This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.64 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 663,756 (23.4%) Reads that were too short: 592,697 (20.9%) Reads written (passing filters): 71,059 (2.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 17,665,044 bp (2.1%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 663756 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 545 692.0 0 545 7 1273 692.0 0 1273 8 25991 692.0 0 25991 9 21174 692.0 0 21174 10 70 692.0 0 70 11 35 692.0 0 35 12 33 692.0 0 33 13 56 692.0 0 56 14 58 692.0 0 58 15 25 692.0 0 25 16 44 692.0 0 44 17 33 692.0 0 33 18 69 692.0 0 69 19 45 692.0 0 45 20 119 692.0 0 119 21 54 692.0 0 54 22 106 692.0 0 106 23 43 692.0 0 43 24 49 692.0 0 49 25 45 692.0 0 45 26 59 692.0 0 59 27 48 692.0 0 48 28 49 692.0 0 49 29 37 692.0 0 37 30 28 692.0 0 28 31 39 692.0 0 39 32 42 692.0 0 42 33 138 692.0 0 138 34 55 692.0 0 55 35 63 692.0 0 63 36 76 692.0 0 76 37 82 692.0 0 82 38 55 692.0 0 55 39 27 692.0 0 27 40 29 692.0 0 29 41 12 692.0 0 12 42 40 692.0 0 40 43 39 692.0 0 39 44 46 692.0 0 46 45 36 692.0 0 36 46 33 692.0 0 33 47 57 692.0 0 57 48 36 692.0 0 36 49 65 692.0 0 65 50 37 692.0 0 37 51 44 692.0 0 44 52 28 692.0 0 28 53 37 692.0 0 37 54 35 692.0 0 35 55 23 692.0 0 23 56 25 692.0 0 25 57 43 692.0 0 43 58 24 692.0 0 24 59 27 692.0 0 27 60 43 692.0 0 43 61 34 692.0 0 34 62 31 692.0 0 31 63 69 692.0 0 69 64 62 692.0 0 62 65 39 692.0 0 39 66 62 692.0 0 62 67 56 692.0 0 56 68 44 692.0 0 44 69 33 692.0 0 33 70 22 692.0 0 22 71 35 692.0 0 35 72 35 692.0 0 35 73 111 692.0 0 111 74 72 692.0 0 72 75 40 692.0 0 40 76 49 692.0 0 49 77 62 692.0 0 62 78 47 692.0 0 47 79 41 692.0 0 41 80 40 692.0 0 40 81 35 692.0 0 35 82 46 692.0 0 46 83 29 692.0 0 29 84 55 692.0 0 55 85 50 692.0 0 50 86 37 692.0 0 37 87 58 692.0 0 58 88 69 692.0 0 69 89 89 692.0 0 89 90 61 692.0 0 61 91 86 692.0 0 86 92 107 692.0 0 107 93 81 692.0 0 81 94 82 692.0 0 82 95 120 692.0 0 120 96 46 692.0 0 46 97 75 692.0 0 75 98 53 692.0 0 53 99 79 692.0 0 79 100 46 692.0 0 46 101 40 692.0 0 40 102 30 692.0 0 30 103 38 692.0 0 38 104 33 692.0 0 33 105 36 692.0 0 36 106 30 692.0 0 30 107 50 692.0 0 50 108 39 692.0 0 39 109 29 692.0 0 29 110 36 692.0 0 36 111 34 692.0 0 34 112 35 692.0 0 35 113 46 692.0 0 46 114 34 692.0 0 34 115 29 692.0 0 29 116 59 692.0 0 59 117 34 692.0 0 34 118 48 692.0 0 48 119 44 692.0 0 44 120 47 692.0 0 47 121 55 692.0 0 55 122 73 692.0 0 73 123 47 692.0 0 47 124 154 692.0 0 154 125 236 692.0 0 236 126 219 692.0 0 219 127 241 692.0 0 241 128 171 692.0 0 171 129 48 692.0 0 48 130 68 692.0 0 68 131 264 692.0 0 264 132 503 692.0 0 503 133 189 692.0 0 189 134 126 692.0 0 126 135 66 692.0 0 66 136 47 692.0 0 47 137 42 692.0 0 42 138 45 692.0 0 45 139 39 692.0 0 39 140 44 692.0 0 44 141 39 692.0 0 39 142 41 692.0 0 41 143 43 692.0 0 43 144 61 692.0 0 61 145 58 692.0 0 58 146 92 692.0 0 92 147 99 692.0 0 99 148 73 692.0 0 73 149 35 692.0 0 35 150 45 692.0 0 45 151 39 692.0 0 39 152 48 692.0 0 48 153 53 692.0 0 53 154 66 692.0 0 66 155 106 692.0 0 106 156 100 692.0 0 100 157 32 692.0 0 32 158 36 692.0 0 36 159 35 692.0 0 35 160 44 692.0 0 44 161 33 692.0 0 33 162 27 692.0 0 27 163 37 692.0 0 37 164 40 692.0 0 40 165 46 692.0 0 46 166 69 692.0 0 69 167 60 692.0 0 60 168 44 692.0 0 44 169 32 692.0 0 32 170 33 692.0 0 33 171 30 692.0 0 30 172 53 692.0 0 53 173 83 692.0 0 83 174 196 692.0 0 196 175 207 692.0 0 207 176 214 692.0 0 214 177 59 692.0 0 59 178 74 692.0 0 74 179 222 692.0 0 222 180 168 692.0 0 168 181 365 692.0 0 365 182 107 692.0 0 107 183 77 692.0 0 77 184 57 692.0 0 57 185 241 692.0 0 241 186 152 692.0 0 152 187 307 692.0 0 307 188 93 692.0 0 93 189 86 692.0 0 86 190 101 692.0 0 101 191 146 692.0 0 146 192 425 692.0 0 425 193 3022 692.0 0 