This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.61 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 92,229 (3.3%) Reads that were too short: 22,249 (0.8%) Reads written (passing filters): 69,980 (2.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 16,056,027 bp (1.9%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 92229 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4883 692.0 0 4883 7 17264 692.0 0 17264 8 746 692.0 0 746 9 16618 692.0 0 16618 10 35 692.0 0 35 11 27 692.0 0 27 12 11 692.0 0 11 13 11 692.0 0 11 14 18 692.0 0 18 15 17 692.0 0 17 16 15 692.0 0 15 17 14 692.0 0 14 18 28 692.0 0 28 19 20 692.0 0 20 20 17 692.0 0 17 21 16 692.0 0 16 22 18 692.0 0 18 23 20 692.0 0 20 24 8 692.0 0 8 25 12 692.0 0 12 26 16 692.0 0 16 27 22 692.0 0 22 28 32 692.0 0 32 29 27 692.0 0 27 30 21 692.0 0 21 31 22 692.0 0 22 32 22 692.0 0 22 33 14 692.0 0 14 34 9 692.0 0 9 35 25 692.0 0 25 36 27 692.0 0 27 37 23 692.0 0 23 38 20 692.0 0 20 39 18 692.0 0 18 40 27 692.0 0 27 41 17 692.0 0 17 42 17 692.0 0 17 43 7 692.0 0 7 44 17 692.0 0 17 45 24 692.0 0 24 46 17 692.0 0 17 47 21 692.0 0 21 48 15 692.0 0 15 49 21 692.0 0 21 50 26 692.0 0 26 51 19 692.0 0 19 52 14 692.0 0 14 53 15 692.0 0 15 54 7 692.0 0 7 55 56 692.0 0 56 56 31 692.0 0 31 57 54 692.0 0 54 58 36 692.0 0 36 59 42 692.0 0 42 60 17 692.0 0 17 61 27 692.0 0 27 62 21 692.0 0 21 63 15 692.0 0 15 64 26 692.0 0 26 65 21 692.0 0 21 66 20 692.0 0 20 67 26 692.0 0 26 68 16 692.0 0 16 69 14 692.0 0 14 70 12 692.0 0 12 71 32 692.0 0 32 72 12 692.0 0 12 73 28 692.0 0 28 74 18 692.0 0 18 75 49 692.0 0 49 76 82 692.0 0 82 77 30 692.0 0 30 78 22 692.0 0 22 79 51 692.0 0 51 80 18 692.0 0 18 81 26 692.0 0 26 82 16 692.0 0 16 83 27 692.0 0 27 84 26 692.0 0 26 85 35 692.0 0 35 86 31 692.0 0 31 87 24 692.0 0 24 88 27 692.0 0 27 89 22 692.0 0 22 90 42 692.0 0 42 91 26 692.0 0 26 92 23 692.0 0 23 93 21 692.0 0 21 94 29 692.0 0 29 95 56 692.0 0 56 96 53 692.0 0 53 97 36 692.0 0 36 98 32 692.0 0 32 99 16 692.0 0 16 100 21 692.0 0 21 101 26 692.0 0 26 102 23 692.0 0 23 103 40 692.0 0 40 104 26 692.0 0 26 105 132 692.0 0 132 106 95 692.0 0 95 107 113 692.0 0 113 108 14 692.0 0 14 109 19 692.0 0 19 110 26 692.0 0 26 111 27 692.0 0 27 112 28 692.0 0 28 113 24 692.0 0 24 114 32 692.0 0 32 115 44 692.0 0 44 116 128 692.0 0 128 117 34 692.0 0 34 118 34 692.0 0 34 119 27 692.0 0 27 120 29 692.0 0 29 121 23 692.0 0 23 122 17 692.0 0 17 123 35 692.0 0 35 124 34 692.0 0 34 125 27 692.0 0 27 126 89 692.0 0 89 127 63 692.0 0 63 128 46 692.0 0 46 129 62 692.0 0 62 130 131 692.0 0 131 131 41 692.0 0 41 132 98 692.0 0 98 133 87 692.0 0 87 134 201 692.0 0 201 135 63 692.0 0 63 136 70 692.0 0 70 137 133 692.0 0 133 138 51 692.0 0 51 139 93 692.0 0 93 140 121 692.0 0 121 141 93 692.0 0 93 142 125 692.0 0 125 143 107 692.0 0 107 144 136 692.0 0 136 145 125 692.0 0 125 146 166 692.0 0 166 147 2263 692.0 0 2263 148 1847 692.0 0 1847 149 10593 692.0 0 10593 150 206 692.0 0 206 151 104 692.0 0 104 152 131 692.0 0 131 153 48 692.0 0 48 154 114 692.0 0 114 155 81 692.0 0 81 156 55 692.0 0 55 157 23 692.0 0 23 158 20 692.0 0 20 159 41 692.0 0 41 160 41 692.0 0 41 161 23 692.0 0 23 162 27 692.0 0 27 163 26 692.0 0 26 164 29 692.0 0 29 165 17 692.0 0 17 166 22 692.0 0 22 167 45 692.0 0 45 168 84 692.0 0 84 169 52 692.0 0 52 170 143 692.0 0 143 171 84 692.0 0 84 172 50 692.0 0 50 173 41 692.0 0 41 174 126 692.0 0 126 175 92 692.0 0 92 176 24 692.0 0 24 177 28 692.0 0 28 178 39 692.0 0 39 179 34 692.0 0 34 180 31 692.0 0 31 181 20 692.0 0 20 182 43 692.0 0 43 183 53 692.0 0 53 184 115 692.0 0 115 185 41 692.0 0 41 186 