This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 164.42 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 275,364 (7.9%) Reads that were too short: 60,891 (1.8%) Reads written (passing filters): 214,473 (6.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 59,445,977 bp (5.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 275364 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1426 847.3 0 1426 7 85656 847.3 0 85656 8 97337 847.3 0 97337 9 153 847.3 0 153 10 84 847.3 0 84 11 81 847.3 0 81 12 63 847.3 0 63 13 75 847.3 0 75 14 76 847.3 0 76 15 66 847.3 0 66 16 79 847.3 0 79 17 90 847.3 0 90 18 61 847.3 0 61 19 67 847.3 0 67 20 85 847.3 0 85 21 64 847.3 0 64 22 57 847.3 0 57 23 79 847.3 0 79 24 74 847.3 0 74 25 107 847.3 0 107 26 90 847.3 0 90 27 58 847.3 0 58 28 62 847.3 0 62 29 60 847.3 0 60 30 71 847.3 0 71 31 89 847.3 0 89 32 99 847.3 0 99 33 48 847.3 0 48 34 87 847.3 0 87 35 87 847.3 0 87 36 71 847.3 0 71 37 75 847.3 0 75 38 95 847.3 0 95 39 70 847.3 0 70 40 71 847.3 0 71 41 81 847.3 0 81 42 61 847.3 0 61 43 88 847.3 0 88 44 77 847.3 0 77 45 78 847.3 0 78 46 68 847.3 0 68 47 73 847.3 0 73 48 83 847.3 0 83 49 58 847.3 0 58 50 77 847.3 0 77 51 76 847.3 0 76 52 109 847.3 0 109 53 102 847.3 0 102 54 72 847.3 0 72 55 65 847.3 0 65 56 77 847.3 0 77 57 84 847.3 0 84 58 79 847.3 0 79 59 126 847.3 0 126 60 255 847.3 0 255 61 178 847.3 0 178 62 75 847.3 0 75 63 97 847.3 0 97 64 88 847.3 0 88 65 111 847.3 0 111 66 121 847.3 0 121 67 80 847.3 0 80 68 74 847.3 0 74 69 94 847.3 0 94 70 103 847.3 0 103 71 95 847.3 0 95 72 148 847.3 0 148 73 216 847.3 0 216 74 274 847.3 0 274 75 144 847.3 0 144 76 101 847.3 0 101 77 105 847.3 0 105 78 177 847.3 0 177 79 210 847.3 0 210 80 213 847.3 0 213 81 205 847.3 0 205 82 225 847.3 0 225 83 172 847.3 0 172 84 216 847.3 0 216 85 212 847.3 0 212 86 301 847.3 0 301 87 188 847.3 0 188 88 151 847.3 0 151 89 118 847.3 0 118 90 121 847.3 0 121 91 113 847.3 0 113 92 101 847.3 0 101 93 121 847.3 0 121 94 103 847.3 0 103 95 120 847.3 0 120 96 120 847.3 0 120 97 83 847.3 0 83 98 97 847.3 0 97 99 129 847.3 0 129 100 105 847.3 0 105 101 107 847.3 0 107 102 134 847.3 0 134 103 179 847.3 0 179 104 190 847.3 0 190 105 149 847.3 0 149 106 149 847.3 0 149 107 173 847.3 0 173 108 128 847.3 0 128 109 152 847.3 0 152 110 145 847.3 0 145 111 151 847.3 0 151 112 134 847.3 0 134 113 162 847.3 0 162 114 125 847.3 0 125 115 160 847.3 0 160 116 191 847.3 0 191 117 158 847.3 0 158 118 133 847.3 0 133 119 159 847.3 0 159 120 144 847.3 0 144 121 159 847.3 0 159 122 172 847.3 0 172 123 164 847.3 0 164 124 283 847.3 0 283 125 254 847.3 0 254 126 244 847.3 0 244 127 170 847.3 0 170 128 149 847.3 0 149 129 151 847.3 0 151 130 165 847.3 0 165 131 186 847.3 0 186 132 179 847.3 0 179 133 205 847.3 0 205 134 239 847.3 0 239 135 207 847.3 0 207 136 145 847.3 0 145 137 175 847.3 0 175 138 157 847.3 0 157 139 134 847.3 0 134 140 132 847.3 0 132 141 173 847.3 0 173 142 133 847.3 0 133 143 195 847.3 0 195 144 162 847.3 0 162 145 168 847.3 0 168 146 174 847.3 0 174 147 240 847.3 0 240 148 212 847.3 0 212 149 334 847.3 0 334 150 207 847.3 0 207 151 184 847.3 0 184 152 161 847.3 0 161 153 157 847.3 0 157 154 167 847.3 0 167 155 169 847.3 0 169 156 195 847.3 0 195 157 187 847.3 0 187 158 310 847.3 0 310 159 228 847.3 0 228 160 250 847.3 0 250 161 190 847.3 0 190 162 201 847.3 0 201 163 167 847.3 0 167 164 176 847.3 0 176 165 181 847.3 0 181 166 199 847.3 0 199 167 223 847.3 0 223 168 178 847.3 0 178 169 336 847.3 0 336 170 383 847.3 0 383 171 480 847.3 0 480 172 403 847.3 0 403 173 203 847.3 0 203 174 199 847.3 0 199 175 194 847.3 0 194 176 162 847.3 0 162 177 145 847.3 0 145 178 177 847.3 0 177 179 216 847.3 0 216 180 217 847.3 0 217 181 221 847.3 0 221 182 189 847.3 0 189 183 195 847.3 0 195 184 221 847.3 0 221 185 233 847.3 0 233 186 173 847.3 0 173 187 