This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.66 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 18,569 (0.7%) Reads that were too short: 5,778 (0.2%) Reads written (passing filters): 12,791 (0.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 2,727,834 bp (0.3%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 18569 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 92 692.0 0 92 7 1014 692.0 0 1014 8 1166 692.0 0 1166 9 45 692.0 0 45 10 23 692.0 0 23 11 34 692.0 0 34 12 35 692.0 0 35 13 19 692.0 0 19 14 41 692.0 0 41 15 21 692.0 0 21 16 19 692.0 0 19 17 21 692.0 0 21 18 22 692.0 0 22 19 30 692.0 0 30 20 29 692.0 0 29 21 21 692.0 0 21 22 31 692.0 0 31 23 25 692.0 0 25 24 16 692.0 0 16 25 24 692.0 0 24 26 15 692.0 0 15 27 20 692.0 0 20 28 26 692.0 0 26 29 22 692.0 0 22 30 28 692.0 0 28 31 29 692.0 0 29 32 15 692.0 0 15 33 16 692.0 0 16 34 19 692.0 0 19 35 20 692.0 0 20 36 26 692.0 0 26 37 36 692.0 0 36 38 28 692.0 0 28 39 34 692.0 0 34 40 27 692.0 0 27 41 31 692.0 0 31 42 18 692.0 0 18 43 24 692.0 0 24 44 23 692.0 0 23 45 34 692.0 0 34 46 27 692.0 0 27 47 27 692.0 0 27 48 29 692.0 0 29 49 34 692.0 0 34 50 47 692.0 0 47 51 24 692.0 0 24 52 41 692.0 0 41 53 24 692.0 0 24 54 32 692.0 0 32 55 46 692.0 0 46 56 31 692.0 0 31 57 28 692.0 0 28 58 30 692.0 0 30 59 24 692.0 0 24 60 33 692.0 0 33 61 41 692.0 0 41 62 29 692.0 0 29 63 17 692.0 0 17 64 24 692.0 0 24 65 33 692.0 0 33 66 31 692.0 0 31 67 35 692.0 0 35 68 46 692.0 0 46 69 370 692.0 0 370 70 113 692.0 0 113 71 435 692.0 0 435 72 196 692.0 0 196 73 486 692.0 0 486 74 237 692.0 0 237 75 68 692.0 0 68 76 27 692.0 0 27 77 41 692.0 0 41 78 29 692.0 0 29 79 104 692.0 0 104 80 89 692.0 0 89 81 384 692.0 0 384 82 129 692.0 0 129 83 328 692.0 0 328 84 795 692.0 0 795 85 201 692.0 0 201 86 167 692.0 0 167 87 25 692.0 0 25 88 23 692.0 0 23 89 26 692.0 0 26 90 21 692.0 0 21 91 26 692.0 0 26 92 25 692.0 0 25 93 26 692.0 0 26 94 39 692.0 0 39 95 34 692.0 0 34 96 24 692.0 0 24 97 25 692.0 0 25 98 23 692.0 0 23 99 46 692.0 0 46 100 30 692.0 0 30 101 19 692.0 0 19 102 32 692.0 0 32 103 24 692.0 0 24 104 25 692.0 0 25 105 28 692.0 0 28 106 22 692.0 0 22 107 29 692.0 0 29 108 22 692.0 0 22 109 18 692.0 0 18 110 29 692.0 0 29 111 97 692.0 0 97 112 88 692.0 0 88 113 21 692.0 0 21 114 20 692.0 0 20 115 14 692.0 0 14 116 28 692.0 0 28 117 25 692.0 0 25 118 24 692.0 0 24 119 41 692.0 0 41 120 30 692.0 0 30 121 36 692.0 0 36 122 24 692.0 0 24 123 18 692.0 0 18 124 33 692.0 0 33 125 31 692.0 0 31 126 20 692.0 0 20 127 21 692.0 0 21 128 21 692.0 0 21 129 33 692.0 0 33 130 53 692.0 0 53 131 25 692.0 0 25 132 27 692.0 0 27 133 36 692.0 0 36 134 36 692.0 0 36 135 33 692.0 0 33 136 25 692.0 0 25 137 28 692.0 0 28 138 19 692.0 0 19 139 31 692.0 0 31 140 20 692.0 0 20 141 22 692.0 0 22 142 36 692.0 0 36 143 23 692.0 0 23 144 51 692.0 0 51 145 25 692.0 0 25 146 24 692.0 0 24 147 61 692.0 0 61 148 163 692.0 0 163 149 66 692.0 0 66 150 37 692.0 0 37 151 28 692.0 0 28 152 27 692.0 0 27 153 31 692.0 0 31 154 26 692.0 0 26 155 27 692.0 0 27 156 25 692.0 0 25 157 33 692.0 0 33 158 81 692.0 0 81 159 44 692.0 0 44 160 26 692.0 0 26 161 29 692.0 0 29 162 19 692.0 0 19 163 28 692.0 0 28 164 19 692.0 0 19 165 27 692.0 0 27 166 30 692.0 0 30 167 29 692.0 0 29 168 71 692.0 0 71 169 70 692.0 0 70 170 59 692.0 0 59 171 30 692.0 0 30 172 20 692.0 0 20 173 19 692.0 0 19 174 23 692.0 0 23 175 44 692.0 0 44 