This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.86 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 66,128 (2.3%) Reads that were too short: 24,461 (0.9%) Reads written (passing filters): 41,667 (1.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 8,552,870 bp (1.0%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 66128 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1636 692.0 0 1636 7 185 692.0 0 185 8 5707 692.0 0 5707 9 5210 692.0 0 5210 10 45 692.0 0 45 11 31 692.0 0 31 12 44 692.0 0 44 13 36 692.0 0 36 14 38 692.0 0 38 15 38 692.0 0 38 16 44 692.0 0 44 17 57 692.0 0 57 18 54 692.0 0 54 19 52 692.0 0 52 20 40 692.0 0 40 21 38 692.0 0 38 22 64 692.0 0 64 23 66 692.0 0 66 24 82 692.0 0 82 25 93 692.0 0 93 26 70 692.0 0 70 27 56 692.0 0 56 28 45 692.0 0 45 29 92 692.0 0 92 30 49 692.0 0 49 31 69 692.0 0 69 32 39 692.0 0 39 33 37 692.0 0 37 34 57 692.0 0 57 35 36 692.0 0 36 36 101 692.0 0 101 37 36 692.0 0 36 38 50 692.0 0 50 39 35 692.0 0 35 40 32 692.0 0 32 41 36 692.0 0 36 42 45 692.0 0 45 43 47 692.0 0 47 44 30 692.0 0 30 45 38 692.0 0 38 46 46 692.0 0 46 47 44 692.0 0 44 48 39 692.0 0 39 49 45 692.0 0 45 50 47 692.0 0 47 51 40 692.0 0 40 52 49 692.0 0 49 53 43 692.0 0 43 54 40 692.0 0 40 55 39 692.0 0 39 56 45 692.0 0 45 57 58 692.0 0 58 58 59 692.0 0 59 59 36 692.0 0 36 60 47 692.0 0 47 61 33 692.0 0 33 62 50 692.0 0 50 63 47 692.0 0 47 64 42 692.0 0 42 65 76 692.0 0 76 66 40 692.0 0 40 67 81 692.0 0 81 68 49 692.0 0 49 69 413 692.0 0 413 70 58 692.0 0 58 71 189 692.0 0 189 72 53 692.0 0 53 73 49 692.0 0 49 74 38 692.0 0 38 75 77 692.0 0 77 76 101 692.0 0 101 77 116 692.0 0 116 78 224 692.0 0 224 79 98 692.0 0 98 80 297 692.0 0 297 81 120 692.0 0 120 82 298 692.0 0 298 83 102 692.0 0 102 84 67 692.0 0 67 85 76 692.0 0 76 86 93 692.0 0 93 87 88 692.0 0 88 88 53 692.0 0 53 89 71 692.0 0 71 90 98 692.0 0 98 91 52 692.0 0 52 92 63 692.0 0 63 93 54 692.0 0 54 94 106 692.0 0 106 95 127 692.0 0 127 96 1285 692.0 0 1285 97 726 692.0 0 726 98 1586 692.0 0 1586 99 80 692.0 0 80 100 55 692.0 0 55 101 61 692.0 0 61 102 42 692.0 0 42 103 57 692.0 0 57 104 46 692.0 0 46 105 52 692.0 0 52 106 45 692.0 0 45 107 47 692.0 0 47 108 69 692.0 0 69 109 41 692.0 0 41 110 52 692.0 0 52 111 61 692.0 0 61 112 72 692.0 0 72 113 91 692.0 0 91 114 99 692.0 0 99 115 58 692.0 0 58 116 51 692.0 0 51 117 61 692.0 0 61 118 52 692.0 0 52 119 55 692.0 0 55 120 92 692.0 0 92 121 50 692.0 0 50 122 71 692.0 0 71 123 48 692.0 0 48 124 74 692.0 0 74 125 53 692.0 0 53 126 270 692.0 0 270 127 199 692.0 0 199 128 515 692.0 0 515 129 160 692.0 0 160 130 238 692.0 0 238 131 138 692.0 0 138 132 481 692.0 0 481 133 419 692.0 0 419 134 991 692.0 0 991 135 162 692.0 0 162 136 84 692.0 0 84 137 55 692.0 0 55 138 66 692.0 0 66 139 109 692.0 0 109 140 69 692.0 0 69 141 74 692.0 0 74 142 49 692.0 0 49 143 52 692.0 0 52 144 64 692.0 0 64 145 52 692.0 0 52 146 54 692.0 0 54 147 68 692.0 0 68 148 346 692.0 0 346 149 535 692.0 0 535 150 333 692.0 0 333 151 50 692.0 0 50 152 46 692.0 0 46 153 71 692.0 0 71 154 30 692.0 0 30 155 55 692.0 0 55 156 54 692.0 0 54 157 66 692.0 0 66 158 75 692.0 0 75 159 67 692.0 0 67 160 65 692.0 0 65 161 69 692.0 0 69 162 61 692.0 0 61 163 47 692.0 0 47 164 58 692.0 0 58 165 54 692.0 0 54 166 64 692.0 0 64 167 56 692.0 0 56 168 34 692.0 0 34 169 61 692.0 0 61 170 50 692.0 0 50 171 62 692.0 0 62 172 80 692.0 0 80 173 99 692.0 0 99 174 145 692.0 0 145 175 159 692.0 0 159 176 71 692.0 0 71 177 88 692.0 0 88 178 98 692.0 0 98 179 87 692.0 0 87 180 70 692.0 0 70 181 100 692.0 0 100 182 87 692.0 0 87 183 77 692.0 0 77 184 125 692.0 0 125 185 210 692.0 0 210 186 261 692.0 0 261 187 1276 692.0 0 1276 188 2402 692.0 0 2402 189 3765 692.0 0 3765 190 959 692.0 0 959 191 61 692.0 0 61 192 68 692.0 0 68 193 47 692.0 0 47 194 62 692.0 0 