This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.58 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 34,222 (1.2%) Reads that were too short: 13,566 (0.5%) Reads written (passing filters): 20,656 (0.7%) Total basepairs processed: 853,219,115 bp Total written (filtered): 4,101,071 bp (0.5%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 34222 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 102 692.0 0 102 7 1104 692.0 0 1104 8 953 692.0 0 953 9 87 692.0 0 87 10 131 692.0 0 131 11 29 692.0 0 29 12 27 692.0 0 27 13 25 692.0 0 25 14 29 692.0 0 29 15 17 692.0 0 17 16 30 692.0 0 30 17 20 692.0 0 20 18 19 692.0 0 19 19 26 692.0 0 26 20 23 692.0 0 23 21 31 692.0 0 31 22 58 692.0 0 58 23 23 692.0 0 23 24 21 692.0 0 21 25 26 692.0 0 26 26 15 692.0 0 15 27 12 692.0 0 12 28 21 692.0 0 21 29 28 692.0 0 28 30 27 692.0 0 27 31 30 692.0 0 30 32 27 692.0 0 27 33 16 692.0 0 16 34 24 692.0 0 24 35 26 692.0 0 26 36 33 692.0 0 33 37 29 692.0 0 29 38 45 692.0 0 45 39 31 692.0 0 31 40 60 692.0 0 60 41 31 692.0 0 31 42 25 692.0 0 25 43 31 692.0 0 31 44 26 692.0 0 26 45 32 692.0 0 32 46 34 692.0 0 34 47 30 692.0 0 30 48 476 692.0 0 476 49 86 692.0 0 86 50 29 692.0 0 29 51 26 692.0 0 26 52 27 692.0 0 27 53 32 692.0 0 32 54 22 692.0 0 22 55 26 692.0 0 26 56 26 692.0 0 26 57 26 692.0 0 26 58 22 692.0 0 22 59 35 692.0 0 35 60 81 692.0 0 81 61 37 692.0 0 37 62 35 692.0 0 35 63 28 692.0 0 28 64 28 692.0 0 28 65 39 692.0 0 39 66 29 692.0 0 29 67 30 692.0 0 30 68 46 692.0 0 46 69 525 692.0 0 525 70 160 692.0 0 160 71 379 692.0 0 379 72 216 692.0 0 216 73 467 692.0 0 467 74 218 692.0 0 218 75 67 692.0 0 67 76 24 692.0 0 24 77 33 692.0 0 33 78 39 692.0 0 39 79 106 692.0 0 106 80 114 692.0 0 114 81 357 692.0 0 357 82 133 692.0 0 133 83 301 692.0 0 301 84 702 692.0 0 702 85 1886 692.0 0 1886 86 1721 692.0 0 1721 87 30 692.0 0 30 88 67 692.0 0 67 89 39 692.0 0 39 90 56 692.0 0 56 91 52 692.0 0 52 92 25 692.0 0 25 93 26 692.0 0 26 94 38 692.0 0 38 95 31 692.0 0 31 96 29 692.0 0 29 97 29 692.0 0 29 98 31 692.0 0 31 99 54 692.0 0 54 100 34 692.0 0 34 101 38 692.0 0 38 102 31 692.0 0 31 103 25 692.0 0 25 104 20 692.0 0 20 105 13 692.0 0 13 106 16 692.0 0 16 107 30 692.0 0 30 108 22 692.0 0 22 109 26 692.0 0 26 110 49 692.0 0 49 111 92 692.0 0 92 112 110 692.0 0 110 113 36 692.0 0 36 114 25 692.0 0 25 115 27 692.0 0 27 116 15 692.0 0 15 117 20 692.0 0 20 118 20 692.0 0 20 119 38 692.0 0 38 120 23 692.0 0 23 121 47 692.0 0 47 122 24 692.0 0 24 123 23 692.0 0 23 124 31 692.0 0 31 125 32 692.0 0 32 126 19 692.0 0 19 127 24 692.0 0 24 128 25 692.0 0 25 129 48 692.0 0 48 130 77 692.0 0 77 131 52 692.0 0 52 132 51 692.0 0 51 133 36 692.0 0 36 134 27 692.0 0 27 135 31 692.0 0 31 136 29 692.0 0 29 137 47 692.0 0 47 138 60 692.0 0 60 139 36 692.0 0 36 140 28 692.0 0 28 141 39 692.0 0 39 142 68 692.0 0 68 143 44 692.0 0 44 144 38 692.0 0 38 145 91 692.0 0 91 146 72 692.0 0 72 147 71 692.0 0 71 148 163 692.0 0 163 149 91 692.0 0 91 150 97 692.0 0 97 151 82 692.0 0 82 152 80 692.0 0 80 153 54 692.0 0 54 154 22 692.0 0 22 155 32 692.0 0 32 156 60 692.0 0 60 157 86 692.0 0 86 158 132 692.0 0 132 159 72 692.0 0 72 160 32 692.0 0 32 161 41 692.0 0 41 162 35 692.0 0 35 163 27 692.0 0 27 164 23 692.0 0 23 165 34 692.0 0 34 166 42 692.0 0 42 167 45 692.0 0 45 168 96 692.0 0 96 169 88 692.0 0 88 170 123 692.0 0 123 171 49 692.0 0 49 172 38 692.0 0 38 173 43 692.0 0 43 174 65 692.0 0 65 175 123 692.0 0 123 176 914 692.0 