0 45 73 35 692.0 0 35 74 27 692.0 0 27 75 66 692.0 0 66 76 70 692.0 0 70 77 57 692.0 0 57 78 52 692.0 0 52 79 47 692.0 0 47 80 44 692.0 0 44 81 53 692.0 0 53 82 45 692.0 0 45 83 44 692.0 0 44 84 42 692.0 0 42 85 56 692.0 0 56 86 35 692.0 0 35 87 55 692.0 0 55 88 75 692.0 0 75 89 111 692.0 0 111 90 161 692.0 0 161 91 36 692.0 0 36 92 47 692.0 0 47 93 56 692.0 0 56 94 66 692.0 0 66 95 70 692.0 0 70 96 67 692.0 0 67 97 46 692.0 0 46 98 56 692.0 0 56 99 63 692.0 0 63 100 84 692.0 0 84 101 63 692.0 0 63 102 145 692.0 0 145 103 52 692.0 0 52 104 47 692.0 0 47 105 34 692.0 0 34 106 44 692.0 0 44 107 49 692.0 0 49 108 43 692.0 0 43 109 34 692.0 0 34 110 51 692.0 0 51 111 60 692.0 0 60 112 84 692.0 0 84 113 48 692.0 0 48 114 52 692.0 0 52 115 36 692.0 0 36 116 36 692.0 0 36 117 47 692.0 0 47 118 52 692.0 0 52 119 65 692.0 0 65 120 52 692.0 0 52 121 58 692.0 0 58 122 40 692.0 0 40 123 56 692.0 0 56 124 54 692.0 0 54 125 35 692.0 0 35 126 53 692.0 0 53 127 50 692.0 0 50 128 41 692.0 0 41 129 59 692.0 0 59 130 61 692.0 0 61 131 69 692.0 0 69 132 47 692.0 0 47 133 76 692.0 0 76 134 168 692.0 0 168 135 119 692.0 0 119 136 254 692.0 0 254 137 102 692.0 0 102 138 344 692.0 0 344 139 51 692.0 0 51 140 44 692.0 0 44 141 57 692.0 0 57 142 40 692.0 0 40 143 55 692.0 0 55 144 60 692.0 0 60 145 33 692.0 0 33 146 46 692.0 0 46 147 107 692.0 0 107 148 49 692.0 0 49 149 39 692.0 0 39 150 38 692.0 0 38 151 34 692.0 0 34 152 39 692.0 0 39 153 39 692.0 0 39 154 56 692.0 0 56 155 29 692.0 0 29 156 41 692.0 0 41 157 70 692.0 0 70 158 52 692.0 0 52 159 87 692.0 0 87 160 61 692.0 0 61 161 46 692.0 0 46 162 60 692.0 0 60 163 33 692.0 0 33 164 35 692.0 0 35 165 39 692.0 0 39 166 38 692.0 0 38 167 36 692.0 0 36 168 53 692.0 0 53 169 43 692.0 0 43 170 42 692.0 0 42 171 33 692.0 0 33 172 51 692.0 0 51 173 57 692.0 0 57 174 64 692.0 0 64 175 41 692.0 0 41 176 33 692.0 0 33 177 32 692.0 0 32 178 92 692.0 0 92 179 83 692.0 0 83 180 63 692.0 0 63 181 95 692.0 0 95 182 53 692.0 0 53 183 79 692.0 0 79 184 114 692.0 0 114 185 59 692.0 0 59 186 57 692.0 0 57 187 29 692.0 0 29 188 38 692.0 0 38 189 54 692.0 0 54 190 55 692.0 0 55 191 40 692.0 0 40 192 42 692.0 0 42 193 35 692.0 0 35 194 46 692.0 0 46 195 31 692.0 0 31 196 55 692.0 0 55 197 67 692.0 0 67 198 78 692.0 0 78 199 49 692.0 0 49 200 60 692.0 0 60 201 93 692.0 0 93 202 123 692.0 0 123 203 197 692.0 0 197 204 81 692.0 0 81 205 51 692.0 0 51 206 39 692.0 0 39 207 49 692.0 0 49 208 49 692.0 0 49 209 69 692.0 0 69 210 56 692.0 0 56 211 67 692.0 0 67 212 66 692.0 0 66 213 61 692.0 0 61 214 63 692.0 0 63 215 65 692.0 0 65 216 85 692.0 0 85 217 140 692.0 0 140 218 79 692.0 0 79 219 130 692.0 0 130 220 77 692.0 0 77 221 86 692.0 0 86 222 338 692.0 0 338 223 302 692.0 0 302 224 564 692.0 0 564 225 290 692.0 0 290 226 45 692.0 0 45 227 47 692.0 0 47 228 44 692.0 0 44 229 45 692.0 0 45 230 50 692.0 0 50 231 59 692.0 0 59 232 44 692.0 0 44 233 51 692.0 0 51 234 40 692.0 0 40 235 70 692.0 0 70 236 