This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 161.73 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 120,047 (3.5%) Reads that were too short: 19,679 (0.6%) Reads written (passing filters): 100,368 (2.9%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 27,946,062 bp (2.7%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 120047 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4948 847.3 0 4948 7 1044 847.3 0 1044 8 39290 847.3 0 39290 9 41000 847.3 0 41000 10 66 847.3 0 66 11 1 847.3 0 1 12 2 847.3 0 2 13 6 847.3 0 6 14 2 847.3 0 2 15 6 847.3 0 6 16 4 847.3 0 4 17 2 847.3 0 2 18 4 847.3 0 4 19 1 847.3 0 1 20 4 847.3 0 4 21 4 847.3 0 4 22 2 847.3 0 2 23 8 847.3 0 8 24 7 847.3 0 7 25 1 847.3 0 1 26 3 847.3 0 3 27 7 847.3 0 7 28 4 847.3 0 4 29 10 847.3 0 10 30 5 847.3 0 5 31 3 847.3 0 3 32 4 847.3 0 4 33 12 847.3 0 12 34 5 847.3 0 5 35 6 847.3 0 6 36 6 847.3 0 6 37 6 847.3 0 6 38 1 847.3 0 1 39 14 847.3 0 14 40 18 847.3 0 18 41 7 847.3 0 7 42 13 847.3 0 13 43 16 847.3 0 16 44 7 847.3 0 7 45 3 847.3 0 3 46 11 847.3 0 11 47 7 847.3 0 7 48 16 847.3 0 16 49 12 847.3 0 12 50 20 847.3 0 20 51 9 847.3 0 9 52 17 847.3 0 17 53 15 847.3 0 15 54 12 847.3 0 12 55 13 847.3 0 13 56 12 847.3 0 12 57 43 847.3 0 43 58 78 847.3 0 78 59 74 847.3 0 74 60 661 847.3 0 661 61 843 847.3 0 843 62 1651 847.3 0 1651 63 15 847.3 0 15 64 20 847.3 0 20 65 17 847.3 0 17 66 69 847.3 0 69 67 371 847.3 0 371 68 70 847.3 0 70 69 6 847.3 0 6 70 7 847.3 0 7 71 8 847.3 0 8 72 7 847.3 0 7 73 37 847.3 0 37 74 6 847.3 0 6 75 9 847.3 0 9 76 11 847.3 0 11 77 157 847.3 0 157 78 176 847.3 0 176 79 395 847.3 0 395 80 14 847.3 0 14 81 25 847.3 0 25 82 32 847.3 0 32 83 20 847.3 0 20 84 29 847.3 0 29 85 25 847.3 0 25 86 45 847.3 0 45 87 9 847.3 0 9 88 17 847.3 0 17 89 29 847.3 0 29 90 33 847.3 0 33 91 21 847.3 0 21 92 19 847.3 0 19 93 41 847.3 0 41 94 49 847.3 0 49 95 28 847.3 0 28 96 23 847.3 0 23 97 44 847.3 0 44 98 37 847.3 0 37 99 38 847.3 0 38 100 28 847.3 0 28 101 55 847.3 0 55 102 16 847.3 0 16 103 29 847.3 0 29 104 32 847.3 0 32 105 28 847.3 0 28 106 23 847.3 0 23 107 54 847.3 0 54 108 49 847.3 0 49 109 30 847.3 0 30 110 37 847.3 0 37 111 79 847.3 0 79 112 124 847.3 0 124 113 86 847.3 0 86 114 639 847.3 0 639 115 2673 847.3 0 2673 116 360 847.3 0 360 117 54 847.3 0 54 118 14 847.3 0 14 119 20 847.3 0 20 120 16 847.3 0 16 121 18 847.3 0 18 122 23 847.3 0 23 123 26 847.3 0 26 124 35 847.3 0 35 125 35 847.3 0 35 126 30 847.3 0 30 127 39 847.3 0 39 128 13 847.3 0 13 129 31 847.3 0 31 130 36 847.3 0 36 131 34 847.3 0 34 132 29 847.3 0 29 133 18 847.3 0 18 134 22 847.3 0 22 135 26 847.3 0 26 136 25 847.3 0 25 137 39 847.3 0 39 138 34 847.3 0 34 139 30 847.3 0 30 140 40 847.3 0 40 141 21 847.3 0 21 142 21 847.3 0 21 143 43 847.3 0 43 144 44 847.3 0 44 145 50 847.3 0 50 146 27 847.3 0 27 147 32 847.3 0 32 148 40 847.3 0 40 149 73 847.3 0 73 150 22 847.3 0 22 151 26 847.3 0 26 152 17 847.3 0 17 153 33 847.3 0 33 154 24 847.3 0 24 155 21 847.3 0 21 156 17 847.3 0 17 157 18 847.3 0 18 158 21 847.3 0 21 159 39 847.3 0 39 160 20 847.3 0 20 161 36 847.3 0 36 162 28 847.3 0 28 163 35 847.3 0 35 164 37 847.3 0 37 165 163 847.3 0 163 166 154 847.3 0 154 167 83 847.3 0 83 168 23 847.3 0 23 169 21 847.3 0 21 170 24 847.3 0 24 171 22 847.3 0 22 172 27 847.3 0 27 173 19 847.3 0 19 174 25 847.3 0 25 175 24 847.3 0 24 176 23 847.3 0 23 177 35 847.3 0 35 178 32 847.3 0 32 179 60 847.3 0 60 180 56 847.3 0 56 181 368 847.3 0 368 182 32 847.3 0 32 183 21 847.3 0 21 184 42 847.3 0 42 185 66 847.3 0 66 186 42 847.3 0 42 187 43 847.3 0 43 188 26 847.3 0 26 189 