This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.01 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 97,463 (3.4%) Reads that were too short: 23,592 (0.8%) Reads written (passing filters): 73,871 (2.6%) Total basepairs processed: 853,219,115 bp Total written (filtered): 16,606,637 bp (1.9%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 97463 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5533 692.0 0 5533 7 17084 692.0 0 17084 8 1076 692.0 0 1076 9 14585 692.0 0 14585 10 56 692.0 0 56 11 115 692.0 0 115 12 27 692.0 0 27 13 39 692.0 0 39 14 27 692.0 0 27 15 38 692.0 0 38 16 23 692.0 0 23 17 23 692.0 0 23 18 35 692.0 0 35 19 31 692.0 0 31 20 22 692.0 0 22 21 27 692.0 0 27 22 25 692.0 0 25 23 25 692.0 0 25 24 16 692.0 0 16 25 18 692.0 0 18 26 22 692.0 0 22 27 30 692.0 0 30 28 37 692.0 0 37 29 32 692.0 0 32 30 49 692.0 0 49 31 25 692.0 0 25 32 18 692.0 0 18 33 20 692.0 0 20 34 28 692.0 0 28 35 38 692.0 0 38 36 45 692.0 0 45 37 42 692.0 0 42 38 32 692.0 0 32 39 30 692.0 0 30 40 121 692.0 0 121 41 28 692.0 0 28 42 64 692.0 0 64 43 24 692.0 0 24 44 33 692.0 0 33 45 33 692.0 0 33 46 28 692.0 0 28 47 32 692.0 0 32 48 31 692.0 0 31 49 47 692.0 0 47 50 38 692.0 0 38 51 21 692.0 0 21 52 27 692.0 0 27 53 23 692.0 0 23 54 27 692.0 0 27 55 176 692.0 0 176 56 75 692.0 0 75 57 124 692.0 0 124 58 44 692.0 0 44 59 58 692.0 0 58 60 47 692.0 0 47 61 42 692.0 0 42 62 32 692.0 0 32 63 29 692.0 0 29 64 41 692.0 0 41 65 40 692.0 0 40 66 68 692.0 0 68 67 44 692.0 0 44 68 57 692.0 0 57 69 38 692.0 0 38 70 34 692.0 0 34 71 61 692.0 0 61 72 34 692.0 0 34 73 54 692.0 0 54 74 26 692.0 0 26 75 76 692.0 0 76 76 90 692.0 0 90 77 38 692.0 0 38 78 17 692.0 0 17 79 47 692.0 0 47 80 20 692.0 0 20 81 34 692.0 0 34 82 37 692.0 0 37 83 50 692.0 0 50 84 61 692.0 0 61 85 33 692.0 0 33 86 38 692.0 0 38 87 54 692.0 0 54 88 43 692.0 0 43 89 66 692.0 0 66 90 128 692.0 0 128 91 52 692.0 0 52 92 72 692.0 0 72 93 29 692.0 0 29 94 53 692.0 0 53 95 47 692.0 0 47 96 54 692.0 0 54 97 42 692.0 0 42 98 29 692.0 0 29 99 14 692.0 0 14 100 24 692.0 0 24 101 29 692.0 0 29 102 36 692.0 0 36 103 61 692.0 0 61 104 57 692.0 0 57 105 259 692.0 0 259 106 228 692.0 0 228 107 208 692.0 0 208 108 51 692.0 0 51 109 34 692.0 0 34 110 40 692.0 0 40 111 40 692.0 0 40 112 40 692.0 0 40 113 59 692.0 0 59 114 69 692.0 0 69 115 98 692.0 0 98 116 188 692.0 0 188 117 66 692.0 0 66 118 50 692.0 0 50 119 39 692.0 0 39 120 38 692.0 0 38 121 33 692.0 0 33 122 30 692.0 0 30 123 38 692.0 0 38 124 34 692.0 0 34 125 46 692.0 0 46 126 98 692.0 0 98 127 66 692.0 0 66 128 63 692.0 0 63 129 70 692.0 0 70 130 183 692.0 0 183 131 79 692.0 0 79 132 117 692.0 0 117 133 119 692.0 0 119 134 171 692.0 0 171 135 62 692.0 0 62 136 80 692.0 0 80 137 139 692.0 0 139 138 77 692.0 0 77 139 119 692.0 0 119 140 107 692.0 0 107 141 106 692.0 0 106 142 142 692.0 0 142 143 128 692.0 0 128 144 193 692.0 0 193 145 172 692.0 0 172 146 195 692.0 0 195 147 2345 692.0 0 2345 148 1848 692.0 0 1848 149 9763 692.0 0 9763 150 224 692.0 0 224 151 124 692.0 0 124 152 283 692.0 0 283 153 98 692.0 0 98 154 367 692.0 0 367 155 147 692.0 0 147 156 100 692.0 0 100 157 54 692.0 0 54 158 50 692.0 0 50 159 136 692.0 0 136 160 55 692.0 0 55 161 48 692.0 0 48 162 58 692.0 0 58 163 39 692.0 0 39 164 46 692.0 0 46 165 32 692.0 0 32 166 41 692.0 0 41 167 51 692.0 0 51 168 90 692.0 0 90 169 86 692.0 0 86 170 148 692.0 0 148 171 102 692.0 0 102 172 106 692.0 0 106 173 98 692.0 0 98 174 179 692.0 0 179 175 144 692.0 0 144 176 66 692.0 0 66 177 89 692.0 0 89 178 86 692.0 0 86 179 59 692.0 0 59 180 55 692.0 0 55 181 43 692.0 0 43 182 81 692.0 0 81 183 93 692.0 0 93 184 158 692.0 0 158 185 64 692.0 0 64 186 94 692.0 0 94 187 143 692.0 0 143 188 1716 692.0 0 1716 189 821 692.0 0 821 190 1811 692.0 0 1811 191 113 692.0 0 113 192 32 