This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.64 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 670,338 (23.6%) Reads that were too short: 598,991 (21.1%) Reads written (passing filters): 71,347 (2.5%) Total basepairs processed: 853,219,115 bp Total written (filtered): 18,089,901 bp (2.1%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 670338 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 561 692.0 0 561 7 1027 692.0 0 1027 8 25269 692.0 0 25269 9 23933 692.0 0 23933 10 75 692.0 0 75 11 52 692.0 0 52 12 46 692.0 0 46 13 45 692.0 0 45 14 58 692.0 0 58 15 28 692.0 0 28 16 52 692.0 0 52 17 59 692.0 0 59 18 72 692.0 0 72 19 44 692.0 0 44 20 72 692.0 0 72 21 33 692.0 0 33 22 75 692.0 0 75 23 60 692.0 0 60 24 53 692.0 0 53 25 62 692.0 0 62 26 72 692.0 0 72 27 39 692.0 0 39 28 48 692.0 0 48 29 47 692.0 0 47 30 22 692.0 0 22 31 42 692.0 0 42 32 36 692.0 0 36 33 43 692.0 0 43 34 41 692.0 0 41 35 53 692.0 0 53 36 58 692.0 0 58 37 86 692.0 0 86 38 56 692.0 0 56 39 37 692.0 0 37 40 40 692.0 0 40 41 35 692.0 0 35 42 36 692.0 0 36 43 32 692.0 0 32 44 46 692.0 0 46 45 38 692.0 0 38 46 40 692.0 0 40 47 63 692.0 0 63 48 37 692.0 0 37 49 60 692.0 0 60 50 60 692.0 0 60 51 59 692.0 0 59 52 35 692.0 0 35 53 50 692.0 0 50 54 40 692.0 0 40 55 38 692.0 0 38 56 35 692.0 0 35 57 57 692.0 0 57 58 27 692.0 0 27 59 39 692.0 0 39 60 38 692.0 0 38 61 48 692.0 0 48 62 33 692.0 0 33 63 83 692.0 0 83 64 84 692.0 0 84 65 28 692.0 0 28 66 53 692.0 0 53 67 86 692.0 0 86 68 44 692.0 0 44 69 51 692.0 0 51 70 28 692.0 0 28 71 55 692.0 0 55 72 50 692.0 0 50 73 138 692.0 0 138 74 48 692.0 0 48 75 55 692.0 0 55 76 50 692.0 0 50 77 65 692.0 0 65 78 50 692.0 0 50 79 51 692.0 0 51 80 32 692.0 0 32 81 45 692.0 0 45 82 50 692.0 0 50 83 42 692.0 0 42 84 67 692.0 0 67 85 55 692.0 0 55 86 58 692.0 0 58 87 59 692.0 0 59 88 65 692.0 0 65 89 97 692.0 0 97 90 71 692.0 0 71 91 106 692.0 0 106 92 123 692.0 0 123 93 62 692.0 0 62 94 70 692.0 0 70 95 54 692.0 0 54 96 27 692.0 0 27 97 57 692.0 0 57 98 49 692.0 0 49 99 55 692.0 0 55 100 45 692.0 0 45 101 32 692.0 0 32 102 52 692.0 0 52 103 40 692.0 0 40 104 40 692.0 0 40 105 39 692.0 0 39 106 57 692.0 0 57 107 41 692.0 0 41 108 47 692.0 0 47 109 39 692.0 0 39 110 46 692.0 0 46 111 28 692.0 0 28 112 36 692.0 0 36 113 48 692.0 0 48 114 39 692.0 0 39 115 42 692.0 0 42 116 66 692.0 0 66 117 49 692.0 0 49 118 69 692.0 0 69 119 50 692.0 0 50 120 45 692.0 0 45 121 55 692.0 0 55 122 69 692.0 0 69 123 66 692.0 0 66 124 212 692.0 0 212 125 133 692.0 0 133 126 325 692.0 0 325 127 94 692.0 0 94 128 62 692.0 0 62 129 53 692.0 0 53 130 75 692.0 0 75 131 238 692.0 0 238 132 570 692.0 0 570 133 189 692.0 0 189 134 93 692.0 0 93 135 56 692.0 0 56 136 62 692.0 0 62 137 45 692.0 0 45 138 48 692.0 0 48 139 61 692.0 0 61 140 70 692.0 0 70 141 63 692.0 0 63 142 63 692.0 0 63 143 59 692.0 0 59 144 82 692.0 0 82 145 99 692.0 0 99 146 98 692.0 0 98 147 102 692.0 0 102 148 122 692.0 0 122 149 60 692.0 0 60 150 70 692.0 0 70 151 65 692.0 0 65 152 77 692.0 0 77 153 74 692.0 0 74 154 99 692.0 0 99 155 112 692.0 0 112 156 91 692.0 0 91 157 69 692.0 0 69 158 63 692.0 0 63 159 59 692.0 0 59 160 69 692.0 0 69 161 71 692.0 0 71 162 80 692.0 0 80 163 72 692.0 0 72 164 73 692.0 0 73 165 88 692.0 0 88 166 90 692.0 0 90 167 112 692.0 0 112 168 90 692.0 0 90 169 71 692.0 0 71 170 77 692.0 0 77 171 88 692.0 0 88 172 101 692.0 0 101 173 102 692.0 0 102 174 193 692.0 0 193 175 205 692.0 0 205 176 174 692.0 0 174 177 81 692.0 0 81 178 92 692.0 0 92 179 188 692.0 0 188 180 140 692.0 0 140 181 210 692.0 0 210 182 121 692.0 0 121 183 88 692.0 0 88 184 96 692.0 0 96 185 273 692.0 0 273 186 187 692.0 0 187 187 311 692.0 0 311 188 130 