This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 158.72 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 47,846 (1.4%) Reads that were too short: 17,048 (0.5%) Reads written (passing filters): 30,798 (0.9%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 5,595,256 bp (0.5%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 47846 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 27 847.3 0 27 7 24 847.3 0 24 8 18 847.3 0 18 9 9 847.3 0 9 10 11 847.3 0 11 11 16 847.3 0 16 12 17 847.3 0 17 13 43 847.3 0 43 14 15 847.3 0 15 15 18 847.3 0 18 16 27 847.3 0 27 17 59 847.3 0 59 18 311 847.3 0 311 19 18 847.3 0 18 20 16 847.3 0 16 21 24 847.3 0 24 22 35 847.3 0 35 23 59 847.3 0 59 24 16 847.3 0 16 25 32 847.3 0 32 26 370 847.3 0 370 27 124 847.3 0 124 28 279 847.3 0 279 29 240 847.3 0 240 30 263 847.3 0 263 31 285 847.3 0 285 32 8 847.3 0 8 33 22 847.3 0 22 34 14 847.3 0 14 35 24 847.3 0 24 36 26 847.3 0 26 37 13 847.3 0 13 38 19 847.3 0 19 39 18 847.3 0 18 40 17 847.3 0 17 41 11 847.3 0 11 42 29 847.3 0 29 43 13 847.3 0 13 44 15 847.3 0 15 45 20 847.3 0 20 46 16 847.3 0 16 47 16 847.3 0 16 48 20 847.3 0 20 49 20 847.3 0 20 50 14 847.3 0 14 51 31 847.3 0 31 52 39 847.3 0 39 53 16 847.3 0 16 54 35 847.3 0 35 55 18 847.3 0 18 56 29 847.3 0 29 57 49 847.3 0 49 58 34 847.3 0 34 59 34 847.3 0 34 60 28 847.3 0 28 61 28 847.3 0 28 62 38 847.3 0 38 63 43 847.3 0 43 64 48 847.3 0 48 65 40 847.3 0 40 66 56 847.3 0 56 67 70 847.3 0 70 68 91 847.3 0 91 69 471 847.3 0 471 70 2485 847.3 0 2485 71 1189 847.3 0 1189 72 39 847.3 0 39 73 43 847.3 0 43 74 22 847.3 0 22 75 67 847.3 0 67 76 51 847.3 0 51 77 100 847.3 0 100 78 635 847.3 0 635 79 77 847.3 0 77 80 32 847.3 0 32 81 41 847.3 0 41 82 73 847.3 0 73 83 45 847.3 0 45 84 50 847.3 0 50 85 213 847.3 0 213 86 76 847.3 0 76 87 30 847.3 0 30 88 72 847.3 0 72 89 32 847.3 0 32 90 34 847.3 0 34 91 74 847.3 0 74 92 23 847.3 0 23 93 35 847.3 0 35 94 63 847.3 0 63 95 36 847.3 0 36 96 41 847.3 0 41 97 77 847.3 0 77 98 41 847.3 0 41 99 32 847.3 0 32 100 21 847.3 0 21 101 30 847.3 0 30 102 28 847.3 0 28 103 18 847.3 0 18 104 26 847.3 0 26 105 27 847.3 0 27 106 15 847.3 0 15 107 23 847.3 0 23 108 22 847.3 0 22 109 25 847.3 0 25 110 32 847.3 0 32 111 43 847.3 0 43 112 72 847.3 0 72 113 46 847.3 0 46 114 497 847.3 0 497 115 171 847.3 0 171 116 82 847.3 0 82 117 36 847.3 0 36 118 38 847.3 0 38 119 40 847.3 0 40 120 35 847.3 0 35 121 29 847.3 0 29 122 48 847.3 0 48 123 70 847.3 0 70 124 74 847.3 0 74 125 108 847.3 0 108 126 57 847.3 0 57 127 93 847.3 0 93 128 87 847.3 0 87 129 133 847.3 0 133 130 100 847.3 0 100 131 136 847.3 0 136 132 191 847.3 0 191 133 182 847.3 0 182 134 160 847.3 0 160 135 136 847.3 0 136 136 141 847.3 0 141 137 303 847.3 0 303 138 2256 847.3 0 2256 139 1936 847.3 0 1936 140 1752 847.3 0 1752 141 333 847.3 0 333 142 96 847.3 0 96 143 1117 847.3 0 1117 144 958 847.3 0 958 145 544 847.3 0 544 146 65 847.3 0 65 147 107 847.3 0 107 148 739 847.3 0 739 149 1583 847.3 0 1583 150 1521 847.3 0 1521 151 965 847.3 0 965 152 500 847.3 0 500 153 57 847.3 0 57 154 68 847.3 0 68 155 56 847.3 0 56 156 38 847.3 0 38 157 44 847.3 0 44 158 40 847.3 0 40 159 82 847.3 0 82 160 27 847.3 0 27 161 31 847.3 0 31 162 35 847.3 0 35 163 16 847.3 0 16 164 34 847.3 0 34 165 30 847.3 0 30 166 35 847.3 0 35 167 31 847.3 0 31 168 34 847.3 0 34 169 32 847.3 0 32 170 47 847.3 0 47 171 23 847.3 0 23 172 34 847.3 0 34 173 35 847.3 0 