This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 165.47 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 260,209 (7.5%) Reads that were too short: 9,932 (0.3%) Reads written (passing filters): 250,277 (7.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 70,751,622 bp (6.8%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 260209 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2115 847.3 0 2115 7 111163 847.3 0 111163 8 2892 847.3 0 2892 9 112075 847.3 0 112075 10 104 847.3 0 104 11 38 847.3 0 38 12 38 847.3 0 38 13 42 847.3 0 42 14 27 847.3 0 27 15 13 847.3 0 13 16 44 847.3 0 44 17 51 847.3 0 51 18 27 847.3 0 27 19 24 847.3 0 24 20 24 847.3 0 24 21 17 847.3 0 17 22 25 847.3 0 25 23 20 847.3 0 20 24 25 847.3 0 25 25 34 847.3 0 34 26 23 847.3 0 23 27 16 847.3 0 16 28 31 847.3 0 31 29 40 847.3 0 40 30 40 847.3 0 40 31 27 847.3 0 27 32 28 847.3 0 28 33 53 847.3 0 53 34 31 847.3 0 31 35 26 847.3 0 26 36 32 847.3 0 32 37 27 847.3 0 27 38 34 847.3 0 34 39 29 847.3 0 29 40 55 847.3 0 55 41 44 847.3 0 44 42 47 847.3 0 47 43 40 847.3 0 40 44 95 847.3 0 95 45 111 847.3 0 111 46 285 847.3 0 285 47 36 847.3 0 36 48 29 847.3 0 29 49 29 847.3 0 29 50 36 847.3 0 36 51 43 847.3 0 43 52 35 847.3 0 35 53 34 847.3 0 34 54 30 847.3 0 30 55 27 847.3 0 27 56 26 847.3 0 26 57 33 847.3 0 33 58 196 847.3 0 196 59 89 847.3 0 89 60 52 847.3 0 52 61 31 847.3 0 31 62 34 847.3 0 34 63 20 847.3 0 20 64 30 847.3 0 30 65 34 847.3 0 34 66 31 847.3 0 31 67 39 847.3 0 39 68 34 847.3 0 34 69 30 847.3 0 30 70 20 847.3 0 20 71 16 847.3 0 16 72 22 847.3 0 22 73 30 847.3 0 30 74 29 847.3 0 29 75 47 847.3 0 47 76 43 847.3 0 43 77 46 847.3 0 46 78 29 847.3 0 29 79 42 847.3 0 42 80 42 847.3 0 42 81 49 847.3 0 49 82 36 847.3 0 36 83 55 847.3 0 55 84 51 847.3 0 51 85 40 847.3 0 40 86 36 847.3 0 36 87 49 847.3 0 49 88 53 847.3 0 53 89 72 847.3 0 72 90 64 847.3 0 64 91 73 847.3 0 73 92 81 847.3 0 81 93 59 847.3 0 59 94 70 847.3 0 70 95 54 847.3 0 54 96 72 847.3 0 72 97 69 847.3 0 69 98 53 847.3 0 53 99 66 847.3 0 66 100 55 847.3 0 55 101 73 847.3 0 73 102 58 847.3 0 58 103 71 847.3 0 71 104 79 847.3 0 79 105 75 847.3 0 75 106 638 847.3 0 638 107 1036 847.3 0 1036 108 859 847.3 0 859 109 72 847.3 0 72 110 64 847.3 0 64 111 59 847.3 0 59 112 62 847.3 0 62 113 62 847.3 0 62 114 638 847.3 0 638 115 466 847.3 0 466 116 352 847.3 0 352 117 41 847.3 0 41 118 39 847.3 0 39 119 57 847.3 0 57 120 53 847.3 0 53 121 49 847.3 0 49 122 41 847.3 0 41 123 71 847.3 0 71 124 67 847.3 0 67 125 58 847.3 0 58 126 63 847.3 0 63 127 81 847.3 0 81 128 193 847.3 0 193 129 189 847.3 0 189 130 81 847.3 0 81 131 51 847.3 0 51 132 71 847.3 0 71 133 114 847.3 0 114 134 186 847.3 0 186 135 206 847.3 0 206 136 400 847.3 0 400 137 204 847.3 0 204 138 195 847.3 0 195 139 201 847.3 0 201 140 407 847.3 0 407 141 464 847.3 0 464 142 115 847.3 0 115 143 87 847.3 0 87 144 93 847.3 0 93 145 85 847.3 0 85 146 91 847.3 0 91 147 112 847.3 0 112 148 2274 847.3 0 2274 149 1134 847.3 0 1134 150 1344 847.3 0 1344 151 49 847.3 0 49 152 50 847.3 0 50 153 49 847.3 0 49 154 51 847.3 0 51 155 126 847.3 0 126 156 302 847.3 0 302 157 185 847.3 0 185 158 74 847.3 0 74 159 52 847.3 0 52 160 44 847.3 0 44 161 68 847.3 0 68 162 160 847.3 0 160 163 235 847.3 0 235 164 417 847.3 0 417 165 373 847.3 0 373 166 50 847.3 0 50 167 49 847.3 0 49 168 58 847.3 0 58 169 45 847.3 0 45 170 32 847.3 0 32 171 25 847.3 0 25 172 32 847.3 0 32 173 34 847.3 0 34 174 34 847.3 0 34 175 35 847.3 0 35 176 53 847.3 0 53 177 38 847.3 0 38 178 59 847.3 0 59 179 72 847.3 0 72 180 134 847.3 0 134 181 121 847.3 0 121 182 104 847.3 0 104 183 108 847.3 0 108 184 45 847.3 0 45 185 61 847.3 0 61 186 60 847.3 0 60 187 45 847.3 0 45 188 40 847.3 0 40 189 