341 847.3 0 341 188 356 847.3 0 356 189 277 847.3 0 277 190 225 847.3 0 225 191 187 847.3 0 187 192 230 847.3 0 230 193 217 847.3 0 217 194 218 847.3 0 218 195 214 847.3 0 214 196 188 847.3 0 188 197 161 847.3 0 161 198 175 847.3 0 175 199 233 847.3 0 233 200 266 847.3 0 266 201 217 847.3 0 217 202 193 847.3 0 193 203 280 847.3 0 280 204 310 847.3 0 310 205 331 847.3 0 331 206 168 847.3 0 168 207 161 847.3 0 161 208 140 847.3 0 140 209 168 847.3 0 168 210 157 847.3 0 157 211 194 847.3 0 194 212 185 847.3 0 185 213 219 847.3 0 219 214 574 847.3 0 574 215 1012 847.3 0 1012 216 1351 847.3 0 1351 217 1402 847.3 0 1402 218 275 847.3 0 275 219 297 847.3 0 297 220 213 847.3 0 213 221 192 847.3 0 192 222 226 847.3 0 226 223 259 847.3 0 259 224 353 847.3 0 353 225 541 847.3 0 541 226 1923 847.3 0 1923 227 3872 847.3 0 3872 228 4840 847.3 0 4840 229 8604 847.3 0 8604 230 1305 847.3 0 1305 231 1999 847.3 0 1999 232 1763 847.3 0 1763 233 3308 847.3 0 3308 234 196 847.3 0 196 235 136 847.3 0 136 236 156 847.3 0 156 237 179 847.3 0 179 238 208 847.3 0 208 239 257 847.3 0 257 240 203 847.3 0 203 241 168 847.3 0 168 242 146 847.3 0 146 243 156 847.3 0 156 244 172 847.3 0 172 245 176 847.3 0 176 246 134 847.3 0 134 247 218 847.3 0 218 248 294 847.3 0 294 249 313 847.3 0 313 250 282 847.3 0 282 251 180 847.3 0 180 252 164 847.3 0 164 253 152 847.3 0 152 254 213 847.3 0 213 255 229 847.3 0 229 256 196 847.3 0 196 257 218 847.3 0 218 258 247 847.3 0 247 259 263 847.3 0 263 260 287 847.3 0 287 261 275 847.3 0 275 262 237 847.3 0 237 263 272 847.3 0 272 264 672 847.3 0 672 265 1268 847.3 0 1268 266 2113 847.3 0 2113 267 1791 847.3 0 1791 268 2228 847.3 0 2228 269 355 847.3 0 355 270 215 847.3 0 215 271 220 847.3 0 220 272 218 847.3 0 218 273 317 847.3 0 317 274 362 847.3 0 362 275 361 847.3 0 361 276 422 847.3 0 422 277 271 847.3 0 271 278 217 847.3 0 217 279 268 847.3 0 268 280 299 847.3 0 299 281 247 847.3 0 247 282 216 847.3 0 216 283 197 847.3 0 197 284 210 847.3 0 210 285 218 847.3 0 218 286 287 847.3 0 287 287 261 847.3 0 261 288 219 847.3 0 219 289 189 847.3 0 189 290 198 847.3 0 198 291 1026 847.3 0 1026 292 803 847.3 0 803 293 644 847.3 0 644 294 219 847.3 0 219 295 153 847.3 0 153 296 140 847.3 0 140 297 113 847.3 0 113 298 153 847.3 0 153 299 172 847.3 0 172 300 219 847.3 0 219 301 268 847.3 0 268 Finished in 15.32 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 214,473 Reads with adapters: 84,817 (39.5%) Reads that were too short: 97 (0.0%) Reads written (passing filters): 84,720 (39.5%) Total basepairs processed: 59,445,977 bp Total written (filtered): 23,461,639 bp (39.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 84817 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 25 0.0 3 0 0 22 3 16 544 0.0 3 0 530 14 17 83815 0.0 3 82222 1553 34 6 18 182 0.0 3 23 155 4 19 42 0.0 3 1 0 34 7 20 69 0.0 3 0 0 0 69 21 1 0.0 3 0 1 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 25 3 0.0 3 1 0 0 2 33 2 0.0 3 0 0 1 1 37 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 60 1 0.0 3 1 67 9 0.0 3 0 0 1 8 68 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 1 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 49 0.0 3 0 0 0 49 234 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 0 5 282 2 0.0 3 0 0 1 1 283 5 0.0 3 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.96 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 84,720 Reads with adapters: 286 (0.3%) Reads written (passing filters): 84,720 (100.0%) Total basepairs processed: 23,461,639 bp Total written (filtered): 23,460,685 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 286 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.1% C: 11.2% G: 43.7% T: 29.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 208 1323.8 0 208 4 67 330.9 0 67 5 9 82.7 0 9 6 1 20.7 0 1 11 1 0.0 1 0 1