This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 158.19 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 71,081 (2.0%) Reads that were too short: 12,037 (0.3%) Reads written (passing filters): 59,044 (1.7%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 13,060,958 bp (1.3%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 71081 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 580 847.3 0 580 7 10311 847.3 0 10311 8 255 847.3 0 255 9 10714 847.3 0 10714 10 12 847.3 0 12 11 7 847.3 0 7 12 2 847.3 0 2 13 2 847.3 0 2 14 1 847.3 0 1 15 3 847.3 0 3 16 2 847.3 0 2 17 1 847.3 0 1 19 5 847.3 0 5 20 5 847.3 0 5 21 2 847.3 0 2 22 15 847.3 0 15 23 18 847.3 0 18 24 3 847.3 0 3 25 45 847.3 0 45 26 19 847.3 0 19 27 10 847.3 0 10 28 6 847.3 0 6 29 6 847.3 0 6 30 10 847.3 0 10 31 3 847.3 0 3 32 13 847.3 0 13 33 27 847.3 0 27 34 630 847.3 0 630 35 388 847.3 0 388 36 11 847.3 0 11 37 6 847.3 0 6 38 4 847.3 0 4 39 12 847.3 0 12 40 14 847.3 0 14 41 11 847.3 0 11 42 3 847.3 0 3 43 4 847.3 0 4 44 5 847.3 0 5 45 2 847.3 0 2 46 3 847.3 0 3 47 2 847.3 0 2 48 2 847.3 0 2 49 1 847.3 0 1 50 5 847.3 0 5 51 2 847.3 0 2 52 8 847.3 0 8 53 9 847.3 0 9 54 5 847.3 0 5 55 3 847.3 0 3 56 6 847.3 0 6 57 4 847.3 0 4 58 6 847.3 0 6 59 7 847.3 0 7 60 9 847.3 0 9 61 5 847.3 0 5 62 3 847.3 0 3 63 5 847.3 0 5 64 4 847.3 0 4 65 4 847.3 0 4 66 8 847.3 0 8 67 8 847.3 0 8 68 8 847.3 0 8 69 3 847.3 0 3 70 9 847.3 0 9 71 4 847.3 0 4 72 8 847.3 0 8 73 10 847.3 0 10 74 115 847.3 0 115 75 41 847.3 0 41 76 42 847.3 0 42 77 50 847.3 0 50 78 50 847.3 0 50 79 31 847.3 0 31 80 19 847.3 0 19 81 36 847.3 0 36 82 39 847.3 0 39 83 31 847.3 0 31 84 18 847.3 0 18 85 24 847.3 0 24 86 41 847.3 0 41 87 45 847.3 0 45 88 44 847.3 0 44 89 59 847.3 0 59 90 49 847.3 0 49 91 41 847.3 0 41 92 35 847.3 0 35 93 161 847.3 0 161 94 95 847.3 0 95 95 89 847.3 0 89 96 85 847.3 0 85 97 202 847.3 0 202 98 234 847.3 0 234 99 1328 847.3 0 1328 100 798 847.3 0 798 101 554 847.3 0 554 102 75 847.3 0 75 103 65 847.3 0 65 104 77 847.3 0 77 105 57 847.3 0 57 106 72 847.3 0 72 107 81 847.3 0 81 108 79 847.3 0 79 109 152 847.3 0 152 110 276 847.3 0 276 111 3116 847.3 0 3116 112 3316 847.3 0 3316 113 3985 847.3 0 3985 114 2421 847.3 0 2421 115 1704 847.3 0 1704 116 40 847.3 0 40 117 32 847.3 0 32 118 41 847.3 0 41 119 66 847.3 0 66 120 30 847.3 0 30 121 41 847.3 0 41 122 33 847.3 0 33 123 32 847.3 0 32 124 20 847.3 0 20 125 63 847.3 0 63 126 45 847.3 0 45 127 88 847.3 0 88 128 91 847.3 0 91 129 865 847.3 0 865 130 320 847.3 0 320 131 225 847.3 0 225 132 104 847.3 0 104 133 99 847.3 0 99 134 69 847.3 0 69 135 181 847.3 0 181 136 189 847.3 0 189 137 166 847.3 0 166 138 111 847.3 0 111 139 106 847.3 0 106 140 126 847.3 0 126 141 182 847.3 0 182 142 1522 847.3 0 1522 143 4230 847.3 0 4230 144 2732 847.3 0 2732 145 1145 847.3 0 1145 146 78 847.3 0 78 147 65 847.3 0 65 148 49 847.3 0 49 149 46 847.3 0 46 150 29 847.3 0 29 151 31 847.3 0 31 152 43 847.3 0 43 153 39 847.3 0 39 154 38 847.3 0 38 155 42 847.3 0 42 156 49 847.3 0 49 157 59 847.3 0 59 158 88 847.3 0 88 159 49 847.3 0 49 160 62 847.3 0 62 161 57 847.3 0 57 162 77 847.3 0 77 163 34 847.3 0 34 164 33 847.3 0 33 165 46 847.3 0 46 166 60 847.3 0 60 167 30 847.3 0 30 168 41 847.3 0 41 169 40 847.3 0 40 170 44 847.3 0 44 171 36 847.3 0 36 172 33 847.3 0 33 173 30 847.3 0 30 174 38 847.3 0 38 175 33 847.3 0 33 176 44 847.3 0 44 177 67 847.3 0 67 178 65 847.3 0 65 179 53 847.3 0 53 180 53 847.3 0 53 181 51 847.3 0 51 182 55 847.3 0 55 183 50 847.3 0 50 184 41 847.3 0 41 185 48 847.3 0 48 186 44 847.3 0 44 187 70 847.3 0 70 188 85 847.3 0 85 189 80 847.3 