3022 194 1125 692.0 0 1125 195 1808 692.0 0 1808 196 121 692.0 0 121 197 106 692.0 0 106 198 112 692.0 0 112 199 465 692.0 0 465 200 760 692.0 0 760 201 1351 692.0 0 1351 202 303 692.0 0 303 203 91 692.0 0 91 204 46 692.0 0 46 205 91 692.0 0 91 206 207 692.0 0 207 207 143 692.0 0 143 208 99 692.0 0 99 209 63 692.0 0 63 210 96 692.0 0 96 211 130 692.0 0 130 212 146 692.0 0 146 213 225 692.0 0 225 214 116 692.0 0 116 215 124 692.0 0 124 216 113 692.0 0 113 217 113 692.0 0 113 218 157 692.0 0 157 219 292 692.0 0 292 220 324 692.0 0 324 221 482 692.0 0 482 222 639 692.0 0 639 223 811 692.0 0 811 224 1261 692.0 0 1261 225 420 692.0 0 420 226 362 692.0 0 362 227 648 692.0 0 648 228 377 692.0 0 377 229 449 692.0 0 449 230 690 692.0 0 690 231 951 692.0 0 951 232 2551 692.0 0 2551 233 2592 692.0 0 2592 234 8418 692.0 0 8418 235 1992 692.0 0 1992 236 2033 692.0 0 2033 237 39324 692.0 0 39324 238 12006 692.0 0 12006 239 41771 692.0 0 41771 240 1756 692.0 0 1756 241 1867 692.0 0 1867 242 709 692.0 0 709 243 1238 692.0 0 1238 244 1824 692.0 0 1824 245 895 692.0 0 895 246 792 692.0 0 792 247 1127 692.0 0 1127 248 847 692.0 0 847 249 1061 692.0 0 1061 250 1871 692.0 0 1871 251 1698 692.0 0 1698 252 2558 692.0 0 2558 253 2462 692.0 0 2462 254 3962 692.0 0 3962 255 4019 692.0 0 4019 256 5268 692.0 0 5268 257 107027 692.0 0 107027 258 71737 692.0 0 71737 259 244905 692.0 0 244905 260 9211 692.0 0 9211 261 128 692.0 0 128 262 122 692.0 0 122 263 45 692.0 0 45 264 35 692.0 0 35 265 31 692.0 0 31 266 62 692.0 0 62 267 60 692.0 0 60 268 200 692.0 0 200 269 255 692.0 0 255 270 114 692.0 0 114 271 62 692.0 0 62 272 105 692.0 0 105 273 57 692.0 0 57 274 53 692.0 0 53 275 73 692.0 0 73 276 63 692.0 0 63 277 40 692.0 0 40 278 43 692.0 0 43 279 74 692.0 0 74 280 56 692.0 0 56 281 68 692.0 0 68 282 112 692.0 0 112 283 60 692.0 0 60 284 80 692.0 0 80 285 99 692.0 0 99 286 93 692.0 0 93 287 188 692.0 0 188 288 103 692.0 0 103 289 212 692.0 0 212 290 216 692.0 0 216 291 661 692.0 0 661 292 381 692.0 0 381 293 411 692.0 0 411 294 127 692.0 0 127 295 108 692.0 0 108 296 107 692.0 0 107 297 95 692.0 0 95 298 41 692.0 0 41 299 49 692.0 0 49 300 166 692.0 0 166 301 152 692.0 0 152 Finished in 3.66 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 71,059 Reads with adapters: 23,615 (33.2%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 23,586 (33.2%) Total basepairs processed: 17,665,044 bp Total written (filtered): 6,400,414 bp (36.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23615 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 2 5 18 18 0.0 3 0 0 9 6 3 19 559 0.0 3 0 492 31 30 6 20 22612 0.0 4 20908 1315 282 80 27 21 62 0.0 4 9 42 7 3 1 22 15 0.0 4 1 0 6 3 5 23 145 0.0 4 1 1 0 100 43 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 65 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 1 96 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 158 0.0 4 0 0 0 0 158 157 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 1 239 1 0.0 4 0 0 0 0 1 249 2 0.0 4 0 0 1 0 1 250 1 0.0 4 0 0 1 252 2 0.0 4 0 0 0 1 1 253 1 0.0 4 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 23,586 Reads with adapters: 5,775 (24.5%) Reads written (passing filters): 23,586 (100.0%) Total basepairs processed: 6,400,414 bp Total written (filtered): 6,339,666 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5775 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 8.2% G: 87.0% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 368.5 0 12 4 4 92.1 0 4 6 1 5.8 0 1 7 1 1.4 0 1 8 1 0.4 0 1 9 1344 0.1 0 149 1195 10 3668 0.0 1 327 3341 11 687 0.0 1 23 664 12 19 0.0 1 0 19 13 17 0.0 1 0 17 14 1 0.0 1 0 1 139 1 0.0 1 1 150 2 0.0 1 1 1 151 2 0.0 1 2 208 13 0.0 1 11 2 211 1 0.0 1 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.