176 228 692.0 0 228 177 106 692.0 0 106 178 139 692.0 0 139 179 31 692.0 0 31 180 28 692.0 0 28 181 18 692.0 0 18 182 45 692.0 0 45 183 24 692.0 0 24 184 29 692.0 0 29 185 31 692.0 0 31 186 24 692.0 0 24 187 48 692.0 0 48 188 60 692.0 0 60 189 53 692.0 0 53 190 37 692.0 0 37 191 33 692.0 0 33 192 37 692.0 0 37 193 30 692.0 0 30 194 25 692.0 0 25 195 30 692.0 0 30 196 65 692.0 0 65 197 91 692.0 0 91 198 158 692.0 0 158 199 222 692.0 0 222 200 50 692.0 0 50 201 26 692.0 0 26 202 138 692.0 0 138 203 99 692.0 0 99 204 187 692.0 0 187 205 120 692.0 0 120 206 44 692.0 0 44 207 45 692.0 0 45 208 24 692.0 0 24 209 31 692.0 0 31 210 59 692.0 0 59 211 55 692.0 0 55 212 32 692.0 0 32 213 57 692.0 0 57 214 88 692.0 0 88 215 98 692.0 0 98 216 27 692.0 0 27 217 47 692.0 0 47 218 45 692.0 0 45 219 27 692.0 0 27 220 27 692.0 0 27 221 35 692.0 0 35 222 44 692.0 0 44 223 166 692.0 0 166 224 310 692.0 0 310 225 226 692.0 0 226 226 32 692.0 0 32 227 26 692.0 0 26 228 42 692.0 0 42 229 28 692.0 0 28 230 28 692.0 0 28 231 18 692.0 0 18 232 21 692.0 0 21 233 33 692.0 0 33 234 14 692.0 0 14 235 25 692.0 0 25 236 27 692.0 0 27 237 37 692.0 0 37 238 43 692.0 0 43 239 33 692.0 0 33 240 51 692.0 0 51 241 40 692.0 0 40 242 41 692.0 0 41 243 43 692.0 0 43 244 62 692.0 0 62 245 38 692.0 0 38 246 37 692.0 0 37 247 45 692.0 0 45 248 59 692.0 0 59 249 40 692.0 0 40 250 44 692.0 0 44 251 30 692.0 0 30 252 33 692.0 0 33 253 32 692.0 0 32 254 38 692.0 0 38 255 61 692.0 0 61 256 28 692.0 0 28 257 35 692.0 0 35 258 153 692.0 0 153 259 90 692.0 0 90 260 165 692.0 0 165 261 65 692.0 0 65 262 29 692.0 0 29 263 25 692.0 0 25 264 25 692.0 0 25 265 22 692.0 0 22 266 22 692.0 0 22 267 20 692.0 0 20 268 19 692.0 0 19 269 20 692.0 0 20 270 87 692.0 0 87 271 219 692.0 0 219 272 91 692.0 0 91 273 52 692.0 0 52 274 28 692.0 0 28 275 24 692.0 0 24 276 20 692.0 0 20 277 23 692.0 0 23 278 36 692.0 0 36 279 44 692.0 0 44 280 28 692.0 0 28 281 38 692.0 0 38 282 45 692.0 0 45 283 38 692.0 0 38 284 139 692.0 0 139 285 43 692.0 0 43 286 45 692.0 0 45 287 54 692.0 0 54 288 36 692.0 0 36 289 26 692.0 0 26 290 23 692.0 0 23 291 30 692.0 0 30 292 25 692.0 0 25 293 37 692.0 0 37 294 46 692.0 0 46 295 46 692.0 0 46 296 37 692.0 0 37 297 128 692.0 0 128 298 145 692.0 0 145 299 172 692.0 0 172 300 64 692.0 0 64 301 59 692.0 0 59 Finished in 0.52 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 12,791 Reads with adapters: 991 (7.7%) Reads that were too short: 16 (0.1%) Reads written (passing filters): 975 (7.6%) Total basepairs processed: 2,727,834 bp Total written (filtered): 267,909 bp (9.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 991 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 3 0.0 3 0 2 0 1 17 948 0.0 3 915 31 2 18 2 0.0 3 0 2 19 3 0.0 3 3 20 4 0.0 3 1 2 0 1 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 44 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 0 1 73 1 0.0 3 1 81 2 0.0 3 0 0 1 1 89 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 1 112 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 229 1 0.0 3 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 975 Reads with adapters: 4 (0.4%) Reads written (passing filters): 975 (100.0%) Total basepairs processed: 267,909 bp Total written (filtered): 267,896 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 25.0% G: 0.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 15.2 0 3 4 1 3.8 0 1