0 80 190 107 847.3 0 107 191 52 847.3 0 52 192 44 847.3 0 44 193 45 847.3 0 45 194 45 847.3 0 45 195 73 847.3 0 73 196 55 847.3 0 55 197 70 847.3 0 70 198 48 847.3 0 48 199 45 847.3 0 45 200 55 847.3 0 55 201 56 847.3 0 56 202 64 847.3 0 64 203 81 847.3 0 81 204 63 847.3 0 63 205 43 847.3 0 43 206 49 847.3 0 49 207 66 847.3 0 66 208 67 847.3 0 67 209 58 847.3 0 58 210 65 847.3 0 65 211 60 847.3 0 60 212 42 847.3 0 42 213 59 847.3 0 59 214 57 847.3 0 57 215 49 847.3 0 49 216 56 847.3 0 56 217 58 847.3 0 58 218 55 847.3 0 55 219 47 847.3 0 47 220 75 847.3 0 75 221 90 847.3 0 90 222 91 847.3 0 91 223 116 847.3 0 116 224 157 847.3 0 157 225 77 847.3 0 77 226 356 847.3 0 356 227 36 847.3 0 36 228 33 847.3 0 33 229 72 847.3 0 72 230 60 847.3 0 60 231 53 847.3 0 53 232 63 847.3 0 63 233 83 847.3 0 83 234 91 847.3 0 91 235 95 847.3 0 95 236 86 847.3 0 86 237 75 847.3 0 75 238 102 847.3 0 102 239 89 847.3 0 89 240 103 847.3 0 103 241 143 847.3 0 143 242 127 847.3 0 127 243 149 847.3 0 149 244 102 847.3 0 102 245 82 847.3 0 82 246 70 847.3 0 70 247 61 847.3 0 61 248 76 847.3 0 76 249 60 847.3 0 60 250 80 847.3 0 80 251 79 847.3 0 79 252 117 847.3 0 117 253 96 847.3 0 96 254 100 847.3 0 100 255 97 847.3 0 97 256 122 847.3 0 122 257 198 847.3 0 198 258 177 847.3 0 177 259 138 847.3 0 138 260 93 847.3 0 93 261 92 847.3 0 92 262 174 847.3 0 174 263 209 847.3 0 209 264 232 847.3 0 232 265 86 847.3 0 86 266 61 847.3 0 61 267 53 847.3 0 53 268 63 847.3 0 63 269 65 847.3 0 65 270 103 847.3 0 103 271 116 847.3 0 116 272 93 847.3 0 93 273 78 847.3 0 78 274 109 847.3 0 109 275 198 847.3 0 198 276 146 847.3 0 146 277 182 847.3 0 182 278 125 847.3 0 125 279 80 847.3 0 80 280 103 847.3 0 103 281 104 847.3 0 104 282 153 847.3 0 153 283 241 847.3 0 241 284 358 847.3 0 358 285 201 847.3 0 201 286 90 847.3 0 90 287 84 847.3 0 84 288 66 847.3 0 66 289 99 847.3 0 99 290 541 847.3 0 541 291 788 847.3 0 788 292 277 847.3 0 277 293 192 847.3 0 192 294 120 847.3 0 120 295 390 847.3 0 390 296 142 847.3 0 142 297 103 847.3 0 103 298 150 847.3 0 150 299 133 847.3 0 133 300 128 847.3 0 128 301 100 847.3 0 100 Finished in 3.97 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 59,044 Reads with adapters: 10,872 (18.4%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 10,824 (18.3%) Total basepairs processed: 13,060,958 bp Total written (filtered): 2,949,477 bp (22.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10872 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 1 19 137 0.0 3 0 113 16 6 2 20 10432 0.0 4 9935 408 69 12 8 21 64 0.0 4 40 23 1 22 14 0.0 4 8 1 1 0 4 23 18 0.0 4 4 1 1 10 2 28 3 0.0 4 0 0 0 0 3 35 13 0.0 4 0 0 0 0 13 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 122 11 0.0 4 0 0 0 0 11 123 7 0.0 4 0 0 0 1 6 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 133 0.0 4 0 0 0 0 133 128 8 0.0 4 0 0 0 1 7 142 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 201 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 10,824 Reads with adapters: 696 (6.4%) Reads written (passing filters): 10,824 (100.0%) Total basepairs processed: 2,949,477 bp Total written (filtered): 2,940,482 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 696 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.6% C: 8.2% G: 83.6% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 35 169.1 0 35 4 6 42.3 0 6 5 1 10.6 0 1 9 4 0.0 0 0 4 10 10 0.0 1 2 8 11 538 0.0 1 18 520 12 83 0.0 1 4 79 13 4 0.0 1 0 4 14 1 0.0 1 0 1 20 4 0.0 1 4 24 1 0.0 1 1 61 1 0.0 1 1 152 2 0.0 1 2 210 4 0.0 1 4 211 1 0.0 1 1 225 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.