692.0 0 130 189 126 692.0 0 126 190 134 692.0 0 134 191 155 692.0 0 155 192 287 692.0 0 287 193 1954 692.0 0 1954 194 1052 692.0 0 1052 195 1592 692.0 0 1592 196 141 692.0 0 141 197 136 692.0 0 136 198 101 692.0 0 101 199 345 692.0 0 345 200 392 692.0 0 392 201 601 692.0 0 601 202 194 692.0 0 194 203 83 692.0 0 83 204 87 692.0 0 87 205 126 692.0 0 126 206 158 692.0 0 158 207 144 692.0 0 144 208 130 692.0 0 130 209 109 692.0 0 109 210 127 692.0 0 127 211 168 692.0 0 168 212 139 692.0 0 139 213 255 692.0 0 255 214 153 692.0 0 153 215 195 692.0 0 195 216 172 692.0 0 172 217 187 692.0 0 187 218 204 692.0 0 204 219 360 692.0 0 360 220 313 692.0 0 313 221 415 692.0 0 415 222 621 692.0 0 621 223 888 692.0 0 888 224 1498 692.0 0 1498 225 506 692.0 0 506 226 494 692.0 0 494 227 733 692.0 0 733 228 467 692.0 0 467 229 458 692.0 0 458 230 746 692.0 0 746 231 1026 692.0 0 1026 232 2925 692.0 0 2925 233 2989 692.0 0 2989 234 10537 692.0 0 10537 235 2038 692.0 0 2038 236 1956 692.0 0 1956 237 41417 692.0 0 41417 238 12466 692.0 0 12466 239 48984 692.0 0 48984 240 1524 692.0 0 1524 241 1964 692.0 0 1964 242 819 692.0 0 819 243 1334 692.0 0 1334 244 2208 692.0 0 2208 245 1188 692.0 0 1188 246 936 692.0 0 936 247 1395 692.0 0 1395 248 1031 692.0 0 1031 249 1307 692.0 0 1307 250 2017 692.0 0 2017 251 1948 692.0 0 1948 252 2817 692.0 0 2817 253 2794 692.0 0 2794 254 4343 692.0 0 4343 255 3872 692.0 0 3872 256 4784 692.0 0 4784 257 102436 692.0 0 102436 258 75663 692.0 0 75663 259 230547 692.0 0 230547 260 13200 692.0 0 13200 261 148 692.0 0 148 262 112 692.0 0 112 263 85 692.0 0 85 264 81 692.0 0 81 265 86 692.0 0 86 266 91 692.0 0 91 267 88 692.0 0 88 268 234 692.0 0 234 269 314 692.0 0 314 270 123 692.0 0 123 271 83 692.0 0 83 272 83 692.0 0 83 273 91 692.0 0 91 274 76 692.0 0 76 275 82 692.0 0 82 276 89 692.0 0 89 277 67 692.0 0 67 278 78 692.0 0 78 279 92 692.0 0 92 280 99 692.0 0 99 281 87 692.0 0 87 282 125 692.0 0 125 283 89 692.0 0 89 284 86 692.0 0 86 285 84 692.0 0 84 286 78 692.0 0 78 287 131 692.0 0 131 288 97 692.0 0 97 289 173 692.0 0 173 290 233 692.0 0 233 291 884 692.0 0 884 292 640 692.0 0 640 293 556 692.0 0 556 294 167 692.0 0 167 295 121 692.0 0 121 296 109 692.0 0 109 297 103 692.0 0 103 298 77 692.0 0 77 299 70 692.0 0 70 300 119 692.0 0 119 301 165 692.0 0 165 Finished in 3.34 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 71,347 Reads with adapters: 24,632 (34.5%) Reads that were too short: 70 (0.1%) Reads written (passing filters): 24,562 (34.4%) Total basepairs processed: 18,089,901 bp Total written (filtered): 6,776,484 bp (37.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24632 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 164 0.0 3 0 157 5 2 17 24248 0.0 3 23720 506 20 2 18 68 0.0 3 6 56 2 4 19 27 0.0 3 2 0 21 4 20 30 0.0 3 1 0 0 29 24 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 60 6 0.0 3 0 0 0 6 63 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 5 0.0 3 0 0 0 5 147 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 173 34 0.0 3 0 0 0 34 189 1 0.0 3 1 216 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 1 249 2 0.0 3 1 0 0 1 253 1 0.0 3 0 0 0 1 257 1 0.0 3 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.86 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 24,562 Reads with adapters: 207 (0.8%) Reads written (passing filters): 24,562 (100.0%) Total basepairs processed: 6,776,484 bp Total written (filtered): 6,775,761 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 207 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.1% C: 10.1% G: 44.4% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 107 383.8 0 107 4 99 95.9 0 99 6 1 6.0 0 1