This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 160.22 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 126,444 (3.6%) Reads that were too short: 22,913 (0.7%) Reads written (passing filters): 103,531 (3.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 28,273,662 bp (2.7%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 126444 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4416 847.3 0 4416 7 1343 847.3 0 1343 8 42104 847.3 0 42104 9 34998 847.3 0 34998 10 149 847.3 0 149 11 4 847.3 0 4 12 1 847.3 0 1 13 8 847.3 0 8 14 4 847.3 0 4 15 7 847.3 0 7 16 4 847.3 0 4 17 7 847.3 0 7 18 6 847.3 0 6 19 4 847.3 0 4 20 5 847.3 0 5 21 1 847.3 0 1 22 7 847.3 0 7 23 16 847.3 0 16 24 7 847.3 0 7 25 6 847.3 0 6 26 13 847.3 0 13 27 21 847.3 0 21 28 14 847.3 0 14 29 12 847.3 0 12 30 7 847.3 0 7 31 2 847.3 0 2 32 4 847.3 0 4 33 24 847.3 0 24 34 9 847.3 0 9 35 8 847.3 0 8 36 6 847.3 0 6 37 7 847.3 0 7 38 17 847.3 0 17 39 13 847.3 0 13 40 15 847.3 0 15 41 13 847.3 0 13 42 28 847.3 0 28 43 43 847.3 0 43 44 10 847.3 0 10 45 11 847.3 0 11 46 8 847.3 0 8 47 24 847.3 0 24 48 11 847.3 0 11 49 14 847.3 0 14 50 10 847.3 0 10 51 11 847.3 0 11 52 20 847.3 0 20 53 20 847.3 0 20 54 37 847.3 0 37 55 15 847.3 0 15 56 11 847.3 0 11 57 53 847.3 0 53 58 130 847.3 0 130 59 125 847.3 0 125 60 1069 847.3 0 1069 61 1495 847.3 0 1495 62 2414 847.3 0 2414 63 24 847.3 0 24 64 19 847.3 0 19 65 29 847.3 0 29 66 132 847.3 0 132 67 377 847.3 0 377 68 107 847.3 0 107 69 10 847.3 0 10 70 11 847.3 0 11 71 15 847.3 0 15 72 22 847.3 0 22 73 71 847.3 0 71 74 12 847.3 0 12 75 25 847.3 0 25 76 28 847.3 0 28 77 294 847.3 0 294 78 272 847.3 0 272 79 479 847.3 0 479 80 48 847.3 0 48 81 37 847.3 0 37 82 193 847.3 0 193 83 76 847.3 0 76 84 32 847.3 0 32 85 47 847.3 0 47 86 41 847.3 0 41 87 8 847.3 0 8 88 14 847.3 0 14 89 61 847.3 0 61 90 31 847.3 0 31 91 34 847.3 0 34 92 67 847.3 0 67 93 38 847.3 0 38 94 39 847.3 0 39 95 40 847.3 0 40 96 7 847.3 0 7 97 39 847.3 0 39 98 28 847.3 0 28 99 33 847.3 0 33 100 43 847.3 0 43 101 73 847.3 0 73 102 19 847.3 0 19 103 23 847.3 0 23 104 34 847.3 0 34 105 32 847.3 0 32 106 26 847.3 0 26 107 55 847.3 0 55 108 49 847.3 0 49 109 44 847.3 0 44 110 48 847.3 0 48 111 110 847.3 0 110 112 165 847.3 0 165 113 145 847.3 0 145 114 1317 847.3 0 1317 115 3571 847.3 0 3571 116 700 847.3 0 700 117 76 847.3 0 76 118 29 847.3 0 29 119 32 847.3 0 32 120 21 847.3 0 21 121 24 847.3 0 24 122 39 847.3 0 39 123 30 847.3 0 30 124 39 847.3 0 39 125 56 847.3 0 56 126 32 847.3 0 32 127 47 847.3 0 47 128 28 847.3 0 28 129 41 847.3 0 41 130 48 847.3 0 48 131 56 847.3 0 56 132 60 847.3 0 60 133 43 847.3 0 43 134 23 847.3 0 23 135 33 847.3 0 33 136 50 847.3 0 50 137 47 847.3 0 47 138 61 847.3 0 61 139 45 847.3 0 45 140 34 847.3 0 34 141 43 847.3 0 43 142 40 847.3 0 40 143 78 847.3 0 78 144 72 847.3 0 72 145 73 847.3 0 73 146 47 847.3 0 47 147 56 847.3 0 56 148 46 847.3 0 46 149 75 847.3 0 75 150 23 847.3 0 23 151 22 847.3 0 22 152 29 847.3 0 29 153 34 847.3 0 34 154 40 847.3 0 40 155 41 847.3 0 41 156 29 847.3 0 29 157 33 847.3 0 33 158 32 847.3 0 32 159 37 847.3 0 37 160 32 847.3 0 32 161 41 847.3 0 41 162 38 847.3 0 38 163 68 847.3 0 68 164 79 847.3 0 79 165 309 847.3 0 309 166 193 847.3 0 193 167 141 847.3 0 141 168 42 847.3 0 42 169 34 847.3 0 34 170 36 847.3 0 36 171 39 847.3 0 39 172 37 847.3 0 37 173 51 847.3 0 51 174 34 847.3 0 34 175 32 847.3 0 32 176 34 847.3 0 34 177 50 847.3 0 50 178 47 847.3 0 47 179 59 847.3 0 59 180 85 847.3 0 85 181 273 847.3 0 273 182 37 847.3 0 37 183 32 847.3 0 32 184 74 847.3 0 74 185 104 847.3 0 104 186 61 847.3 0 61 187 53 847.3 0 53 188 58 847.3 0 58 189 108 847.3 0 108 190 130 847.3 0 130 191 215 847.3 0 215 192 355 847.3 0 355 193 104 847.3 0 104 194 26 