0 914 177 296 692.0 0 296 178 451 692.0 0 451 179 33 692.0 0 33 180 35 692.0 0 35 181 31 692.0 0 31 182 34 692.0 0 34 183 39 692.0 0 39 184 35 692.0 0 35 185 42 692.0 0 42 186 39 692.0 0 39 187 86 692.0 0 86 188 164 692.0 0 164 189 139 692.0 0 139 190 58 692.0 0 58 191 42 692.0 0 42 192 29 692.0 0 29 193 43 692.0 0 43 194 63 692.0 0 63 195 104 692.0 0 104 196 206 692.0 0 206 197 269 692.0 0 269 198 489 692.0 0 489 199 564 692.0 0 564 200 82 692.0 0 82 201 59 692.0 0 59 202 573 692.0 0 573 203 344 692.0 0 344 204 575 692.0 0 575 205 450 692.0 0 450 206 188 692.0 0 188 207 51 692.0 0 51 208 46 692.0 0 46 209 90 692.0 0 90 210 134 692.0 0 134 211 108 692.0 0 108 212 111 692.0 0 111 213 215 692.0 0 215 214 267 692.0 0 267 215 393 692.0 0 393 216 71 692.0 0 71 217 90 692.0 0 90 218 105 692.0 0 105 219 54 692.0 0 54 220 54 692.0 0 54 221 40 692.0 0 40 222 156 692.0 0 156 223 670 692.0 0 670 224 884 692.0 0 884 225 534 692.0 0 534 226 48 692.0 0 48 227 40 692.0 0 40 228 47 692.0 0 47 229 30 692.0 0 30 230 39 692.0 0 39 231 27 692.0 0 27 232 21 692.0 0 21 233 59 692.0 0 59 234 50 692.0 0 50 235 59 692.0 0 59 236 51 692.0 0 51 237 59 692.0 0 59 238 87 692.0 0 87 239 81 692.0 0 81 240 57 692.0 0 57 241 123 692.0 0 123 242 78 692.0 0 78 243 95 692.0 0 95 244 156 692.0 0 156 245 109 692.0 0 109 246 98 692.0 0 98 247 65 692.0 0 65 248 60 692.0 0 60 249 56 692.0 0 56 250 66 692.0 0 66 251 43 692.0 0 43 252 41 692.0 0 41 253 55 692.0 0 55 254 65 692.0 0 65 255 152 692.0 0 152 256 36 692.0 0 36 257 63 692.0 0 63 258 287 692.0 0 287 259 130 692.0 0 130 260 211 692.0 0 211 261 94 692.0 0 94 262 59 692.0 0 59 263 53 692.0 0 53 264 52 692.0 0 52 265 37 692.0 0 37 266 35 692.0 0 35 267 33 692.0 0 33 268 35 692.0 0 35 269 50 692.0 0 50 270 117 692.0 0 117 271 495 692.0 0 495 272 160 692.0 0 160 273 155 692.0 0 155 274 44 692.0 0 44 275 54 692.0 0 54 276 49 692.0 0 49 277 59 692.0 0 59 278 59 692.0 0 59 279 104 692.0 0 104 280 51 692.0 0 51 281 61 692.0 0 61 282 148 692.0 0 148 283 133 692.0 0 133 284 226 692.0 0 226 285 70 692.0 0 70 286 86 692.0 0 86 287 93 692.0 0 93 288 55 692.0 0 55 289 48 692.0 0 48 290 51 692.0 0 51 291 73 692.0 0 73 292 78 692.0 0 78 293 54 692.0 0 54 294 71 692.0 0 71 295 68 692.0 0 68 296 120 692.0 0 120 297 295 692.0 0 295 298 421 692.0 0 421 299 406 692.0 0 406 300 165 692.0 0 165 301 102 692.0 0 102 Finished in 0.86 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 20,656 Reads with adapters: 1,002 (4.9%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 996 (4.8%) Total basepairs processed: 4,101,071 bp Total written (filtered): 271,407 bp (6.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1002 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 23 0.0 3 0 18 2 3 20 945 0.0 4 883 52 3 6 1 21 4 0.0 4 3 1 22 1 0.0 4 0 0 0 0 1 23 17 0.0 4 10 2 1 4 32 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 1 69 1 0.0 4 0 0 0 1 75 2 0.0 4 1 0 0 0 1 85 1 0.0 4 0 0 0 0 1 96 1 0.0 4 1 119 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 996 Reads with adapters: 182 (18.3%) Reads written (passing filters): 996 (100.0%) Total basepairs processed: 271,407 bp Total written (filtered): 269,439 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 182 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 10.4% G: 85.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 2 0.0 0 0 2 10 31 0.0 1 7 24 11 148 0.0 1 6 142 12 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.