58 847.3 0 58 190 61 847.3 0 61 191 57 847.3 0 57 192 73 847.3 0 73 193 58 847.3 0 58 194 46 847.3 0 46 195 45 847.3 0 45 196 45 847.3 0 45 197 41 847.3 0 41 198 57 847.3 0 57 199 58 847.3 0 58 200 57 847.3 0 57 201 58 847.3 0 58 202 68 847.3 0 68 203 143 847.3 0 143 204 137 847.3 0 137 205 48 847.3 0 48 206 47 847.3 0 47 207 35 847.3 0 35 208 42 847.3 0 42 209 87 847.3 0 87 210 47 847.3 0 47 211 38 847.3 0 38 212 42 847.3 0 42 213 54 847.3 0 54 214 82 847.3 0 82 215 77 847.3 0 77 216 61 847.3 0 61 217 67 847.3 0 67 218 42 847.3 0 42 219 38 847.3 0 38 220 50 847.3 0 50 221 46 847.3 0 46 222 36 847.3 0 36 223 63 847.3 0 63 224 61 847.3 0 61 225 68 847.3 0 68 226 59 847.3 0 59 227 45 847.3 0 45 228 65 847.3 0 65 229 56 847.3 0 56 230 48 847.3 0 48 231 63 847.3 0 63 232 83 847.3 0 83 233 119 847.3 0 119 234 105 847.3 0 105 235 64 847.3 0 64 236 87 847.3 0 87 237 57 847.3 0 57 238 75 847.3 0 75 239 50 847.3 0 50 240 54 847.3 0 54 241 50 847.3 0 50 242 80 847.3 0 80 243 68 847.3 0 68 244 85 847.3 0 85 245 58 847.3 0 58 246 60 847.3 0 60 247 63 847.3 0 63 248 74 847.3 0 74 249 41 847.3 0 41 250 76 847.3 0 76 251 69 847.3 0 69 252 80 847.3 0 80 253 126 847.3 0 126 254 88 847.3 0 88 255 80 847.3 0 80 256 67 847.3 0 67 257 67 847.3 0 67 258 64 847.3 0 64 259 69 847.3 0 69 260 89 847.3 0 89 261 140 847.3 0 140 262 126 847.3 0 126 263 77 847.3 0 77 264 76 847.3 0 76 265 73 847.3 0 73 266 69 847.3 0 69 267 73 847.3 0 73 268 79 847.3 0 79 269 87 847.3 0 87 270 137 847.3 0 137 271 590 847.3 0 590 272 402 847.3 0 402 273 179 847.3 0 179 274 227 847.3 0 227 275 370 847.3 0 370 276 68 847.3 0 68 277 91 847.3 0 91 278 107 847.3 0 107 279 104 847.3 0 104 280 91 847.3 0 91 281 81 847.3 0 81 282 69 847.3 0 69 283 95 847.3 0 95 284 91 847.3 0 91 285 102 847.3 0 102 286 71 847.3 0 71 287 75 847.3 0 75 288 70 847.3 0 70 289 66 847.3 0 66 290 85 847.3 0 85 291 124 847.3 0 124 292 124 847.3 0 124 293 94 847.3 0 94 294 159 847.3 0 159 295 302 847.3 0 302 296 381 847.3 0 381 297 395 847.3 0 395 298 227 847.3 0 227 299 58 847.3 0 58 300 79 847.3 0 79 301 55 847.3 0 55 Finished in 18.14 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 250,277 Reads with adapters: 111,343 (44.5%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 111,311 (44.5%) Total basepairs processed: 70,751,622 bp Total written (filtered): 30,820,308 bp (43.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 111343 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 21 0.0 3 0 0 20 1 16 684 0.0 3 0 673 10 1 17 110031 0.0 3 108082 1903 39 7 18 269 0.0 3 28 237 4 19 53 0.0 3 5 0 43 5 20 159 0.0 3 0 0 0 159 22 2 0.0 3 2 23 2 0.0 3 2 24 1 0.0 3 1 27 2 0.0 3 0 0 0 2 28 3 0.0 3 0 0 0 3 29 1 0.0 3 1 30 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 29 0.0 3 0 0 0 29 44 1 0.0 3 0 0 0 1 46 7 0.0 3 0 0 0 7 48 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 69 6 0.0 3 0 0 0 6 70 9 0.0 3 0 0 0 9 73 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 4 0.0 3 0 0 0 4 120 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 1 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 250 1 0.0 3 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 1 1 281 5 0.0 3 0 0 0 5 282 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.92 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 111,311 Reads with adapters: 577 (0.5%) Reads written (passing filters): 111,311 (100.0%) Total basepairs processed: 30,820,308 bp Total written (filtered): 30,818,482 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 577 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.7% C: 12.1% G: 6.6% T: 6.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 495 1739.2 0 495 4 72 434.8 0 72 5 7 108.7 0 7 6 3 27.2 0 3