847.3 0 26 195 58 847.3 0 58 196 56 847.3 0 56 197 78 847.3 0 78 198 92 847.3 0 92 199 54 847.3 0 54 200 54 847.3 0 54 201 49 847.3 0 49 202 45 847.3 0 45 203 144 847.3 0 144 204 591 847.3 0 591 205 140 847.3 0 140 206 42 847.3 0 42 207 105 847.3 0 105 208 144 847.3 0 144 209 86 847.3 0 86 210 89 847.3 0 89 211 52 847.3 0 52 212 90 847.3 0 90 213 133 847.3 0 133 214 226 847.3 0 226 215 85 847.3 0 85 216 118 847.3 0 118 217 133 847.3 0 133 218 132 847.3 0 132 219 108 847.3 0 108 220 38 847.3 0 38 221 31 847.3 0 31 222 38 847.3 0 38 223 45 847.3 0 45 224 94 847.3 0 94 225 121 847.3 0 121 226 139 847.3 0 139 227 68 847.3 0 68 228 64 847.3 0 64 229 72 847.3 0 72 230 54 847.3 0 54 231 81 847.3 0 81 232 144 847.3 0 144 233 116 847.3 0 116 234 103 847.3 0 103 235 149 847.3 0 149 236 343 847.3 0 343 237 494 847.3 0 494 238 628 847.3 0 628 239 844 847.3 0 844 240 106 847.3 0 106 241 109 847.3 0 109 242 159 847.3 0 159 243 190 847.3 0 190 244 327 847.3 0 327 245 5439 847.3 0 5439 246 3236 847.3 0 3236 247 1304 847.3 0 1304 248 86 847.3 0 86 249 45 847.3 0 45 250 63 847.3 0 63 251 64 847.3 0 64 252 55 847.3 0 55 253 60 847.3 0 60 254 121 847.3 0 121 255 110 847.3 0 110 256 67 847.3 0 67 257 58 847.3 0 58 258 59 847.3 0 59 259 130 847.3 0 130 260 123 847.3 0 123 261 117 847.3 0 117 262 185 847.3 0 185 263 134 847.3 0 134 264 174 847.3 0 174 265 390 847.3 0 390 266 329 847.3 0 329 267 196 847.3 0 196 268 46 847.3 0 46 269 32 847.3 0 32 270 199 847.3 0 199 271 223 847.3 0 223 272 219 847.3 0 219 273 69 847.3 0 69 274 42 847.3 0 42 275 47 847.3 0 47 276 84 847.3 0 84 277 134 847.3 0 134 278 142 847.3 0 142 279 138 847.3 0 138 280 86 847.3 0 86 281 50 847.3 0 50 282 53 847.3 0 53 283 68 847.3 0 68 284 82 847.3 0 82 285 122 847.3 0 122 286 118 847.3 0 118 287 91 847.3 0 91 288 69 847.3 0 69 289 69 847.3 0 69 290 59 847.3 0 59 291 85 847.3 0 85 292 97 847.3 0 97 293 102 847.3 0 102 294 93 847.3 0 93 295 110 847.3 0 110 296 67 847.3 0 67 297 121 847.3 0 121 298 118 847.3 0 118 299 107 847.3 0 107 300 116 847.3 0 116 301 90 847.3 0 90 Finished in 7.95 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 103,531 Reads with adapters: 41,143 (39.7%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 41,120 (39.7%) Total basepairs processed: 28,273,662 bp Total written (filtered): 11,115,717 bp (39.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41143 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 5 0.0 3 0 0 0 5 18 31 0.0 3 0 0 19 7 5 19 658 0.0 3 0 588 34 28 8 20 35843 0.0 4 33983 1509 254 80 17 21 193 0.0 4 42 137 9 4 1 22 3521 0.0 4 3323 149 34 9 6 23 139 0.0 4 2 7 2 95 33 24 3 0.0 4 0 0 0 0 3 25 14 0.0 4 0 0 0 10 4 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 0 2 56 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 8 0.0 4 0 0 0 0 8 127 665 0.0 4 0 0 0 0 665 128 26 0.0 4 0 0 0 0 26 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 178 1 0.0 4 1 186 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.54 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 41,120 Reads with adapters: 10,535 (25.6%) Reads written (passing filters): 41,120 (100.0%) Total basepairs processed: 11,115,717 bp Total written (filtered): 11,006,730 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10535 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 5.3% G: 92.0% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 20 642.5 0 20 4 5 160.6 0 5 5 2 40.2 0 2 6 3 10.0 0 3 8 4 0.6 0 4 9 4748 0.2 0 266 4482 10 4162 0.0 1 271 3891 11 1502 0.0 1 40 1462 12 36 0.0 1 2 34 13 7 0.0 1 0 7 14 4 0.0 1 0 4 150 18 0.0 1 16 2 151 1 0.0 1 1 153 4 0.0 1 4 169 1 0.0 1 1 189 1 0.0 1 0 1 208 11 0.0 1 11 209 2 0.0 1 2 210 2 0.0 1 2 223 1 0.0 1 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.