35 174 43 847.3 0 43 175 64 847.3 0 64 176 61 847.3 0 61 177 56 847.3 0 56 178 34 847.3 0 34 179 43 847.3 0 43 180 47 847.3 0 47 181 36 847.3 0 36 182 48 847.3 0 48 183 54 847.3 0 54 184 129 847.3 0 129 185 107 847.3 0 107 186 66 847.3 0 66 187 52 847.3 0 52 188 72 847.3 0 72 189 207 847.3 0 207 190 227 847.3 0 227 191 413 847.3 0 413 192 151 847.3 0 151 193 86 847.3 0 86 194 63 847.3 0 63 195 43 847.3 0 43 196 52 847.3 0 52 197 52 847.3 0 52 198 61 847.3 0 61 199 46 847.3 0 46 200 41 847.3 0 41 201 51 847.3 0 51 202 47 847.3 0 47 203 75 847.3 0 75 204 69 847.3 0 69 205 76 847.3 0 76 206 41 847.3 0 41 207 45 847.3 0 45 208 79 847.3 0 79 209 324 847.3 0 324 210 256 847.3 0 256 211 155 847.3 0 155 212 58 847.3 0 58 213 141 847.3 0 141 214 136 847.3 0 136 215 148 847.3 0 148 216 49 847.3 0 49 217 59 847.3 0 59 218 45 847.3 0 45 219 42 847.3 0 42 220 55 847.3 0 55 221 58 847.3 0 58 222 85 847.3 0 85 223 107 847.3 0 107 224 83 847.3 0 83 225 60 847.3 0 60 226 52 847.3 0 52 227 53 847.3 0 53 228 58 847.3 0 58 229 51 847.3 0 51 230 89 847.3 0 89 231 64 847.3 0 64 232 69 847.3 0 69 233 302 847.3 0 302 234 511 847.3 0 511 235 529 847.3 0 529 236 195 847.3 0 195 237 84 847.3 0 84 238 76 847.3 0 76 239 97 847.3 0 97 240 96 847.3 0 96 241 73 847.3 0 73 242 137 847.3 0 137 243 113 847.3 0 113 244 140 847.3 0 140 245 121 847.3 0 121 246 129 847.3 0 129 247 154 847.3 0 154 248 202 847.3 0 202 249 240 847.3 0 240 250 156 847.3 0 156 251 252 847.3 0 252 252 146 847.3 0 146 253 690 847.3 0 690 254 842 847.3 0 842 255 511 847.3 0 511 256 133 847.3 0 133 257 112 847.3 0 112 258 82 847.3 0 82 259 86 847.3 0 86 260 109 847.3 0 109 261 89 847.3 0 89 262 86 847.3 0 86 263 62 847.3 0 62 264 72 847.3 0 72 265 72 847.3 0 72 266 87 847.3 0 87 267 79 847.3 0 79 268 133 847.3 0 133 269 109 847.3 0 109 270 113 847.3 0 113 271 109 847.3 0 109 272 132 847.3 0 132 273 624 847.3 0 624 274 590 847.3 0 590 275 253 847.3 0 253 276 111 847.3 0 111 277 62 847.3 0 62 278 122 847.3 0 122 279 98 847.3 0 98 280 123 847.3 0 123 281 211 847.3 0 211 282 187 847.3 0 187 283 149 847.3 0 149 284 121 847.3 0 121 285 188 847.3 0 188 286 142 847.3 0 142 287 209 847.3 0 209 288 185 847.3 0 185 289 227 847.3 0 227 290 132 847.3 0 132 291 122 847.3 0 122 292 238 847.3 0 238 293 208 847.3 0 208 294 253 847.3 0 253 295 194 847.3 0 194 296 206 847.3 0 206 297 232 847.3 0 232 298 254 847.3 0 254 299 340 847.3 0 340 300 448 847.3 0 448 301 629 847.3 0 629 Finished in 1.83 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 30,798 Reads with adapters: 53 (0.2%) Reads that were too short: 16 (0.1%) Reads written (passing filters): 37 (0.1%) Total basepairs processed: 5,595,256 bp Total written (filtered): 9,086 bp (0.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 2 0.0 4 2 21 20 0.0 4 16 3 0 0 1 22 5 0.0 4 3 0 1 0 1 24 1 0.0 4 0 0 0 1 59 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 84 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 108 5 0.0 4 0 0 0 0 5 127 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 1 163 2 0.0 4 0 0 0 0 2 184 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (270 us/read; 0.22 M reads/minute). === Summary === Total reads processed: 37 Reads with adapters: 4 (10.8%) Reads written (passing filters): 37 (100.0%) Total basepairs processed: 9,086 bp Total written (filtered): 9,045 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 1 10 1 0.0 1 0 1 11 2 0.0 1 0 2