This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.20 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 448,955 (15.8%) Reads that were too short: 40,672 (1.4%) Reads written (passing filters): 408,283 (14.4%) Total basepairs processed: 853,219,115 bp Total written (filtered): 115,936,474 bp (13.6%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 448955 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2737 692.0 0 2737 7 199499 692.0 0 199499 8 2411 692.0 0 2411 9 174741 692.0 0 174741 10 115 692.0 0 115 11 11 692.0 0 11 12 10 692.0 0 10 13 10 692.0 0 10 14 16 692.0 0 16 15 15 692.0 0 15 16 17 692.0 0 17 17 16 692.0 0 16 18 13 692.0 0 13 19 4 692.0 0 4 20 11 692.0 0 11 21 13 692.0 0 13 22 11 692.0 0 11 23 12 692.0 0 12 24 10 692.0 0 10 25 8 692.0 0 8 26 13 692.0 0 13 27 13 692.0 0 13 28 28 692.0 0 28 29 20 692.0 0 20 30 19 692.0 0 19 31 25 692.0 0 25 32 19 692.0 0 19 33 23 692.0 0 23 34 11 692.0 0 11 35 13 692.0 0 13 36 10 692.0 0 10 37 17 692.0 0 17 38 11 692.0 0 11 39 29 692.0 0 29 40 27 692.0 0 27 41 148 692.0 0 148 42 60 692.0 0 60 43 173 692.0 0 173 44 33 692.0 0 33 45 35 692.0 0 35 46 18 692.0 0 18 47 22 692.0 0 22 48 17 692.0 0 17 49 22 692.0 0 22 50 26 692.0 0 26 51 23 692.0 0 23 52 42 692.0 0 42 53 10 692.0 0 10 54 14 692.0 0 14 55 9 692.0 0 9 56 12 692.0 0 12 57 45 692.0 0 45 58 18 692.0 0 18 59 19 692.0 0 19 60 24 692.0 0 24 61 11 692.0 0 11 62 20 692.0 0 20 63 15 692.0 0 15 64 12 692.0 0 12 65 17 692.0 0 17 66 19 692.0 0 19 67 52 692.0 0 52 68 19 692.0 0 19 69 73 692.0 0 73 70 29 692.0 0 29 71 48 692.0 0 48 72 51 692.0 0 51 73 48 692.0 0 48 74 56 692.0 0 56 75 59 692.0 0 59 76 105 692.0 0 105 77 196 692.0 0 196 78 185 692.0 0 185 79 163 692.0 0 163 80 142 692.0 0 142 81 141 692.0 0 141 82 169 692.0 0 169 83 204 692.0 0 204 84 185 692.0 0 185 85 101 692.0 0 101 86 84 692.0 0 84 87 74 692.0 0 74 88 73 692.0 0 73 89 84 692.0 0 84 90 83 692.0 0 83 91 105 692.0 0 105 92 82 692.0 0 82 93 83 692.0 0 83 94 80 692.0 0 80 95 78 692.0 0 78 96 56 692.0 0 56 97 60 692.0 0 60 98 78 692.0 0 78 99 78 692.0 0 78 100 71 692.0 0 71 101 81 692.0 0 81 102 90 692.0 0 90 103 156 692.0 0 156 104 194 692.0 0 194 105 221 692.0 0 221 106 223 692.0 0 223 107 238 692.0 0 238 108 162 692.0 0 162 109 174 692.0 0 174 110 159 692.0 0 159 111 169 692.0 0 169 112 147 692.0 0 147 113 235 692.0 0 235 114 168 692.0 0 168 115 186 692.0 0 186 116 203 692.0 0 203 117 141 692.0 0 141 118 214 692.0 0 214 119 187 692.0 0 187 120 266 692.0 0 266 121 166 692.0 0 166 122 196 692.0 0 196 123 183 692.0 0 183 124 254 692.0 0 254 125 250 692.0 0 250 126 266 692.0 0 266 127 167 692.0 0 167 128 261 692.0 0 261 129 220 692.0 0 220 130 199 692.0 0 199 131 216 692.0 0 216 132 255 692.0 0 255 133 1776 692.0 0 1776 134 1099 692.0 0 1099 135 1674 692.0 0 1674 136 178 692.0 0 178 137 158 692.0 0 158 138 147 692.0 0 147 139 148 692.0 0 148 140 177 692.0 0 177 141 183 692.0 0 183 142 140 692.0 0 140 143 181 692.0 0 181 144 189 692.0 0 189 145 165 692.0 0 165 146 181 692.0 0 181 147 166 692.0 0 166 148 168 692.0 0 168 149 147 692.0 0 147 150 144 692.0 0 144 151 131 692.0 0 131 152 148 692.0 0 148 153 154 692.0 0 154 154 175 692.0 0 175 155 156 692.0 0 156 156 138 692.0 0 138 157 159 692.0 0 159 158 176 692.0 0 176 159 222 692.0 0 222 160 300 692.0 0 300 161 286 692.0 0 286 162 697 692.0 0 697 163 399 692.0 0 399 164 585 692.0 0 585 165 209 692.0 0 209 166 163 692.0 0 163 167 141 692.0 0 141 168 140 692.0 0 140 169 152 692.0 0 152 170 156 692.0 0 156 171 175 692.0 0 175 172 236 692.0 0 236 173 184 692.0 0 184 174 163 692.0 0 163 175 176 692.0 0 176 176 117 692.0 0 117 177 115 692.0 0 115 178 162 692.0 0 162 179 218 692.0 0 218 180 200 692.0 0 200 181 139 692.0 0 139 182 220 692.0 0 220 183 456 692.0 0 456 184 258 692.0 0 258 185 241 692.0 0 241 186 204 692.0 0 204 187 233 692.0 0 233 188 218 692.0 0 218 189 254 692.0 0 254 190 210 692.0 0 210 191 227 692.0 0 227 192 231 692.0 0 231 193 195 692.0 0 195 194 163 692.0 0 163 195 131 692.0 0 131 196 170 692.0 0 170 