58 692.0 0 58 237 70 692.0 0 70 238 67 692.0 0 67 239 56 692.0 0 56 240 61 692.0 0 61 241 70 692.0 0 70 242 292 692.0 0 292 243 311 692.0 0 311 244 382 692.0 0 382 245 96 692.0 0 96 246 79 692.0 0 79 247 81 692.0 0 81 248 52 692.0 0 52 249 70 692.0 0 70 250 73 692.0 0 73 251 121 692.0 0 121 252 133 692.0 0 133 253 176 692.0 0 176 254 190 692.0 0 190 255 1278 692.0 0 1278 256 899 692.0 0 899 257 1011 692.0 0 1011 258 234 692.0 0 234 259 127 692.0 0 127 260 388 692.0 0 388 261 826 692.0 0 826 262 404 692.0 0 404 263 104 692.0 0 104 264 58 692.0 0 58 265 93 692.0 0 93 266 129 692.0 0 129 267 102 692.0 0 102 268 43 692.0 0 43 269 66 692.0 0 66 270 386 692.0 0 386 271 200 692.0 0 200 272 350 692.0 0 350 273 63 692.0 0 63 274 94 692.0 0 94 275 61 692.0 0 61 276 96 692.0 0 96 277 116 692.0 0 116 278 135 692.0 0 135 279 156 692.0 0 156 280 69 692.0 0 69 281 38 692.0 0 38 282 48 692.0 0 48 283 66 692.0 0 66 284 59 692.0 0 59 285 50 692.0 0 50 286 53 692.0 0 53 287 42 692.0 0 42 288 42 692.0 0 42 289 46 692.0 0 46 290 80 692.0 0 80 291 95 692.0 0 95 292 114 692.0 0 114 293 93 692.0 0 93 294 98 692.0 0 98 295 47 692.0 0 47 296 68 692.0 0 68 297 77 692.0 0 77 298 78 692.0 0 78 299 116 692.0 0 116 300 142 692.0 0 142 301 79 692.0 0 79 Finished in 5.74 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 125,522 Reads with adapters: 8,157 (6.5%) Reads that were too short: 92 (0.1%) Reads written (passing filters): 8,065 (6.4%) Total basepairs processed: 30,443,546 bp Total written (filtered): 2,199,342 bp (7.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8157 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 2 0 1 19 105 0.0 3 0 93 5 6 1 20 7835 0.0 4 7499 276 43 13 4 21 19 0.0 4 4 13 1 1 22 2 0.0 4 0 0 1 1 23 4 0.0 4 1 0 0 3 24 2 0.0 4 0 0 0 0 2 56 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 0 2 92 5 0.0 4 0 0 0 0 5 114 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 122 1 0.0 4 1 124 3 0.0 4 1 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 73 0.0 4 0 0 0 0 73 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 175 2 0.0 4 0 0 0 0 2 178 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 192 2 0.0 4 0 0 0 0 2 193 36 0.0 4 0 0 0 1 35 194 20 0.0 4 0 0 0 0 20 195 3 0.0 4 0 0 0 0 3 203 3 0.0 4 0 0 0 1 2 206 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 7 0.0 4 0 0 0 1 6 230 3 0.0 4 0 0 0 0 3 244 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 8,065 Reads with adapters: 430 (5.3%) Reads written (passing filters): 8,065 (100.0%) Total basepairs processed: 2,199,342 bp Total written (filtered): 2,161,867 bp (98.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 430 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.2% C: 11.6% G: 44.7% T: 36.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 41 126.0 0 41 4 3 31.5 0 3 9 3 0.0 0 1 2 10 10 0.0 1 1 9 11 78 0.0 1 7 71 12 135 0.0 1 5 130 13 3 0.0 1 0 3 14 2 0.0 1 0 2 15 1 0.0 1 0 1 225 151 0.0 1 132 19 226 3 0.0 1 3