692.0 0 32 193 63 692.0 0 63 194 55 692.0 0 55 195 98 692.0 0 98 196 216 692.0 0 216 197 171 692.0 0 171 198 203 692.0 0 203 199 442 692.0 0 442 200 464 692.0 0 464 201 3194 692.0 0 3194 202 2038 692.0 0 2038 203 7911 692.0 0 7911 204 218 692.0 0 218 205 64 692.0 0 64 206 72 692.0 0 72 207 48 692.0 0 48 208 49 692.0 0 49 209 53 692.0 0 53 210 63 692.0 0 63 211 27 692.0 0 27 212 32 692.0 0 32 213 47 692.0 0 47 214 59 692.0 0 59 215 88 692.0 0 88 216 56 692.0 0 56 217 34 692.0 0 34 218 33 692.0 0 33 219 28 692.0 0 28 220 39 692.0 0 39 221 46 692.0 0 46 222 44 692.0 0 44 223 57 692.0 0 57 224 122 692.0 0 122 225 91 692.0 0 91 226 250 692.0 0 250 227 94 692.0 0 94 228 271 692.0 0 271 229 510 692.0 0 510 230 109 692.0 0 109 231 88 692.0 0 88 232 43 692.0 0 43 233 34 692.0 0 34 234 42 692.0 0 42 235 49 692.0 0 49 236 57 692.0 0 57 237 47 692.0 0 47 238 65 692.0 0 65 239 57 692.0 0 57 240 41 692.0 0 41 241 71 692.0 0 71 242 69 692.0 0 69 243 66 692.0 0 66 244 63 692.0 0 63 245 86 692.0 0 86 246 51 692.0 0 51 247 88 692.0 0 88 248 84 692.0 0 84 249 111 692.0 0 111 250 137 692.0 0 137 251 127 692.0 0 127 252 108 692.0 0 108 253 108 692.0 0 108 254 130 692.0 0 130 255 39 692.0 0 39 256 34 692.0 0 34 257 45 692.0 0 45 258 71 692.0 0 71 259 77 692.0 0 77 260 76 692.0 0 76 261 62 692.0 0 62 262 117 692.0 0 117 263 52 692.0 0 52 264 78 692.0 0 78 265 65 692.0 0 65 266 50 692.0 0 50 267 52 692.0 0 52 268 67 692.0 0 67 269 452 692.0 0 452 270 351 692.0 0 351 271 309 692.0 0 309 272 89 692.0 0 89 273 87 692.0 0 87 274 210 692.0 0 210 275 186 692.0 0 186 276 122 692.0 0 122 277 193 692.0 0 193 278 88 692.0 0 88 279 56 692.0 0 56 280 182 692.0 0 182 281 107 692.0 0 107 282 425 692.0 0 425 283 182 692.0 0 182 284 205 692.0 0 205 285 103 692.0 0 103 286 96 692.0 0 96 287 68 692.0 0 68 288 157 692.0 0 157 289 143 692.0 0 143 290 134 692.0 0 134 291 740 692.0 0 740 292 345 692.0 0 345 293 635 692.0 0 635 294 1119 692.0 0 1119 295 435 692.0 0 435 296 538 692.0 0 538 297 105 692.0 0 105 298 60 692.0 0 60 299 183 692.0 0 183 300 131 692.0 0 131 301 96 692.0 0 96 Finished in 3.46 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 73,871 Reads with adapters: 18,593 (25.2%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 18,557 (25.1%) Total basepairs processed: 16,606,637 bp Total written (filtered): 5,047,142 bp (30.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18593 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 14 0.0 3 0 0 10 1 3 19 214 0.0 3 0 191 13 9 1 20 15499 0.0 4 14359 890 185 47 18 21 213 0.0 4 86 95 19 10 3 22 2481 0.0 4 2281 146 31 17 6 23 86 0.0 4 2 5 0 60 19 24 4 0.0 4 0 0 0 3 1 25 22 0.0 4 1 0 0 19 2 27 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 42 2 0.0 4 0 0 0 0 2 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 85 3 0.0 4 0 1 1 0 1 87 1 0.0 4 0 0 1 88 1 0.0 4 0 1 89 6 0.0 4 0 0 0 0 6 91 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 1 118 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 129 3 0.0 4 0 0 0 0 3 137 1 0.0 4 1 138 1 0.0 4 1 150 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 1 242 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 18,557 Reads with adapters: 4,795 (25.8%) Reads written (passing filters): 18,557 (100.0%) Total basepairs processed: 5,047,142 bp Total written (filtered): 4,992,303 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4795 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 7.7% G: 87.4% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 290.0 0 5 4 4 72.5 0 4 7 2 1.1 0 2 8 1 0.3 0 1 9 1438 0.1 0 162 1276 10 2660 0.0 1 218 2442 11 609 0.0 1 28 581 12 20 0.0 1 1 19 13 15 0.0 1 0 15 14 1 0.0 1 0 1 150 3 0.0 1 3 151 1 0.0 1 1 208 32 0.0 1 26 6 209 2 0.0 1 2 210 1 0.0 1 1 211 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.