62 195 49 692.0 0 49 196 69 692.0 0 69 197 64 692.0 0 64 198 65 692.0 0 65 199 60 692.0 0 60 200 56 692.0 0 56 201 61 692.0 0 61 202 66 692.0 0 66 203 74 692.0 0 74 204 56 692.0 0 56 205 86 692.0 0 86 206 88 692.0 0 88 207 130 692.0 0 130 208 111 692.0 0 111 209 760 692.0 0 760 210 701 692.0 0 701 211 2092 692.0 0 2092 212 148 692.0 0 148 213 67 692.0 0 67 214 72 692.0 0 72 215 63 692.0 0 63 216 45 692.0 0 45 217 58 692.0 0 58 218 57 692.0 0 57 219 58 692.0 0 58 220 69 692.0 0 69 221 55 692.0 0 55 222 66 692.0 0 66 223 80 692.0 0 80 224 83 692.0 0 83 225 118 692.0 0 118 226 263 692.0 0 263 227 109 692.0 0 109 228 169 692.0 0 169 229 62 692.0 0 62 230 165 692.0 0 165 231 100 692.0 0 100 232 116 692.0 0 116 233 195 692.0 0 195 234 202 692.0 0 202 235 249 692.0 0 249 236 205 692.0 0 205 237 110 692.0 0 110 238 95 692.0 0 95 239 109 692.0 0 109 240 480 692.0 0 480 241 352 692.0 0 352 242 762 692.0 0 762 243 264 692.0 0 264 244 177 692.0 0 177 245 124 692.0 0 124 246 181 692.0 0 181 247 296 692.0 0 296 248 851 692.0 0 851 249 449 692.0 0 449 250 210 692.0 0 210 251 388 692.0 0 388 252 202 692.0 0 202 253 461 692.0 0 461 254 867 692.0 0 867 255 1058 692.0 0 1058 256 209 692.0 0 209 257 111 692.0 0 111 258 251 692.0 0 251 259 137 692.0 0 137 260 387 692.0 0 387 261 309 692.0 0 309 262 502 692.0 0 502 263 156 692.0 0 156 264 89 692.0 0 89 265 204 692.0 0 204 266 144 692.0 0 144 267 352 692.0 0 352 268 179 692.0 0 179 269 560 692.0 0 560 270 389 692.0 0 389 271 1041 692.0 0 1041 272 715 692.0 0 715 273 477 692.0 0 477 274 1338 692.0 0 1338 275 182 692.0 0 182 276 66 692.0 0 66 277 66 692.0 0 66 278 67 692.0 0 67 279 96 692.0 0 96 280 82 692.0 0 82 281 103 692.0 0 103 282 72 692.0 0 72 283 221 692.0 0 221 284 104 692.0 0 104 285 90 692.0 0 90 286 52 692.0 0 52 287 51 692.0 0 51 288 63 692.0 0 63 289 70 692.0 0 70 290 49 692.0 0 49 291 60 692.0 0 60 292 69 692.0 0 69 293 71 692.0 0 71 294 94 692.0 0 94 295 92 692.0 0 92 296 97 692.0 0 97 297 55 692.0 0 55 298 161 692.0 0 161 299 93 692.0 0 93 300 90 692.0 0 90 301 121 692.0 0 121 Finished in 1.82 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 41,667 Reads with adapters: 5,438 (13.1%) Reads that were too short: 86 (0.2%) Reads written (passing filters): 5,352 (12.8%) Total basepairs processed: 8,552,870 bp Total written (filtered): 1,453,739 bp (17.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5438 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 8 0.0 3 0 0 6 1 1 19 102 0.0 3 0 87 7 6 2 20 5187 0.0 4 4817 301 49 17 3 21 6 0.0 4 0 6 23 27 0.0 4 0 0 0 23 4 25 1 0.0 4 0 0 0 0 1 28 4 0.0 4 0 0 0 0 4 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 1 41 6 0.0 4 0 0 0 0 6 42 3 0.0 4 0 0 0 0 3 43 2 0.0 4 0 0 0 0 2 54 10 0.0 4 0 0 0 1 9 55 4 0.0 4 0 0 0 2 2 56 28 0.0 4 0 0 0 2 26 58 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 103 3 0.0 4 0 0 0 0 3 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 3 0.0 4 0 0 0 0 3 116 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 130 5 0.0 4 0 0 0 0 5 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 2 0.0 4 1 1 147 3 0.0 4 0 0 0 0 3 149 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 259 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 5,352 Reads with adapters: 1,354 (25.3%) Reads written (passing filters): 5,352 (100.0%) Total basepairs processed: 1,453,739 bp Total written (filtered): 1,437,754 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1354 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 9.6% G: 84.9% T: 3.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 184 0.0 0 18 166 10 1087 0.0 1 100 987 11 62 0.0 1 3 59 12 1 0.0 1 0 1 13 6 0.0 1 1 5 72 1 0.0 1 1 73 1 0.0 1 1 138 1 0.0 1 1 150 1 0.0 1 1 208 1 0.0 1 1 223 8 0.0 1 7 1 262 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.