88 847.3 0 88 190 105 847.3 0 105 191 187 847.3 0 187 192 358 847.3 0 358 193 95 847.3 0 95 194 29 847.3 0 29 195 51 847.3 0 51 196 38 847.3 0 38 197 46 847.3 0 46 198 49 847.3 0 49 199 36 847.3 0 36 200 37 847.3 0 37 201 39 847.3 0 39 202 40 847.3 0 40 203 133 847.3 0 133 204 681 847.3 0 681 205 141 847.3 0 141 206 29 847.3 0 29 207 81 847.3 0 81 208 87 847.3 0 87 209 51 847.3 0 51 210 80 847.3 0 80 211 29 847.3 0 29 212 77 847.3 0 77 213 215 847.3 0 215 214 386 847.3 0 386 215 52 847.3 0 52 216 60 847.3 0 60 217 67 847.3 0 67 218 62 847.3 0 62 219 49 847.3 0 49 220 30 847.3 0 30 221 31 847.3 0 31 222 23 847.3 0 23 223 36 847.3 0 36 224 36 847.3 0 36 225 46 847.3 0 46 226 55 847.3 0 55 227 44 847.3 0 44 228 49 847.3 0 49 229 36 847.3 0 36 230 39 847.3 0 39 231 68 847.3 0 68 232 92 847.3 0 92 233 70 847.3 0 70 234 89 847.3 0 89 235 103 847.3 0 103 236 141 847.3 0 141 237 285 847.3 0 285 238 350 847.3 0 350 239 412 847.3 0 412 240 79 847.3 0 79 241 81 847.3 0 81 242 106 847.3 0 106 243 164 847.3 0 164 244 270 847.3 0 270 245 5798 847.3 0 5798 246 3574 847.3 0 3574 247 1253 847.3 0 1253 248 49 847.3 0 49 249 34 847.3 0 34 250 39 847.3 0 39 251 42 847.3 0 42 252 42 847.3 0 42 253 47 847.3 0 47 254 65 847.3 0 65 255 82 847.3 0 82 256 54 847.3 0 54 257 26 847.3 0 26 258 28 847.3 0 28 259 62 847.3 0 62 260 89 847.3 0 89 261 108 847.3 0 108 262 163 847.3 0 163 263 72 847.3 0 72 264 88 847.3 0 88 265 451 847.3 0 451 266 304 847.3 0 304 267 208 847.3 0 208 268 35 847.3 0 35 269 20 847.3 0 20 270 81 847.3 0 81 271 112 847.3 0 112 272 114 847.3 0 114 273 35 847.3 0 35 274 23 847.3 0 23 275 39 847.3 0 39 276 58 847.3 0 58 277 94 847.3 0 94 278 81 847.3 0 81 279 55 847.3 0 55 280 35 847.3 0 35 281 26 847.3 0 26 282 39 847.3 0 39 283 37 847.3 0 37 284 41 847.3 0 41 285 76 847.3 0 76 286 68 847.3 0 68 287 42 847.3 0 42 288 44 847.3 0 44 289 44 847.3 0 44 290 36 847.3 0 36 291 56 847.3 0 56 292 88 847.3 0 88 293 52 847.3 0 52 294 58 847.3 0 58 295 76 847.3 0 76 296 55 847.3 0 55 297 80 847.3 0 80 298 59 847.3 0 59 299 65 847.3 0 65 300 71 847.3 0 71 301 51 847.3 0 51 Finished in 7.17 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 100,368 Reads with adapters: 39,166 (39.0%) Reads that were too short: 137 (0.1%) Reads written (passing filters): 39,029 (38.9%) Total basepairs processed: 27,946,062 bp Total written (filtered): 10,768,342 bp (38.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 39166 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 15 0.0 3 0 0 14 1 16 308 0.0 3 0 299 8 1 17 38539 0.0 3 37807 712 17 3 18 92 0.0 3 23 65 3 1 19 21 0.0 3 2 0 17 2 20 20 0.0 3 0 0 1 19 21 1 0.0 3 0 0 0 1 22 1 0.0 3 1 26 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 41 1 0.0 3 1 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 5 0.0 3 0 0 0 5 81 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 148 4 0.0 3 0 0 0 4 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 3 0.0 3 0 0 0 3 169 2 0.0 3 0 0 0 2 207 5 0.0 3 0 0 0 5 215 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 102 0.0 3 0 0 1 101 218 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.31 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 39,029 Reads with adapters: 734 (1.9%) Reads written (passing filters): 39,029 (100.0%) Total basepairs processed: 10,768,342 bp Total written (filtered): 10,765,740 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 734 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.1% C: 2.7% G: 53.4% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 340 609.8 0 340 4 389 152.5 0 389 5 4 38.1 0 4 6 1 9.5 0 1