197 192 692.0 0 192 198 186 692.0 0 186 199 193 692.0 0 193 200 222 692.0 0 222 201 222 692.0 0 222 202 220 692.0 0 220 203 216 692.0 0 216 204 176 692.0 0 176 205 179 692.0 0 179 206 176 692.0 0 176 207 229 692.0 0 229 208 209 692.0 0 209 209 287 692.0 0 287 210 260 692.0 0 260 211 557 692.0 0 557 212 473 692.0 0 473 213 3781 692.0 0 3781 214 7020 692.0 0 7020 215 3969 692.0 0 3969 216 471 692.0 0 471 217 243 692.0 0 243 218 284 692.0 0 284 219 255 692.0 0 255 220 238 692.0 0 238 221 261 692.0 0 261 222 281 692.0 0 281 223 214 692.0 0 214 224 272 692.0 0 272 225 575 692.0 0 575 226 565 692.0 0 565 227 541 692.0 0 541 228 321 692.0 0 321 229 309 692.0 0 309 230 296 692.0 0 296 231 189 692.0 0 189 232 260 692.0 0 260 233 193 692.0 0 193 234 208 692.0 0 208 235 204 692.0 0 204 236 196 692.0 0 196 237 243 692.0 0 243 238 191 692.0 0 191 239 185 692.0 0 185 240 203 692.0 0 203 241 196 692.0 0 196 242 169 692.0 0 169 243 208 692.0 0 208 244 156 692.0 0 156 245 194 692.0 0 194 246 189 692.0 0 189 247 247 692.0 0 247 248 242 692.0 0 242 249 300 692.0 0 300 250 416 692.0 0 416 251 332 692.0 0 332 252 460 692.0 0 460 253 280 692.0 0 280 254 282 692.0 0 282 255 221 692.0 0 221 256 236 692.0 0 236 257 232 692.0 0 232 258 208 692.0 0 208 259 210 692.0 0 210 260 228 692.0 0 228 261 209 692.0 0 209 262 202 692.0 0 202 263 206 692.0 0 206 264 252 692.0 0 252 265 246 692.0 0 246 266 235 692.0 0 235 267 188 692.0 0 188 268 207 692.0 0 207 269 224 692.0 0 224 270 231 692.0 0 231 271 257 692.0 0 257 272 201 692.0 0 201 273 246 692.0 0 246 274 283 692.0 0 283 275 249 692.0 0 249 276 291 692.0 0 291 277 305 692.0 0 305 278 306 692.0 0 306 279 283 692.0 0 283 280 230 692.0 0 230 281 311 692.0 0 311 282 359 692.0 0 359 283 281 692.0 0 281 284 283 692.0 0 283 285 264 692.0 0 264 286 287 692.0 0 287 287 254 692.0 0 254 288 206 692.0 0 206 289 366 692.0 0 366 290 283 692.0 0 283 291 348 692.0 0 348 292 236 692.0 0 236 293 221 692.0 0 221 294 232 692.0 0 232 295 248 692.0 0 248 296 295 692.0 0 295 297 244 692.0 0 244 298 242 692.0 0 242 299 214 692.0 0 214 300 246 692.0 0 246 301 445 692.0 0 445 Finished in 17.59 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 408,283 Reads with adapters: 179,047 (43.9%) Reads that were too short: 107 (0.0%) Reads written (passing filters): 178,940 (43.8%) Total basepairs processed: 115,936,474 bp Total written (filtered): 49,207,350 bp (42.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 179047 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 39 0.0 3 0 0 31 8 16 968 0.0 3 0 946 20 2 17 177155 0.0 3 173222 3760 157 16 18 489 0.0 3 42 422 22 3 19 145 0.0 3 9 0 119 17 20 94 0.0 3 0 1 1 92 21 2 0.0 3 1 0 0 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 32 2 0.0 3 1 0 0 1 33 2 0.0 3 0 0 1 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 1 43 1 0.0 3 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 1 0.0 3 1 88 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 153 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 155 4 0.0 3 0 0 0 4 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 184 1 0.0 3 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 1 0 0 1 195 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 25 0.0 3 0 0 2 23 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 276 1 0.0 3 0 1 277 1 0.0 3 0 0 1 279 7 0.0 3 0 0 0 7 280 6 0.0 3 0 0 0 6 281 8 0.0 3 0 0 0 8 282 6 0.0 3 0 0 0 6 283 3 0.0 3 0 0 1 2 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.51 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 178,940 Reads with adapters: 13,540 (7.6%) Reads written (passing filters): 178,940 (100.0%) Total basepairs processed: 49,207,350 bp Total written (filtered): 49,166,510 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13540 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.0% C: 6.2% G: 13.9% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 13362 2795.9 0 13362 4 170 699.0 0 170 5 2 174.7 0 2 9 1 0.7 0 0 1 10 3 0.2 1 0 3 12 1 0.0 1 0 1 13 1 0.0 1 0 1