78 692.0 0 78 187 70 692.0 0 70 188 880 692.0 0 880 189 414 692.0 0 414 190 951 692.0 0 951 191 52 692.0 0 52 192 25 692.0 0 25 193 50 692.0 0 50 194 42 692.0 0 42 195 69 692.0 0 69 196 157 692.0 0 157 197 138 692.0 0 138 198 192 692.0 0 192 199 328 692.0 0 328 200 406 692.0 0 406 201 4389 692.0 0 4389 202 2649 692.0 0 2649 203 13846 692.0 0 13846 204 142 692.0 0 142 205 31 692.0 0 31 206 29 692.0 0 29 207 23 692.0 0 23 208 34 692.0 0 34 209 38 692.0 0 38 210 27 692.0 0 27 211 23 692.0 0 23 212 23 692.0 0 23 213 36 692.0 0 36 214 25 692.0 0 25 215 54 692.0 0 54 216 34 692.0 0 34 217 21 692.0 0 21 218 15 692.0 0 15 219 18 692.0 0 18 220 20 692.0 0 20 221 30 692.0 0 30 222 19 692.0 0 19 223 31 692.0 0 31 224 37 692.0 0 37 225 54 692.0 0 54 226 77 692.0 0 77 227 50 692.0 0 50 228 187 692.0 0 187 229 683 692.0 0 683 230 51 692.0 0 51 231 31 692.0 0 31 232 36 692.0 0 36 233 13 692.0 0 13 234 27 692.0 0 27 235 20 692.0 0 20 236 25 692.0 0 25 237 31 692.0 0 31 238 46 692.0 0 46 239 21 692.0 0 21 240 26 692.0 0 26 241 39 692.0 0 39 242 65 692.0 0 65 243 41 692.0 0 41 244 33 692.0 0 33 245 47 692.0 0 47 246 18 692.0 0 18 247 32 692.0 0 32 248 26 692.0 0 26 249 34 692.0 0 34 250 51 692.0 0 51 251 54 692.0 0 54 252 43 692.0 0 43 253 38 692.0 0 38 254 88 692.0 0 88 255 14 692.0 0 14 256 17 692.0 0 17 257 34 692.0 0 34 258 40 692.0 0 40 259 36 692.0 0 36 260 29 692.0 0 29 261 22 692.0 0 22 262 39 692.0 0 39 263 17 692.0 0 17 264 23 692.0 0 23 265 21 692.0 0 21 266 26 692.0 0 26 267 29 692.0 0 29 268 34 692.0 0 34 269 143 692.0 0 143 270 91 692.0 0 91 271 104 692.0 0 104 272 34 692.0 0 34 273 77 692.0 0 77 274 69 692.0 0 69 275 61 692.0 0 61 276 61 692.0 0 61 277 90 692.0 0 90 278 48 692.0 0 48 279 38 692.0 0 38 280 49 692.0 0 49 281 32 692.0 0 32 282 99 692.0 0 99 283 72 692.0 0 72 284 78 692.0 0 78 285 34 692.0 0 34 286 35 692.0 0 35 287 30 692.0 0 30 288 49 692.0 0 49 289 41 692.0 0 41 290 66 692.0 0 66 291 205 692.0 0 205 292 110 692.0 0 110 293 195 692.0 0 195 294 329 692.0 0 329 295 130 692.0 0 130 296 105 692.0 0 105 297 38 692.0 0 38 298 17 692.0 0 17 299 60 692.0 0 60 300 57 692.0 0 57 301 29 692.0 0 29 Finished in 3.00 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 69,980 Reads with adapters: 18,286 (26.1%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 18,254 (26.1%) Total basepairs processed: 16,056,027 bp Total written (filtered): 5,043,043 bp (31.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18286 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 126 0.0 3 0 123 3 17 14778 0.0 3 14450 316 11 1 18 82 0.0 3 19 62 1 19 3172 0.0 3 3097 56 7 12 20 21 0.0 3 1 7 1 12 21 1 0.0 3 0 0 0 1 22 8 0.0 3 1 0 0 7 23 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 43 6 0.0 3 0 0 0 6 45 33 0.0 3 0 0 0 33 46 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 57 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 1 79 11 0.0 3 0 0 0 11 81 1 0.0 3 0 1 82 1 0.0 3 0 0 0 1 83 3 0.0 3 2 0 1 84 1 0.0 3 1 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 3 0.0 3 0 0 0 3 88 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 155 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.65 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 18,254 Reads with adapters: 105 (0.6%) Reads written (passing filters): 18,254 (100.0%) Total basepairs processed: 5,043,043 bp Total written (filtered): 5,042,706 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 105 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.0% C: 21.9% G: 25.7% T: 33.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 85 285.2 0 85 4 19 71.3 0 19 6 1 4.5 0 1